Improved antiviral efficacy using TALEN-mediated homology directed recombination to introduce artificial primary miRNAs into DNA of hepatitis B virus.

abstract：:Gastrointestinal (GI) anthrax, caused by the bacterial infection of Bacillus anthracis, posts a significant bioterrorism threat by its relatively high mortality rate in humans. Different from inhalational anthrax by the route of infection, accumulating evidence indicates the bypass of vegetative bacteria across GI epi...

journal_title：Biochemical and biophysical research communications

authors： Bishop BL,Lodolce JP,Kolodziej LE,Boone DL,Tang WJ

更新日期：2010-04-02 00:00:00

abstract：:In rodents, the transcription factors early growth response 2 (EGR2) and sterol regulatory element binding protein 1a (SREBP1a) regulate transcription of the stearoyl-CoA desaturase 2 (SCD2) gene during peripheral nerve myelination, which may be important for synthesis of the lipid component of myelin. Most non-rodent...

journal_title：Biochemical and biophysical research communications

authors： Lengi AJ,Corl BA

更新日期：2012-05-04 00:00:00

abstract：:The protein kinase CK2 holoenzyme is composed of two regulatory beta- and two catalytic alpha- or alpha(')-subunits. There is ample evidence for the binding of individual subunits of CK2 to various cellular proteins and, moreover, for functions of the individual subunits, which are different from their roles in the ho...

journal_title：Biochemical and biophysical research communications

authors： Faust M,Kartarius S,Schwindling SL,Montenarh M

更新日期：2002-08-09 00:00:00

abstract：:In cultures of human foreskin fibroblasts most of the cell surface binding sites for 2-chain urokinase are masked and can be exposed by 10 min. incubation on ice at pH 2.5 (A. Bajpai and J.B. Baker (1985), Biochem. Biophys. Res. Commun.133, 475-482). Here we show that incubation on ice at pH 2.5 also releases from the...

journal_title：Biochemical and biophysical research communications

authors： Bajpai A,Baker JB

更新日期：1985-12-31 00:00:00

abstract：:The effect of vitamin A supplementation on stearoyl-CoA desaturase gene 1 expression in mouse liver was characterized. Normal BALB/c mice were fed 0.01% and 0.1% retinol palmitate as components of nonpurified diets. This treatment resulted in a 3-fold and a 7-fold induction of SCD1 mRNA levels, respectively, as determ...

journal_title：Biochemical and biophysical research communications

authors： Miller CW,Waters KM,Ntambi JM

更新日期：1997-02-03 00:00:00

abstract：:We constructed a system for expressing the Fab of the therapeutic human monoclonal antibody adalimumab at a yield of 20 mg/L in the methylotrophic yeast Pichia pastoris. To examine the contribution of interchain disulfide bonds to conformational stability, we prepared adalimumab Fab from which the interchain disulfide...

journal_title：Biochemical and biophysical research communications

authors： Nakamura H,Oda-Ueda N,Ueda T,Ohkuri T

更新日期：2018-01-01 00:00:00

abstract：:Plant innate immunity varies with age and plant developmental stages. Recently, we reported that Arabidopsis thaliana microRNA miR172b regulates FLS2 transcription through two transcription factors: TARGET OF EAT1 (TOE1) and TOE2. Although the flg22-triggered immune responses were investigated in 2-d-old or even young...

journal_title：Biochemical and biophysical research communications

authors： Zou Y,Wang S,Lu D

更新日期：2020-10-22 00:00:00

abstract：:Reactive oxygen species (ROS) are a continuous hazard in eukaroytic cells by their ability to cause damage to biomolecules, in particular to DNA. Previous data indicated that the cytosolic serine peptidase tripeptidyl-peptidase II (TPPII) translocates into the nucleus of most tumor cell lines in response to gamma-irra...

journal_title：Biochemical and biophysical research communications

authors： Preta G,de Klark R,Chakraborti S,Glas R

更新日期：2010-08-27 00:00:00

abstract：:Mechanical loading of cultured mesangial cells has been shown to induce both increased production of matrix proteins and cell proliferation. This in vitro phenomenon has been implicated in the progression of glomerular sclerosis observed in a variety of glomerular diseases. However, it is not yet known how cells sense...

journal_title：Biochemical and biophysical research communications

authors： Hamasaki K,Mimura T,Furuya H,Morino N,Yamazaki T,Komuro I,Yazaki Y,Nojima Y

更新日期：1995-07-17 00:00:00

abstract：:The alpha-silyl amines benzyl-dimethyl-silyl-methanamine and the p-fluoro and p-chloro derivatives are potent time-dependent inhibitors of rat brain MAO-B. The inhibition exhibits saturation kinetics, takes place in the enzyme active-site and is irreversible. The most potent inhibitor in the series is 4-fluorobenzyl-d...

journal_title：Biochemical and biophysical research communications

authors： Danzin C,Collard JN,Marchal P,Schirlin D

更新日期：1989-04-28 00:00:00

abstract：:Gastric inhibitory polypeptide (GIP) is released from the small intestine upon meal ingestion and increases insulin secretion from pancreatic β cells. Although the GIP receptor is known to be expressed in small intestine, the effects of GIP in small intestine are not fully understood. This study was designed to clarif...

journal_title：Biochemical and biophysical research communications

authors： Ogawa E,Hosokawa M,Harada N,Yamane S,Hamasaki A,Toyoda K,Fujimoto S,Fujita Y,Fukuda K,Tsukiyama K,Yamada Y,Seino Y,Inagaki N

更新日期：2011-01-07 00:00:00

abstract：PURPOSE:Cancer stem cells (CSCs), also known as tumor-initiating cells, are involved in tumor progression, metastasis, and drug resistance. Hybrid liposomes (HLs) are nano-sized liposomal particles that can be easily prepared by ultrasonicating a mixture of vesicular and micellar molecules in buffer solutions. In this ...

journal_title：Biochemical and biophysical research communications

authors： Inamura K,Komizu Y,Yamakuchi M,Ishida S,Matsumoto Y,Matsushita T

更新日期：2019-01-29 00:00:00

abstract：:The effects of pioglitazone, a novel antidiabetic insulin-sensitizing agent, on insulin signaling in skeletal muscles from genetically obese Wistar fatty rats and their lean littermates were studied. Increased tyrosine phosphorylation levels of insulin receptors (IRs) and insulin receptor substrate 1 (IRS-1) in respon...

journal_title：Biochemical and biophysical research communications

authors： Hayakawa T,Shiraki T,Morimoto T,Shii K,Ikeda H

更新日期：1996-06-14 00:00:00

abstract：:We have identified two types of homologous DNA pairing activity in mouse cell extracts by a strand-transfer assay. Both activities are separated from each other by anion-exchange chromatography; neither of them needs ATP. One requires magnesium ion and is stimulated by Escherichia coli single-stranded DNA binding prot...

journal_title：Biochemical and biophysical research communications

authors： Mishina Y,Shimokawa T,Kawasaki K,Arai N,Shibata T,Koyama H

更新日期：1992-06-30 00:00:00

abstract：:A variety of different forms of cellular stress can cause protein misfolding and aggregation and proteotoxicity. The cytoprotective response to proteotoxicity is termed the integrated stress response and involves 4 distinct serine/threonine protein kinases that converge on the translation initiation factor eIF2α, resu...

journal_title：Biochemical and biophysical research communications

authors： McConkey DJ

更新日期：2017-01-15 00:00:00

abstract：:To study its biological functions, tumor necrosis factor precursor (proTNF) with a molecular size of 26-KDa was obtained as a recombinant protein from Escherichia coli. The recombinant proTNF was successfully accumulated in the insoluble form, corresponding to about 10-15% of total E. coli proteins. Solubilization, ge...

journal_title：Biochemical and biophysical research communications

authors： Tanabe Y,Noguchi K,Morikawa A,Mizuno D,Soma G

更新日期：1991-08-30 00:00:00

abstract：OBJECTIVE:Screening and identifying the gene mutation of EXT1, EXT2 and EXT3 associated with multiple exostosis (ME) and the expression in tumor tissues. METHODS:Nine patients with multiple exostosis were collected and genomic DNA was extracted. Polymerase chain reaction (PCR) amplification and direct sequencing techn...

journal_title：Biochemical and biophysical research communications

authors： Wu ZY,Wang Y,Wang JW,Chen YZ,Guo Y

更新日期：2018-11-10 00:00:00

abstract：:The effect that several substances may have on ANF release by atrial slices and on its tissular content was investigated. alpha- and beta-adrenergic and cholinergic agonists, vasopressin, met-enkephaline, dexamethasone and DOC, in concentration ranging from 10(-4) to 10(-8) M, were added into the incubation media and ...

journal_title：Biochemical and biophysical research communications

authors： Lachance D,Garcia R,Gutkowska J,Cantin M,Thibault G

更新日期：1986-03-28 00:00:00

abstract：:p73, a member of the p53 family, is a transcription factor that plays a key role in many biological processes. In the present study, we show that TAp73 is expressed in neural stem cells (NSC) and its expression increases following their differentiation. NSC from p73 null mice have a reduced proliferative potential, to...

journal_title：Biochemical and biophysical research communications

authors： Agostini M,Tucci P,Chen H,Knight RA,Bano D,Nicotera P,McKeon F,Melino G

更新日期：2010-12-03 00:00:00

abstract：:Leucine-rich repeat kinase 2 (LRRK2) is the causal gene for autosomal dominant familial Parkinson's disease. We have previously reported a novel molecular feature characteristic to I2020T mutant LRRK2: higher susceptibility to post-translational degradation than the wild-type LRRK2. In the present study, we demonstrat...

journal_title：Biochemical and biophysical research communications

authors： Ohta E,Kubo M,Obata F

更新日期：2010-01-01 00:00:00

abstract：:Human endothelial cell thrombin receptors were functionally expressed in Xenopus laevis oocytes by injection of RNA extracted from human umbilical vein endothelial cells. Oocytes injected with endothelial cell RNA responded to thrombin with a Ca2(+)-dependent depolarizing current whose size depended on the amount of R...

journal_title：Biochemical and biophysical research communications

authors： Pipili-Synetos E,Gershengorn MC,Jaffe EA

更新日期：1990-09-28 00:00:00

abstract：:Tat-interactive protein, 60kDa (Tip60) is a histone acetyltransferase with specificity toward lysine 5 of histone H2A (H2AK5) and plays multiple roles in chromatin remodeling processes. Co-immunoprecipitation experiments performed on Jurkat cells, showed that Tip60 is present in the same macro-molecular complex as UHR...

journal_title：Biochemical and biophysical research communications

authors： Achour M,Fuhrmann G,Alhosin M,Rondé P,Chataigneau T,Mousli M,Schini-Kerth VB,Bronner C

更新日期：2009-12-18 00:00:00

abstract：:Previously, we have investigated the potential for a pro-oxidant interaction of iron and ascorbate in vivo in iron and ascorbate cosupplementation or ascorbate supplementation studies. In this study, for the first time, the effects of iron supplementation on oxidative damage to DNA in healthy individuals with plasma a...

journal_title：Biochemical and biophysical research communications

authors： Proteggente AR,England TG,Rice-Evans CA,Halliwell B

更新日期：2001-10-19 00:00:00

abstract：:We designed a screen to identify starfish oocyte proteins able to bind monomeric cyclin B by affinity chromatography on a cyclin B splice variant displaying low affinity for cdc2. We identified a 15kDa protein previously described as a cdk-binding protein [Biochim. Biophys. Acta Mol. Cell Res. 1589 (2002) 219-231]. Cy...

journal_title：Biochemical and biophysical research communications

authors： Offner N,Derancourt J,Lozano JC,Schatt P,Picard A,Peaucellier G

更新日期：2003-01-03 00:00:00

abstract：:Recently, the concern that toluene might have carcinogenic and reproductive toxic potential has been raised. We investigated the ability of DNA damage by minor metabolites of toluene, methylhydroquinone, and methylbenzoquinone, using (32)P-5'-end-labeled DNA fragments obtained from the human genes. Methylhydroquinone ...

journal_title：Biochemical and biophysical research communications

authors： Murata M,Tsujikawa M,Kawanishi S

更新日期：1999-08-02 00:00:00

abstract：:Acyl-CoA dehydrogenases (ACADs) are a family of mitochondrial enzymes catalyzing the initial rate-limiting step in the beta-oxidation of fatty acyl-CoA. The reaction provides main source of energy for human heart and skeletal muscle. Eight human ACADs have been described. Deficiency of these enzymes, especially very l...

journal_title：Biochemical and biophysical research communications

authors： Zhang J,Zhang W,Zou D,Chen G,Wan T,Zhang M,Cao X

更新日期：2002-10-04 00:00:00

abstract：:Glycolysis, the catabolism of glucose to pyruvate, is an iconic central metabolic pathway and often used as a paradigm for explaining the general principles of the regulation/control of cellular metabolism. The ubiquitous mammalian ATP-dependent hexokinases I-III and hexokinase IV, also termed glucokinase, initiate th...

journal_title：Biochemical and biophysical research communications

authors： Ronimus RS,Morgan HW

更新日期：2004-03-12 00:00:00

abstract：:Anchoring protein alphaKAP targets calmodulin kinase II (CaMKII) to the sarcoplasmic reticulum (SR), and in the rabbit is a substrate of CaMKII itself in fast-twitch, but not in slow-twitch muscle. This work was aimed at elucidating the molecular basis for differential phosphorylation of alphaKAP. Here we show that tw...

journal_title：Biochemical and biophysical research communications

authors： Damiani E,Sacchetto R,Salviati L,Margreth A

更新日期：2003-02-28 00:00:00

abstract：:Extracellular acidic pH-activated chloride channel I(Cl, acid), has been characterized in HEK 293 cells and mammalian cardiac myocytes. This study was designed to characterize I(Cl,acid) in human umbilical vein endothelial cells(HUVECs). The activation and deactivation of the current rapidly and repeatedly follows the...

journal_title：Biochemical and biophysical research communications

authors： Ma ZY,Zhang W,Chen L,Wang R,Kan XH,Sun GZ,Liu CX,Li L,Zhang Y

更新日期：2008-07-04 00:00:00

abstract：:Despite their advantages in analysis, 4D NMR experiments are still infrequently used as a routine tool in protein NMR projects due to the long duration of the measurement and limited digital resolution. Recently, new acquisition techniques for speeding up multidimensional NMR experiments, such as nonlinear sampling, i...

journal_title：Biochemical and biophysical research communications

authors： Shigemitsu Y,Ikeya T,Yamamoto A,Tsuchie Y,Mishima M,Smith BO,Güntert P,Ito Y

更新日期：2015-02-06 00:00:00