Comparative Proteomic Analysis of Mycobacterium tuberculosis Lineage 7 and Lineage 4 Strains Reveals Differentially Abundant Proteins Linked to Slow Growth and Virulence.

Abstract:

:In order to decipher the nature of the slowly growing Mycobacterium tuberculosis (M.tuberculosis) lineage 7, the differentially abundant proteins in strains of M. tuberculosis lineage 7 and lineage 4 were defined. Comparative proteomic analysis by mass spectrometry was employed to identify, quantitate and compare the protein profiles of strains from the two M. tuberculosis lineages. Label-free peptide quantification of whole cells from M. tuberculosis lineage 7 and 4 yielded the identification of 2825 and 2541 proteins, respectively. A combined total of 2867 protein groups covering 71% of the predicted M. tuberculosis proteome were identified. The abundance of 125 proteins in M. tuberculosis lineage 7 and 4 strains was significantly altered. Notably, the analysis showed that a number of M. tuberculosis proteins involved in growth and virulence were less abundant in lineage 7 strains compared to lineage 4. Five ABC transporter proteins, three phosphate binding proteins essential for inorganic phosphate uptake, and six components of the type 7 secretion system ESX-3 involved in iron acquisition were less abundant in M. tuberculosis lineage 7. This proteogenomic analysis provided an insight into the lineage 7-specific protein profile which may provide clues to understanding the differential properties of lineage 7 strains in terms of slow growth, survival fitness, and pathogenesis.

journal_name

Front Microbiol

authors

Yimer SA,Birhanu AG,Kalayou S,Riaz T,Zegeye ED,Beyene GT,Holm-Hansen C,Norheim G,Abebe M,Aseffa A,Tønjum T

doi

10.3389/fmicb.2017.00795

subject

Has Abstract

pub_date

2017-05-09 00:00:00

pages

795

issn

1664-302X

journal_volume

8

pub_type

杂志文章
  • Changes of the Gastric Mucosal Microbiome Associated With Histological Stages of Gastric Carcinogenesis.

    abstract::The changes of gastric microbiome across stages of neoplastic progression remain poorly understood, especially for intraepithelial neoplasia (IN) which has been recognized as a phenotypic bridge between atrophic/intestinal metaplastic lesions and invasive cancer. The gastric microbiota was investigated in 30 healthy c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00997

    authors: Wang Z,Gao X,Zeng R,Wu Q,Sun H,Wu W,Zhang X,Sun G,Yan B,Wu L,Ren R,Guo M,Peng L,Yang Y

    更新日期:2020-05-29 00:00:00

  • Analysis of Two Complementary Single-Gene Deletion Mutant Libraries of Salmonella Typhimurium in Intraperitoneal Infection of BALB/c Mice.

    abstract::Two pools of individual single gene deletion (SGD) mutants of S. Typhimurium 14028s encompassing deletions of 3,923 annotated non-essential ORFs and sRNAs were screened by intraperitoneal (IP) injection in BALB/c mice followed by recovery from spleen and liver 2 days post infection. The relative abundance of each muta...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01455

    authors: Silva-Valenzuela CA,Molina-Quiroz RC,Desai P,Valenzuela C,Porwollik S,Zhao M,Hoffman RM,Andrews-Polymenis H,Contreras I,Santiviago CA,McClelland M

    更新日期:2016-01-05 00:00:00

  • LAM Genes Contribute to Environmental Stress Tolerance but Sensibilize Yeast Cells to Azoles.

    abstract::Lam proteins transport sterols between the membranes of different cellular compartments. In Saccharomyces cerevisiae, the LAM gene family consists of three pairs of paralogs. Because the function of paralogous genes can be redundant, the phenotypes of only a small number of LAM gene deletions have been reported; thus,...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00038

    authors: Sokolov SS,Vorobeva MA,Smirnova AI,Smirnova EA,Trushina NI,Galkina KV,Severin FF,Knorre DA

    更新日期:2020-01-28 00:00:00

  • Potential benefits of the application of yeast starters in table olive processing.

    abstract::Yeasts play an important role in the food and beverage industry, especially in products such as bread, wine, and beer, among many others. However, their use as a starter in table olive processing has not yet been studied in detail. The candidate yeast strains should be able to dominate fermentation, together with lact...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00161

    authors: Arroyo-López FN,Romero-Gil V,Bautista-Gallego J,Rodríguez-Gómez F,Jiménez-Díaz R,García-García P,Querol A,Garrido-Fernández A

    更新日期:2012-04-27 00:00:00

  • Malate-Dependent Carbon Utilization Enhances Central Metabolism and Contributes to Biological Fitness of Laribacter hongkongensis via CRP Regulation.

    abstract::Metabolic adaptation in various environmental niches is crucial for bacterial extracellular survival and intracellular replication during infection. However, the metabolism of carbon/nitrogen sources and related regulatory mechanisms in Laribacter hongkongensis, an asaccharolytic bacterium associated with invasive inf...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01991

    authors: Xiong L,Chan E,Teng JLL,Liu S,Lau SKP,Woo PCY

    更新日期:2019-08-28 00:00:00

  • Oral Treatments With Probiotics and Live Salmonella Vaccine Induce Unique Changes in Gut Neurochemicals and Microbiome in Chickens.

    abstract::Cross-talk between the gut microbiota and neurochemicals affects health and well-being of animals. However, little is known about this interaction in chickens despite their importance in food production. Probiotics and live Salmonella vaccines are microbial products commonly given orally to layer pullets to improve he...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03064

    authors: Redweik GAJ,Daniels K,Severin AJ,Lyte M,Mellata M

    更新日期:2020-01-15 00:00:00

  • Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes.

    abstract::Estuarine sediments are significant repositories of anthropogenic contaminants, and thus knowledge of the impacts of pollution upon microbial communities in these environments is important to understand potential effects on estuaries as a whole. The Lagos lagoon (Nigeria) is one of Africa's largest estuarine ecosystem...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01213

    authors: Obi CC,Adebusoye SA,Ugoji EO,Ilori MO,Amund OO,Hickey WJ

    更新日期:2016-08-05 00:00:00

  • Bacterial Microcompartment-Dependent 1,2-Propanediol Utilization Stimulates Anaerobic Growth of Listeria monocytogenes EGDe.

    abstract::Bacterial microcompartments (BMCs) are proteinaceous organelles that optimize specific metabolic pathways referred to as metabolosomes involving transient production of toxic volatile metabolites such as aldehydes. Previous bioinformatics analysis predicted the presence of BMCs in 23 bacterial phyla including foodborn...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02660

    authors: Zeng Z,Smid EJ,Boeren S,Notebaart RA,Abee T

    更新日期:2019-11-15 00:00:00

  • Soil Photosynthetic Microbial Communities Mediate Aggregate Stability: Influence of Cropping Systems and Herbicide Use in an Agricultural Soil.

    abstract::Edaphic cyanobacteria and algae have been extensively studied in dryland soils because they play key roles in the formation of biological soil crusts and the stabilization of soil surfaces. Yet, in temperate agricultural crop soils, little is understood about the functional significance of indigenous photosynthetic mi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01319

    authors: Crouzet O,Consentino L,Pétraud JP,Marrauld C,Aguer JP,Bureau S,Le Bourvellec C,Touloumet L,Bérard A

    更新日期:2019-06-14 00:00:00

  • Mechanism and In Vivo Evaluation: Photodynamic Antibacterial Chemotherapy of Lysine-Porphyrin Conjugate.

    abstract::Lysine-porphyrin conjugate 4i has potent photosensitive antibacterial effect on clinical isolated bacterial strains such as Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa. The mechanism of photodynamic antibacterial chemotherapy of 4i (4i-PACT) in vitro and the treatme...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00242

    authors: Xu Z,Gao Y,Meng S,Yang B,Pang L,Wang C,Liu T

    更新日期:2016-03-02 00:00:00

  • A Novel Microbial Culture Chamber Co-cultivation System to Study Algal-Bacteria Interactions Using Emiliania huxleyi and Phaeobacter inhibens as Model Organisms.

    abstract::Our understanding of microbial natural environments combines in situ experimentation with studies of specific interactions in laboratory-based setups. The purpose of this work was to develop, build and demonstrate the use of a microbial culture chamber enabling both in situ and laboratory-based studies. The design use...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01705

    authors: Thøgersen MS,Melchiorsen J,Ingham C,Gram L

    更新日期:2018-07-30 00:00:00

  • Biofilm Formation by Pseudallescheria/Scedosporium Species: A Comparative Study.

    abstract::Pseudallescheria/Scedosporium species are medically important fungi that are present in soil and human impacted areas and capable of causing a wide spectrum of diseases in humans. Although little is known about their pathogenesis, their growth process and infection routes are very similar to those of Aspergillus speci...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01568

    authors: Rollin-Pinheiro R,de Meirelles JV,Vila TVM,Fonseca BB,Alves V,Frases S,Rozental S,Barreto-Bergter E

    更新日期:2017-08-18 00:00:00

  • Silver nanoparticles synthesis mediated by new isolates of Bacillus spp., nanoparticle characterization and their activity against Bean Yellow Mosaic Virus and human pathogens.

    abstract::Extracellular agents produced by newly isolated bacterial strains were able to catalyze the synthesis of silver nanoparticles (AgNPs). The most effective isolates were identified as Bacillus pumilus, B. persicus, and Bacillus licheniformis using molecular identification. DLS analysis revealed that the AgNPs synthesize...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00453

    authors: Elbeshehy EK,Elazzazy AM,Aggelis G

    更新日期:2015-05-13 00:00:00

  • Marine-derived fungi: diversity of enzymes and biotechnological applications.

    abstract::The ocean is considered to be a great reservoir of biodiversity. Microbial communities in marine environments are ecologically relevant as intermediaries of energy, and play an important role in nutrient regeneration cycles as decomposers of dead and decaying organic matter. In this sense, marine-derived fungi can be ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00269

    authors: Bonugli-Santos RC,Dos Santos Vasconcelos MR,Passarini MR,Vieira GA,Lopes VC,Mainardi PH,Dos Santos JA,de Azevedo Duarte L,Otero IV,da Silva Yoshida AM,Feitosa VA,Pessoa A Jr,Sette LD

    更新日期:2015-04-10 00:00:00

  • Comprehensive Characterization of the Attenuated Double Auxotroph Mycobacterium tuberculosisΔleuDΔpanCD as an Alternative to H37Rv.

    abstract::Although currently available model organisms such as Mycobacterium smegmatis and Mycobacterium bovis Bacillus Calmette-Guérin (BCG) have significantly contributed to our understanding of tuberculosis (TB) biology, these models have limitations such as differences in genome size, growth rates and virulence. However, at...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01922

    authors: Mouton JM,Heunis T,Dippenaar A,Gallant JL,Kleynhans L,Sampson SL

    更新日期:2019-08-20 00:00:00

  • Bacterial Communities in the Alpaca Gastrointestinal Tract Vary With Diet and Body Site.

    abstract::Gut -associated microbes ('gut microbiota') impact the nutrition of their hosts, especially in ruminants and pseudoruminants that consume high-cellulose diets. Examples include the pseudoruminant alpaca. To better understand how body site and diet influence the alpaca microbiota, we performed three 16S rRNA gene surve...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03334

    authors: Carroll C,Olsen KD,Ricks NJ,Dill-McFarland KA,Suen G,Robinson TF,Chaston JM

    更新日期:2019-01-18 00:00:00

  • The Probiotic Escherichia coli Strain Nissle 1917 Combats Lambdoid Bacteriophages stx and λ.

    abstract::Shiga toxin (Stx) producing E. coli (STEC) such as Enterohemorrhagic E. coli (EHEC) are the major cause of foodborne illness in humans. In vitro studies showed the probiotic Escherichia coli strain Nissle 1917 (EcN) to efficiently inhibit the production of Stx. Life threatening EHEC strains as for example the serotype...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00929

    authors: Bury S,Soundararajan M,Bharti R,von Bünau R,Förstner KU,Oelschlaeger TA

    更新日期:2018-05-29 00:00:00

  • Transcriptome analyses to investigate symbiotic relationships between marine protists.

    abstract::Rhizaria are an important component of oceanic plankton communities worldwide. A number of species harbor eukaryotic microalgal symbionts, which are horizontally acquired in the environment at each generation. Although these photosymbioses are determinant for Rhizaria ability to thrive in oceanic ecosystems, the mecha...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00098

    authors: Balzano S,Corre E,Decelle J,Sierra R,Wincker P,Da Silva C,Poulain J,Pawlowski J,Not F

    更新日期:2015-03-17 00:00:00

  • Fungal metabolic gene clusters-caravans traveling across genomes and environments.

    abstract::Metabolic gene clusters (MGCs), physically co-localized genes participating in the same metabolic pathway, are signature features of fungal genomes. MGCs are most often observed in specialized metabolism, having evolved in individual fungal lineages in response to specific ecological needs, such as the utilization of ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00161

    authors: Wisecaver JH,Rokas A

    更新日期:2015-03-03 00:00:00

  • A miRNA Host Response Signature Accurately Discriminates Acute Respiratory Infection Etiologies.

    abstract::Background: Acute respiratory infections (ARIs) are the leading indication for antibacterial prescriptions despite a viral etiology in the majority of cases. The lack of available diagnostics to discriminate viral and bacterial etiologies contributes to this discordance. Recent efforts have focused on the host respons...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02957

    authors: Poore GD,Ko ER,Valente A,Henao R,Sumner K,Hong C,Burke TW,Nichols M,McClain MT,Huang ES,Ginsburg GS,Woods CW,Tsalik EL

    更新日期:2018-12-11 00:00:00

  • Transformation of Penicillium rubens 212 and Expression of GFP and DsRED Coding Genes for Visualization of Plant-Biocontrol Agent Interaction.

    abstract::Strain 212 of Penicillium rubens (PO212) is an effective fungal biological control agent against a broad spectrum of diseases of horticultural plants. A pyrimidine auxotrophic isolate of PO212, PO212_18.2, carrying an inactive pyrG gene, has been used as host for transformation by positive selection of vectors contain...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01653

    authors: Villarino M,Espeso EA,Melgarejo P,Larena I

    更新日期:2018-07-23 00:00:00

  • Predicting the Responses of Soil Nitrite-Oxidizers to Multi-Factorial Global Change: A Trait-Based Approach.

    abstract::Soil microbial diversity is huge and a few grams of soil contain more bacterial taxa than there are bird species on Earth. This high diversity often makes predicting the responses of soil bacteria to environmental change intractable and restricts our capacity to predict the responses of soil functions to global change...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00628

    authors: Le Roux X,Bouskill NJ,Niboyet A,Barthes L,Dijkstra P,Field CB,Hungate BA,Lerondelle C,Pommier T,Tang J,Terada A,Tourna M,Poly F

    更新日期:2016-05-17 00:00:00

  • Association of antibodies to Plasmodium falciparum reticulocyte binding protein homolog 5 with protection from clinical malaria.

    abstract::Emerging evidence suggests that antibodies against merozoite proteins involved in Plasmodium falciparum invasion into the red blood cell (RBC) play an important role in clinical immunity to malaria. The protein family of parasite antigens known as P. falciparum reticulocyte binding protein-like homolog (PfRh) is requi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00314

    authors: Chiu CY,Healer J,Thompson JK,Chen L,Kaul A,Savergave L,Raghuwanshi A,Li Wai Suen CS,Siba PM,Schofield L,Mueller I,Cowman AF,Hansen DS

    更新日期:2014-06-30 00:00:00

  • Inheritance and Establishment of Gut Microbiota in Chickens.

    abstract::In mammals, the microbiota can be transmitted from the placenta, uterus, and vagina of the mother to the infant. Unlike mammals, development of the avian embryo is a process isolated from the mother and thus in the avian embryo the gut microbial developmental process remains elusive. To explore the establishment and i...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01967

    authors: Ding J,Dai R,Yang L,He C,Xu K,Liu S,Zhao W,Xiao L,Luo L,Zhang Y,Meng H

    更新日期:2017-10-10 00:00:00

  • Automated quantification of the phagocytosis of Aspergillus fumigatus conidia by a novel image analysis algorithm.

    abstract::Studying the pathobiology of the fungus Aspergillus fumigatus has gained a lot of attention in recent years. This is due to the fact that this fungus is a human pathogen that can cause severe diseases, like invasive pulmonary aspergillosis in immunocompromised patients. Because alveolar macrophages belong to the first...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00549

    authors: Kraibooj K,Schoeler H,Svensson CM,Brakhage AA,Figge MT

    更新日期:2015-06-09 00:00:00

  • Population Kinetics and Mechanistic Aspects of Saccharomyces cerevisiae Growth in Relation to Selenium Sulfide Nanoparticle Synthesis.

    abstract::Biosynthesis of nanoparticles (NPs) by microorganisms is a cost- and energy-effective approach. However, how the production of NPs affects the population of producing organism remains as an unresolved question. The present study aimed to evaluate the kinetics of Saccharomyces cerevisiae growth in relation to synthesis...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01019

    authors: Asghari-Paskiabi F,Imani M,Eybpoosh S,Rafii-Tabar H,Razzaghi-Abyaneh M

    更新日期:2020-05-21 00:00:00

  • Characterization of Two Novel Bacteriophages Infecting Multidrug-Resistant (MDR) Acinetobacter baumannii and Evaluation of Their Therapeutic Efficacy in Vivo.

    abstract::Acinetobacter baumannii is emerging as a challenging nosocomial pathogen due to its rapid evolution of antibiotic resistance. We report characterization of two novel bacteriophages, PBAB08 and PBAB25, infecting clinically isolated, multidrug-resistant (MDR) A. baumannii strains. Both phages belonged to Myoviridae of C...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00696

    authors: Cha K,Oh HK,Jang JY,Jo Y,Kim WK,Ha GU,Ko KS,Myung H

    更新日期:2018-04-10 00:00:00

  • Keystone Microbiomes Revealed by 14 Years of Field Restoration of the Degraded Agricultural Soil Under Distinct Vegetation Scenarios.

    abstract::Agricultural intensification accelerates the degradation of cropland, and restoration has been managed by changing its vegetation. However, the keystone microbiome that drives the decomposition of plant-associated organic matter in the restoration is poorly understood. In this study, we established a 14-year field res...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01915

    authors: Zhang Z,Han X,Yan J,Zou W,Wang E,Lu X,Chen X

    更新日期:2020-08-18 00:00:00

  • The 287,403 bp Mitochondrial Genome of Ectomycorrhizal Fungus Tuber calosporum Reveals Intron Expansion, tRNA Loss, and Gene Rearrangement.

    abstract::In the present study, the mitogenome of Tuber calosporum was assembled and analyzed. The mitogenome of T. calosporum comprises 15 conserved protein-coding genes, two rRNA genes, and 14 tRNAs, with a total size of 287,403 bp. Fifty-eight introns with 170 intronic open reading frames were detected in the T. calosporum m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.591453

    authors: Li X,Li L,Bao Z,Tu W,He X,Zhang B,Ye L,Wang X,Li Q

    更新日期:2020-12-09 00:00:00

  • Systematic Identification and Analysis of Acinetobacter baumannii Type VI Secretion System Effector and Immunity Components.

    abstract::Many Gram-negative bacteria use a type VI secretion system (T6SS) for microbial warfare and/or host manipulation. Acinetobacter baumannii is an important nosocomial pathogen and many A. baumannii strains utilize a T6SS to deliver toxic effector proteins to surrounding bacterial cells. These toxic effectors are usually...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02440

    authors: Lewis JM,Deveson Lucas D,Harper M,Boyce JD

    更新日期:2019-10-30 00:00:00