Abstract:
BACKGROUND:Only a small proportion of patients respond to anti-VEGF therapy, pressing the need for a reliable biomarker that can identify patients who will benefit. We studied the biological activity of anti-VEGF antibodies in patients' blood during anti-VEGF therapy by using the Ba/F3-VEGFR2 cell line, which is dependent on VEGF for its growth. METHODS:Serum samples from 22 patients with cancer before and during treatment with bevacizumab were tested for their effect on proliferation of Ba/F3-VEGFR2 cells. Vascular endothelial growth factor as well as bevacizumab concentrations in serum samples from these patients were determined by enzyme linked immunosorbent assay (ELISA). RESULTS:The hVEGF-driven cell proliferation was effectively blocked by bevacizumab (IC50 3.7 μg ml-1; 95% CI 1.7-8.3 μg ml-1). Cell proliferation was significantly reduced when patients' serum during treatment with bevacizumab was added (22-103% inhibition compared with pre-treatment). Although bevacizumab levels were not related, on-treatment serum VEGF levels were correlated with Ba/F3-VEGFR2 cell proliferation. CONCLUSIONS:We found that the neutralising effect of anti-VEGF antibody therapy on the biological activity of circulating VEGF can be accurately determined with a Ba/F3-VEGFR2 bioassay. The value of this bioassay to predict clinical benefit of anti-VEGF antibody therapy needs further clinical evaluation in a larger randomised cohort.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Wentink MQ,Broxterman HJ,Lam SW,Boven E,Walraven M,Griffioen AW,Pili R,van der Vliet HJ,de Gruijl TD,Verheul HMdoi
10.1038/bjc.2016.275subject
Has Abstractpub_date
2016-10-11 00:00:00pages
940-948issue
8eissn
0007-0920issn
1532-1827pii
bjc2016275journal_volume
115pub_type
杂志文章abstract::Obesity has been associated with an increased risk of renal cell cancer among women, while the evidence for men is considered weaker. We conducted a quantitative summary analysis to evaluate the existing evidence that obesity increases the risk of renal cell cancer both among men and women. We identified all studies e...
journal_title:British journal of cancer
pub_type: 杂志文章,meta分析
doi:10.1054/bjoc.2001.2040
更新日期:2001-09-28 00:00:00
abstract::Gap junctions provide a route for small molecules to pass directly between cells. Toxic species may spread through junctions into 'bystander' cells, which may be exploited in chemotherapy and radiotherapy. However, this may be prevented by junction closure, and therefore an understanding of the dose-dependency of inhi...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601686
更新日期:2004-04-05 00:00:00
abstract::There is evidence that patients living in outlying areas have poorer survival from cancer. This study set out to investigate whether they have more advanced disease at diagnosis. Case notes of 1323 patients in north and northeast Scotland who were diagnosed with lung or colorectal cancer in 1995 or 1996 were reviewed....
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.2000.1708
更新日期:2001-04-06 00:00:00
abstract::Ovarian cancer is the fifth leading cause of female cancer deaths in the Western world. Significant progress has been made in the treatment of patients with ovarian cancer, however, the majority of patients experience disease recurrence and new therapies are being sought for such patients. Clinical investigation of po...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/bjc.2015.395
更新日期:2015-12-15 00:00:00
abstract::The orally administered tubulin-binding agent 1069C85 was developed with the hope of overcoming the multidrug resistance associated with existing anti-tubulin agents, such as the vinca alkaloids. A phase I study was performed using a single oral dose every 3 weeks, administered as a suspension reconstituted in 0.1% Tw...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/bjc.1997.107
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Aspirin use has been shown to lower incidence and mortality in cancer patients. The aim of this population-based study was to determine the effect of postdiagnosis low-dose aspirin use on survival of patients with oesophageal cancer. METHODS:Patients with oesophageal cancer (1998-2010) were selected from th...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2016.65
更新日期:2016-04-26 00:00:00
abstract::Between 1980 and 1985, 914 patients with head and neck squamous cell carcinoma underwent lymph node dissection in our institution. The prognostic value of clinical factors has already been reported (Mamelle et al, 1994, Am J Surg 168: 494-498). We present here a comparison of biological characteristics of pharyngeal t...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.320
更新日期:1998-06-01 00:00:00
abstract::Cyclin D1 gene amplification is an important event in many cancers, but it is rarely found in non-small-cell lung cancer (NSCLC). This study was conducted in an attempt to clarify any other mechanisms related to cyclin D1 involvement in the malignant transformation of NSCLC, and we clearly showed for the first time th...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690752
更新日期:1999-10-01 00:00:00
abstract::A CD34-negative haematopoietic progenitor cell line, D064, derived from canine bone marrow stromal cells is able to differentiate into haematopoietic progenitors under the influence of growth factor-mediated signalling. While differentiating, these cells eventually start to express MHC class II molecules (DR homologue...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690768
更新日期:1999-11-01 00:00:00
abstract::A case-control study was conducted in Italy and Switzerland between 1992 and 2000 on 527 cases of laryngeal cancer and 1,297 hospital controls. The risk of laryngeal cancer steadily decreased from 3 years after stopping smoking. Some decline in risk was observed only 20 years or more after stopping drinking. ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600638
更新日期:2002-11-18 00:00:00
abstract:BACKGROUND:We previously showed that activation of the nuclear factor of activated T cells (NFAT)1/Fas ligand (FasL) pathway induces glioma cell death. Lithium (Li) is an inhibitor of glycogen synthase kinase (GSK)-3 that activates NFAT1/FasL signalling. Temozolomide (TMZ) inhibits GSK-3 and activates Fas in tumour pro...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2017.89
更新日期:2017-05-09 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the most malignant human tumours because of its high incidence of metastasis. The mechanisms underlying the metastasis of HCC, however, remain poorly understood. In this study, we performed cDNA microarray analysis to profile gene expression patterns in two subtypes of HCC, sol...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601749
更新日期:2004-06-14 00:00:00
abstract::Estimation of FGF-3 oncogene amplification in DNA samples extracted from paraffin embedded sections of 136 ovarian cancer samples was carried out by a quantitative PCR method. The aim of this study was to elucidate a possible association of FGF-3 copy numbers with established prognostic factors such as age, histology,...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1993.206
更新日期:1993-05-01 00:00:00
abstract::Survivin is an apoptotic inhibitor that is expressed at high levels in a variety of malignancies. Survivin has four known alternative splice forms (Survivin, Survivin-2B, Survivin-deltaEx3, and Survivin-3B), and the recent literature suggests that these splice variants have unique functions and subcellular localisatio...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602317
更新日期:2005-01-31 00:00:00
abstract::A brief review is presented of the evidence linking the development of certain types of neoplasms, and of the malignant lymphomas in particular, to chronic immunosuppression in animals and man and to the naturally occurring human immunologic deficiency states. The discussion then focuses on Hodgkin's disease and consi...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/bjc.1971.78
更新日期:1971-12-01 00:00:00
abstract:BACKGROUND:Sequence variations in coding and non-coding regions of the genome can affect gene expression and signalling pathways, which in turn may influence disease outcome. METHODS:In this study, we integrated somatic mutations, gene expression and clinical data from 930 breast cancer patients included in the TCGA d...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-018-0030-0
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:Eukaryotic translation elongation factor 1A2 (eEF1A2) is a known proto-oncogene. We proposed that stimulation of the eEF1A2 expression in cancer tissues is caused by the loss of miRNA-mediated control. METHODS:Impact of miRNAs on eEF1A2 at the mRNA and protein levels was examined by qPCR and western blot, r...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.243
更新日期:2013-06-11 00:00:00
abstract::Four-hundred-forty-five husbands of women with invasive cervical carcinoma, 165 of women with in situ cervical cancer, and 717 of control women (age range 19-82 years) were interviewed and a sample of exfoliated cells from the penis obtained in seven case-control studies conducted by the International Agency for Resea...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600194
更新日期:2002-03-04 00:00:00
abstract::Treatment of ex-breeder male NMRI mice with lipid mobilising factor isolated from the urine of cachectic cancer patients, caused a significant increase in glucose oxidation to CO2 compared with control mice receiving phosphate buffered saline. Glucose utilisation by various tissues was determined by the 2-deoxyglucose...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600493
更新日期:2002-08-27 00:00:00
abstract:BACKGROUND:Interferon (IFN)-based therapies could eradicate hepatitis C (HCV) and reduce the risk of hepatocellular carcinoma (HCC). However, HCC could still happen after sustained virological response (SVR). We aimed to develop a simple scoring system to predict the risk of HCC development among HCV patients after ant...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.564
更新日期:2013-10-29 00:00:00
abstract::Because the focus of nasopharyngeal carcinoma (NPC) is very close to intracranial organs, it often makes incursions into cranial cavity. Identification of intracranial invasion-associated indicators will provide potential therapeutic targets for NPC patients with intracranial invasion. In this regard, Human Xpro HC-pl...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604167
更新日期:2008-01-29 00:00:00
abstract::A human prostate tumour cell line, LNCaP C4-2, when injected into athymic male nude mice, produced tumours containing: (1) only human cancer cells similar to those injected; (2) only murine stromal cells containing abnormal chromosome constitutions; or (3) both human prostate cancer cells similar to those injected and...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.524
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Clear cell renal cancer frequently harbours von Hippel-Lindau (VHL) gene mutations, leading to stabilisation of the hypoxia-inducible factors (HIFs) and expression of their target genes. We investigated HIF-1 and HIF-2 in the regulation of microRNA-210 (miR-210), and its clinical relevance in renal tumours. ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.56
更新日期:2013-03-19 00:00:00
abstract::Seventy-one patients suffering from acute myeloid leukaemia (AML) who were already in complete remission and had already received one further course of cytotoxic drugs as consolidation therapy were randomised to receive maintenance chemotherapy alone or the same maintenance chemotherapy plus immunotherapy with BCG and...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1038/bjc.1978.1
更新日期:1978-01-01 00:00:00
abstract::Previous studies have suggested that infant vaccinations may reduce the risk of subsequent childhood leukaemia. Vaccination histories were compared in 439 children (ages 0-14) diagnosed with acute lymphoblastic leukaemia (ALL) in nine Midwestern and Mid-Atlantic states (USA) between 1 January 1989 and 30 June 1993 and...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690668
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND:MiR-125b has critical role in non-small-cell lung cancer (NSCLC) cell migration, and its target genes have not been elucidated. Kinesin-1 light chain (KLC)-2 was predicted as one of miR-125b's targets by bioinformatics analysis. This study is to identify the function of KLC2 and its interaction with miR-125b...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2015.3
更新日期:2015-03-03 00:00:00
abstract::Oestrogen receptor alpha (ER-alpha) gene has two specific promoters, distal (P0) and proximal (P1), which induce almost identical transcripts in size due to different splicing. We examined the methylation at both promoter regions of the ER-alpha gene using HpaII, a methylation-sensitive restriction enzyme, prior to po...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690631
更新日期:1999-08-01 00:00:00
abstract::Darbepoetin alfa (Aranesp), Amgen) is a novel erythropoiesis-stimulating protein with a serum half-life longer than recombinant human erythropoietin (Epo), used in the treatment of cancer-associated anaemia. Anaemia is known to adversely affect prognosis and response to treatment in cancer patients. Solid tumours cont...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602685
更新日期:2005-07-25 00:00:00
abstract::Exposure to the fibrous mineral erionite is known to induce mesothelioma in man and laboratory animals. Previous studies demonstrated the presence of a trypsin-like protease associated with tumour cells. This protease could be demonstrated by the use of fluorescent probes which located cells possessing this enzyme. We...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1988.269
更新日期:1988-11-01 00:00:00
abstract::Hyperthermia (42° C.) exerted an inhibitory effect on the O(2) uptake of rabbit VX2 carcinoma cells in vitro, and led to a decrease in viability and growth potential of the cells, as measured by their ability to produce tumours in rabbits. Anaerobic glycolysis of the tumour cells was unaltered by hyperthermia. Respira...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1971.91
更新日期:1971-12-01 00:00:00