Characterization of specific binding of atrial natriuretic peptide (ANP) to rat PC12 phaeochromocytoma cells.

Abstract:

:Specific binding sites for atrial natriuretic peptide have been identified in membrane of the phaeochromocytoma cell line PC12. Scatchard analysis of binding studies revealed a Kd of 794 pM and a density (Bmax) of 254 fmol/mg protein. Hormones unrelated to ANP such as angiotensin II, bradykinin and arginine-8-vasopressin did not complete for the binding sites. Of the ANP-related peptides which competed for the binding sites, the following order of affinity was established; rANP (8-33) greater than rANP (28 amino acid) greater than rat atrial peptide fragment (13-28) greater than a-hANP (28 amino acid) greater than atrial peptide fragment (1-11) greater than atriopeptin I.

journal_name

Life Sci

journal_title

Life sciences

authors

Boumezrag A,Lyall F,Dow JA

doi

10.1016/0024-3205(88)90577-2

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

2035-42

issue

24

eissn

0024-3205

issn

1879-0631

pii

0024-3205(88)90577-2

journal_volume

43

pub_type

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