Abstract:
:Nasopharyngeal carcinoma (NPC) has a high incidence and mortality rate, particularly in Southern China. Apogossypolone (ApoG2) is a novel derivative of gossypol with antitumor activity and less toxicity. The human NPC CNE-2 cell line was studied in the in vitro model; whilst 4 week-old male nude mice (BALB/c-nu) were inoculated subcutaneously with CNE-2 cells, and xenograft tumors were studied in the in vivo model. Graded concentrations of ApoG2 were used in treatment studies. In ApoG2-treated and control in vitro and in vivo tumor cells, cell apoptosis, and autophagy were evaluated and quantified using fluorescent and transmission electron microscopy and flow cytometry. Hoechst-33258 fluorescence staining was used to evaluate apoptosis in treated and non-treated cell culture and xenograft NPC cells. Western blotting was performed on lysed tumor cells using primary antibodies to B-cell lymphoma-2 (Bcl-2), beclin-1, and β-actin, and flow cytometry results indicated cell apoptosis rates of 3.90±0.34 and 19.52±1.18% in the control and ApoG2-treated cells, respectively (F=485.294, P<0.001). Western blot analysis showed that ApoG2 significantly decreased expression of the Bcl-2 protein in CNE-2 cells, when compared with control cells (F=68.909, P=0.001) and flow cytometry showed cell autophagy rates of 0.92±3.10% of control cells compared with 28.24±7.35% of ApoG2-treated cells (F=31.035, P=0.003). ApoG2 treatment significantly increased beclin-1 protein expression in CNE-2 cells (F=497.906, P<0.001). ApoG2 treatment inhibited NPC xenograft tumor growth by 65.49% (P<0.05). In conclusion, these results support a role for ApoG2 in inhibiting the growth of human NPC cells by inducing apoptosis and autophagy. Future controlled clinical studies could be planned, to define safety, efficacy and dosing regimens for ApoG2 as a potential treatment for patients with NPC.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Zheng R,Chen K,Zhang Y,Huang J,Shi F,Wu G,Wang Sdoi
10.3892/ol.2017.6176subject
Has Abstractpub_date
2017-07-01 00:00:00pages
751-757issue
1eissn
1792-1074issn
1792-1082pii
OL-0-0-6176journal_volume
14pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::Bladder cancer is one of the most common forms of malignancies involving the urinary system and multiple risk factors have been associated with its etiology. The most common of which include cigarette smoking and various occupational or chemical exposures. It is usually diagnosed in older individuals with an average a...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4355
更新日期:2016-05-01 00:00:00
abstract::The aim of the present study was to investigate the association between the expression of chemokine receptors CCR7 and CXCR4 and vascular endothelial growth factor (VEGF)-C and the lymph node metastasis of pancreatic ductal adenocarcinoma (PDAC). The mRNA transcription levels of CCR7, CXCR4 and VEGF-C were measured in...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1261
更新日期:2013-05-01 00:00:00
abstract::The putative oncogenic role of ATP/GTP binding protein like 2 (AGBL2) in catalyzing α-tubulin detyrosination has recently been characterized in cancer. However, the status of AGBL2 expression in ovarian cancer and its potential clinical and prognostic significance remain unclear. In the present study, immunohistochemi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10829
更新日期:2019-11-01 00:00:00
abstract::The current study presents a case of primary T-cell lymphoma (PTBL), unspecified, in a 27-year-old female. The patient received chemotherapy [cyclophosphamide, epirubicin, vindesine and prednisolone (CHOP) and VP-16 plus CHOP (ECHOP)] and autologous peripheral blood stem cell transplantation, however, relapse occurred...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1676
更新日期:2014-01-01 00:00:00
abstract::Resistance to oxaliplatin (L-OHP) is a major obstacle to successful chemotherapy in colorectal cancer (CRC). In the present study, the ability of gambogic acid (GA) to reverse L-OHP resistance in CRC LoVo cells was investigated. L-OHP-resistant LoVo/L-OHP cells were established by exposing them to increasing concentra...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8916
更新日期:2018-08-01 00:00:00
abstract::Breast cancer is the most common malignant disease occurring in women. Conservative breast cancer surgery followed by radiation therapy is currently the standard treatment for this type of cancer. The majority of metastases occur within the scar, which initiated a series of studies. As a result, clinical trials aimed ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6718
更新日期:2017-10-01 00:00:00
abstract::The incidence of intussusception is low in adults, particularly in the descending colon, due to the anatomical attachment of the descending colon to the retroperitoneum. Signet ring cell histology represents ~1% of colon adenocarcinomas and is associated with young patients and a poor clinical outcome. The present stu...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2805
更新日期:2015-03-01 00:00:00
abstract::Patients with chronic inflammatory bowel disease have an increased risk of colorectal cancer, and the differentiation between neoplastic and inflammatory lesions often poses a clinical dilemma. The aim of the present study was to investigate whether diffusion-weighted (DW) magnetic resonance (MR) enterography with ult...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10441
更新日期:2019-08-01 00:00:00
abstract::Chemotherapy failure remains a challenge when treating patients with acute myeloid leukemia (AML), who often suffer from persistent or relapsed disease. The multidrug resistance (MDR) mediated by efflux transporters of the ATP binding cassette (ABC) superfamily is a major obstacle for successful chemotherapy. The pres...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8095
更新日期:2018-05-01 00:00:00
abstract::Distal-less homeobox 6 antisense RNA 1 (DLX6-AS1) is upregulated in various solid tumors and serves a critical role in the tumorigenesis of cancer. However, to the best of our knowledge, the expression of circulating DLX6-AS1 and its role in the diagnosis of non-small cell lung cancer (NSCLC) have not been previously ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10892
更新日期:2019-11-01 00:00:00
abstract::The present study aims to investigate the radiosensitization effect of the migration and invasion inhibitory protein (MIIP) gene on nasopharyngeal carcinoma (NPC) cells. The MIIP gene was transfected into NPC 5-8F and CNE2 cells. The level of MIIP was analyzed by quantitative reverse transcription-polymerase chain rea...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8524
更新日期:2018-06-01 00:00:00
abstract::Cancer cachexia is a syndrome that impairs the quality of life and overall survival of patients, and thus the effectiveness of anticancer agents. There are no effective therapies for cancer cachexia due to the complexity of the syndrome, and insufficient knowledge of its pathogenesis results in difficulty establishing...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7200
更新日期:2017-12-01 00:00:00
abstract::Dendrobium candidum Wall ex Lindl. (D. candidum) is a traditional Chinese medicine widely used in Asia. The present study has showed that D. candidum exerted an anti-metastatic effect in mice injected with 26-M3.1 colon carcinoma cells. D. candidum showed the most marked tumor inhibitory rate of 64.5% at a dose of 400...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2383
更新日期:2014-10-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) can result from hepatitis B or C infection, fibrosis or cirrhosis. Transforming growth factor-β (TGF-β) is one of the main growth factors associated with fibrosis or cirrhosis progression in the liver, but its role is controversial in hepatocarcinogenesis. In the present study, the effec...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8441
更新日期:2018-06-01 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC), one of the most common malignant tumors, endangers human health. Recently, the incidence of HNSCC has kept increasing: However, its prognosis has not significantly improved. Understanding the molecular mechanism underlying HNSCC development will therefore provide new stra...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10920
更新日期:2019-12-01 00:00:00
abstract::Xeroderma pigmentosum variant (XP-V) is a subtype of xeroderma pigmentosum (XP) disease with typical pigmentation and types of cancer in the oral maxillofacial and other sun-exposed regions. Few factors of tumor proneness in XP-V have been completely elucidated with the exception of the POLH [which encodes DNA polymer...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1604
更新日期:2013-12-01 00:00:00
abstract::Previous studies have demonstrated that nicotinamide N-methyltransferase (NNMT) is aberrantly expressed in a number of tumors. In the present study, it was demonstrated that the gene and protein levels of NNMT were significantly increased in gastric cancer cells. Furthermore, upregulation of NNMT significantly increas...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.7885
更新日期:2018-04-01 00:00:00
abstract::Uterine fibroids are the most common type of benign, gynecologic neoplasm and are the primary indication for performance of a hysterectomy, accounting for >200,000 hysterectomies annually in the USA. At present, females are younger and exhibit larger leiomyomas at the time of diagnosis. Cancer-associated fibroblasts i...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2225
更新日期:2014-08-01 00:00:00
abstract::Multifocal epithelial hyperplasia (MEH), also known as Heck's disease, manifests as a papulonodular lesion in the oral mucosa and has been associated with the human papillomavirus, a virus related to various precancerous diseases in the oral cavity. It has a predisposition for the female gender and for children. Altho...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.465
更新日期:2012-02-01 00:00:00
abstract::Trefoil factor 2 (TFF2) plays a protective role in gastric mucosa and may be involved in the progression of gastric cancer, but the detailed functions and underlying molecular mechanisms are not clear. The present study used a combination of clinical observations and molecular methods to investigate the correlation be...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.1904
更新日期:2014-05-01 00:00:00
abstract::PX-12, as an inhibitor of thioredoxin (Trx), has antitumor activity. However, little is known about the toxicological effect of PX-12 on cervical cancer cells. In the present study, the growth inhibitory effects of PX-12 on HeLa cervical cancer cells in association with reactive oxygen species (ROS) and glutathione (G...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1637
更新日期:2013-12-01 00:00:00
abstract::Some colorectal cancer liver metastases (CLMs) disappear on serial imaging during chemotherapy and the optimal treatment strategy for such lesions remains undetermined. The purpose of this study was to investigate the outcome in disappearing CLMs, as few studies have focused on this topic, with conflicting results. Am...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.842
更新日期:2012-11-01 00:00:00
abstract::Gastric cancer (GC) is one of the most common malignancies and is the second leading cause of cancer-associated mortality world-wide. In the present study, the prognostic value and antitumor effects of transforming growth factor β1 (TGFβ1) and its receptors in GC were explored. The online Kaplan-Meier plotter database...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11849
更新日期:2020-09-01 00:00:00
abstract::The aim of the present study was to investigate the effect of HPV16E6 gene integration on the biological behavior of Eca109 and Eca9706 cells. This was evaluated through positive liposome transfection of HPV16E6 into Eca109 cells and Eca9706 cells. The transfection efficiency was evaluated by calculating the ratio of ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7469
更新日期:2018-02-01 00:00:00
abstract::Carbon dioxide (CO2) laser ablation in combination with photodynamic therapy (PDT) has previously been successfully used to treat superficial basal cell carcinoma (BCC). However, the efficacy of this treatment modality is limited in the treatment of deeper lesions and the more aggressive subtypes of BCC. In order to i...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1504
更新日期:2013-10-01 00:00:00
abstract::Gelatinases are overexpressed in several types of maligancies and tumor stromal cells. Lidamycin is an enediyne antitumor antibiotic, which is composed of an apoprotein (LDP) and an active chromophore (AE). It is known that the heavy-chain complementarity-determining region-3 (CDR3) domain of scFv is important in anti...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1143
更新日期:2013-04-01 00:00:00
abstract::Recommendations concerning the intake of vitamin D and/or sunlight exposure in the handling of patients with vitamin D deficiency remain a matter of debate. The present study of the German network of dermato-oncologists (Onkoderm e.V.) refers to an inquiry conducted among general practitioners on this and related issu...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.939
更新日期:2012-12-01 00:00:00
abstract::Despite advancements in treatment regimens, the mortality rate of patients with oral tongue squamous cell carcinoma (OTSCC) is high. In addition, the signaling pathways and oncoproteins involved in OTSCC progression remain largely unknown. Therefore, the aim of the present study was to identify specific prognostic mar...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10982
更新日期:2019-12-01 00:00:00
abstract::The detection of epidermal growth factor receptor (EGFR) mutations is necessary for the selection of suitable patients with non-small cell lung cancer (NSCLC) for treatment with EGFR tyrosine kinase inhibitors. Cytology specimens are known to be suitable for EGFR mutation detection, although tissue specimens should be...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7464
更新日期:2018-02-01 00:00:00
abstract::DNA topoisomerase II-α (Topo II-α) is essential for numerous cell processes, including DNA replication, transcription, recombination, and chromosome separation and condensation. Altered Topo II-α expression may lead to carcinogenesis and cancer progression. The aim of the present study was to investigate the associati...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2367
更新日期:2014-10-01 00:00:00