The Mechanism of Killing by the Proline-Rich Peptide Bac7(1-35) against Clinical Strains of Pseudomonas aeruginosa Differs from That against Other Gram-Negative Bacteria.


:Pseudomonas aeruginosa infections represent a serious threat to worldwide health. Proline-rich antimicrobial peptides (PR-AMPs), a particular group of peptide antibiotics, have demonstrated in vitro activity against P. aeruginosa strains. Here we show that the mammalian PR-AMP Bac7(1-35) is active against some multidrug-resistant cystic fibrosis isolates of P. aeruginosa By confocal microscopy and cytometric analyses, we investigated the mechanism of killing against P. aeruginosa strain PAO1 and three selected isolates, and we observed that the peptide inactivated the target cells by disrupting their cellular membranes. This effect is deeply different from that previously described for PR-AMPs in Escherichia coli and Salmonella enterica serovar Typhimurium, where these peptides act intracellularly after having been internalized by means of the transporter SbmA without membranolytic effects. The heterologous expression of SbmA in PAO1 cells enhanced the internalization of Bac7(1-35) into the cytoplasm, making the bacteria more susceptible to the peptide but at the same time more resistant to the membrane lysis, similarly to what occurs in E. coli The results evidenced a new mechanism of action for PR-AMPs and indicate that Bac7 has multiple and variable modes of action that depend on the characteristics of the different target species and the possibility to be internalized by bacterial transporters. This feature broadens the spectrum of activity of the peptide and makes the development of peptide-resistant bacteria a more difficult process.


Runti G,Benincasa M,Giuffrida G,Devescovi G,Venturi V,Gennaro R,Scocchi M




Has Abstract


2017-03-24 00:00:00












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    authors: McCormick EM,Echols RM

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  • Observational Study of Associations between Voriconazole Therapeutic Drug Monitoring, Toxicity, and Outcome in Liver Transplant Patients.

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    pub_type: 杂志文章


    authors: Hashemizadeh Z,Badiee P,Malekhoseini SA,Raeisi Shahraki H,Geramizadeh B,Montaseri H

    更新日期:2017-11-22 00:00:00

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    authors: Carlisle HN,Saslaw S

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    pub_type: 杂志文章,多中心研究


    authors: Falcone M,Russo A,Shindo Y,Farcomeni A,Pieralli F,Cangemi R,Liu J,Xia J,Okumura J,Sano M,Jones C,Vannucchi V,Mancone M,Micek S,Xu F,Violi F,Kollef M

    更新日期:2019-01-29 00:00:00

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    更新日期:2019-09-23 00:00:00

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    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Fu KP,Neu HC

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  • Evaluation by monte carlo simulation of the pharmacokinetics of two doses of meropenem administered intermittently or as a continuous infusion in healthy volunteers.

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    pub_type: 临床试验,杂志文章,随机对照试验


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    pub_type: 杂志文章


    authors: Biedenbach DJ,Sutton LD,Jones RN

    更新日期:1995-10-01 00:00:00

  • Pharmacokinetics of gatifloxacin in infants and children.

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    pub_type: 临床试验,杂志文章,多中心研究


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    更新日期:2005-03-01 00:00:00

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    pub_type: 杂志文章


    authors: Deng F,Shen J,Zhang M,Wu C,Zhang Q,Wang Y

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    pub_type: 杂志文章


    authors: Groll AH,Sein T,Petraitis V,Petraitiene R,Callender D,Gonzalez CE,Giri N,Bacher J,Piscitelli S,Walsh TJ

    更新日期:1998-10-01 00:00:00

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    pub_type: 杂志文章


    authors: Yeh YC,Chang KC,Yang JC,Fang CT,Wang JT

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    pub_type: 临床试验,杂志文章


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    authors: Wiuff C,Zappala RM,Regoes RR,Garner KN,Baquero F,Levin BR

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    更新日期:1998-04-01 00:00:00

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    authors: Theodos CM,Griffiths JK,D'Onfro J,Fairfield A,Tzipori S

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  • Inhibition of protein synthesis on the ribosome by tildipirosin compared with other veterinary macrolides.

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    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Andersen NM,Poehlsgaard J,Warrass R,Douthwaite S

    更新日期:2012-11-01 00:00:00

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    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章,随机对照试验


    authors: Di Stefano AF,Rusca A,Loprete L,Dröge MJ,Moro L,Assandri A

    更新日期:2011-05-01 00:00:00

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    abstract::Antibiotic dynamics in the fluid obtained from subcutaneously implanted chambers in rats were similar to those observed in serum following single intramuscular injections of carbenicillin. ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Gardner WG,Prior RB,Perkins RL

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  • Risk factors for emergence of resistance to broad-spectrum cephalosporins among Enterobacter spp.

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    authors: Kaye KS,Cosgrove S,Harris A,Eliopoulos GM,Carmeli Y

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    authors: Yi G,Deval J,Fan B,Cai H,Soulard C,Ranjith-Kumar CT,Smith DB,Blatt L,Beigelman L,Kao CC

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    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Pons AM,Zorn N,Vignon D,Delalande F,Van Dorsselaer A,Cottenceau G

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    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Rosenthal KS,Storm DR,Ford WT

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  • Linezolid penetration into bone and joint tissues infected with methicillin-resistant staphylococci.

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    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 临床试验,杂志文章


    authors: Kutscha-Lissberg F,Hebler U,Muhr G,Köller M

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  • Analysis of pH and buffer effects on flucytosine activity in broth dilution susceptibility testing of Candida albicans in two synthetic media.

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    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Calhoun DL,Galgiani JN

    更新日期:1984-09-01 00:00:00

  • Azasordarins: susceptibility of fluconazole-susceptible and fluconazole-resistant clinical isolates of Candida spp. to GW 471558.

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    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Cuenca-Estrella M,Mellado E,Díaz-Guerra TM,Monzón A,Rodríguez-Tudela JL

    更新日期:2001-06-01 00:00:00

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    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Molitoris D,Väisänen ML,Bolaños M,Finegold SM

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    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: van Wijk F,Waterval J,van Aerde K,Henriet SSV,Meijer FJA,Borra LC,Aarnoutse RE,van Ingen J

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    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Spruance SL,Freeman DJ,Sheth NV

    更新日期:1986-07-01 00:00:00

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    pub_type: 杂志文章


    authors: Ranade RM,Zhang Z,Gillespie JR,Shibata S,Verlinde CL,Hol WG,Fan E,Buckner FS

    更新日期:2015-11-01 00:00:00