Abstract:
:Functionalized collagen-mimetic peptides (CMPs) have been widely used in the preparation of collagen-related biomaterials. Among the reported results, the induced noncovalent interactions between the implanted functional groups or moieties were frequently the key elements to promote the self-assembly of small CMPs. In this work, we designed and synthesized a series of glycosylated CMPs in which 4-O-[β-D-galactopyranosyl]-(2S,4R)-4-hydroxyproline (Hyp(Gal)) was incorporated to explore the effects of glycosylation on the stability and assembly of collagen triple helices. Circular dichroism measurements showed that glycosylation of hydroxyproline slightly destabilized the collagen triple helices, but did not reduce their refolding rate. Compared to non-glycosylated CMPs, the incorporation of Hyp(Gal) speeded up the assembly of CMPs, indicating that this modification could assist the self-assembly of CMPs into higher-order structures, such as fibrils. O-Galactosylation of hydroxyproline imposes contrary effects on the triple helix stability and the self-assembly rate of collagen triple helices, exhibiting a piece of important and useful information for designing collagen-related biomaterials. Our finding also suggests that instead of stabilizing the triple helical conformation of CMPs, installing additional forces between CMPs could be a crucial factor to promote the assembly of CMPs into large-scale constructs.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Huang PW,Chang JM,Horng JCdoi
10.1007/s00726-016-2312-2subject
Has Abstractpub_date
2016-12-01 00:00:00pages
2765-2772issue
12eissn
0939-4451issn
1438-2199pii
10.1007/s00726-016-2312-2journal_volume
48pub_type
杂志文章相关文献
AMINO ACIDS文献大全abstract::In this paper we report the complete separation of amino acids as DABS-derivatives using a 3µm Supelcosil LC-18 (25 cm × 2.1 mm I.D.) narrowbore column. The system described makes it possible to perform the analysis of DABS-amino acids with a sensitivity to the femtomole level. We have also studied the conditions nece...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806005
更新日期:1992-10-01 00:00:00
abstract::The isolated single organotypic slice model allows to investigate the effects of drugs and toxins on the expression of transcription factors in the striatum without dopaminergic and glutamatergic interactions. In this study the effects of margatoxin and iberiotoxin on the expression of c-fos mRNA by in situ hybridizat...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s007260070030
更新日期:2000-01-01 00:00:00
abstract::Polyamines have been related to the "Crassulacean Acid Metabolism" (CAM) in higher plants. Such relationship was however observed in plants where CAM activity is inducible by external factors. Results presented here indicate that, inOpuntia F. indica, cladodes where onset of CAM is dependent on internal conditions, i....
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00814160
更新日期:1994-06-01 00:00:00
abstract::Substance P (SP) is one of the most abundant peptides in the central nervous system and has been implicated in a variety of physiological and pathophysiological processes including stress regulation, as well as affective and anxiety-related behaviour. Consistent with these functions, SP and its preferred neurokinin 1 ...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-006-0335-9
更新日期:2006-10-01 00:00:00
abstract::Glutaryl-CoA dehydrogenase deficiency (GDD), which is one of the most frequent organic acid disorders, is characterized by a specific age- and regional-dependent neuropathology. We hypothesized that the distinct brain damage in GDD could be caused by the main pathologic metabolites, the organic acids glutaric (GA) and...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s007260050003
更新日期:2000-01-01 00:00:00
abstract::The transient receptor potential channels TRPV2 and TRPV5 belong to the vanilloid TRP subfamily. TRPV2 is highly similar to TRPV1 and shares many common properties with it. TRPV5 (and also its homolog TRPV6) is a rather distinct member of the TRPV subfamily. It is distant for being strictly Ca(2+)-selective and featur...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0712-2
更新日期:2011-02-01 00:00:00
abstract::Recovery of amino acid (AA) metabolism and the associated clinical implications in chronic hepatitis C (CHC) patients with sustained virological response (SVR) following anti-hepatitis C virus (HCV) therapy remains elusive. A prospective cohort study was conducted on 222 CHC patients with SVR. Eighty-two age-matched m...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-016-2360-7
更新日期:2017-02-01 00:00:00
abstract::We proposed that the combination of conjugated linoleic acid (CLA) and arginine would decrease adiposity by depressing lipid synthesis in liver and adipose tissues of growing pigs. Pigs were allotted to treatments in a 2 × 2 factorial design with two lipids (CLA or canola oil) and two amino acids [L-arginine or L-alan...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1255-5
更新日期:2012-10-01 00:00:00
abstract::Granulocyte-colony stimulating factor stimulates production and antibacterial function of neutrophiles. Therapy using the recombinant protein drug represents a major step forward in oncology. The protein has not been, however, completely sequenced at the protein level and this formed the rationale of the current study...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0312-1
更新日期:2010-04-01 00:00:00
abstract::Minerals more readily adsorb amino acids with charged R groups than uncharged R groups, so that the incorporation of amino acids with charged R groups into peptides would be more frequent than for amino acids with uncharged R groups. However, 74% of the amino acids in the proteins of modern organisms contain uncharged...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-004-0106-4
更新日期:2004-08-01 00:00:00
abstract::The presence of an enzyme activity which hydrolyzes glycyl-D-aspartate was found in the homogenates of pig kidney cortex. The activity was inhibited by metal chelating agents and cilastatin, suggesting that the enzyme was a cilastatin-sensitive metallo-peptidase. Of the two hydrolysis products,D-aspartate was found to...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806591
更新日期:1996-06-01 00:00:00
abstract::Elevated plasma concentrations of asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) were found in various clinical settings including coronary heart disease. To assess ADMA and SDMA diagnostic validity in patients with different stages of ischemic heart disease, we studied these markers in patie...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1307-x
更新日期:2012-12-01 00:00:00
abstract::There is an extensive and still growing body of the literature supporting the efficacy of creatine (Cr) supplementation. In sports, creatine has been recognized as the most effective nutritional supplement in enhancing exercise tolerance, muscle strength and lean body mass. From a clinical perspective, the application...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-011-1132-7
更新日期:2012-08-01 00:00:00
abstract::The daily requirement of a 70-kg male for creatine is about 2 g; up to half of this may be obtained from a typical omnivorous diet, with the remainder being synthesized in the body Creatine is a carninutrient, which means that it is only available to adults via animal foodstuffs, principally skeletal muscle, or via su...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-016-2188-1
更新日期:2016-08-01 00:00:00
abstract::Based on ubiquitous presence of large ionic motifs and clusters in proteins involved in gene transcription and protein synthesis, we analyzed the distribution of ionizable sidechains in a broad selection of proteins with regulatory, metabolic, structural and adhesive functions, in agonist, antagonist, toxin and antimi...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-012-1418-4
更新日期:2012-12-01 00:00:00
abstract::Fibronectin (FN) is a cell adhesion protein that binds integrins in a process also involving the protein-crosslinking enzyme transglutaminase 2 (TG2) as a co-receptor. The cell-adhesive property of TG2 has been linked to a complex formation with FN and to its ability to crosslink and polymerize FN on the cell surface....
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-008-0125-7
更新日期:2009-04-01 00:00:00
abstract::Amino acid networks (AANs) are undirected networks consisting of amino acid residues and their interactions in three-dimensional protein structures. The analysis of AANs provides novel insight into protein science, and several common amino acid network properties have revealed diverse classes of proteins. In this revi...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-014-1710-6
更新日期:2014-06-01 00:00:00
abstract::The synthesis of enantiomerically pure 2-aminocyclobutanecarboxylic acids has been refined to improve both the efficiency and the simplicity. These improvements provide a shorter and easier access to the racemic cis-cyclobutane β-amino acid core. Derivatization of this material with a chiral non-racemic oxazolidin-2-o...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-0918-y
更新日期:2011-08-01 00:00:00
abstract::Mice over-expressing the creatine transporter have elevated myocardial creatine levels [Cr] and are protected against ischaemia/reperfusion injury via improved energy reserve. However, mice with very high [Cr] develop cardiac hypertrophy and dysfunction. To investigate these contrasting effects, we applied a non-biase...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-016-2236-x
更新日期:2016-08-01 00:00:00
abstract::Agmatine, a natural polyamine produced from arginine by arginine decarboxylase, was first discovered in 1910, but its physiological significance was disregarded for a century. The recent rediscovery of agmatine as an endogenous ligand for α2-adrenergic and imidazoline receptors in the mammalian brain suggests that thi...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-019-02720-7
更新日期:2020-02-01 00:00:00
abstract::A thioalkylation procedure, which uses molecular sieves to promote the reaction, was exploited to provide peptides with useful functional groups (lipidic moieties), naturally occurring on proteins as post-translational modifications. The procedure was further implemented to synthesize tailor-made lipidated peptides, i...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1742-y
更新日期:2014-08-01 00:00:00
abstract::In 3T3 cells temperatures higher than physiological stimulated amino acid transport activity in a dose-dependent manner up to 44 degrees C. However, the temperature increase did not induce widespread transport increase of all other nutrients tested. The activities of both amino acid transport systems A and ASC were en...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s007260170033
更新日期:2001-01-01 00:00:00
abstract::Tryptophan is important both for protein synthesis and as a precursor of niacin, serotonin and other metabolites. Tryptophan is an unusual amino acid because of the complexity of its metabolism, the variety and importance of its metabolites, the number and diversity of the diseases it is involved in, and because of it...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806091
更新日期:1996-03-01 00:00:00
abstract::The purpose of this study was to investigate H(e) concentration in plasma from 80 healthy donors in relation to age (6 newborns are also included), sex, daily variation (9, 11 a.m.; 2, 6, 12 p.m.) and a period of 5 subsequent months. A significant correlation (r = 0.63, p < 0.001) was observed between plasma H(e) and ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806188
更新日期:1993-02-01 00:00:00
abstract::Recently, crystalized mouse ketimine reductase/CRYM complexed with NADPH was found to have pyruvate bound in its active site. We demonstrate that the enzyme binds α-keto acids, such as pyruvate, in solution, and catalyzes the formation of N-alkyl-amino acids from alkylamines and α-keto acids (via reduction of imine in...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-015-2044-8
更新日期:2015-11-01 00:00:00
abstract::Taurine can protect against cardiovascular diseases, whereas elevated levels of plasma homocysteine are associated with atherosclerotic and thromboembolic cardiovascular diseases. To illustrate the effects of taurine on hyperhomocysteinemia, we observed the myocardial mitochondria dysfunction in the rats with hyperhom...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-004-0096-2
更新日期:2004-08-01 00:00:00
abstract::Skin is made up of mainly collagen type I and its structure is stabilised by the formation of covalent immature and mature crosslinks. In this study, collagen immature crosslink hydroxylysinonorleucine (HLNL) was isolated from bovine skin in high purity using two sequential purification steps. These consisted of prepa...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02708-3
更新日期:2019-04-01 00:00:00
abstract::In the absence of exogenous Ca(2+) and Mg(2+) and in the presence of EGTA, which favours the release of endogenous Ca(2+), the polyamine spermine is able to stimulate the activity of pyruvate dehydrogenase complex (PDC) of energized rat liver mitochondria (RLM). This stimulation exhibits a gradual concentration-depend...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-008-0099-5
更新日期:2009-03-01 00:00:00
abstract::New bioinorganic complexes of the aspartic acid with the antimony or bismuth triiodide were synthesized by a direct solid-solid reaction at room temperature. The formula of the complex is MI(3)[OOCCH(2)CH(NH(2))CO](2.5) x 2.5H(2)O (M = Sb, Bi). The complex may be a dimer with bridge structure. The crystal structure of...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-005-0210-0
更新日期:2006-07-01 00:00:00
abstract::The nucleotide (ATP-ADP)/nucleoside (adenosine) ratio in the circulation can modulate the processes of vasoconstriction, vasodilatation and platelet aggregation. The main objective of the present study with rat blood serum was to evaluate the possibility of changes in nucleotide hydrolysis by phenylalanine (Phe) and p...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-002-0345-1
更新日期:2003-06-01 00:00:00