Abstract:
:Most anticancer drugs are intended to function in the nuclei of cancer cells. If an anticancer drug could be delivered to mitochondria, the source of cellular energy, this organelle would be destroyed, resulting in the arrest of the energy supply and the killing of the cancer cells. To achieve such an innovative strategy, a mitochondrial drug delivery system targeted to cancer cells will be required. We recently reported on the development of a MITO-Porter, a liposome for mitochondrial delivery. In this study, we validated the utility of such a cancer therapeutic strategy by delivering anticancer drugs directly to mitochondria. We succeeded in packaging doxorubicin (DOX) as a model cargo in MITO-Porter to produce a DOX-MITO-Porter. We evaluated cellular toxicity of OS-RC-2 cell, a type of DOX-resistant cancer cell, after delivering DOX to mitochondria using the MITO-Porter system. Cell viability was decreased by the DOX-MITO-Porter treatment, while cell viability was not decreased in the case of naked DOX and a conventional DOX liposomal formulation. We also found a relationship between cellular toxicity and mitochondrial toxicity. The use of a MITO-Porter system for mitochondrial delivery of a toxic agent represents a possible therapeutic strategy for treating drug-resistant cancers.
journal_name
J Pharm Scijournal_title
Journal of pharmaceutical sciencesauthors
Yamada Y,Munechika R,Kawamura E,Sakurai Y,Sato Y,Harashima Hdoi
10.1016/j.xphs.2017.04.058subject
Has Abstractpub_date
2017-09-01 00:00:00pages
2428-2437issue
9eissn
0022-3549issn
1520-6017pii
S0022-3549(17)30327-1journal_volume
106pub_type
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journal_title:Journal of pharmaceutical sciences
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pub_type: 杂志文章,评审
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journal_title:Journal of pharmaceutical sciences
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doi:10.1002/jps.2600740521
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journal_title:Journal of pharmaceutical sciences
pub_type: 指南,杂志文章
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journal_title:Journal of pharmaceutical sciences
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doi:10.1002/jps.2600810808
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journal_title:Journal of pharmaceutical sciences
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journal_title:Journal of pharmaceutical sciences
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abstract::The aim of this work was to evaluate the low molecular weight chitosans (LMWCs) as enhancers of transdermal administration of baicalin, an useful drug for the treatment of atopic dermatitis, viral hepatitis, and HIV infection. Permeation experiments were performed in vitro through mouse skin by using Franz cells. Impr...
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