Abstract:
:Considerable work is being carried out on endometrial NK cells to determine whether they play a role in successful pregnancy outcome. In addition there is debate about whether measurements of uNK should be included in the clinical assessment for women with recurrent implantation failure or recurrent miscarriage. A hindrance to taking this forward is the fact that the density of uNK cells reported by different centres is very different. The aim of this study was to determine the reason for these differences and to develop a standardised method. Three centres participated in the study. Each centre exchanged five formalin fixed, wax embedded sections of endometrium from five women. Sections were immunostained for CD56. Images were taken of 10 random fields at ×400 magnification; total stromal and uNK cells were counted using Image J. Results were expressed as % positive uNK cells and the variation in counts obtained in each centre was compared. After initial analysis a standardised protocol was agreed and the process repeated. Significant variation was seen in the counts obtained after initial analysis (Centre A vs.B, mean difference=-0.72 P<0.001; A vs.C mean difference=-0.47 P<0.001; B vs.C, mean difference=0.25 P=0.085). Analysis suggested that differences may be due to duration of tissue fixation, the embedding and sectioning processes, selection of areas for assessment, definition of immunopositive cells and inclusion or exclusion of blood vessels. Adoption of a standardised protocol reduced the variation (Centre A vs.B mean difference=-0.105 P=0.744; A vs.C mean difference=0.219 P=0.150; B vs.C mean difference=0.32 P=0.031). Use of a standardised method is needed to establish a normal range for uNK cells and to develop a meaningful clinical test for uNK cell measurements.
journal_name
J Reprod Immunoljournal_title
Journal of reproductive immunologyauthors
Lash GE,Bulmer JN,Li TC,Innes BA,Mariee N,Patel G,Sanderson J,Quenby S,Laird SMdoi
10.1016/j.jri.2016.04.290subject
Has Abstractpub_date
2016-08-01 00:00:00pages
50-9eissn
0165-0378issn
1872-7603pii
S0165-0378(16)30021-3journal_volume
116pub_type
杂志文章,多中心研究abstract::Glycodelin is a glycoprotein with 4 known glycoforms, namely glycodelin-S, glycodelin-A, glycodelin-F and glycodelin-C. The glycoforms are present in the female reproductive tract which the spermatozoa must pass through before fertilizing the oocyte. Thus the spermatozoa interact with each of these glycoforms in succe...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
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journal_title:Journal of reproductive immunology
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abstract::Establishment of pregnancy initiates a dynamic and predictable series of changes in the uterus. In rodents, the trophectoderm of the blastocyst develops through the stage of an ectoplacental cone to become the placenta. The inner cell mass becomes the fetus and its associated extra-embryonic ectoderm and mesoderm. Mat...
journal_title:Journal of reproductive immunology
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更新日期:2002-10-01 00:00:00
abstract::Sperm antigens are being sought for the development of prototype contraceptive vaccines. For vaccine-induced antisperm antibodies to inhibit the function of viable sperm, target antigens must be expressed on the sperm surface before, during or after the acrosome reaction. The purpose of this study was to develop an ap...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(91)90019-m
更新日期:1991-05-01 00:00:00
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
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journal_title:Journal of reproductive immunology
pub_type: 临床试验,杂志文章
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更新日期:2015-06-01 00:00:00
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(86)90031-8
更新日期:1986-12-01 00:00:00
abstract::In vitro fertilization (IVF) pregnancies potentially have a higher rate of preterm delivery (PTD) than do spontaneously conceived gestations, and differences persist following adjustment for multiple gestation, maternal age, and parity. The reasons for this increased susceptibility to PTD remain incompletely elucidate...
journal_title:Journal of reproductive immunology
pub_type: 临床试验,杂志文章
doi:10.1016/j.jri.2015.05.004
更新日期:2015-11-01 00:00:00
abstract::The effects of an immunosuppressive factor (ISF) isolated from boar seminal vesicle fluid on in vitro and in vivo mouse development were investigated. It was found that the zona pellucida of porcine and mouse oocytes pre-incubated with ISF reacted in indirect immunofluorescence with antibodies against ISF. Further res...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(91)90054-t
更新日期:1991-09-01 00:00:00
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/j.jri.2011.01.001
更新日期:2011-03-01 00:00:00
abstract::The feasibility of using the rhesus monkey as a non-human primate model for testing the efficacy of a contraceptive vaccine based on FA-1 antigen was evaluated. Affinity-purified anti-FA-1 polyclonal antibodies (Fab' fragments) and anti-FA-1 monoclonal antibody were used as probes in these studies. Anti-FA-1 antibodie...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(94)90027-2
更新日期:1994-10-01 00:00:00
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2008.12.006
更新日期:2009-06-01 00:00:00
abstract::The process of embryo attachment and invasion through the endometrial epithelial cells and subsequent implantation into the decidualized endometrial stroma is the groundbreaking step for the establishment of a successful pregnancy. Necessary prerequisites are a receptive endometrium, a good-quality embryo and a well-o...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/j.jri.2015.02.002
更新日期:2015-04-01 00:00:00
abstract::Human pre-implantation stage embryos cultured in vitro spontaneously secreted a factor capable of inducing histamine-release from human blood basophils. The embryo-derived histamine-releasing factor (EHRF) has been isolated from the culture medium by means of heparin-Sepharose affinity chromatography. The factor bound...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(88)90047-2
更新日期:1988-06-01 00:00:00
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(83)90020-7
更新日期:1983-01-01 00:00:00
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2007.08.003
更新日期:2008-04-01 00:00:00
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2011.05.010
更新日期:2011-09-01 00:00:00
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2019.06.004
更新日期:2019-06-01 00:00:00
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2014.04.001
更新日期:2014-12-01 00:00:00
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2008.03.005
更新日期:2008-07-01 00:00:00
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journal_title:Journal of reproductive immunology
pub_type: 历史文章,杂志文章,评审
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更新日期:2011-05-01 00:00:00
abstract::Immunoglobulin classes and subclasses of sperm immobilizing antibodies (SI-Abs) in the sera of sterile women were determined by the absorption of patients' sera with Staphylococcus aureus and immunoadsorbents bound with class or subclass-specific anti-human immunoglobulin antibodies. Among 18 patients' sera tested, 16...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(93)90077-u
更新日期:1993-08-01 00:00:00
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journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2007.03.003
更新日期:2007-08-01 00:00:00
abstract::A monoclonal anti-human sperm antibody (Mab 1A1) has been produced by fusion of myeloma cells with splenocytes from a BALB/c mouse immunized with in vitro capacitated human spermatozoa. Immunofluorescence studies with Mab 1A1 show that it recognizes an antigen(s) (Ag 1A1) which is located in the acrosome of human sper...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(91)90039-s
更新日期:1991-04-01 00:00:00
abstract::Human trophoblast membrane antigens recognised by monoclonal antibodies (H310, H315, H316 and H317) have been identified using combinations of radioimmunoprecipitation, SDS-PAGE, electroblotting, chromatographic and ELISA-type techniques. H317 is known to identify heat-stable placental-type alkaline phosphatase and ac...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/0165-0378(83)90244-9
更新日期:1983-11-01 00:00:00
abstract::Intra-testicular transplants are placed in rodents into the large lymphatic sinusoids of the interstitial tissue of the testis. These sinusoids are filled with a protein-rich extracellular fluid that supplies all the requirements of the grafts until vascularization takes place. The testicular microvascular endothelium...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/0165-0378(90)90022-x
更新日期:1990-08-01 00:00:00
abstract::We conducted a large case-control study to assess the risk of preeclampsia with elevated sTNFp55 concentrations (markers of excessive TNF-alpha release) in Peruvian women. A total of 125 women with preeclampsia and 179 normotensive women were included in a study conducted during the period, June 1997 through January 1...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/s0165-0378(99)00064-9
更新日期:2000-05-01 00:00:00
abstract::Following ejaculation, sperm functions are regulated by interactions with the environment found in the female genital tract. Here spermatozoa become 'capacitated' (i.e. they acquire the capability of completing the acrosome reaction and successfully fertilizing the egg), through a series of surface and intracellular t...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章,评审
doi:10.1016/s0165-0378(01)00089-4
更新日期:2002-01-01 00:00:00
abstract::In our earlier study, we have reported the identification and characterization of a 57-kDa sperm membrane protein from human spermatozoa. The protein was found to be localized on the acrosome in acrosome intact spermatozoa and shifted to equatorial region after acrosomal induction. Further, we demonstrated that it pla...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2005.04.004
更新日期:2005-08-01 00:00:00
abstract::Pharmacologic inhibition of indoleamine 2,3 dioxygenase (IDO) activity during murine pregnancy results in rejection of allogeneic fetuses by the maternal immune system. Here, we show that IDO expression is restricted to perinuclear regions of primary trophoblast giant cells (TGCs) of fetal origin at mid-gestation (E10...
journal_title:Journal of reproductive immunology
pub_type: 杂志文章
doi:10.1016/j.jri.2003.11.003
更新日期:2004-04-01 00:00:00