Time-lapse changes of in vivo injured neuronal substructures in the central nervous system after low energy two-photon nanosurgery.

Abstract:

:There is currently very little research regarding the dynamics of the subcellular degenerative events that occur in the central nervous system in response to injury. To date, multi-photon excitation has been primarily used for imaging applications; however, it has been recently used to selectively disrupt neural structures in living animals. However, understanding the complicated processes and the essential underlying molecular pathways involved in these dynamic events is necessary for studying the underlying process that promotes neuronal regeneration. In this study, we introduced a novel method allowing in vivo use of low energy (less than 30 mW) two-photon nanosurgery to selectively disrupt individual dendrites, axons, and dendritic spines in the murine brain and spinal cord to accurately monitor the time-lapse changes in the injured neuronal structures. Individual axons, dendrites, and dendritic spines in the brain and spinal cord were successfully ablated and in vivo imaging revealed the time-lapse alterations in these structures in response to the two-photon nanosurgery induced lesion. The energy (less than 30 mW) used in this study was very low and caused no observable additional damage in the neuronal sub-structures that occur frequently, especially in dendritic spines, with current commonly used methods using high energy levels. In addition, our approach includes the option of monitoring the time-varying dynamics to control the degree of lesion. The method presented here may be used to provide new insight into the growth of axons and dendrites in response to acute injury.

journal_name

Neural Regen Res

authors

Zhao Z,Chen S,Luo Y,Li J,Badea S,Ren C,Wu W

doi

10.4103/1673-5374.206644

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

751-756

issue

5

eissn

1673-5374

issn

1876-7958

pii

NRR-12-751

journal_volume

12

pub_type

杂志文章
  • Inflammation and apoptosis accelerate progression to irreversible atrophy in denervated intrinsic muscles of the hand compared with biceps: proteomic analysis of a rat model of obstetric brachial plexus palsy.

    abstract::In treating patients with obstetric brachial plexus palsy, we noticed that denervated intrinsic muscles of the hand become irreversibly atrophic at a faster than denervated biceps. In a rat model of obstetric brachial plexus palsy, denervated intrinsic musculature of the forepaw entered the irreversible atrophy far ea...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.272619

    authors: Yu XH,Wu JX,Chen L,Gu YD

    更新日期:2020-07-01 00:00:00

  • Axon degeneration: make the Schwann cell great again.

    abstract::Axonal degeneration is a pivotal feature of many neurodegenerative conditions and substantially accounts for neurological morbidity. A widely used experimental model to study the mechanisms of axonal degeneration is Wallerian degeneration (WD), which occurs after acute axonal injury. In the peripheral nervous system (...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.205000

    authors: Wong KM,Babetto E,Beirowski B

    更新日期:2017-04-01 00:00:00

  • Islet amyloid polypeptide & amyloid beta peptide roles in Alzheimer's disease: two triggers, one disease.

    abstract::Alzheimer's disease (AD) is a neurodegenerative disorder that affects millions worldwide. Due to population ageing, the incidence of AD is increasing. AD patients develop cognitive decline and dementia, features for which is known, requiring permanent care. This poses a major socio-economic burden on healthcare system...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.300323

    authors: Ferreira S,Raimundo AF,Menezes R,Martins IC

    更新日期:2021-06-01 00:00:00

  • Emodin prevents hypoxic-ischemic neuronal injury: Involvement of the activin A pathway.

    abstract::Emodin, an extract of dried rhizomes and the root of the Rhizoma Polygoni Cuspidati, can protect neurons from hypoxic-ischemic brain damage. This study aimed to verify the underlying mechanism. After PC12 cells had differentiated into neuron-like cells under the induction of mouse nerve growth factor, cells were subje...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2013.15.002

    authors: Guo H,Shen X,Xu Y,Yuan J,Zhao D,Hu W

    更新日期:2013-05-25 00:00:00

  • Sensory reinnervation of free flaps in reconstruction of the breast and the upper and lower extremities.

    abstract::There is long-standing debate about sensate versus non-sensate free microvascular flaps among microsurgeons. The principle of connecting not only the vascular supply, but also sensitive nerves, in free tissue transfer is attractive. However, increased operating time and partial spontaneous innervation led to the commo...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2012.29.006

    authors: Sinis N,Lamia A,Gudrun H,Schoeller T,Werdin F

    更新日期:2012-10-15 00:00:00

  • Neurological functional evaluation based on accurate motions in big animals with traumatic brain injury.

    abstract::An accurate and effective neurological evaluation is indispensable in the treatment and rehabilitation of traumatic brain injury. However, most of the existing evaluation methods in basic research and clinical practice are not objective or intuitive for assessing the neurological function of big animals, and are also ...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.250578

    authors: Jiang JP,Niu XG,Dai C,Ma K,Xu HY,Cheng SX,Zhang ZW,Duan F,Zhu X,Wang YT,Chen XY,Zhang S

    更新日期:2019-06-01 00:00:00

  • Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro.

    abstract::This study investigated the effects of small interfering RNA (siRNA)-mediated silencing of chemokine receptor 4 (CXCR4) on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXC...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.133172

    authors: Chen X,Zhu Y,Han L,Lu H,Hao X,Dong Q

    更新日期:2014-05-15 00:00:00

  • Research on human glioma stem cells in China.

    abstract::Research on human glioma stem cells began early in the 21st century and since then has become a rapidly growing research field with the number of publications increasing year by year. The research conducted by our diverse group of investigators focused primarily on cell culture techniques, molecular regulation, signal...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.219055

    authors: Zhao YD,Zhang QB,Chen H,Fei XF,Shen YT,Ji XY,Ma JW,Wang AD,Dong J,Lan Q,Huang Q

    更新日期:2017-11-01 00:00:00

  • Toxic tau: structural origins of tau aggregation in Alzheimer's disease.

    abstract::Alzheimer's disease is characterized by the extracellular accumulation of the amyloid β in the form of amyloid plaques and the intracellular deposition of the microtubule-associated protein tau in the form of neurofibrillary tangles. Most of the Alzheimer's drugs targeting amyloid β have been failed in clinical trials...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.274329

    authors: Mamun AA,Uddin MS,Mathew B,Ashraf GM

    更新日期:2020-08-01 00:00:00

  • Mechanisms underlying the promotion of functional recovery by deferoxamine after spinal cord injury in rats.

    abstract::Deferoxamine, a clinically safe drug used for treating iron overload, also repairs spinal cord injury although the mechanism for this action remains unknown. Here, we determined whether deferoxamine was therapeutic in a rat model of spinal cord injury and explored potential mechanisms for this effect. Spinal cord inju...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.208591

    authors: Hao J,Li B,Duan HQ,Zhao CX,Zhang Y,Sun C,Pan B,Liu C,Kong XH,Yao X,Feng SQ

    更新日期:2017-06-01 00:00:00

  • Therapies for children with cerebral palsy: A Web of Science-based literature analysis.

    abstract:OBJECTIVE:To identify global research trends in three therapies for children with cerebral palsy. DATA RETRIEVAL:We performed a bibliometric analysis of studies on therapies for children with cerebral palsy from 2002 to 2011 retrieved from Web of Science. SELECTION CRITERIA: INCLUSION CRITERIA:(a) peer-reviewed publ...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2012.33.009

    authors: Mu Y,Li N,Guan L,Wang C,Shang S,Wang Y

    更新日期:2012-11-25 00:00:00

  • The role of autophagy in Parkinson's disease.

    abstract::Although Parkinson's disease is the most common neurodegenerative movement disorder, the mechanisms of pathogenesis remain poorly understood. Recent findings have shown that deregulation of the autophagy-lysosome pathway is involved in the pathogenesis of Parkinson's disease. This review summarizes the most recent fin...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.3969/j.issn.1673-5374.2012.02.011

    authors: Zhang L,Dong Y,Xu X,Xu Z

    更新日期:2012-01-15 00:00:00

  • Aquaporin-4 gene silencing protects injured neurons after early cerebral infarction.

    abstract::Aquaporin-4 regulates water molecule channels and is important in tissue regulation and water transportation in the brain. Upregulation of aquaporin-4 expression is closely related to cellular edema after early cerebral infarction. Cellular edema and aquaporin-4 expression can be determined by measuring cerebral infar...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.160099

    authors: He ZP,Lu H

    更新日期:2015-07-01 00:00:00

  • Anomalous expression of chloride transporters in the sclerosed hippocampus of mesial temporal lobe epilepsy patients.

    abstract::The Na(+)-K(+)-Cl(-) cotransporter 1 and K(+)-Cl(-) cotransporter 2 regulate the levels of intracellular chloride in hippocampal cells. Impaired chloride transport by these proteins is thought to be involved in the pathophysiological mechanisms of mesial temporal lobe epilepsy. Imbalance in the relative expression of ...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2013.06.010

    authors: Cai X,Yang L,Zhou J,Zhu D,Guo Q,Chen Z,Chen S,Zhou L

    更新日期:2013-02-25 00:00:00

  • The calmodulin-dependent protein kinase II inhibitor KN-93 protects rat cerebral cortical neurons from N-methyl-D-aspartic acid-induced injury.

    abstract::In this study, primary cultured cerebral cortical neurons of Sprague-Dawley neonatal rats were treated with 0.25, 0.5, and 1.0 μM calmodulin-dependent protein kinase II inhibitor KN-93 after 50 μM N-methyl-D-aspartic acid-induced injury. Results showed that, compared with N-methyl-Daspartic acid-induced injury neurons...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2013.02.002

    authors: Liu X,Ma C,Xing R,Zhang W,Tian B,Li X,Li Q,Zhang Y

    更新日期:2013-01-15 00:00:00

  • Neuroprotective effect of ischemic preconditioning in focal cerebral infarction: relationship with upregulation of vascular endothelial growth factor.

    abstract::Neuroprotection by ischemic preconditioning has been confirmed by many studies, but the precise mechanism remains unclear. In the present study, we performed cerebral ischemic preconditioning in rats by simulating a transient ischemic attack twice (each a 20-minute occlusion of the middle cerebral artery) before induc...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.135313

    authors: Liu Y,Zhu S,Wang Y,Hu J,Xu L,Ding L,Liu G

    更新日期:2014-06-01 00:00:00

  • Extracellular vesicles in the diagnosis and treatment of central nervous system diseases.

    abstract::Extracellular vesicles, including exosomes and microvesicles, play a fundamental role in the activity of the nervous system, participating in signal transmission between neurons and providing the interaction of central nervous system with all body systems. In many neurodegenerative diseases, neurons pack toxic substan...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.266908

    authors: Shaimardanova AA,Solovyeva VV,Chulpanova DS,James V,Kitaeva KV,Rizvanov AA

    更新日期:2020-04-01 00:00:00

  • Does diffusion tensor data reflect pathological changes in the spinal cord with chronic injury.

    abstract::Magnetic resonance diffusion tensor imaging has been shown to quantitatively measure the early pathological changes in chronic cervical spondylotic myelopathy. In this study, a novel spongy polyurethane material was implanted in the rat C3-5 epidural space to establish a rat model of chronic cervical spondylotic myelo...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2013.36.003

    authors: Lin E,Long H,Li G,Lei W

    更新日期:2013-12-25 00:00:00

  • Amniotic membrane covering for facial nerve repair.

    abstract::Amniotic membranes have been widely used in ophthalmology and skin injury repair because of their anti-inflammatory properties. In this study, we measured therapeutic efficacy and determined if amniotic membranes could be used for facial nerve repair. The facial nerves of eight rats were dissected and end-to-end anast...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2013.11.002

    authors: Karaman M,Tuncel A,Sheidaei S,Senol MG,Karabulut MH,Deveci I,Karaman N

    更新日期:2013-04-15 00:00:00

  • Relationship of gelatinases-tight junction proteins and blood-brain barrier permeability in the early stage of cerebral ischemia and reperfusion.

    abstract::Gelatinases matrix metalloproteinase-2 and matrix metalloproteinase-9 have been shown to mediate claudin-5 and occludin degradation, and play an important regulatory role in blood-brain barrier permeability. This study established a rat model of 1.5-hour middle cerebral artery occlusion with reperfusion. Protein expre...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2012.31.001

    authors: Xin H,Liang W,Mang J,Lin L,Guo N,Zhang F,Xu Z

    更新日期:2012-11-05 00:00:00

  • Neuronal apoptosis in cerebral ischemia/reperfusion area following electrical stimulation of fastigial nucleus.

    abstract::Previous studies have indicated that electrical stimulation of the cerebellar fastigial nucleus in rats may reduce brain infarct size, increase the expression of Ku70 in cerebral ischemia/reperfusion area, and decrease the number of apoptotic neurons. However, the anti-apoptotic mechanism of Ku70 remains unclear. In t...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.131577

    authors: Liu J,Li J,Yang Y,Wang X,Zhang Z,Zhang L

    更新日期:2014-04-01 00:00:00

  • Strategies for myelin regeneration: lessons learned from development.

    abstract::Myelin regeneration is indispensably important for patients suffering from several central nervous system (CNS) disorders such as multiple sclerosis (MS) and spinal cord injury (SCI), because it is not only essential for restoring neurophysiology, but also protects denuded axons for secondary degeneration. Understandi...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.137586

    authors: Bhatt A,Fan LW,Pang Y

    更新日期:2014-07-15 00:00:00

  • Optical control after transfection of channelrhodopsin-2 recombinant adenovirus in visual cortical cells.

    abstract::Channelrhodopsin-2 ectopically expressed in the retina can recover the response to blue light in genetically blind mice and rats, but is unable to restore visual function due to optic nerve or optic tract lesions. Long Evans rats at postnatal day 1 were used for primary culture of visual cortical cells, and 24 hours l...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2012.16.004

    authors: Yao J,Hou W,Wang H,Liu H,Weng C,Yin Z

    更新日期:2012-06-05 00:00:00

  • Detrimental impact of hyperlipidemia on the peripheral nervous system: A novel target of medical epidemiological and fundamental research study.

    abstract::Recently, epidemiological studies on the etiology of peripheral neuropathies have revealed that hyperlipidemia is a novel risk factor. Plasma lipid levels were confirmed to be associated with the incidence of many peripheral neuropathies including axonal distal polyneuropathy, vision and hearing loss, motor nerve syst...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.3969/j.issn.1673-5374.2012.05.011

    authors: Wu S,Cao X,He R,Xiong K

    更新日期:2012-02-15 00:00:00

  • Electroacupuncture induces acute changes in cerebral cortical miRNA profile, improves cerebral blood flow and alleviates neurological deficits in a rat model of stroke.

    abstract::Electroacupuncture has been shown to improve cerebral blood flow in animal models of stroke. However, it is unclear whether electroacupuncture alters miRNA expression in the cortex. In this study, we examined changes in the cerebral cortical miRNA profile, cerebral blood flow and neurological function induced by elect...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.197135

    authors: Zheng HZ,Jiang W,Zhao XF,Du J,Liu PG,Chang LD,Li WB,Hu HT,Shi XM

    更新日期:2016-12-01 00:00:00

  • Mild hypothermia for treatment of diffuse axonal injury: a quantitative analysis of diffusion tensor imaging.

    abstract::Fractional anisotropy values in diffusion tensor imaging can quantitatively reflect the consistency of nerve fibers after brain damage, where higher values generally indicate less damage to nerve fibers. Therefore, we hypothesized that diffusion tensor imaging could be used to evaluate the effect of mild hypothermia o...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.125348

    authors: Jing G,Yao X,Li Y,Xie Y,Li WX,Liu K,Jing Y,Li B,Lv Y,Ma B

    更新日期:2014-01-15 00:00:00

  • Increased expression of Notch1 in temporal lobe epilepsy: animal models and clinical evidence.

    abstract::Temporal lobe epilepsy is associated with astrogliosis. Notch1 signaling can induce astrogliosis in glioma. However, it remains unknown whether Notch1 signaling is involved in the pathogenesis of epilepsy. This study investigated the presence of Notch1, hairy and enhancer of split-1, and glial fibrillary acidic protei...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.4103/1673-5374.130083

    authors: Liu X,Yang Z,Yin Y,Deng X

    更新日期:2014-03-01 00:00:00

  • Molecular mechanisms of the suppression of axon regeneration by KLF transcription factors.

    abstract::Molecular mechanisms of the Krüppel-like family of transcription factors (KLFs) have been studied more in proliferating cells than in post-mitotic cells such as neurons. We recently found that KLFs regulate intrinsic axon growth ability in central nervous system (CNS) neurons including retinal ganglion cells, and hipp...

    journal_title:Neural regeneration research

    pub_type: 杂志文章,评审

    doi:10.4103/1673-5374.139454

    authors: Apara A,Goldberg JL

    更新日期:2014-08-01 00:00:00

  • Hypoxic preconditioning stimulates angiogenesis in ischemic penumbra after acute cerebral infarction.

    abstract::Previous studies have demonstrated the protective effect of hypoxic preconditioning on acute cerebral infarction, but the mechanisms underlying this protection remain unclear. To investigate the protective mechanisms of hypoxic preconditioning in relation to its effects on angiogenesis, we induced a photochemical mode...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2013.31.002

    authors: Li S,Zhang Y,Shao G,Yang M,Niu J,Lv G,Ji X

    更新日期:2013-11-05 00:00:00

  • Overexpression of estrogen receptor beta alleviates the toxic effects of beta-amyloid protein on PC12 cells via non-hormonal ligands.

    abstract::After binding to the estrogen receptor, estrogen can alleviate the toxic effects of beta-amyloid protein, and thereby exert a therapeutic effect on Alzheimer's disease patients. Estrogen can increase the incidence of breast carcinoma and endometrial cancer in post-menopausal women, so it is not suitable for clinical t...

    journal_title:Neural regeneration research

    pub_type: 杂志文章

    doi:10.3969/j.issn.1673-5374.2012.14.008

    authors: Wang H,Si L,Li X,Deng W,Yang H,Yang Y,Fu Y

    更新日期:2012-05-15 00:00:00