In vitro determination of the solubility limit of cholesterol in phospholipid bilayers.

Abstract:

:Cholesterol has limited solubility in phospholipid bilayers. The solubility limit is strongly dependent on the nature of the lipid with which the cholesterol is mixed while properties of the crystals formed can be modified by phospholipid-cholesterol interactions. In this review we summarize the various methods that have been developed to prepare hydrated mixtures of cholesterol and phospholipid. We point out some of the factors that determine the form adopted when cholesterol crystallizes in such mixtures, i.e. two- or three-dimensional, monohydrate or anhydrous. These differences can greatly affect the ability to experimentally detect the presence of these crystals in a membrane. Several methods for detecting cholesterol crystals are discussed and compared including DSC, X-ray and GIXRD diffraction methods, NMR and EPR spectroscopy. The importance of the history of the sample in determining the amount and nature of the cholesterol crystals formed is emphasized.

journal_name

Chem Phys Lipids

authors

Epand RM,Bach D,Wachtel E

doi

10.1016/j.chemphyslip.2016.06.006

subject

Has Abstract

pub_date

2016-09-01 00:00:00

pages

3-10

eissn

0009-3084

issn

1873-2941

pii

S0009-3084(16)30065-2

journal_volume

199

pub_type

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