Abstract：
:Cystic echinococcosis is a worldwide zoonosis caused by Echinococcus granulosus. Because the methods of diagnosis and treatment for cystic echinococcosis were limited, it is still necessary to screen target proteins for the development of new anti-hydatidosis vaccine. In this study, the triosephosphate isomerase gene of E. granulosus was in silico cloned. The B cell and T cell epitopes were predicted by bioinformatics methods. The cDNA sequence of EgTIM was composition of 1094 base pairs, with an open reading frame of 753 base pairs. The deduced amino acid sequences were composed of 250 amino acids. Five cross-reactive epitopes, locating on 21aa-35aa, 43aa-57aa, 94aa-107aa, 115-129aa, and 164aa-183aa, could be expected to serve as candidate epitopes in the development of vaccine against E. granulosus. These results could provide bases for gene cloning, recombinant expression, and the designation of anti-hydatidosis vaccine.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Wang F,Ye Bdoi
10.1007/s00436-016-5166-3subject
Has Abstractpub_date
2016-10-01 00:00:00pages
3991-8issue
10eissn
0932-0113issn
1432-1955pii
10.1007/s00436-016-5166-3journal_volume
115pub_type
杂志文章abstract：:The metacercarial cyst walls of Paragonimus westermani, P. miyazakii, P. ohirai, and P. iloktsuenensis were examined using ultrastructural and histochemical techniques. The cyst walls of P. westermani, P. miyazakii, and P. ohirai were found to have five distinct layers, whereas the wall of P. iloktsuenensis had only t...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931563
更新日期：1992-01-01 00:00:00
abstract：:The utility of an enzyme-linked immunosorbent assay to determine the sensitization against the trematode Fasciola hepatica in horses from an endemic area (NW Spain) was assessed. Blood samples were collected from 536 horses and tested against a 2.9-kDa recombinant surface protein (FhrAPS) to estimate the presence of I...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2587-x
更新日期：2012-02-01 00:00:00
abstract：:Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 an...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-014-3908-7
更新日期：2014-07-01 00:00:00
abstract：:Diversity in parasite virulence is one of the factors that contribute to the clinical outcome of malaria infections. The association between the severity of Plasmodium falciparum malaria and the number of distinct parasite populations infecting the host (multiplicity of infection) or polymorphism within any of the spe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3325-3
更新日期：2013-04-01 00:00:00
abstract：:Molecular karyotypes that revealed size polymorphisms between homologous forms of chromosomes 1, 2, 3 or 4 in three strains of the coccidial parasite Eimeria tenella were derived by pulsed field gel electrophoresis (PFGE) using brands of agaroses marketed either specifically for PFGE or for purposes such as protein el...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0775-4
更新日期：2003-03-01 00:00:00
abstract：:Ultrastructural characters of the spermiogenesis and mature spermatozoon of Notopentorchis sp. (Cestoda, Cyclophyllidea, Paruterinidae), a parasite from Apus affinis (Aves, Apodiformes, Apodidae) from Gabon, are described by means of transmission electron microscopy. Cytochemical analysis for detection of glycogen was...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-011-2809-2
更新日期：2012-07-01 00:00:00
abstract：:WD40-repeat proteins have four to eight repeating units flanked by Gly-His (GH) and Trp-Asp (WD) at both termini and folds into a beta-propeller. A polypeptide deduced from a Clonorchis sinensis cDNA clone analyzed to have seven WD40-repeats and predicted to form a beta-propeller (CsWD1). The CsWD1 protein was express...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0721-6
更新日期：2007-12-01 00:00:00
abstract：:The development of Babesia equi was studied in the salivary glands of adult female ticks, Boophilus microplus, using a transmission electron microscope (TEM). Engorged nymphs were obtained from splenectomized foals experimentally infected with B. equi and fed in the adult phase for 5 days on rabbits. Sporogony in B. e...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050359
更新日期：1998-01-01 00:00:00
abstract：:From March to October 2003, a total of 2,518 host-seeking Ixodes ricinus ticks (1,944 nymphs, 264 females, 310 males) were collected by blanket dragging at 45 sites all over the city area of Bonn, western Germany, to be checked for Borrelia burgdorferi infection. The collection sites included 20 private gardens, nine ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-004-1240-3
更新日期：2005-01-01 00:00:00
abstract：:Angiostrongylus vasorum is a metastrongyloid nematode that may cause cardiopulmonary disease, neurological signs and coagulopathies in dogs. The parasite has an indirect life cycle with molluscs as intermediate hosts, in which the infective third larval stage develops. Recently, A. vasorum has been repeatedly reported...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3532-y
更新日期：2013-10-01 00:00:00
abstract：:Sarcocystis sarcocysts are common in many species of domestic and wild animals. Here, we report sarcocystosis in muscles from 91 free range elk (Cervus elaphus) from Pennsylvania, USA, tested by histopathology, transmission electron microscopy (TEM), and DNA sequencing. Sarcocysts were detected in hematoxylin and eosi...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-6024-2
更新日期：2018-10-01 00:00:00
abstract：:Presently, there is a need for increased efforts to develop newer and effective methods to control mosquito vectors as the existing chemical and biological methods are not as effective as in earlier period owing to different technical and operational reasons. The use of nanomaterial products in various sectors of scie...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2934-6
更新日期：2012-09-01 00:00:00
abstract：:Canine trypanosomosisis (CT) is a common disease caused by tsetse- and non-tsetse-transmitted trypanosomes worldwide. The severity of the disease varies from acute, sub-acute to chronic with non-specific clinical signs. Here, we attempt in a cross-sectional study to assess the current situation of CT and the role of d...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5598-4
更新日期：2017-11-01 00:00:00
abstract：:Ticks and tick-borne diseases (TBDs) continue to pose an insidious and ever-present threat to livestock and livelihoods across the globe. Two of the most significant TBDs of cattle in Africa are heartwater and babesioisis, caused by Ehrlichia ruminantium and Babesia bigemina respectively. Both pathogens are endemic in...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-019-06204-1
更新日期：2019-03-01 00:00:00
abstract：:An open randomized controlled study of artemether-lumefantrine (AL) and amodiaquine-sulfalene-pyrimethamine (ASP) for the treatment of uncomplicated Plasmodium falciparum malaria was carried out in 181 children. In 79 children, the hepatomegaly reduction ratios (HRR) and the speed of resolution of hepatomegaly, the he...
journal_title：Parasitology research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00436-006-0293-x
更新日期：2007-02-01 00:00:00
abstract：:Blastocystis sp. is the most common eukaryotic parasite in the intestinal tract of humans. Due to its strong impact in public health, in this study, we determined the frequency of different Blastocystis subtypes in patients in France. We hypothesized on the mode of transmission and tested a possible relationship betwe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1398-9
更新日期：2009-08-01 00:00:00
abstract：:When given orally, quinine or salinomycin cause irreversible damage to the plasmodial developmental stages of Henneguya sp., a gill parasite in the tapir fish Gnathonemus petersii. Naturally infected tapir fish measured 75-169 mm in total length and their total weight ranged over 4.3-11.7 g. The fish bore 7-77 plasmod...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-002-0614-7
更新日期：2002-09-01 00:00:00
abstract：:The first description of an electron microscopic study of Trypanosoma corvi in the vector Ornithomyia avicularia is reported. There is a close association between vector and parasite in the midgut, ileum and rectum of the vector. The midgut distribution of parasites is determined by the peritrophic membrane, which con...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931269
更新日期：1989-01-01 00:00:00
abstract：:A case-control study was designed to investigate the role of different Cryptosporidium spp. in Swedish dairy herds with and without calf diarrhoeal problems. Faecal samples were collected from preweaned calves, young stock and cows. Cryptosporidium oocysts were detected by sodium chloride flotation and epifluorescence...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2020-x
更新日期：2010-11-01 00:00:00
abstract：:A taste-enhancement process for cold-stored, raw shell-stock Crassostrea virginica oysters (i.e., application of table salt to shells) when externally contaminated with human enteropathogens intensified spillage of these enteropathogens to oyster' storage containers (77% compared to 27% for controls) but did not, howe...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-007-0664-y
更新日期：2007-11-01 00:00:00
abstract：:Echinostoma trivolvis adults grown in golden hamsters were treated in vitro with [3H]-tyrosine to label sperm and transplanted to uninfected hamsters alone or with four to six unlabeled adults. After 4-5 days, worms were recovered, processed for autoradiography, and observed for silver grains over their seminal recept...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00932258
更新日期：1993-01-01 00:00:00
abstract：:To contribute to the knowledge of the role of small mammals in the transmission cycle of tegumentary leishmaniasis caused by Leishmania braziliensis, we studied the small mammal community and its temporal and spatial association with phlebotominae, as well as small mammal infection by Leishmania spp. by PCR-RFLP analy...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-017-5714-5
更新日期：2018-02-01 00:00:00
abstract：:Levamisole phosphate, chosen based on its 100 % efficacy demonstrated by a previous fecal egg count reduction test (FECRT), was used as the exclusive anthelmintic treatment in the Embrapa Southeast Livestock sheep flock from 2009 to 2014 in a target selected treatment scheme. In the present study, the effectiveness of...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4844-x
更新日期：2016-03-01 00:00:00
abstract：:The present study reports on the antifilarial activity of a marine sponge Haliclona exigua (phylum Porifera). The crude methanol extract and n-butanol-soluble fraction killed adult Brugia malayi at 31.25-microg/ml concentration (both in motility and 3-(4,5 dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay) ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-009-1555-1
更新日期：2009-10-01 00:00:00
abstract：:In Romania, cystic echinococcosis is endemic and affects, besides humans, various animal species including sheep, cattle, and swine. As yet, no molecular-genetic typing has been carried out to clearly identify the putative strains being transmitted there. Parasite samples (protoscoleces or germinal layers) were collec...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-005-0015-9
更新日期：2006-01-01 00:00:00
abstract：:Spermatogenesis, testes, and vas deferens in the parthenogenetic monozoic tapeworm Atractolytocestus huronensis Anthony, 1958 (Cestoda: Caryophyllidea) from Slovakia, parasitizing the carp Cyprinus carpio L., have been investigated by means of transmission electron microscopy for the first time. The present results sh...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-010-2038-0
更新日期：2011-01-01 00:00:00
abstract：:In recent years, the mitochondrial haplotype diversity of the monogenean ectoparasites Gyrodactylus salaris Malmberg, 1957 on Atlantic salmon and G. thymalli Zitnan, 1960 on grayling has been studied intensively to understand the taxonomy and phylogeography of the two species. According to these studies, neither speci...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-006-0423-5
更新日期：2007-05-01 00:00:00
abstract：:Host susceptibility to parasites is likely to be influenced by intrinsic factors, such as host oxidative status determined by the balance between pro-oxidant production and antioxidant defences. As a result, host oxidative status acts as an environmental factor for parasites and may constrain parasite development. We ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-018-5869-8
更新日期：2018-07-01 00:00:00
abstract：:Distinct sequences of Giardia duodenalis assemblages raised the hypothesis that certain assemblages may contribute to its clinical outcome. However, sequences analysis is time consuming, expensive, and needs many manual operations. Nested PCR targeting intergenic spacer (IGS) region was applied successfully to genotyp...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-016-5347-0
更新日期：2017-02-01 00:00:00
abstract：:We report the ultrastructural alterations induced on epimastigotes by nifurtimox and 5-nitro-2-thienyl-malononitrile (5NO2TM), a novel compound with anti-Trypanosoma cruzi activity. Parasites treated with concentrations of nifurtimox lower than usually employed for this kind of study showed vacuolisation, alterations ...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360100497
更新日期：2002-02-01 00:00:00
