Structural characterisation of medically relevant protein assemblies by integrating mass spectrometry with computational modelling.

Abstract:

:Structural mass spectrometry with its various techniques is a powerful tool for the structural elucidation of medically relevant protein assemblies. It delivers information on the composition, stoichiometries, interactions and topologies of these assemblies. Most importantly it can deal with heterogeneous mixtures and assemblies which makes it universal among the conventional structural techniques. In this review we summarise recent advances and challenges in structural mass spectrometric techniques. We describe how the combination of the different mass spectrometry-based methods with computational strategies enable structural models at molecular levels of resolution. These models hold significant potential for helping us in characterizing the function of protein assemblies related to human health and disease. SIGNIFICANCE:In this review we summarise the techniques of structural mass spectrometry often applied when studying protein-ligand complexes. We exemplify these techniques through recent examples from literature that helped in the understanding of medically relevant protein assemblies. We further provide a detailed introduction into various computational approaches that can be integrated with these mass spectrometric techniques. Last but not least we discuss case studies that integrated mass spectrometry and computational modelling approaches and yielded models of medically important protein assembly states such as fibrils and amyloids.

journal_name

J Proteomics

journal_title

Journal of proteomics

authors

Politis A,Schmidt C

doi

10.1016/j.jprot.2017.04.019

subject

Has Abstract

pub_date

2018-03-20 00:00:00

pages

34-41

eissn

1874-3919

issn

1876-7737

pii

S1874-3919(17)30146-X

journal_volume

175

pub_type

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