Abstract:
:To determine the feasibility of using a nanoparticle immunoassay for clinical therapeutic drug monitoring (TDM) of docetaxel concentrations, a sensitive and simple method of liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established to measure the docetaxel concentration in human plasma and the results of LC-MS/MS and the immunoassay were compared. Docetaxel and paclitaxel (the internal standard, or IS) in human plasma were extracted through protein precipitation, separated on a Diamonsil C18 column (150 mm × 4.6 mm, 5 μm), ionized with positive ions, and detected with LC-MS/MS in multi-reaction monitoring (MRM) mode. Plasma samples from 248 cancer patients were assayed with LC-MS/MS and a nanoparticle immunoassay. Data from the samples were analyzed with the statistical software SPSS and the software MedCalc. Results indicated that the calibration curve of the validated method of LC-MS/MS was linear over the range of 10-2,000 ng/mL, with an lowest limit of quantitation (LLOQ) of 10 ng/mL, and the intra- and inter- day precision and accuracy were both < ± 15%. Comparison of the two methods indicated that results of the LC-MS/MS were closely related to those of the nanoparticle immunoassay, with a correlation coefficient (R) of 0.965 and acceptable 95% confidence intervals (CI) of ‒ 231.7-331.1 ng/mL. Overall, the established method of LC-MC/MS and the nanoparticle immunoassay were both suitable for measurement of the docetaxel concentration in human plasma, and the immunoassay was far more cost-effective and better at clinical TDM of docetaxel in clinical practice.
journal_name
Biosci Trendsjournal_title
Bioscience trendsauthors
Geng C,Li P,Chen X,Yuan G,Guo N,Liu H,Zhang R,Guo Rdoi
10.5582/bst.2017.01029subject
Has Abstractpub_date
2017-05-23 00:00:00pages
202-208issue
2eissn
1881-7815issn
1881-7823journal_volume
11pub_type
杂志文章abstract::Cardiomyocyte nutrient deprivation is a common clinical event that mediates various cardiac ischemic processes and is associated with autophagy activation and cell survival or death. Luteolin-7-O-glucoside (LUTG) was one of the flavonoid glycosides isolated from Dracocephalum tanguticum. Previous research had showed t...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2017.01111
更新日期:2017-11-20 00:00:00
abstract::Complete loss of function in the WRN: RecQ3 DNA/RNA helicase gene causes Werner Syndrome (WS). WS patients with genetic instability manifest an early onset of age-related diseases including diabetes mellitus (DM), osteoporosis, atherosclerosis, and malignancy as well as early death. In 1,420 patients, WS was reported ...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:
更新日期:2008-08-01 00:00:00
abstract::Human herpesvirus 8 (HHV8) is an important opportunistic infection of HIV/AIDS. However, very little is known about antibody seropositivities to HHV8 lytic and latent antigens among HIV-infected patients in China. Therefore, a cross-sectional study was conducted to explore HHV8 serostatus among 316 HIV-infected patien...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2012.v6.3.122
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abstract::In all malignant diseases, several clinical and histopathological tests are established as standard methods for diagnosis. Alternative diagnostic quantitative methods are still lacking or conflicting in cancer of the cervix. Although DNA analysis was one of the earliest applications in flow cytometry and the DNA conte...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:
更新日期:2010-06-01 00:00:00
abstract::Bu-Shen-Ning-Xin decoction (BSNXD), a traditional Chinese medicine, has been used to prevent and treat age-related diseases such as postmenopausal osteoporosis (PMO) for decades. This study sought to investigate the underlying mechanisms of BSNXD in terms of receptor activation of nuclear factor κB ligand (RANKL)-indu...
journal_title:Bioscience trends
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journal_title:Bioscience trends
pub_type: 杂志文章
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journal_title:Bioscience trends
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doi:10.5582/bst.2016.01217
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abstract::Dengue is an acute febrile illness resulting from infection by a flavivirus transmitted by the Aedes mosquito. It is characterized by bleeding manifestations and a plasma leak syndrome. Hepatic involvement in the form of elevation in transaminases is common. However, acute hepatic failure is uncommon. It is not known ...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2011.v5.1.44
更新日期:2011-01-01 00:00:00
abstract::Transplantation with Wharton's jelly derived mesenchymal stem cells (WJ-MSCs) showed great benefits for restoring myocardial function. However, the outcome of WJ-MSCs transplantation was unsuccessful due to multiple factors including oxidative damage. The presence of oxidative stress due to myocardium injury influence...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2019.01191
更新日期:2019-11-13 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:
更新日期:2008-06-01 00:00:00
abstract::Antibiotics are medicines used to prevent and treat bacterial infections. Antibiotic resistance occurs when bacteria change in response to the use of these medicines. Antibiotic resistance is rising to dangerously high levels in all parts of the world, leading to higher medical costs, prolonged hospital stays, and inc...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2016.01034
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abstract::Hepatitis C, caused by the hepatitis C virus (HCV) that attacks the liver and leads to inflammation, is a severe threat to human health. Pegylated interferon α (INF-α) and ribavirin based therapy was once the standard therapy for HCV infection. However, it is suboptimal in efficacy and poorly tolerated in some patient...
journal_title:Bioscience trends
pub_type: 杂志文章,评审
doi:10.5582/bst.2016.01266
更新日期:2017-03-22 00:00:00
abstract::This meta-analysis aimed to review the regeneration rate of future liver remnant (FLR) and perioperative outcomes after associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) and two-stage hepatectomy (TSH). A web search was performed in "MEDLINE", "EMBASE", and "SCIENCE DIRECT" databases ...
journal_title:Bioscience trends
pub_type: 杂志文章,meta分析,收录出版,评审
doi:10.5582/bst.2015.01139
更新日期:2015-10-01 00:00:00
abstract::The effects of anesthesia with sevoflurane and with propofol on glucose utilization in rats were investigated. Sevoflurane significantly impairs glucose utilization whereas propofol does not. Both insulin secretion and sensitivity affect glucose utilization. Propofol is hydrophobic, and anesthesia with this agent is a...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2014.01060
更新日期:2014-06-01 00:00:00
abstract::In patients at risk of hepatocarcinogenesis, tumors are frequently detected with atypical radiological patterns related to hepatocellular carcinoma (HCC) on imaging studies. Despite their high potential for malignancy, whether to resect such lesions immediately is controversial. Based on histological findings, patient...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2017.01110
更新日期:2017-07-24 00:00:00
abstract::This study is an extension of the previous enhancement of dissolution properties of repaglinide using liquisolid compacts. The development and validation of a highperformance liquid chromatography (HPLC) assay for the determination of repaglinide concentration in rabbit plasma for pharmacokinetic studies is described....
journal_title:Bioscience trends
pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00
abstract::We construct and validate a non-invasive clinical scoring model to predict mortality in HIV/TB patients at end stage of AIDS in China. There were 1,007 HIV/TB patients admitted to Beijing Ditan Hospital from August 2009 to January 2018 included in this study, who were randomly assigned to form derivation cohort and va...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2018.01309
更新日期:2019-05-12 00:00:00
abstract::Human papillomavirus (HPV) infections are common and generally harmless, but persistent infections can bring health problems like cancer and genital warts. For the uninfected group, HPV vaccines provide safe and effective protection, but they're type-restricted and expensive. For those infected, so far there have been...
journal_title:Bioscience trends
pub_type: 杂志文章,评审
doi:10.5582/bst.2017.01056
更新日期:2017-07-24 00:00:00
abstract::A research project involving sheets of retinal pigment epithelium constructed from iPS cells derived from patients with age-related maculopathy is one step closer to being approved for clinical trials by the Japanese Government. Now is the time to make therapies using iPS cells clinically available. ...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:
更新日期:2013-06-01 00:00:00
abstract::The dominant-negative inhibition of KCNJ2-encoded inward rectifier potassium channels (Kir2) is currently considered the best approach to biological pacemakers. We hypothesized that inhibition of the inward rectifier potassium current (IK1) in ventricular myocytes by RNA interference (RNAi) would convert ventricular m...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2012.v6.1.26
更新日期:2012-02-01 00:00:00
abstract::Human serum albumin (HSA) is used as an important plasma volume expander in clinical practice. In the present study, HSA was N-terminally PEGylated and a PEGylated HAS (PEG-HSA) carrying one chain of PEG (20 kDa) per HSA molecule was obtained. The purity, secondary structure and hydrodynamic radius of the modified pro...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:
更新日期:2012-04-01 00:00:00
abstract::We retrospectively analyzed newborns with deafness gene mutations and summarized the relationship between genotype and phenotype to provide a basis for genetic counseling. We studied 582 subjects positive for deafness gene mutations that were treated in the otology outpatient department of Beijing Tongren Hospital, Ca...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2017.01070
更新日期:2017-09-12 00:00:00
abstract::The aim of this study was to explore the value of contrast-enhanced ultrasound (CEUS) in differentiating small renal masses. A total of 102 small renal masses (≤ 3 cm) in 99 patients were examined using conventional ultrasound (CUS) and CEUS, and the findings were reviewed and evaluated in comparison to pathology. Sig...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2015.01080
更新日期:2015-08-01 00:00:00
abstract::Spontaneous esophageal perforation (Boerhaave's syndrome) is an uncommon and challenging condition with significant morbidity and mortality. Surgical treatment is indicated in the large majority of cases and different procedures have been described in this respect. We present the results of a mono-institutional evalua...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2016.01009
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abstract::Precise diagnosis is a key measurement for malaria control and elimination, traditional microscopy and rapid diagnostic tests cannot satisfy the requirements especially in the low transmission endemic areas or in the malaria elimination phase. Polymerase chain reaction (PCR) with high sensitivity and specificity can b...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2018.01198
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abstract::Audiological characteristics were investigated in 81 ears of 53 infants with abnormal transient evoked otoacoustic emission (TEOAE) and normal auditory brainstem response (ABR). The relationship between ABR and other hearing testing methods, including 40Hz auditory event-related potential (40Hz-AERP), auditory steady ...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:
更新日期:2008-10-01 00:00:00
abstract::The current study retrospectively investigated variations in audiological phenotypes in children with GJB2 gene mutations. Subjects were 128 infants and young children who were seen as outpatients by Otology at Beijing Tongren Hospital from 2012 to 2018. Of the 128 subjects, 99 had biallelic truncating (T/T) mutations...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2018.01159
更新日期:2018-09-19 00:00:00
abstract::Citrus unshiu peel has been used to prevent and treat various diseases in traditional East-Asian medicine including in Korea. Extracts of C. unshiu peel are known to have various pharmacological effects including antioxidant, anti-inflammatory, and antibacterial properties. Although the possibility of their anti-cance...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2017.01218
更新日期:2017-11-20 00:00:00
abstract::Malignant pericardial effusion is one of the severe complications in advanced lung cancer patients, seriously affecting the patient's cardiopulmonary function and even life. Pericardial drainage and instillation of anti-neoplastic drugs in the pericardial cavity seems to offer the best chance of controlling pericardia...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2014.01073
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2020.03180
更新日期:2020-09-21 00:00:00