Yangjing Capsule extract promotes proliferation of GC-1 spg cells via up-regulated POU3F1 pathway.

Abstract:

:As is similar to glial cell line-derived neurotrophic factor (GDNF), the Yangjing Capsule (YC) extract could also lead to proliferation of spermatogonial stem cells (SSCs) by stimulating the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway; however, the regulatory effect of YC extract on the expression of POU3F1 still remains unknown. The objective of this study is to determine whether the transcription factor POU3F1 is up-regulated by YC extract through the PI3K/AKT signaling pathway to regulate SSCs survival and proliferation. Cultured GC-1 spermatogonial (spg) cells were treated with 0.01, 0.1, and 1 mg/mL YC extract for 48 h. Cell viability was analyzed using MTT assay, while POU3F1 expression was quantitatively detected using real time-polymerase chain reaction and Western blot analysis. POU3F1, GDNF family receptor alpha1 (GFRα1) short interfering ribonucleic acid (siRNA), and LY294002 (PI3K inhibitor) were applied as blockers to explore the underlying pathway. After 48 h treatment with YC extract, GC-1 spg cells proliferated and POU3F1 expression was significantly increased in a dose-dependent manner. POU3F1 siRNA partially blocked those effects of YC extract. Both GFRα1 siRNA and LY294002, as upstream blockers, reduced POU3F1 expression induced by YC extract. The conclusion is that YC extract promotes proliferation of GC-1 spg cells via up-regulation of POU3F1. The potential mechanism is that YC extract triggers the activation of the PI3K/AKT pathway and then up-regulates POU3F1 expression.

journal_name

Biosci Trends

journal_title

Bioscience trends

authors

Jin B,Cai B,Sun D,Zhang X,Cui Y,Deng W,Gao C

doi

10.5582/bst.2016.01211

subject

Has Abstract

pub_date

2017-03-22 00:00:00

pages

95-104

issue

1

eissn

1881-7815

issn

1881-7823

journal_volume

11

pub_type

杂志文章
  • Application of low-pressure cell seeding system in tissue engineering.

    abstract::Tissue engineering has been one of the most promising strategies for the regeneration of impaired tissue. Application of three-dimensional porous scaffolds has greatly improved the outcome of tissue engineering in many categories. Cell seeding is one of the key issues in tissue regeneration. It depends not only on the...

    journal_title:Bioscience trends

    pub_type: 杂志文章,评审

    doi:

    authors: Dai W,Dong J,Chen G,Uemura T

    更新日期:2009-12-01 00:00:00

  • Training health professionals to detect and support mothers at risk of postpartum depression or infant abuse in the community: a cross-sectional and a before and after study.

    abstract::The purpose of this study was to evaluate the 2-day seminar in 2 consecutive years respectively for community health professionals. We prepared the child-rearing support manual (CRSM) and conducted a seminar program to improve skills to provide mental assessments and postnatal care support for mothers and their famili...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Kamibeppu K,Furuta M,Yamashita H,Sugishita K,Suzumiya H,Yoshida K

    更新日期:2009-02-01 00:00:00

  • Clinical correspondence to hepatocellular carcinoma-related lesions with atypical radiological pattern.

    abstract::In patients at risk of hepatocarcinogenesis, tumors are frequently detected with atypical radiological patterns related to hepatocellular carcinoma (HCC) on imaging studies. Despite their high potential for malignancy, whether to resect such lesions immediately is controversial. Based on histological findings, patient...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2017.01110

    authors: Higaki T,Midorikawa Y,Nakashima Y,Nakayama H,Matsuoka S,Moriyama M,Sugitani M,Takayama T

    更新日期:2017-07-24 00:00:00

  • Stent placement for benign portal vein stenosis following pancreaticoduodenectomy in a hybrid operating room.

    abstract::Benign portal vein stenosis is a rare complication following pancreaticoduodenectomy. Because a direct surgical approach to the portal vein is difficult due to severe adhesions following pancreaticoduodenectomy, portal vein stent placement is considered a good treatment option. Herein, we report 3 cases of severe port...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2018.01296

    authors: Sawai Y,Kokudo T,Sakamoto Y,Takao H,Kazami Y,Nishioka Y,Akamatsu N,Arita J,Kaneko J,Hasegawa K

    更新日期:2019-01-22 00:00:00

  • Once-daily tacrolimus in living donor liver transplant recipients.

    abstract::Once-daily tacrolimus (denoted here simply as OD) is a recently developed extended release drug formulation. The purpose of the present study was to pharmacokinetically evaluate tacrolimus exposure and determine the feasibility of its de novo use in liver transplant recipients in the perioperative period. This was an ...

    journal_title:Bioscience trends

    pub_type: 临床试验,杂志文章

    doi:10.5582/bst.2011.v5.4.156

    authors: Sugawara Y,Miyata Y,Kaneko J,Tamura S,Aoki T,Sakamoto Y,Hasegawa K,Yamashiki N,Kokudo N

    更新日期:2011-08-01 00:00:00

  • MiR-15a-5p regulates viability and matrix degradation of human osteoarthritis chondrocytes via targeting VEGFA.

    abstract::Previous studies demonstrated that miR-15a-5p was probably associated with human hepatocellular carcinoma, while the function of miR-15a-5p in OA (Osteoarthritis) still remains unknown. Here, we uncovered the potential role of miR-15a-5p on OA pathogenesis and confirmed its predicted target VEGFA (Vascular Endothelial...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2016.01187

    authors: Chen H,Tian Y

    更新日期:2017-01-16 00:00:00

  • Prevalence of HIV infection and HIV-related sex risk behaviors in men who have sex with men in Shandong Province, China.

    abstract::Recently, men who have sex with men (MSM) have become a population at high risk ofHIV infection in China, and more investigations of and intervention programs targeting this hidden population are urgently needed. The objective of this study was to assess the prevalence of HIV-positive individuals, HIV-related knowledg...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Tao X,Gai R,Zhang X,Zhang N,Zheng W,Xu A,Li S

    更新日期:2008-06-01 00:00:00

  • The preliminary clinical observation and analysis of childbearingage women with a history of iodine-131 treatment for Graves' disease.

    abstract::Whether radioactive iodine treatment of Graves' disease (GD) during pregnancy will increase pregnancy loss and affect fetal development is still a matter of concern. From May 2005 to December 2015, 2,276 childbearing-age women with GD received iodine-131 treatment in our departments and were retrospectively enrolled i...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2016.01008

    authors: Guan L,Chen G,Zhang J,Wang L

    更新日期:2016-09-05 00:00:00

  • Research progress on the direct antiviral drugs for hepatitis C virus.

    abstract::Hepatitis C, caused by the hepatitis C virus (HCV) that attacks the liver and leads to inflammation, is a severe threat to human health. Pegylated interferon α (INF-α) and ribavirin based therapy was once the standard therapy for HCV infection. However, it is suboptimal in efficacy and poorly tolerated in some patient...

    journal_title:Bioscience trends

    pub_type: 杂志文章,评审

    doi:10.5582/bst.2016.01266

    authors: Gao J,Ju C

    更新日期:2017-03-22 00:00:00

  • Comparison between endoluminal ultrasonography and spiral computerized tomography for the preoperative local staging of rectal carcinoma.

    abstract::The aim of this study is to compare the efficacy of endoluminal ultrasonography (EUS) and spiral computerized tomography (SCT) in preoperative local staging of rectal carcinoma. EUS and SCT were performed prior to surgery in 78 patients with rectal carcinoma. After radical surgery, the preoperative findings were compa...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Ju H,Xu D,Li D,Chen G,Shao G

    更新日期:2009-04-01 00:00:00

  • Japan's emerging challenge for child abuse: system coordination for early prevention of child abuse is needed.

    abstract::At the end of 2013, a Japanese newspaper reported that 4,173 children were unidentified or missing in Japan. The article concluded that child abuse was a matter of national concern. In examining the strengths and weaknesses of Japan's welfare system in regard to child abuse, it would seem that a weakness exists with r...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Takahashi K,Kanda H,Sugaya N

    更新日期:2014-08-01 00:00:00

  • Progress of research on microRNAs with diagnostic value in asbestos exposure: A call for method standardization.

    abstract::Malignant mesothelioma (MM) is an insidious, lethal asbestos-related cancer that is poorly responsive to current treatments. Specific and sensitive biomarkers providing early MM diagnosis in exposed subjects, who are at high-risk of developing it, are sorely needed. MicroRNAs (miRNAs) are endogenous, non-coding, small...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2016.01249

    authors: Micolucci L,Rippo MR,Olivieri F,Procopio AD

    更新日期:2017-03-22 00:00:00

  • Syndrome-causing mutations in Werner syndrome.

    abstract::Complete loss of function in the WRN: RecQ3 DNA/RNA helicase gene causes Werner Syndrome (WS). WS patients with genetic instability manifest an early onset of age-related diseases including diabetes mellitus (DM), osteoporosis, atherosclerosis, and malignancy as well as early death. In 1,420 patients, WS was reported ...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Goto M

    更新日期:2008-08-01 00:00:00

  • The surgical management of spontaneous esophageal perforation (Boerhaave's syndrome) ‒ 20 years of experience.

    abstract::Spontaneous esophageal perforation (Boerhaave's syndrome) is an uncommon and challenging condition with significant morbidity and mortality. Surgical treatment is indicated in the large majority of cases and different procedures have been described in this respect. We present the results of a mono-institutional evalua...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2016.01009

    authors: Pezzetta E,Kokudo T,Uldry E,Yamaguchi T,Kudo H,Ris HB,Christodoulou M,Vuilleumier H,Halkic N

    更新日期:2016-05-23 00:00:00

  • An ethanol extract of Aster yomena (Kitam.) Honda inhibits lipopolysaccharide-induced inflammatory responses in murine RAW 264.7 macrophages.

    abstract::Aster yomena (Kitam.) Honda has been widely used as a traditional herbal medicine for centuries to treat cough, asthma, insect bites, etc. Recent reports indicate that A. yomena possesses a wide spectrum of pharmacological activities; however, few experiments have described its anti-inflammatory properties. The presen...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2016.01217

    authors: Kang HJ,Jeong JS,Park NJ,Go GB,Kim SO,Park C,Kim BW,Hong SH,Choi YH

    更新日期:2017-03-22 00:00:00

  • Protective effects of luteolin-7-O-glucoside against starvation-induced injury through upregulation of autophagy in H9c2 Cells.

    abstract::Cardiomyocyte nutrient deprivation is a common clinical event that mediates various cardiac ischemic processes and is associated with autophagy activation and cell survival or death. Luteolin-7-O-glucoside (LUTG) was one of the flavonoid glycosides isolated from Dracocephalum tanguticum. Previous research had showed t...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2017.01111

    authors: Yao H,Zhou L,Tang L,Guan Y,Chen S,Zhang Y,Han X

    更新日期:2017-11-20 00:00:00

  • Children with GJB2 gene mutations have various audiological phenotypes.

    abstract::The current study retrospectively investigated variations in audiological phenotypes in children with GJB2 gene mutations. Subjects were 128 infants and young children who were seen as outpatients by Otology at Beijing Tongren Hospital from 2012 to 2018. Of the 128 subjects, 99 had biallelic truncating (T/T) mutations...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2018.01159

    authors: Wang X,Huang L,Zhao X,Wang X,Cheng X,Du Y,Liu D

    更新日期:2018-09-19 00:00:00

  • Experimental study on inhibition of rat ventricular Ik1 by RNA interference targeting the KCNJ2 gene.

    abstract::The dominant-negative inhibition of KCNJ2-encoded inward rectifier potassium channels (Kir2) is currently considered the best approach to biological pacemakers. We hypothesized that inhibition of the inward rectifier potassium current (IK1) in ventricular myocytes by RNA interference (RNAi) would convert ventricular m...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2012.v6.1.26

    authors: Hu B,Zhu XL,Fan QX,Li HX,Zou CW

    更新日期:2012-02-01 00:00:00

  • Relationships between neighborhood attributes and subjective well-being among the Chinese elderly: Data from Shanghai.

    abstract::It has been hypothesized that subjective well-being (SWB) is determined by a combination of individual characteristics, social environment, and physical environment. However, few studies have simultaneously examined the relationships of the social and physical attributes of a neighborhood with SWB. Accordingly, the pr...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2017.01170

    authors: Gao J,Weaver SR,Fu H,Jia Y,Li J

    更新日期:2017-11-20 00:00:00

  • DNA content can improve the detection and prognosis of carcinoma of the cervix.

    abstract::In all malignant diseases, several clinical and histopathological tests are established as standard methods for diagnosis. Alternative diagnostic quantitative methods are still lacking or conflicting in cancer of the cervix. Although DNA analysis was one of the earliest applications in flow cytometry and the DNA conte...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Chhavi,Saxena M,Negi MP,Singh S,Singh PK,Singh U,Bhatt ML

    更新日期:2010-06-01 00:00:00

  • An in vitro study of pcDNA 3.0-hVEGF165 gene transfection in endothelial progenitor cells derived from peripheral blood of rabbits.

    abstract::The present study investigated the effect of the VEGF165 gene on adhesion, migration, and proliferation of endothelial progenitor cells (EPCs) derived from peripheral blood of rabbits. Peripheral blood mononuclear cells were isolated from rabbits by density gradient centrifugation with Ficoll-Plaque Plus. EPCs were ch...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2012.v6.5.270

    authors: Gu X,Miao X,Xu J,Cao J

    更新日期:2012-10-01 00:00:00

  • Dengue causing fulminant hepatitis in a hepatitis B virus carrier.

    abstract::Dengue is an acute febrile illness resulting from infection by a flavivirus transmitted by the Aedes mosquito. It is characterized by bleeding manifestations and a plasma leak syndrome. Hepatic involvement in the form of elevation in transaminases is common. However, acute hepatic failure is uncommon. It is not known ...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2011.v5.1.44

    authors: Agarwal MP,Giri S,Sharma V,Roy U,Gharsangi K

    更新日期:2011-01-01 00:00:00

  • GRIM-19 over-expression represses the proliferation and invasion of orthotopically implanted hepatocarcinoma tumors associated with downregulation of Stat3 signaling.

    abstract::The retinoid-interferon-induced mortality-19 (GRIM-19) gene has been identified as a negative regulator associated with tumor development. The current study created a model of an orthotopically implanted hepatocarcinoma tumor to verify the inhibitory effect of GRIM-19 in vivo. After treatment with GRIM-19 carried by a...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2019.01185

    authors: Kong D,Chen J,Sun X,Lin Y,Du Y,Huang D,Cheng H,He P,Yang L,Wu S,Zhao L,Meng X

    更新日期:2019-01-01 00:00:00

  • Comparison of mechanisms underlying changes in glucose utilization in fasted rats anesthetized with propofol or sevoflurane: Hyperinsulinemia is exaggerated by propofol with concomitant insulin resistance induced by an acute lipid load.

    abstract::The effects of anesthesia with sevoflurane and with propofol on glucose utilization in rats were investigated. Sevoflurane significantly impairs glucose utilization whereas propofol does not. Both insulin secretion and sensitivity affect glucose utilization. Propofol is hydrophobic, and anesthesia with this agent is a...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2014.01060

    authors: Li X,Kitamura T,Kawamura G,Mori Y,Sato K,Araki Y,Sato R,Yamada Y

    更新日期:2014-06-01 00:00:00

  • Identification of mouse mutant cells exhibiting the plastic mutant phenotype.

    abstract::The initial processes involved in radiation carcinogenesis have not been clearly elucidated. We isolated mouse mutant cells exhibiting plasticity in their mutation phenotypes. These mutant cells were originally isolated from an irradiated cell population as 6-thioguanine resistant (6TGR) mutants that were deficient in...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Yamauchi M,Fukutsu K,Sakagami M,Yamada Y

    更新日期:2010-08-01 00:00:00

  • China issues the National Essential Medicines List (2018 edition): Background, differences from previous editions, and potential issues.

    abstract::On October 25, 2018, the National Health Commission of China issued the National Essential Medicines List (2018 edition) [NEML (2018)]. The NEML (2018) contains 685 drugs, which consist of 417 chemicals and biological products and 268 Chinese patent medicines. Compared to the 2012 version of the NEML, a total number o...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2018.01265

    authors: He J,Tang M,Ye Z,Jiang X,Chen D,Song P,Jin C

    更新日期:2018-01-01 00:00:00

  • Traditional Chinese medicine and related active compounds against hepatitis B virus infection.

    abstract::Hepatitis B induced by hepatitis B virus (HBV) remains a major public health problem worldwide. Although several antiviral drugs have been approved for hepatitis B, they cause significant dose-dependent side-effects (interferon-alpha) and drug resistance (lamivudine, etc.). Safe and potent new anti-HBV drugs are urgen...

    journal_title:Bioscience trends

    pub_type: 杂志文章,评审

    doi:

    authors: Cui X,Wang Y,Kokudo N,Fang D,Tang W

    更新日期:2010-04-01 00:00:00

  • N-terminal PEGylation of human serum albumin and investigation of its pharmacokinetics and pulmonary microvascular retention.

    abstract::Human serum albumin (HSA) is used as an important plasma volume expander in clinical practice. In the present study, HSA was N-terminally PEGylated and a PEGylated HAS (PEG-HSA) carrying one chain of PEG (20 kDa) per HSA molecule was obtained. The purity, secondary structure and hydrodynamic radius of the modified pro...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Zhao T,Yang Y,Zong A,Tan H,Song X,Meng S,Song C,Pang G,Wang F

    更新日期:2012-04-01 00:00:00

  • Involvement of the central monoaminergic system in the antidepressant-like effect of catalpol in mice.

    abstract::Catalpol is a natural iridoid glycoside with diverse bioactivities that is found in abundance in Rehmannia glutinosa Libosch. (Scrophulariaceae). The present study assessed whether catalpol treatment (5, 10, or 20 mg/kg for 14 days by intragastric administration (i.g.)) has an antidepressant-like effect on mice perfor...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2014.01029

    authors: Wang J,Cui Y,Feng W,Zhang Y,Wang G,Wang X,Zhou G

    更新日期:2014-10-01 00:00:00

  • Bilirubin effect on endothelial adhesion molecules expression is mediated by the NF-kappaB signaling pathway.

    abstract::We have recently demonstrated that unconjugated bilirubin (UCB) limits the overexpression of adhesion molecules and inhibits the PMN endothelial adhesion induced by the pro-inflammatory cytokine TNFalpha. To understand the molecular events involved we investigated whether the inhibitory effect is determined by a direc...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Mazzone GL,Rigato I,Ostrow JD,Tiribelli C

    更新日期:2009-08-01 00:00:00