Abstract:
:The protected tripeptide tert-butyloxycarbonyl-L-leucyl-L-aminosuccinyl-L-phenylalaninamide crystallizes in the orthorhombic space group P2(1)2(1)2(1), with a = 6.214(3), b = 12.832(3), c = 33.094(4) A, Z = 4. The structure was solved by direct methods using MULTAN 80 and refined to an R value of 0.055 for 1458 reflections. The bond lengths and angles are in good agreement with the standard values. The peptide backbone adopts a type II' beta-bend conformation with a weak intramolecular hydrogen bond between the CO group of the leucyl residue and the C-terminal NH2 group. In agreement with previous studies, this structure confirms the high propensity of aminosuccinyl peptides to adopt a type II' beta-bend conformation. The role of this conformation in relation to the deamidation process in proteins is also discussed.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Capasso S,Mazzarella L,Sica F,Zagari Adoi
10.1002/bip.360280116subject
Has Abstractpub_date
1989-01-01 00:00:00pages
139-47issue
1eissn
0006-3525issn
1097-0282journal_volume
28pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::Antagonism of the calcitonin gene-related peptide (CGRP) receptor may be a useful approach for migraine treatment. Selective PEGylated peptide antagonists to the CGRP receptor are described, derived from CGRP(8-37) with polymer derivatization at an engineered lysine-25 residue. Potent PEGylated peptides with improved ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22270
更新日期:2013-07-01 00:00:00
abstract::A systematic molecular dynamics (MD) simulation has been carried out on collagen-like peptides with different combinations of interruptions in the Gly-X(AA) -Y(AA) repeats. Although experimental studies have been carried out to elucidate the structural consequences of homotrimeric collagen-like peptides, this is the f...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22085
更新日期:2012-11-01 00:00:00
abstract::A series of novel nonpeptide inhibitors of the pp60(c-Src) (Src) SH2 domain is described that exploit multifunctional group replacement of the phenylphosphate moiety of phosphotyrosine (pTyr). Relative to an x-ray structure of citrate complexed to the pTyr binding site of the Src SH2 domain, these nonpeptide ligands i...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10600
更新日期:2003-01-01 00:00:00
abstract::Although many anticancer drugs exert their biological activity by forming DNA interstrand crosslinks (ICLs), the thermodynamics of biologically relevant long crosslinked DNAs has not been intensively studied in contrast to short duplexes. Here, we carry out computer modeling of the shift of melting temperature of long...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22077
更新日期:2012-10-01 00:00:00
abstract::Seven water-insoluble (1 --> 3)-beta-D-glucan fractions TM8-1 to TM8-7 with weight-average molecular mass M(w) ranged from 2.22 to 77.4 x 10(4) obtained from the sclerotia of Pleurotus tuber-regium were carboxymethylated to produce the water-soluble fractions CTM8-1 to CTM8-7 with M(w) ranged from 3.87 to 87.8 x 10(4)...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10277
更新日期:2003-02-01 00:00:00
abstract::With the availability of accurate methods to treat the electrostatic long-range interactions, molecular dynamics simulations have resulted in refined dynamical models of the structure of the hydration shell around RNA motifs. The models reviewed here range from basic Watson-Crick to more specific noncanonical base pai...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(2000)56:4<266::AID-BIP10027>3.0.
更新日期:2000-01-01 00:00:00
abstract::Fourier transform IR (FTIR) spectroscopy has been successfully applied in recent years to examine the functional and structural properties of the membrane protein rhodopsin, a prototype G protein coupled receptor. Unlike UV-visible spectroscopy, FTIR spectroscopy is structurally sensitive. It may give us both global i...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.10407
更新日期:2003-01-01 00:00:00
abstract::Normal mode calculations have been done on a range of disulfide bridge conformations in order to determine how the SS stretch and CS stretch frequencies depend on structure. In addition to varying the C alpha C beta SS and NC alpha C beta S dihedral angles, we have varied the phi, psi of the adjoining peptide groups s...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360320813
更新日期:1992-08-01 00:00:00
abstract::Small disulfide-rich peptides are translated as larger precursors typically containing an N-terminal prepro sequence. In this study, we investigated the role of a propeptide in the oxidative folding of an extremely hydrophobic delta-conotoxin, PVIA. delta-Conotoxin PVIA (delta-PVIA) is a 29-amino acid neurotoxin stabi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20211
更新日期:2005-01-01 00:00:00
abstract::The design of biomimetic materials through molecular self-assembly is a growing area of modern nanotechnology. With problems of protein folding, self-assembly, and sequence-structure relationships as essential in nanotechnology as in biology, the effect of the nucleation of β-hairpin formation by proline on the foldin...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22622
更新日期:2015-06-01 00:00:00
abstract::The thermal conformational transitions of two sonicated samples of schizophyllan were studied in water-dimethylsulfoxide (DMSO) mixtures by high-sensitivity differential scanning calorimetry (DSC). Two transitions were observed over most of the range of solvent compositions. These were assigned to an internal change o...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360280208
更新日期:1989-02-01 00:00:00
abstract::Solid-state nmr spectroscopy provides a robust method for investigating polypeptides that have been prepared by chemical synthesis and that are immobilized by strong interactions with solid surfaces or large macroscopic complexes. Solid-state nmr spectroscopy has been widely used to investigate membrane polypeptides o...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0282(1999)51:3<174::AID-BIP2>3.
更新日期:1999-01-01 00:00:00
abstract::Electronic absorbance and fluorescence titrations are used to probe the interaction of chlorobenzylidine with DNA. The binding of chlorobenzylidine to DNA results in hypochromism, a small shift to a longer wavelength in the absorption spectra, and emission quenching in the fluorescence spectra. These spectral characte...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10001
更新日期:2001-01-01 00:00:00
abstract::The magnitude and nature of lesion-induced energetic perturbations empirically correlate with mutagenicity/cytotoxicity profiles and can be predictive of lesion outcomes during polymerase-mediated replication in vitro. In this study, we assess the sequence and counterbase-dependent energetic impact of the Thymine glyc...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22680
更新日期:2015-09-01 00:00:00
abstract::The elucidation of structure-function relationships in insoluble elastin is often approached using elastin-like polypeptides. In this manner, the characterization of the different regions in this extensive biopolymer may be facilitated in a "piece-wise" manner. Our solid-state NMR experiments indicate that (LGGVG)n ha...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10470
更新日期:2003-10-01 00:00:00
abstract::Antimicrobial peptides (AMPs) have been the focus of intense research towards the finding of a viable alternative to current small-molecule antibiotics, owing to their commonly observed and naturally occurring resistance against pathogens. However, natural peptides have many problems such as low bioavailability and hi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22885
更新日期:2016-09-01 00:00:00
abstract::The physicochemical characteristics of the green fluorescent protein (GFP), including the thermodynamic properties (entropy, enthalpy, Gibbs' free energy, heat capacity), normal mode vibrations, and atomic fluctuations, were investigated. The Gaussian 03 computational chemistry program was employed for normal mode ana...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20264
更新日期:2005-06-15 00:00:00
abstract::Cyclotides are disulfide-rich miniproteins with a circular backbone and a knotted arrangement ofdisulfide bonds. Because these plant-derived peptides are resistant to degradation and exhibit a diverse range of bioactivity they have become important agronomic and industrial objectives. They belong to a group of compoun...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21466
更新日期:2010-01-01 00:00:00
abstract::The shed epidermis (molt) of snakes comprises four distinct layers. The upper two layers, here considered as beta-layer, contain essentially beta-keratin. The following layer, known as mesos-layer, is similar to the human stratum corneum, and is formed by thin cells surrounded by intercellular lipids. The latter layer...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21184
更新日期:2009-12-01 00:00:00
abstract::Conformational analysis of the neurohypophyseal hormones oxytocin (OT) and arginine-vasopressin (AVP) has been carried out using two different computational approaches and three force fields, namely by the Electrostatically Driven Monte Carlo (EDMC) method, with the Empirical Conformational Energy Program for Peptides...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(sici)1097-0282(199602)38:2<157::aid-bip3>
更新日期:1996-02-01 00:00:00
abstract::Hybrids of the double-chain bis-cystinyl fragment 225-232/225'-232' of human immunoglobulin G1 (IgG1) with the human little-gastrin sequence 2-17 were found to induce in animals a strong antigastrin humoral immune response with antibody titers comparable to those raised with conventional gastrin/carrier-protein conjug...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360310603
更新日期:1991-05-01 00:00:00
abstract::The aminoacyl-tRNA synthetases are an ancient group of enzymes that catalyze the covalent attachment of an amino acid to its cognate transfer RNA. The question of specificity, that is, how each synthetase selects the correct individual or isoacceptor set of tRNAs for each amino acid, has been referred to as the second...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0282(1999)52:1<1::AID-BIP1>3.0.
更新日期:1999-01-01 00:00:00
abstract::Proteases play crucial roles in various biological processes, and their activities are essential for all living organisms-from viruses to humans. Since their functions are closely associated with many pathogenic mechanisms, their inhibitors or activators are important molecular targets for developing treatments for va...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.22780
更新日期:2016-11-04 00:00:00
abstract::High-level quantum-chemical and quantum-dynamics calculations are reported on the tautomerization equilibrium and rate constants of guanine and its complexes with one and two water molecules. The results are used to estimate the fraction of guanine present in the cell during DNA synthesis as the unwanted tautomer that...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(2001)61:1<77::AID-BIP10062>3.0.C
更新日期:2001-01-01 00:00:00
abstract::The open reading frame 4 (ORF 4) gene product of barley yellow dwarf virus (BYDV) may act as a movement protein (MP) by assisting the transport of viral genomic RNA across the nuclear envelope (NE) of host plant cells. To investigate interactions between BYDV MP and the NE, wild-type and mutant open reading frame (ORF...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20334
更新日期:2005-10-05 00:00:00
abstract::RADA-16-I is a self-assembling peptide which forms biocompatible fibrils and hydrogels. We used molecular dynamics simulations, atomic-force microscopy, NMR spectroscopy, and thioflavin T binding assay to examine size, structure, and morphology of RADA-16-I aggregates. We used the native form of RADA-16-I (H-(ArgAlaAs...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22755
更新日期:2016-01-01 00:00:00
abstract::The beta-subunit of the human chorionic gonadotropin (hCG) hormone, which is believed to be related to certain types of cancer, contains three hairpin-like fragments. To investigate the role of beta-hairpin formation in the early stages of the hCGbeta folding, a 28-residue peptide with the sequence RDVRFESIRLPGSPRGVNP...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(19991005)50:4<413::AID-BIP
更新日期:1999-10-05 00:00:00
abstract::The secondary structure of a 38 kDa core protein from pig skin proteodermatan sulfate (PDS), was investigated in solution using CD and Fourier transform (FT) ir spectroscopy. Both techniques generally have provided complementary data on the secondary structures of proteins. CD spectral analysis has shown that the core...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360281109
更新日期:1989-11-01 00:00:00
abstract::To optimize antigen-antibody reactions, we have synthesized chemically well-defined hydrophilic microspheres. Proteins or haptens were covalently linked to these carriers. When the microsphere conjugates were agglutinated by the corresponding antiserum, the size of the complex artificially increased during the immunol...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360320808
更新日期:1992-08-01 00:00:00
abstract::Per-O-methylated β-cyclodextrin (CD) bearing an iodoacetamide group at the 6-position was synthesized to functionalize protein surfaces. Bovine serum albumin (BSA) was quantitatively modified with the CD derivative by the S(N) 2 reaction of iodoacetamide with a cysteine residue (Cys34) on the BSA surface. The resultan...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21695
更新日期:2012-01-01 00:00:00