Abstract：
:Lack of data on safety of herbal medicines have endangered human health and life. The present study evaluated the genotoxic and mutagenic effect of Kalanchoe laciniata to access the safety and usefulness of the medicinal plant. Aqua-methanolic and n-hexane extracts of K. laciniata were evaluated for the genotoxic potential using Ames assay and cytotoxicity was evaluated using MTT assay. Ames assay was conducted using two strains of Salmonella typhimurium TA-100 and TA-102 whereas MTT assay was performed on baby hamster kidney cell line BHK-21. Aqua-methanolic extract of K.laciniata exhibited significant mutagenicity when exposed to TA-102 strain with a mutagenic index of 50.66 and 54.74 at maximum dose 150 mg/plate. The extract was also mutagenic to TA-100 strain but to a lesser extent. M.I of n-hexane extract was 12.15 and 15.51 for TA-100 and TA-102 respectively. n-hexane extract was mutagenic but little difference was observed between results of two strains. Both extracts were found to be cytotoxic with an IC50 of 321.9 and 638.5 µg/mL for aqua-methanolic and n-hexane extracts respectively. On the basis of results it was concluded that aqua-methanolic and n-hexane extracts of K.laciniata possess mutagenic and cytotoxic potential. It is suggested to explore the plant further to evaluate its safety in rodents and other species.
journal_name
EXCLI Jjournal_title
EXCLI journalauthors
Sharif A,Akhtar MF,Akhtar B,Saleem A,Manan M,Shabbir M,Ashraf M,Peerzada S,Ahmed S,Raza Mdoi
10.17179/excli2016-748subject
Has Abstractpub_date
2017-04-24 00:00:00pages
593-601issn
1611-2156pii
Doc593journal_volume
16pub_type
杂志文章相关文献
EXCLI Journal文献大全abstract：:The aim of this study was the design and evaluation of a novel plasma stable, pH-sensitive niosomal formulation of Mitoxantrone by a modified ethanol injection method. Cholesterol hemisuccinate was added instead of cholesterol in order to produce pH-sensitivity property and using PEG-Poly (monomethyl itaconate)-CholC6...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：10.17179/excli2013-609
更新日期：2015-01-06 00:00:00
abstract：:Recently, bioinformatics has advanced to the level that it allows almost accurate prediction of molecular interactions that hold together a protein and a ligand in the bound state. For instance, the program AutoDock has been developed to provide a procedure for predicting the interaction of small molecules with macrom...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：
更新日期：2013-09-23 00:00:00
abstract：:R273H mutant p53 is a DNA-contact mutant that renders p53 dysfunctional due to a single substitution of Arg273 for His273. Rescuing R273 mutant p53 implies that a competent molecule would have to bind to the site of DNA-contact hot spots to complement the loss of contact with the DNA-binding domain. Here, curcumin, fl...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：10.17179/excli2017-299
更新日期：2017-12-08 00:00:00
abstract：:Experimentally-induced total sleep deprivation (TSD) and chronic partial sleep restriction (CPSR) leads to the emergence of cognitive impairments. This is hypothesized to result from a consequent neuroinflammation which may also hasten the neurodegenerative processes. Neuroinflammatory markers such as tumor necrosis f...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：
更新日期：2013-04-14 00:00:00
abstract：:[This retracts the article DOI: 10.17179/excli2015-642.]. ...
journal_title：EXCLI journal
pub_type： 杂志文章,撤回出版物
doi：10.17179/excli2016-570
更新日期：2016-09-19 00:00:00
abstract：:Salmonella enterica serovar Typhi (S. Typhi) is a human restricted pathogen. It biosynthesizes a virulence capsular polysaccharide named as Vi antigen. S. Typhi regulates expression of genes involved in the biosynthesis of Vi antigen in response to osmolarity. Beside Vi-positive isolates, Vi-negative (acapsulated) iso...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：10.17179/excli2014-674
更新日期：2015-02-09 00:00:00
abstract：:Nanoencapsulation of drug/small molecules in nanocarriers (NCs) is a very promising approach for development of nanomedicine. Modern drug encapsulation methods allow efficient loading of drug molecules inside the NCs thereby reducing systemic toxicity associated with drugs. Targeting of NCs can enhance the accumulatio...
journal_title：EXCLI journal
pub_type： 杂志文章,评审
doi：
更新日期：2014-03-20 00:00:00
abstract：:The traditional uses of Portulaca oleracea L. (PO) with anti-inflammatory and anti-cancer activity as well as antioxidants properties were expressed previously. Glioma is considered the most common primary brain tumor and its malignant form is the most lethal adult brain tumor, that glioblastoma covers about 50 % of g...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：10.17179/excli2019-1063
更新日期：2019-03-20 00:00:00
abstract：:Metabolic syndrome (MS) is a condition associated with metabolic abnormalities that are characterized by central obesity (e.g. waist circumference or body mass index), hypertension (e.g. systolic or diastolic blood pressure), hyperglycemia (e.g. fasting plasma glucose) and dyslipidemia (e.g. triglyceride and high-dens...
journal_title：EXCLI journal
pub_type： 杂志文章,评审
doi：10.17179/excli2017-911
更新日期：2018-01-10 00:00:00
abstract：:DNA and chromosomal damage in peripheral blood leukocytes of patients with coronary artery disease (CAD) were investigated by using the single cell gel electrophoresis assay /comet and cytokinesis- block micronucleus (CBMN) assays, respectively. The case-control study comprised patients with CAD (n = 46; average age 5...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：
更新日期：2013-10-08 00:00:00
abstract：:Healthcare professionals, especially nurses and pre-hospital emergency (PHE) staff, are influenced by many stressors due to their responsibility to provide comfort as well as care and treatment of patients. The aim of the present study was to identify and rank the occupational stressors in nurses and PHE staff using F...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：10.17179/excli2018-1505
更新日期：2018-08-20 00:00:00
abstract：:Objective: Although osteoarthritis (OA) is widely accepted as a degenerative disease, autoimmune processes are believed to be involved in the pathogenesis. There are limited studies in this area and most of them focused on antibodies against chondrocyte membrane. In an attempt to address the paucity of evidence in thi...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：
更新日期：2012-09-07 00:00:00
abstract：:There are abundant studies indicating that blocking gap junctions (GJs) containing connexin 36 (Cx36) inhibit seizures. However, recent evidences demonstrate proconvulsant effect of such intervention. Electrical coupling between GABAergic interneurons in CA1 region of hippocampus is mediated through Cx36 GJs. We inves...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：
更新日期：2013-03-14 00:00:00
abstract：:Genome editing technologies include techniques used for desired genetic modifications and allow the insertion, modification or deletion of specific DNA fragments. Recent advances in genome biology offer unprecedented promise for interdisciplinary collaboration and applications in gene editing. New genome editing techn...
journal_title：EXCLI journal
pub_type： 杂志文章,评审
doi：10.17179/excli2020-3070
更新日期：2021-01-04 00:00:00
abstract：:The aim of this study was to evaluate the effect of acute and chronic physical and psychological stressors on the induction of oxidative stress in male rat liver. Male Wistar rats were randomly divided into 3 groups as following: control, physical and psychological stress groups. Stress was induced by communication bo...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：
更新日期：2014-02-24 00:00:00
abstract：:Five Calligonum species endemic to the Tarim Basin - C. roborowskii, C. kuerlese, C. juoqiangense,C. yengisaricum, and C. taklimakanense - were compared using 14 morphological characteristics and nrDNA ITS and cpDNA trnL-F molecular data. Intraspecific and interspecific morphological variation was uncovered, with vari...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：
更新日期：2012-12-20 00:00:00
abstract：:DNA methylation, an epigenetic modification, is mediated by DNA methyltransferases (DNMTs), a family of enzymes. Inhibitions of these enzymes are considered a promising strategy for the treatment of several diseases. In this study, a quantitative structure-activity relationship (QSAR) modeling was employed to understa...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：10.17179/excli2020-1096
更新日期：2020-04-02 00:00:00
abstract：:Dynamin 2 is a GTPase protein that has been implicated in cancer progression through its various roles such as endocytosis, morphogenesis, epithelial-mesenchymal transition (EMT), cellular contractions, and focal adhesion maturation. The increased expression levels of this molecule have been demonstrated with the deve...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：10.17179/excli2020-2762
更新日期：2020-10-29 00:00:00
abstract：:This study was undertaken to determine whether exposure of operating room personnel to inhalation anesthetics, including nitrous oxide, isoflurane, and sevoflurane was associated with any hepatotoxic or nephrotoxic changes. Fifty-two operating room personnel and 52 non-exposed subjects were studied. A questionnaire pe...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：10.17179/excli2019-1911
更新日期：2020-03-25 00:00:00
abstract：:Hepatitis C is a major global health burden and Egypt has the highest prevalence of hepatitis C virus (HCV) worldwide. The current study was designed to evaluate the beneficial therapeutic effects of ethanolic extracts of Nigella sativa, Zingiber officinale and their mixture in Egyptian HCV patients. Sixty volunteer p...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：
更新日期：2013-11-11 00:00:00
abstract：:The aim of this study is to investigate the mechanism of electro-acupuncture (EA) on the recovery of injured spinal cord. Rats were randomly divided into normal control, sham-operated, SCI, SCI+EA group and T10 segment spinal cord injury (SCI) rat model was established by the modified Allen's method. After 7 days, the...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：10.17179/excli2017-123
更新日期：2017-03-23 00:00:00
abstract：:We present the case of a 77 year old male patient with metastatic pancreatic neuroendocrine tumor (NET). The patient was initially treated by extensive surgical resection that however, led to severe complications with delayed recovery. During follow-up, a number of new liver metastases were detected, one of which was ...
journal_title：EXCLI journal
pub_type：
doi：10.17179/excli2019-1220
更新日期：2019-05-21 00:00:00
abstract：:Dibutyltin dichloride (DBTC) is an organotin compound used as model for acute and chronic pancreatitis. Oxidative stress is one of the mechanisms of propagation of acinar cell injury in acute pancreatitis. Selenium is an essential cofactor in the antioxidant glutathione peroxidase pathway. Selenium levels are describe...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：10.17179/excli2016-466
更新日期：2017-02-16 00:00:00
abstract：:Ursolic acid (UA) is a pentacyclic triterpenoid carboxylic acid that is found in plants and herbal products. It is one of the chemopreventive agents, which can suppress cancer cell proliferation and induce apoptosis. UA possesses various biological activities including anticancer, anti-inflammatory, anti-oxidative and...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：
更新日期：2013-02-08 00:00:00
abstract：:The effect of leaf essential oil of Hoslundia opposita (Vahl) on the haematological parameters of alloxan-induced diabetic rats was investigated. Forty-eight albino rats (Rattus norvegicus), of average weight 132.5 g, were randomly selected into normal and diabetic groups, each with four sub-groups. The rats were trea...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：
更新日期：2012-09-25 00:00:00
abstract：:Prosopis cineraria (L.) Druce (Leguminosae) (syn. Prosopis spicigera L.) has antidiabetic and antioxidant potential. Earlier we reported its hypolipidemic activity obtained from ethanol extract (ET-PCF). Object of this work was to isolate ferulic acid (FA) from ET-PCF and evaluate hypolipidemic activity against high-f...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：10.17179/excli2016-394
更新日期：2016-10-25 00:00:00
abstract：:Oxidative stress is the common underlying mechanism of damage in ischemic stroke. Therefore, we aimed to evaluate the possible protective effects of polyhydroxylated fullerene derivatives on brain infarction and oxidative/nitrosative stress in a rat model of ischemic stroke. The experiment was performed by four groups...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：10.17179/excli2016-309
更新日期：2016-06-20 00:00:00
abstract：:Unlike conventional materials that covalent bonds connecting atoms as the major force to hold the materials together, supramolecular biomaterials rely on noncovalent intermolecular interactions to assemble. The reversibility and biocompatibility of supramolecular biomaterials render them with diverse range of function...
journal_title：EXCLI journal
pub_type： 杂志文章,评审
doi：10.17179/excli2020-2656
更新日期：2020-08-05 00:00:00
abstract：:Previous studies proved the pro-angiogenic effect of Crocetin, a natural carotenoid dicarboxylic acid, in both in vivo and in vitro models. However, the exact mechanism of Crocetin action has not completely been elucidated yet. The current experiment was designed to find the activity of PI3K-Akt-eNOS axis after the tr...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：10.17179/excli2019-1175
更新日期：2019-10-21 00:00:00
abstract：:Patients with diabetes tend to have an increased risk of osteoporosis that may be related to hyperglycemia. In vitro evidence has shown that high glucose can affect the proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs). Tissue regeneration depends mainly on MSCs. However, the exact mechanis...
journal_title：EXCLI journal
pub_type： 杂志文章
doi：
更新日期：2013-06-27 00:00:00
