Abstract：
:Ascaris lumbricoides is responsible for a highly disseminated helminth parasitic disease, ascariosis, a relevant parasitosis that responds for great financial burden on the public health system of developing countries. In this work, metabolic fingerprinting using high-resolution mass spectrometry (HRMS) was employed to identify marker molecules from A. lumbricoides in different development stages. We have identified nine biomarkers, such as pheromones and steroidal prohormones in early stages, among other molecules in late development stages, making up four molecules for fertilized eggs, four marker molecules for first larvae (L1) and one marker molecule for third larvae (L3). Therefore, our findings indicate that this approach is suitable for biochemical characterization of A. lumbricoides development stages. Moreover, the straightforward analytical method employed, with almost no sample preparation from a complex matrix (feces) using high-resolution mass spectrometry, suggests that it is possible to seek for an easier and faster way to study animal molding processes.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Melo CF,Esteves CZ,de Oliveira RN,Guerreiro TM,de Oliveira DN,Lima EO,Miné JC,Allegretti SM,Catharino RRdoi
10.1007/s00436-016-5183-2subject
Has Abstractpub_date
2016-11-01 00:00:00pages
4107-4114issue
11eissn
0932-0113issn
1432-1955pii
10.1007/s00436-016-5183-2journal_volume
115pub_type
杂志文章abstract：:Water from Lake Anna in Virginia, a lake that is used to cool reactors at a nuclear power plant and for recreational activities, was assessed for the presence of Naegleria fowleri, an ameba that causes primary amebic meningoencephalitis (PAM). This survey was undertaken because it has been reported that thermally enri...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1275-y
更新日期：2009-04-01 00:00:00
abstract：:The parasite fauna of the stone loach (Barbatula barbatula) was investigated monthly from May to December 2001. Four parasite species were recorded: Gyrodactylus spp. (Monogenea), Tylodelphys clavata (Digenea), Proteocephalus sagittus (Cestoda), and Raphidascaris acus (Nematoda). The changes in the seasonal dynamics o...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1261-y
更新日期：2005-01-01 00:00:00
abstract：:Gregarines thrive in the digestive tract of arthropods and may be deleterious to their hosts, especially when present in high densities. The impact of parasites on these invertebrates may affect both the ecosystem equilibrium and human economic activities. However, information available on gregarines in Spain is limit...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5266-0
更新日期：2017-01-01 00:00:00
abstract：:The present study evaluated the distribution and viability of Toxoplasma gondii tissue cysts in the organs and Brazilian commercial cuts of experimentally infected pigs. The pigs were infected with 3 × 103 oocysts of the T. gondii isolate TgCkBr57 (Type BrII). Mouse bioassays were performed on the brain, retina, tongu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06229-6
更新日期：2019-04-01 00:00:00
abstract：:Cryptosporidium spp. and Giardia duodenalis are protozoan parasites that cause diarrhea in humans and animals. Molecular data on Cryptosporidium spp. and G. duodenalis in calves in the Republic of Korea (ROK) is limited; thus, we investigated the prevalence of Cryptosporidium and Giardia in pre-weaned calves, analyzed...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06482-9
更新日期：2019-12-01 00:00:00
abstract：:Several rhoptry proteins (ROPs) have been confirmed to be critical virulence factors of Toxoplasma gondii strains from North America and Europe. The two active kinases ROP17 and ROP18, and pseudokinase ROP5 were thought to be the key determinants of parasites' virulence in laboratory mice. Given the genetic diversity ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5141-z
更新日期：2016-10-01 00:00:00
abstract：:For years, mammals of the order Pilosa have been considered Leishmania reservoirs. But while most studies have focused on sloth species, anteaters have been overlooked, and in many Leishmania endemic countries like Mexico, no studies have been carried out. The aims of this work were to identify the presence of Leishma...
journal_title：Parasitology research
pub_type： 杂志文章,评审
doi：10.1007/s00436-019-06253-6
更新日期：2019-04-01 00:00:00
abstract：:Systematic, continuous mosquito surveillance is considered the most reliable tool to predict the spread and establishment of alien mosquito species such as the Asian tiger mosquito (Aedes albopictus), Japanese bush mosquito (Aedes japonicus), and the transmission risk of mosquito-borne arboviruses to humans. Only sing...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06287-w
更新日期：2019-05-01 00:00:00
abstract：:Few data are available on the molecular characterization of Cryptosporidium spp. in cattle in China. In the present study, a total of 507 fecal specimens from six dairy farms in Heilongjiang Province were examined for Cryptosporidium spp. by light microscopy of concentrates from the formalin-ethyl acetate sedimentatio...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1457-2
更新日期：2009-09-01 00:00:00
abstract：:Blastocystis is a zoonotic protozoan parasite frequently identified in the intestinal tract of humans and a vast variety of animals, worldwide. Here, we assessed the prevalence of Blastocystis and its subtypes in stool samples of raccoons. Stool samples from 30 raccoons were collected. Total DNA was extracted, and the...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06770-9
更新日期：2020-08-01 00:00:00
abstract：:No fully effective treatment has been developed since the discovery of Chagas' disease. Since drug-resistant Trypanosoma cruzi strains are occurring and the current therapy is effective in the acute phase but with various adverse side effects, more studies are needed to characterize the susceptibility of T. cruzi to n...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2275-x
更新日期：2011-08-01 00:00:00
abstract：:Blastocystis sp. is the most common eukaryotic parasite in the intestinal tract of humans. Due to its strong impact in public health, in this study, we determined the frequency of different Blastocystis subtypes in patients in France. We hypothesized on the mode of transmission and tested a possible relationship betwe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1398-9
更新日期：2009-08-01 00:00:00
abstract：:In urban environments, catch basins serve as major developmental and resting sites for anthropophilic and zoophilic mosquitoes. However, the use of this habitat is inconsistent, with abundance of larvae varying significantly across catch basins at a fine spatial scale. During seasonal summer investigations on mosquito...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-4912-x
更新日期：2016-04-01 00:00:00
abstract：:The efficacy of metaphylactic treatment with toltrazuril (Baycox 5% suspension) against natural infections with Eimeria bovis and/or Eimeria zuernii in calves was investigated. The study was conducted with 208 calves on five calf-rearing farms in Germany and the Czech Republic. All participating farms had a notable in...
journal_title：Parasitology research
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1007/s00436-005-1457-9
更新日期：2005-10-01 00:00:00
abstract：:Schistosomiasis caused by different species of schistosome parasites is one of the most debilitating helminthic diseases of humans worldwide. For decades, chemotherapy is the main method of controlling schistosomiasis. However, the fear of drug resistance has motivated the search for alternatives. It has been demonstr...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06224-x
更新日期：2019-03-01 00:00:00
abstract：:Plasmodium berghei XAT is an irradiation-induced, permanent attenuated derivative from high-virulence P. berghei NK65. Monoclonal antibodies against XAT were developed. By immunofluorescence screening, one monoclonal antibody was identified that was reactive with XAT at the schizont stage but not with NK65 nor with an...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00535143
更新日期：1988-01-01 00:00:00
abstract：:Filter-feeding molluscan shellfish can concentrate environmentally derived waterborne pathogens of humans, which can be utilized in the sanitary assessment of water quality. In the present study, oocysts of Cryptosporidium were detected in Bent mussels (Ischadium recurvum) at two Chesapeake Bay sites from which C. par...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050590
更新日期：1999-07-01 00:00:00
abstract：:Ingestion of the larval nematode Angiostrongylus cantonensis can cause the human eosinophilic meningitis known as angiostrongyliasis. Analysis of the extracts and excretory-secretory (ES) products of A. cantonensis larvae and adult stages on gelatin substrate zymography demonstrated the presence of distinct gelatinoly...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1484-6
更新日期：2005-12-01 00:00:00
abstract：:The present work aimed at studying the possible association of HLA antigens with Entamoeba histolytica/E. dispar asymptomatic infection in a Mexican mestizo population. A case-control design was selected for evaluation of the role of genetic markers in parasite infection. For this purpose the HLA-A, HLA-B, and HLA-DR ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050641
更新日期：1999-10-01 00:00:00
abstract：:Efficacy and safety of treatment with imidacloprid 10%+moxidectin 2.5% spot-on (Advocate, Advantage multi; Bayer AG, Leverkusen, Germany) were tested in dogs naturally infested with Sarcoptes scabiei or Otodectes cynotis in a multi-centre, controlled, randomized, blinded field study conducted in France, Germany, Alban...
journal_title：Parasitology research
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1007/s00436-005-1449-9
更新日期：2005-10-01 00:00:00
abstract：:Amphibian populations are declining around the world, and the main reasons are the environmental changes and pathogens. However, there are few studies addressing the interaction and impact of the different pathogens that affect amphibians, such as hemoparasites. These parasites had been described as common in some amp...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-020-06689-1
更新日期：2020-06-01 00:00:00
abstract：:Immunocytochemical studies using antibodies against various peptides were performed on Echinostoma caproni. Only the presence of substance P or a related peptide was clearly demonstrated in this digenetic trematode. In the central nervous system, substance P-immunoreactive neurons were few and constituted two populati...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00930963
更新日期：1989-01-01 00:00:00
abstract：:A total of 585 persons from several occupational groups (farmers, slaughterhouse staff, hunters, veterinarians) exposed to Toxocara infestations and 50 persons of a control group were tested for the presence of specific antibodies to the Toxocara canis antigen using an enzyme-linked immunosorbent assay and a western b...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-1469-5
更新日期：2005-11-01 00:00:00
abstract：:Antimicrobial peptides are increasingly being explored as alternative agents for therapy against the parasitic protozoan Leishmania. Previously, we reported that the synthetic magainin analog, pexiganan, induced apoptosis of surface protease-deficient Leishmania. Here, we report the development of an arginine-rich var...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3847-3
更新日期：2014-05-01 00:00:00
abstract：:A majority of Kudoa spp. infects the somatic muscle of fish establishing cysts. Previously, elevated humoral responses were detected in BALB/c mice immunised with Kudoa sp. pseudocyst extracts and in BALB/c mice orally inoculated with Kudoa sp. pseudocysts, as well as the presence of anti-Kudoa sp. antibodies in human...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1017-1
更新日期：2008-08-01 00:00:00
abstract：:On the basis of information acquired from local health authorities in Evora district of Portugal on cases of visceral leishmaniasis (VL), an epidemiology survey study was conducted. To determine the prevalence of anti-Leishmania antibodies in the local human and canine populations residing in Evora town and 14 adjacen...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00937115
更新日期：1995-01-01 00:00:00
abstract：:Leishmania (L.) tropica is a causative agent of cutaneous and occasionally visceral or viscerotropic leishmaniasis in humans. The dose of parasites influences the course and outcome of disease in some Leishmania species. The effect of parasite dose on L. tropica infection in an experimental model was studied in the cu...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5991-7
更新日期：2018-09-01 00:00:00
abstract：:Dimethyl sulfoxide (DMSO), commonly used for cryoprotection or for the solvation of cytoskeleton-modifying drugs, causes changes in the topology of the plasma membrane of the tegumental brush border in the tapeworm Hymenolepis diminuta; however, relatively long exposures of high concentrations are required. In tapewor...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00936184
更新日期：1992-01-01 00:00:00
abstract：:Understanding the fundamental factors influencing the epidemiology of wildlife disease is essential to determining the impact of disease on individual health and population dynamics. The host-pathogen-environment relationship of the endangered Australian sea lion (Neophoca cinerea) and the parasitic hookworm, Uncinari...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3997-3
更新日期：2014-09-01 00:00:00
abstract：:Serine oligopeptidases of trypanosomatids are emerging as important virulence factors and therapeutic targets in trypanosome infections. A complete open reading frame of oligopeptidase B from Leishmania amazonensis was amplified with polymerase chain reaction with gradient annealing temperatures using primers designed...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0552-5
更新日期：2007-09-01 00:00:00
