Abstract:
:Periodontal ligament is considered a rich source of mesenchymal stem cells for pulp regeneration. This study investigated the effect of iloprost, a prostacyclin analog, on cell proliferation and the expression of angiogenesis-related genes in human periodontal ligament cells (hPDLs) in vitro. The hPDLs were treated with 10-9-10-6 M iloprost for 1, 6 and 24 h. Cell proliferation was determined by an MTT assay. Vascular endothelial growth factor (VEGF), alpha-1 type I collagen (COL1), and basic fibroblast growth factor (bFGF) mRNA expression were determined by semi-quantitative and qPCR. ELISA was employed for assessment of VEGF expression. Immunofluorescence staining for COL1 protein expression was performed. A prostacyclin receptor (IP) antagonist was used to verify the signaling pathway. The Kruskal-Wallis and Tukey significant difference tests were used to analyze the results. From the results, iloprost treatment did not affect cell morphology or proliferation. Iloprost induced the upregulation of VEGF and COL1 mRNA levels as shown by PCR. The effect of iloprost on bFGF mRNA expression was not observed. The immunofluorescence assay revealed that COL1 protein expression was increased in the iloprost groups. Pretreating the hPDLs with the IP antagonist significantly suppressed the enhancing effect of iloprost on VEGF and COL1 mRNA expression and suppressed COL1 protein expression. In conclusion, iloprost promoted mRNA and protein expression of VEGF and COL1, but not of bFGF in hPDL cells. The increased effect of iloprost was abolished by IP receptor antagonist pretreatment. Iloprost might be a promising agent in dentin-pulp regeneration.
journal_name
Odontologyjournal_title
Odontologyauthors
Jearanaiphaisarn T,Sanharati T,Pavasant P,Nakalekha Limjeerajarus Cdoi
10.1007/s10266-017-0307-4subject
Has Abstractpub_date
2018-01-01 00:00:00pages
11-18issue
1eissn
1618-1247issn
1618-1255pii
10.1007/s10266-017-0307-4journal_volume
106pub_type
杂志文章相关文献
Odontology文献大全abstract::In order to clarify the influence of epinephrine in local anesthetics on endogenous epinephrine, we examined the concentration of plasma catecholamines (epinephrine and norepinephrine) and hemodynamics by administering 4 ml of 2% lidocaine containing different concentrations of epinephrine. Forty-three healthy adult m...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-005-0044-y
更新日期:2005-09-01 00:00:00
abstract::Oral scanners allow dental impressions to be taken in a short time without the use of an impression material. However, it has been noted that high impression accuracy cannot be obtained in cases where the abutment is inclined or in cases where the span is long. Consequently, in daily clinical practice, impressions are...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-020-00537-5
更新日期:2021-01-01 00:00:00
abstract::Apical periodontitis (AP) is an inflammatory disease caused by bacteria infection and is regarded as a common disease in the world. In the progression of AP, the function of nucleotide-binding oligomerization, leucine-rich repeat and pyrin domain domains-containing protein 3 (NLRP3) inflammasome has been revealed. Alt...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-020-00519-7
更新日期:2020-07-01 00:00:00
abstract::There exists a close connection between changes occurring in the teeth and those occurring in the jaw during the evolutionary process. In mammals, the roots of teeth are supported, along with periodontal ligaments and alveolar bones by a unique structure termed the gomphosis. In the present study, we performed combine...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-020-00489-w
更新日期:2020-07-01 00:00:00
abstract::The purpose of this study was to evaluate the effect of filler size on the wear of resin cements. Materials tested included four experimental dual-cure resin cements (Kuraray) consisting of different-sized filler particles. A rectangular box cavity was prepared on the flattened occlusal surface of extracted human mola...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-001-8183-2
更新日期:2001-11-01 00:00:00
abstract::The aim of this study was to compare the immediate postoperative period of participants rehabilitated with dental implants placed with a conventional technique or with a minimally invasive technique, without a mucoperiosteal flap elevation (flapless). Participants who needed implant placement were divided into two gro...
journal_title:Odontology
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10266-018-0343-8
更新日期:2018-07-01 00:00:00
abstract::The aims of this study were to (i) evaluate the bone alterations following maxillary molar extraction and (ii) identify the factors associated with bone alterations using a tomographic analysis. Cone Beam Computer Tomographies (CBCTs) of 17 subjects with 25 maxillary molars were analyzed, before and, in average, 12 mo...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-020-00568-y
更新日期:2020-11-11 00:00:00
abstract::Infective endocarditis is frequently attributed to oral streptococci. Although the pathogenetic mechanisms are not well understood, interaction between streptococci and phagocytes is thought to be important for infective endocarditis. In this study, HL-60 cell-derived monocytes were characterized following interaction...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-011-0044-z
更新日期:2012-07-01 00:00:00
abstract::Bisphosphonate effects on the skeleton have been studied in the context of a broad range of disciplines, including endocrinology, orthopedics, oncology, and dentistry. The emergence of osteonecrosis of the jaw as a rare but serious side effect of bisphosphonate treatment has stimulated interest in understanding how th...
journal_title:Odontology
pub_type: 杂志文章,评审
doi:10.1007/s10266-010-0153-0
更新日期:2011-01-01 00:00:00
abstract::The aim was to evaluate the influence of different irrigation solutions on the push-out bond strength (POBS) of three different sealers (AH Plus, BioRoot RCS, GuttaFlow2). Root canals of 180 single-rooted human teeth were instrumented with F360 up to size 45.04. All canals were irrigated with 5 ml NaOCl 3% and 5 ml ED...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-018-0392-z
更新日期:2019-04-01 00:00:00
abstract::To investigate the effect of benzalkonium chloride (BAC) addition to ethylenediaminetetraacetic acid (EDTA) on transforming growth factor-β1 (TGF-β1) release, as well as attachment and proliferation of dental pulp stem cells (DPSCs) on dentin. A total of standard 268 human dentin disks were prepared and immersed in 1....
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-020-00545-5
更新日期:2020-08-07 00:00:00
abstract::Orthodontic treatment has important correlations with periodontal changes. Various mechanical and biological factors are involved in determining such changes, but anatomical and morphological variables, generally addressed as periodontal biotype, play a key role. In measurement of such modifications, digital and non-c...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-018-0370-5
更新日期:2018-10-01 00:00:00
abstract::Internal connection implants have been attracting attention in recent years leading to their being adopted for many implant systems and an increase in market share over other connections. However, internal connection implants have only been used clinically for a short period of time resulting in few clinical studies i...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-013-0141-2
更新日期:2015-01-01 00:00:00
abstract::The aim of this study was to evaluate the bleaching effectiveness and mineral alterations following the use of experimental bleaching gels that included 6% hydrogen peroxide (HP), titanium dioxide (TiO2) and/or chitosan in comparison with the routinely used 35% HP bleaching gel. Thirty-six maxillary anterior teeth wer...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-020-00526-8
更新日期:2021-01-01 00:00:00
abstract::The Stephan Curve has played a dominant role in caries research over the past several decades. What is so remarkable about the Stephan Curve is the plethora of interactions it illustrates and yet acid production remains the dominant focus. Using sophisticated technology, it is possible to measure pH changes in plaque;...
journal_title:Odontology
pub_type: 杂志文章,评审
doi:10.1007/s10266-012-0092-z
更新日期:2013-01-01 00:00:00
abstract::The aim of this study is to determine the levels of MFG-E8 and interleukin (IL)-1β in saliva and gingival crevicular fluid (GCF) associated with periodontal health and disease. Whole saliva and GCF samples were obtained from systemically healthy participants who were either periodontally healthy (n = 24) or suffered f...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-019-00419-5
更新日期:2019-10-01 00:00:00
abstract::The aim of this paper was to demonstrate the treatment outcomes following immediate functional loading concept of short implants inserted for single tooth replacement in the posterior maxilla. The study was performed on 63 patients who received short (6 mm) implants for single tooth replacement in the posterior maxill...
journal_title:Odontology
pub_type: 临床试验,杂志文章
doi:10.1007/s10266-018-0378-x
更新日期:2019-04-01 00:00:00
abstract::Basic activities of daily living (ADL) is a critical indicator of independency in older adults. The purpose of this study is to evaluate impact of ADL decline on coughing ability, as determined by peak expiratory flow rate (PEFR), and to evaluate impact of ADL decline on the ability of tongue cleaning intervention to ...
journal_title:Odontology
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10266-019-00415-9
更新日期:2019-07-01 00:00:00
abstract::Chemerin is a chemoattractant protein that directs inflammatory cells that express its receptor chemokine receptor-like 1 (ChemR23) towards sites of inflammation. C-C chemokine receptor-like 2 (CCRL2), is the other receptor of chemerin, improves the interaction between chemerin and ChemR23. The aim of this study was t...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-017-0297-2
更新日期:2018-01-01 00:00:00
abstract::To compare the 1-year clinical performance of lithium disilicate and resin composite CAD/CAM onlay restorations. Twenty patients that required two restorations in posterior teeth, with at least one cusp to be covered, received two onlays. One was made with IPS e.max CAD (Ivoclar-Vivadent) and the other with Lava Ultim...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-020-00539-3
更新日期:2021-01-01 00:00:00
abstract::The goal of this short communication is to present finite element analysis comparison of the stress distribution between CAD/CAM full crowns made of Lava Ultimate and of IPS e.max CAD, adhesively luted to natural teeth and to implant abutments with the shape of natural teeth. Six 3D models were prepared using a 3D con...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-016-0276-z
更新日期:2017-04-01 00:00:00
abstract::The aim of the study was to investigate any correlation between tactile sensation and occlusal loading conditions of mandibular premolars and molars, by means of tactile detection threshold (TDT). TDTs of mandibular premolars and molars of twenty adults were determined by calibrated monofilaments. Occlusal force (OF) ...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-011-0007-4
更新日期:2011-07-01 00:00:00
abstract::The aim of this clinical study was to evaluate whether or not an association exists between the presence of enamel craze lines and the prevalence of tooth sensitivity (TS) after in-office bleaching. Subjects that met the inclusion criteria (N = 23) were screened to detect the existence of enamel craze lines. In total,...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-013-0104-7
更新日期:2014-07-01 00:00:00
abstract::Dental stem cells, especially dental follicle cells (DFCs) as precursor cells for the periodontium have interesting prospects for regenerative dentistry. During periodontitis, butyrate as a bacterial metabolite and inflammatory agent is often found in millimolar concentrations in periodontal pockets. This study evalua...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-013-0117-2
更新日期:2014-07-01 00:00:00
abstract::The purpose of this study was to examine, morphologically and histochemically, five types of conditioning effects on cavities prepared with an Er,Cr:YSGG laser and an air-turbine. Cavities were prepared using a Waterlase(®) MD turbo handpiece (W) and an air-turbine (AT) on human extracted molars. The cavity conditioni...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-011-0023-4
更新日期:2012-07-01 00:00:00
abstract::We found few studies on the association between maxillary sinus size and malocclusion in an electronic search using PubMed. The purpose of this study was to investigate maxillary sinus size in different malocclusion groups and the association between maxillary sinus size and dentofacial morphology by the use of latera...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-009-0108-5
更新日期:2010-02-01 00:00:00
abstract::The aim of this study is to evaluate through computed tomography differences in cortical plate thickness of condyle in patients with a different facial vertical skeletal pattern. The final sample of this retrospective study included CBCT exams of 60 adult subjects (mean age 33.2 ± 5.6), selected from the digital archi...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-020-00510-2
更新日期:2020-10-01 00:00:00
abstract::Several growth factors and their receptors, such as epidermal growth factor receptor, have been studied as prognostic biomarkers for many epithelial malignancies. The signal transduction cascade of those receptors includes RAS/RAF/ERK, PI3K/Akt/mTOR, and STAT3 pathways. The aim of this study was to investigate the exp...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-019-00428-4
更新日期:2020-01-01 00:00:00
abstract::The purposes of this study were to establish normative data for mesiodistal tooth crown diameters and arch dimensions in Mongolian adults and to compare them with those of Japanese adults. The study materials comprised dental casts of 100 modern Mongolian and 100 Japanese subjects (50 males, 50 females for each) with ...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-013-0130-5
更新日期:2014-07-01 00:00:00
abstract::We examined the expression of keratin 18 (K18), by immunofluorescence staining, while monitoring morphological changes in the periderm on the lingual epithelium of rats by laser-scanning microscopy of epoxy resin-embedded, semi-ultrathin sections. We also examined differential interference contrast (DIC) images of the...
journal_title:Odontology
pub_type: 杂志文章
doi:10.1007/s10266-005-0059-4
更新日期:2006-09-01 00:00:00