Abstract：
:We developed a single-walled carbon nanotubes (SWCNTs)-based electrochemical biosensor for the detection of Bacillus subtilis, one of the microorganisms observed in Asian dust events, which causes respiratory diseases such as asthma and pneumonia. SWCNTs plays the role of a transducer in biological antigen/antibody reaction for the electrical signal while 1-pyrenebutanoic acid succinimidyl ester (1-PBSE) and ant-B. subtilis were performed as a chemical linker and an acceptor, respectively, for the adhesion of target microorganism in the developed biosensor. The detection range (102-1010 CFU/mL) and the detection limit (102 CFU/mL) of the developed biosensor were identified while the response time was 10 min. The amount of target B. subtilis was the highest in the specificity test of the developed biosensor, compared with the other tested microorganisms (Staphylococcus aureus, Flavobacterium psychrolimnae, and Aquabacterium commune). In addition, target B. subtilis detected by the developed biosensor was observed by scanning electron microscope (SEM) analysis.
journal_name
Chemospherejournal_title
Chemosphereauthors
Yoo MS,Shin M,Kim Y,Jang M,Choi YE,Park SJ,Choi J,Lee J,Park Cdoi
10.1016/j.chemosphere.2017.02.060subject
Has Abstractpub_date
2017-05-01 00:00:00pages
269-274eissn
0045-6535issn
1879-1298pii
S0045-6535(17)30238-2journal_volume
175pub_type
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