Abstract:
:Pseudomonas stutzeri 273 is a marine bacterium producing exopolysaccharide 273 (EPS273) with high anti-biofilm activity against P. aeruginosa PAO1. Here, the complete genome of P.stutzeri 273 was sequenced and the genome contained a circular 5.03 Mb chromosome. With extensive analysis of the genome, a genetic locus containing 18 genes was predicted to be involved in the biosynthesis of EPS273. In order to confirm this prediction, two adjacent genes (eps273-H and eps273-I) encoding glycosyltransferases and one gene (eps273-O) encoding tyrosine protein kinase within the genetic locus were deleted and biosynthesis of EPS273 was checked in parallel. The molecular weight profile of EPS purified from the mutant Δeps273-HI was obviously different from that purified from wild-type P.stutzeri 273, while the corresponding EPS was hardly detected from the mutant Δeps273-O, which indicated the involvement of the proposed 18-gene cluster in the biosynthesis of EPS273. Moreover, the mutant Δeps273-HI had the biofilm formed earlier compared with the wild type, and the mutant Δeps273-O almost completely lost the ability of biofilm formation. Therefore, EPS273 might facilitate the biofilm formation for its producing strain P.stutzeri 273 while inhibiting the biofilm formation of P. aeruginosa PAO1. This study can contribute to better understanding of the biosynthesis of EPS273 and disclose the biological function of EPS273 for its producing strain P.stutzeri 273.
journal_name
Mar Drugsjournal_title
Marine drugsauthors
Wu S,Zheng R,Sha Z,Sun Cdoi
10.3390/md15070218subject
Has Abstractpub_date
2017-07-10 00:00:00issue
7issn
1660-3397pii
md15070218journal_volume
15pub_type
杂志文章相关文献
Marine Drugs文献大全abstract::Monostroma nitidum is a green single-cell layered algae that grows on the southwest coast of Japan. It is often used for salad ingredients, boiled tsukudani, soups, etc., due to its health benefits. M. nitidum is composed of many cell aggregates, and the various substances that fill the intercellular space are dietary...
journal_title:Marine drugs
pub_type: 杂志文章,评审
doi:10.3390/md18040228
更新日期:2020-04-24 00:00:00
abstract::The total syntheses of the marine-derived lipopeptide natural product fellutamide B and deoxy-fellutamides B, C, and D are reported. These compounds were accessed through a novel solid-phase synthetic strategy using Weinreb amide-derived resin. As part of the synthesis, a new enantioselective route to (3R)-hydroxy lau...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md11072382
更新日期:2013-07-08 00:00:00
abstract::Mouse bioassay is the official testing method to quantify paralytic shellfish toxins (PSTs) in bivalves. A number of alternative analytical methods have been reported. Some methods have been evaluated by a single laboratory validation. Among the different types of methods, chemical analyses are capable of identifying ...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md9030466
更新日期:2011-03-22 00:00:00
abstract::Mammalian target of rapamycin (mTOR) is a PI3K-related serine/threonine protein kinase that functions as a master regulator of cellular growth and metabolism, in response to nutrient and hormonal stimuli. mTOR functions in two distinct complexes-mTORC1 is sensitive to rapamycin, while, mTORC2 is insensitive to this dr...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md16100385
更新日期:2018-10-15 00:00:00
abstract::Three new polyoxygenated steroids, michosterols A-C (1-3), and four known compounds (4-7) were isolated from the ethyl acetate (EtOAc) extract of the soft coral Lobophytum michaelae, collected off the coast of Taitung. The structures of the new compounds were elucidated on the basis of spectroscopic analyses and compa...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md16030093
更新日期:2018-03-13 00:00:00
abstract::Three new aryl alkaloids named suberitamides A-C (1-3), were isolated from an extract of the marine sponge Pseudosuberites sp. collected along the coast of North Carolina. Their planar structures were established by extensive nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis. To assign the challengi...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md18110536
更新日期:2020-10-28 00:00:00
abstract::Tetracenomycin X (Tcm X) has been reported to have antitumour activity in various cancers, but there have not been any studies on its activity with respect to lung cancer to date. Therefore, this study aims to investigate the anti-lung cancer activity of Tcm X. In this study, we found that tetracenomycin X showed anti...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md17010063
更新日期:2019-01-18 00:00:00
abstract::The marine ecosystem has been a key resource for secondary metabolites with promising biological roles. In the current study, bioassay-guided phytochemical investigations were carried out to assess the presence of enzyme inhibitory chemical constituents from the methanolic extract of marine green alga-Codium dwarkense...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md13074344
更新日期:2015-07-14 00:00:00
abstract::Nowadays, various drugs on the market are becoming more and more resistant to numerous diseases, thus declining their efficacy for treatment purposes in human beings. Antibiotic resistance is one among the top listed threat around the world which eventually urged the discovery of new potent drugs followed by an increa...
journal_title:Marine drugs
pub_type: 杂志文章,评审
doi:10.3390/md15110354
更新日期:2017-11-10 00:00:00
abstract::In this study, we developed novel chitosan/fucoidan nanoparticles (CS/F NPs) using a simple polyelectrolyte self-assembly method and evaluated their potential to be antioxidant carriers. As the CS/F weight ratio was 5/1, the CS/F NPs were spherical and exhibited diameters of approximately 230-250 nm, as demonstrated b...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md12084379
更新日期:2014-07-31 00:00:00
abstract::Intermittent hypobaric hypoxia (IH) is linked with oxidative stress, impairing cardiac function. However, early IH also activate cardio-protective mechanisms. Omega 3 fatty acids (Ω3) induce cardioprotection by reducing infarct size and reinforcing antioxidant defenses. The aim of this work was to determine the combin...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md13020838
更新日期:2015-02-04 00:00:00
abstract::Two new steroids, crellasterones A (1) and B (2), together with the previously reported compound chalinasterol (3) and several nucleosides (4-7), were isolated from the sponge Crella incrustans, collected in New Caledonia. The structures of the new compounds were established by extensive NMR and mass spectroscopic ana...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md15060177
更新日期:2017-06-15 00:00:00
abstract::Carotenoids are lipid-soluble pigments that are produced in some plants, algae, fungi, and bacterial species, which accounts for their orange and yellow hues. Carotenoids are powerful antioxidants thanks to their ability to quench singlet oxygen, to be oxidized, to be isomerized, and to scavenge free radicals, which p...
journal_title:Marine drugs
pub_type: 杂志文章,评审
doi:10.3390/md13106226
更新日期:2015-09-30 00:00:00
abstract::In order to search for sulfated polysaccharides in different invertebrate connective tissues and to examine their biological activities, we have isolated three types of polysaccharides from the body wall of the three sea cucumbers Holothuria edulis, Apostichopus japonicas and Holothuria nobilis. The physicochemical pr...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md11020399
更新日期:2013-02-05 00:00:00
abstract::Ten new triterpenoid compounds with structure diversity of the C-17 side-chain, including nine tirucallanes, named xylocarpols A⁻E (1⁻5) and agallochols A⁻D (6⁻9), and an apotirucallane, named 25-dehydroxy protoxylogranatin B (10), were isolated from the mangrove plants Xylocarpus granatum, Xylocarpus moluccensis, and...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md16120488
更新日期:2018-12-06 00:00:00
abstract::Ecklonia cava (E. cava) can alleviate vascular dysfunction in diseases associated with poor circulation. E. cava contains various polyphenols with different functions, but few studies have compared the effects of these polyphenols. Here, we comparatively investigated four major compounds present in an ethanoic extract...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md16110441
更新日期:2018-11-09 00:00:00
abstract::Four new cembrane-type diterpenoids, sarcophyolides B-E (1-4), along with 11 known analogues were isolated from the soft coral Sarcophyton elegans. The structures of new compounds 1-4 were established on the basis of spectroscopic analysis and chemical conversion. The new cembranoids sarcophyolides B (1) and lobocraso...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md11093186
更新日期:2013-08-26 00:00:00
abstract::Marine invertebrates are rich sources of bioactive compounds and their biotechnological potential attracts scientific and economic interest worldwide. Although sponges are the foremost providers of marine bioactive compounds, cnidarians are also being studied with promising results. This diverse group of marine invert...
journal_title:Marine drugs
pub_type: 杂志文章,评审
doi:10.3390/md9101860
更新日期:2011-01-01 00:00:00
abstract::Curcumin (diferuloylmethane) is a natural bioactive compound with many health-promoting benefits. However, its poor water solubility and bioavailability has limited curcumin’s biomedical application. In the present study, we encapsulated curcumin into liposomes, formed from natural sources (salmon lecithin), and...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md16070218
更新日期:2018-06-25 00:00:00
abstract::The review is devoted to the chemical diversity of steroids produced by soft corals and their determined and potential activities. There are about 200 steroids that belong to different types of steroids such as secosteroids, spirosteroids, epoxy- and peroxy-steroids, steroid glycosides, halogenated steroids, polyoxyge...
journal_title:Marine drugs
pub_type: 杂志文章,评审
doi:10.3390/md18120613
更新日期:2020-12-02 00:00:00
abstract::TNF-related apoptosis-inducing ligand (TRAIL) is a tumor-selective apoptosis inducer and has been shown to be promising for treating various types of cancers. However, the application of TRAIL is greatly impeded by the resistance of cancer cells to its action. Studies show that overexpression of some critical pro-surv...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md12095072
更新日期:2014-09-25 00:00:00
abstract::Spumigins are marine natural products derived from cyanobacteria Nodulariaspumigena, which mimics the structure of the d-Phe-Pro-Arg sequence and is crucial for binding to the active site of serine proteases thrombin and factor Xa. Biological evaluation of spumigins showed that spumigins with a (2S,4S)-4-methylproline...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md16110413
更新日期:2018-10-27 00:00:00
abstract:BACKGROUND:Parenteral nutrition is often a mandatory therapeutic strategy for cases of septicemia. Likewise, therapeutic application of anti-oxidants, anti-inflammatory therapy, and endotoxin lowering, by removal or inactivation, might be beneficial to ameliorate the systemic inflammatory response during the acute phas...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md15030074
更新日期:2017-03-15 00:00:00
abstract::Fucoidan is a polysaccharide built from L-fucose molecules. The main source of this polysaccharide is the extracellular matrix of brown seaweed (Phaeophyta), but it can be also isolated from invertebrates such as sea urchins (Echinoidea) and sea cucumbers (Holothuroidea). Interest in fucoidan is related to its broad b...
journal_title:Marine drugs
pub_type: 杂志文章,评审
doi:10.3390/md17080458
更新日期:2019-08-05 00:00:00
abstract::The cultivation of macroalgae to supply the biofuel, pharmaceutical or food industries generates a considerable amount of organic residue, which represents a potential substrate for biomethanation. Its use optimizes the total resource exploitation by the simultaneous disposal of waste biomaterials. In this study, we e...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md13095947
更新日期:2015-09-18 00:00:00
abstract::Sea anemones are a remarkable source of active principles due to a decentralized venom system. New blood vessel growth or angiogenesis is a very promising target against cancer, but the few available antiangiogenic compounds have limited efficacy. In this study, a protein fraction, purified from tentacles of Anemonia ...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md16040134
更新日期:2018-04-19 00:00:00
abstract::We performed a high-content screening (HCS) assay aiming to discover bioactive molecules with proteasome inhibitory activity. By structural elucidation, we identified six compounds purified from soft coral Clavularia flava, which potentiates proteasome inhibition. Chemical structure elucidation revealed they are dolab...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md18010039
更新日期:2020-01-03 00:00:00
abstract::During an investigation of the chemistry of the Red Sea Verongiid sponge Pseudoceratina arabica, we discovered a small molecule, pseudoceratonic acid (1), along with the new moloka'iamine derivatives, ceratinines N (2), O (3), and the previously reported compounds moloka'iamine (4), hydroxymoloka'iamine (5) and cerati...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md18110525
更新日期:2020-10-23 00:00:00
abstract::Crude extracts and column fractions from the red algae Asparagopsis taxiformis and A. armata from the Strait of Messina (Italy) were screened for the production of antimicrobial compounds. Extracts from both species revealed remarkable antiprotozoal activity against Leishmania, revealing such algae as a great source o...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md7030361
更新日期:2009-08-11 00:00:00
abstract::A novel sesquiterpenoid, rumphellaoic acid A (1), was isolated from the gorgonian coral Rumphella antipathies, and was found to possess a carbon skeleton that was obtained for the first time from a natural sources. The structure of 1 was elucidated by spectroscopic methods and this compound and was found to exert a mo...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md12125856
更新日期:2014-12-04 00:00:00