Robust quantitation of basic-protein higher-order aggregates using size-exclusion chromatography.

Abstract:

:Detection of higher-order aggregates (HOA) using size-exclusion chromatography (SEC) was found to be variable for a basic protein, using exposed-silanol or diol-silica-based SEC columns. Preparations of the tetrameric biopharmaceutical enzyme Erwinia chrysanthemil-asparaginase (ErA), which has an isoelectric point of 8.6, were analysed using a diol-silica SEC column. Although the proportions of ErA main peak and octamer species were unaffected, HOA recovery and detection were extremely variable and had poor agreement with an orthogonal measurement technique, analytical ultracentrifugation (AUC). The observation that only HOA was selectively affected by non-specific silanol interactions was unexpected, so alternatives were sought. Coated-silica SEC columns improved the resolution and reproducibility of HOA detection for this alkaline-pI protein, and improved the agreement of HOA with the AUC method. Basic proteins, such as ErA, should be thoroughly evaluated in SEC method development, to ensure that resolution of larger aggregate species is not compromised.

journal_name

J Pharm Biomed Anal

authors

Gervais D,Downer A,King D,Kanda P,Foote N,Smith S

doi

10.1016/j.jpba.2017.03.004

subject

Has Abstract

pub_date

2017-05-30 00:00:00

pages

215-220

eissn

0731-7085

issn

1873-264X

pii

S0731-7085(16)31455-8

journal_volume

139

pub_type

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