Short Communication: Accumulation of Neutral Lipids in Liver and Aorta of Nef-Transgenic Mice.


:HIV-infected individuals are at high risk of developing atherosclerosis and cardiovascular disease, in part, due to HIV-induced impairment of cholesterol metabolism. In vitro studies demonstrated that HIV-1 protein Nef inhibits activity of ABCA1, the main cellular cholesterol transporter, leading to cholesterol accumulation in macrophages and conversion of these cells into foam cells, characteristic for atherosclerosis. However, the mechanisms of Nef-mediated effects on cholesterol metabolism in vivo are not well characterized. In this study, we generated Nef-transgenic mice and evaluated the accumulation of neutral lipids in liver and aorta of these animals. Nef expression was low in all transgenic mice, with some mice carrying the Nef transgene, but not expressing the Nef RNA. Using Oil Red O staining, we demonstrated increased levels of neutral lipids in liver and aorta of mice expressing Nef relative to transgenic animals, with no detectable Nef expression or control wild-type mice. These results provide direct evidence that Nef promotes cholesterol deposition in tissues.


Pushkarsky T,Shilov E,Kruglova N,Naumann R,Brichacek B,Jennelle L,Sviridov D,Kruglov A,Nedospasov SA,Bukrinsky M




Has Abstract


2017-01-01 00:00:00












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    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Cilla G,Rodés B,Pérez-Trallero E,Arrizabalaga J,Soriano V

    更新日期:2001-03-20 00:00:00

  • HIV-1 Central Nervous System Compartmentalization and Cytokine Interplay in Non-Subtype B HIV-1 Infections in Nigeria and Malawi.

    abstract::HIV-1 compartmentalization in the central nervous system (CNS) and its contribution to neurological disease have been well documented. Previous studies were conducted among people infected with subtypes B or C where CNS compartmentalization has been observed when comparing viral sequences in the blood to virus in cere...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Adewumi OM,Dukhovlinova E,Shehu NY,Zhou S,Council OD,Akanbi MO,Taiwo B,Ogunniyi A,Robertson K,Kanyama C,Hosseinipour MC,Swanstrom R

    更新日期:2020-06-01 00:00:00

  • Rapid alternation of drug therapy is highly efficient in suppressing the emergence of mutant drug-resistant HIV strains in cell culture.

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    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Balzarini J,De Clercq E

    更新日期:2001-11-20 00:00:00

  • Two Independent HIV Epidemics in Saint Petersburg, Russia Revealed by Molecular Epidemiology.

    abstract::The HIV epidemic in Russia, one of the world's fastest growing, has been concentrated mostly among people who inject drugs (PWID). We sought to explore the epidemiology of the epidemic in St. Petersburg by sampling from the highest risk groups of PWID and men who have sex with men (MSM) and use viral sequencing data t...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Dukhovlinova E,Masharsky A,Toussova O,Verevochkin S,Solovyeva T,Meringof M,Paintsil E,White E,Barbour R,Heimer R,Kozlov A

    更新日期:2015-06-01 00:00:00

  • The Effect of High Rates of Bacterial Sexually Transmitted Infections on HIV Incidence in a Cohort of Black and White Men Who Have Sex with Men in Atlanta, Georgia.

    abstract::Data reporting sexually transmitted infection (STI) incidence rates among HIV-negative U.S. men who have sex with men (MSM) are lacking. In addition, it is difficult to analyze the effect of STI on HIV acquisition given that sexual risk behaviors confound the relationship between bacterial STIs and incident HIV. The I...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Kelley CF,Vaughan AS,Luisi N,Sanchez TH,Salazar LF,Frew PM,Cooper HL,Diclemente R,del Rio C,Sullivan PS,Rosenberg ES

    更新日期:2015-06-01 00:00:00

  • Conserved HIV-1 Gag p24 Epitopes Elicit Cellular Immune Responses That Impact Disease Outcome.

    abstract::Although the breadth of the human immunodeficiency virus type 1 (HIV-1)-specific cellular immune response and its impact on the control of viral replication have already been addressed, reported data have proven controversial. We hypothesize that the nature of targeted epitopes, rather than the simple breadth or magni...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Tarosso LF,Vieira VA,Sauer MM,Tomiyama HI,Kalil J,Kallas EG

    更新日期:2017-08-01 00:00:00

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    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Martini F,D'Offizi G,Girardi E,Vitale A,Cimini E,Mollicone B,Vlassi C,Aiuti F,Other Members of 2005 Italian CD4 Quality Control Network.

    更新日期:2007-06-01 00:00:00

  • Cytokine and growth factor expression by HTLV-1 Lck-tax transgenic cells in SCID mice.

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    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Watters KM,Dean J,Hasegawa H,Sawa H,Hall W,Sheehy N

    更新日期:2010-05-01 00:00:00

  • Neutralization patterns and evolution of sequential HIV type 1 envelope sequences in HIV type 1 subtype B-infected drug-naive individuals.

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    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Nyambi P,Burda S,Urbanski M,Heyndrickx L,Janssens W,Vanham G,Nadas A

    更新日期:2008-12-01 00:00:00

  • Immune restoration by combination of a cytostatic drug (hydroxyurea) and anti-HIV drugs (didanosine and indinavir).

    abstract::Cell activation is essential for HIV infection. CD4+ T lymphocyte activation allows virus replication and CD8+ T lymphocyte activation may contribute to pathogenesis. We combined hydroxyurea, a cytostatic drug that inhibits cell activation and proliferation, with two drugs that inhibit HIV (didanosine and indinavir), ...

    journal_title:AIDS research and human retroviruses

    pub_type: 临床试验,杂志文章


    authors: Lori F,Jessen H,Lieberman J,Clerici M,Tinelli C,Lisziewicz J

    更新日期:1999-05-01 00:00:00

  • Analysis of Clinical HIV-1 Strains with Resistance to Maraviroc Reveals Strain-Specific Resistance Mutations, Variable Degrees of Resistance, and Minimal Cross-Resistance to Other CCR5 Antagonists.

    abstract::Maraviroc (MVC) is an allosteric inhibitor of human immunodeficiency virus type 1 (HIV-1) entry, and is the only CCR5 antagonist licensed for use as an anti-HIV-1 therapeutic. It acts by altering the conformation of the CCR5 extracellular loops, rendering CCR5 unrecognizable by the HIV-1 envelope (Env) glycoproteins. ...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Flynn JK,Ellenberg P,Duncan R,Ellett A,Zhou J,Sterjovski J,Cashin K,Borm K,Gray LR,Lewis M,Jubb B,Westby M,Lee B,Lewin SR,Churchill M,Roche M,Gorry PR

    更新日期:2017-12-01 00:00:00

  • Genetic analysis reveals ongoing HIV type 1 evolution in infected human placental trophoblast.

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    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Zachar V,Zacharova V,Fink T,Thomas RA,King BR,Ebbesen P,Jones TB,Goustin AS

    更新日期:1999-12-10 00:00:00

  • Lymphocyte homeostasis in HIV-infected northern Thais.

    abstract::Cross-sectional laboratory data were used to model the patterns of total lymphocyte count and lymphocyte subpopulation counts among persons with chronic HIV-1 subtype E (CRF01_AE) infection during the 6.5 years preceding death. The data cover 331 HIV-infected decedents from a heterosexual HIV transmission study of 590...

    journal_title:AIDS research and human retroviruses

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    authors: Kingkeow D,Heilig CM,Costello C,Sennun S,Suriyanon V,Rungruengthanakit K,Taejaroenkul S,Nelson KE,Duerr A

    更新日期:2004-06-01 00:00:00

  • HIV type 1 grown on interferon gamma-treated U937 cells shows selective increase in virion-associated intercellular adhesion molecule 1 and HLA-DR and enhanced infectivity for CD4-negative cells.

    abstract::Cellular adhesion molecules, such as ICAM-1, -2, and -3; LFA-1; and HLA class I and II are incorporated into HIV-1 virions during budding from infected cells. These virion-associated molecules can be involved in the adsorption to susceptible cells displaying the corresponding counterligands. A number of cytokines have...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Castilletti C,Capobianchi MR,Fais S,Abbate I,Ficociello B,Ameglio F,Cordiali Fei P,Santini SM,Dianzani F

    更新日期:1995-05-01 00:00:00

  • Neurocognitive impairment and survival in a cohort of HIV-infected patients treated with HAART.

    abstract::Before the introduction of HAART, HIV-associated neurocognitive impairment (NCI) was recognized as an independent risk factor for death. Since 1996, we conducted a prospective study to assess whether NCI still represents a negative prognostic factor for mortality. Patients were administered measures of neurocognitive ...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Tozzi V,Balestra P,Serraino D,Bellagamba R,Corpolongo A,Piselli P,Lorenzini P,Visco-Comandini U,Vlassi C,Quartuccio ME,Giulianelli M,Noto P,Galgani S,Ippolito G,Antinori A,Narciso P

    更新日期:2005-08-01 00:00:00

  • Near full-length genome identification of a novel HIV-1 recombinant form (CRF01_AE/B'/C) among heterosexuals in Jilin, China.

    abstract::Recombinant forms contribute significantly to the genetic diversity of HIV-1. Here we report a novel HIV-1 recombinant form (CRF01_AE/B'/C) detected from a comprehensive HIV-1 molecular epidemiologic study among heterosexuals in Jilin province of northeastern China. Recombinant analyses of the near full-length genome ...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Li X,Ning C,Chen Y,Feng Y,Wei M,Xing H,Shao Y

    更新日期:2014-07-01 00:00:00

  • Regression of feline immunodeficiency virus infection.

    abstract::Regression of feline immunodeficiency virus (FIV) infection was observed in seven of nine vertically infected kittens born to two chronically infected mother cats. Both provirus and nonmaternal FIV antibody were detected in all kittens by 4 weeks of age but only three of the seven kittens were positive by blood mononu...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: O'Neil LL,Burkhard MJ,Obert LA,Hoover EA

    更新日期:1997-05-20 00:00:00

  • Inhibition of HIV type 1 replication by human T lymphotropic virus types 1 and 2 Tax proteins in vitro.

    abstract::Patients with HIV-1 and human T-lymphotropic virus type 2 (HTLV-2) coinfections often exhibit a clinical course similar to that seen in HIV-1-infected individuals who are long-term nonprogressors. These findings have been attributed in part to the ability of HTLV-2 to activate production of antiviral chemokines and to...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Barrios CS,Castillo L,Giam CZ,Wu L,Beilke MA

    更新日期:2013-07-01 00:00:00

  • HIV type 2 Vpx interaction with Gag and incorporation into virus-like particles.

    abstract::The domain of HIV-2 Vpx previously shown to be important for virion incorporation has been mapped to residues 73--89. Mutational analysis of this domain was employed to further define the sequences important for incorporation into virus-like particles, using a vaccinia virus expression system. Deletion of residues 73-...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Jin L,Zhou Y,Ratner L

    更新日期:2001-01-20 00:00:00

  • Impaired Upregulation of the Costimulatory Molecules, CD27 and CD28, on CD4+ T Cells from HIV Patients Receiving ART Is Associated with Poor Proliferative Responses.

    abstract::HIV patients beginning antiretroviral therapy (ART) with advanced immunodeficiency often retain low CD4+ T cell counts despite virological control. We examined proliferative responses and upregulation of costimulatory molecules, following anti-CD3 stimulation, in HIV patients with persistent CD4+ T cell deficiency on ...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Tanaskovic S,Price P,French MA,Fernandez S

    更新日期:2017-02-01 00:00:00

  • Treatment response and drug resistance in patients infected with HIV type 1 group O viruses.

    abstract::Information about the efficacy of antiretroviral drugs in HIV-1 group O strains as well on the virus evolution in terms of resistance development in vivo is very limited. We assessed the clinical, immunological, and virological response to antiretroviral therapy as well as the selection of drug resistance in six HIV-1...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Rodes B,de Mendoza C,Rodgers M,Newell A,Jimenez V,Lopez-Brugada RM,Soriano V

    更新日期:2005-07-01 00:00:00

  • Near full-length genome characterization of an HIV type 1 CRF25_cpx strain from Cameroon.

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    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Luk KC,Holzmayer V,Ndembi N,Swanson P,Brennan CA,Ngansop C,Mbanya D,Kaptué L,Gürtler L,Devare SG,Hackett J

    更新日期:2008-10-01 00:00:00

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    abstract::Low albumin levels have been associated with HIV progression. The objective of this analysis was to confirm this association and to further examine the effect of albumin before and after HIV seroconversion on disease progression. The association was first examined among individuals already infected with HIV at entry i...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Mehta SH,Astemborski J,Sterling TR,Thomas DL,Vlahov D

    更新日期:2006-01-01 00:00:00

  • HTLV type I Tax activation of the CXCR4 promoter by association with nuclear respiratory factor 1.

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    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Moriuchi M,Moriuchi H,Fauci AS

    更新日期:1999-06-10 00:00:00

  • Short communication: the number of HIV major NRTI mutations correlates directly with other antiretroviral-associated mutations and indirectly with replicative capacity and reduced drug susceptibility.

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    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Ross L,Parkin N,Lanier R

    更新日期:2008-04-01 00:00:00

  • Identification of a recombinant HTLV-II envelope protein for serological detection of HTLV-II carriers.

    abstract::To understand the pathogenic potential and the true extent of human T-cell leukemia virus type II (HTLV-II) infection, it is important to develop a specific HTLV-II antigen-based serological test. Plasmid pIIB was constructed and induced in Escherichia coli to express a recombinant protein (RP) containing 140 amino ac...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Chen YM,Essex M

    更新日期:1991-05-01 00:00:00

  • An HIV type 2 case series in Italy: a phylogenetic analysis.

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    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: D'Ettorre G,Lo Presti A,Gori C,Cella E,Bertoli A,Vullo V,Perno CF,Ciotti M,Foley BT,Ciccozzi M,HIV-2 Study Group.

    更新日期:2013-09-01 00:00:00

  • Expression of HIV antigens at the surface of infected T4 cells: immunoelectron microscopic evidence of an immunogenic phase prior to the viral release.

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    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Desportes I,Bonnet D,Nicol I,Snart R,Sarin P

    更新日期:1989-02-01 00:00:00

  • Complexity and dynamics of HIV-1 chemokine receptor usage in a multidrug-resistant adolescent.

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    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Cavarelli M,Mainetti L,Pignataro AR,Bigoloni A,Tolazzi M,Galli A,Nozza S,Castagna A,Sampaolo M,Boeri E,Scarlatti G

    更新日期:2014-12-01 00:00:00

  • Isolation of potent neutralizing monoclonal antibodies from an SIV-Infected rhesus macaque by phage display.

    abstract::The humoral immune response is a mechanism that potently suppresses or prevents viral infections. However, genetic diversity and resistance to antibody-mediated neutralization are serious obstacles in controlling HIV-1 infection. In this study, we isolated monoclonal antibodies from an SIV-infected macaque by using th...

    journal_title:AIDS research and human retroviruses

    pub_type: 杂志文章


    authors: Kuwata T,Katsumata Y,Takaki K,Miura T,Igarashi T

    更新日期:2011-05-01 00:00:00