Abstract:
:Axial micromotion of bone fragments promotes callus formation and bone healing during the process of distraction osteogenesis (DO). This study investigated the effects of the combined axial compression and distraction (accordion) technique on bone regeneration in rat DO model. Male Sprague-Dawley rats (n = 62) underwent right tibial transverse osteotomy and were randomly divided into four groups after lengthening: control (no manipulation) and three experimental groups assigned on the basis of the period of accordion manoeuvres in the consolidation phase (Groups 1, 2, and 3 with accordion technique applied at Weeks 1, 3, and 5, respectively). Animals were terminated at 1 week after each accordion phase (i.e., Weeks 2, 4, and 6). Callus formation was monitored by X-ray radiography; new bone quality was evaluated by microcomputed tomography, histological analysis, and mechanical testing. Serum levels of hypoxia-inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) were measured. Callus formation after accordion manoeuvre at Week 3 (Group 2) increased significantly over time of consolidation. The microcomputed tomography and mechanical analysis revealed Group 2 had more newly formed bone and superior mechanical properties in contrast to the other groups at termination. Histomorphological and immunohistochemical analyses confirmed a greater degree of osteogenesis and angiogenesis corresponding to increased serum levels of HIF-1α and VEGF in Group 2. The accordion technique was effective in promoting bone consolidation via activation of HIF-1α/VEGF during DO. The accordion technique may be used in the middle phase of bone consolidation to promote bone formation in patients undergoing DO treatment.
journal_name
J Tissue Eng Regen Medjournal_title
Journal of tissue engineering and regenerative medicineauthors
Xu J,Sun Y,Wu T,Liu Y,Shi L,Zhang J,Kang Q,Chai Y,Li Gdoi
10.1002/term.2534subject
Has Abstractpub_date
2018-02-01 00:00:00pages
e1268-e1276issue
2eissn
1932-6254issn
1932-7005journal_volume
12pub_type
杂志文章abstract::Tissue engineered tracheae have been successfully implanted to treat a small number of patients on compassionate grounds. The treatment has not become mainstream due to the time taken to produce the scaffold and the resultant financial costs. We have developed a method for decellularization (DC) based on vacuum techno...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1979
更新日期:2017-03-01 00:00:00
abstract::Current cardiac cell therapies cannot effectively target and retain cells in a specific area of the heart. Cell-seeded biological sutures were previously developed to overcome this limitation, demonstrating targeted delivery with > 60% cell retention. In this study, both cell-seeded and non-seeded fibrin-based biologi...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1904
更新日期:2017-01-01 00:00:00
abstract::Macroporous calcium phosphate cement (CPC) with stem cell seeding is promising for bone regeneration. The objective of this study was to investigate the effects of co-delivering autologous bone marrow mesenchymal stem cells (BMSCs) and autologous platelet-rich plasma (PRP) in CPC scaffold for bone regeneration in mini...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2416
更新日期:2018-02-01 00:00:00
abstract::Poor angiogenesis within tissue-engineered grafts has been identified as a main challenge limiting the clinical introduction of bone tissue-engineering (BTE) approaches for the repair of large bone defects. Thick BTE grafts often exhibit poor cellular viability particularly at the core, leading to graft failure and la...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.1617
更新日期:2015-02-01 00:00:00
abstract::Microstructure in terms of hierarchical assembly exists widely in mineralized biomaterials, fulfilling an important role in setting up their outstanding properties. The purpose of this study was to investigate the hierarchical assembly of enamel structure and functions, which are related to the unique characteristics ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.21
更新日期:2007-05-01 00:00:00
abstract::Recellularization of ex vivo-derived scaffolds remains a significant hurdle primarily due to the scaffolds subcellular pore size that restricts initial cell seeding to the scaffolds periphery and inhibits migration over time. With the aim to improve cell migration, repopulation, and graft mechanics, the effects of a f...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.3015
更新日期:2020-03-01 00:00:00
abstract::The full-thickness skin wound is a common skin complication affecting millions of people worldwide. Delayed treatment of this condition causes the loss of skin function and integrity that could lead to the development of chronic wounds or even death. This study was aimed to develop a rapid wound treatment modality usi...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2842
更新日期:2019-05-01 00:00:00
abstract::In this study, clinoptilolite (CLN) was employed as a reinforcement in a polymer-based composite scaffold in bone tissue engineering and evaluated in vivo for the first time. Highly porous, mechanically stable, and osteogenic CLN/PCL-PEG-PCL (CLN/PCEC) scaffolds were fabricated with modified particulate leaching/compr...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2938
更新日期:2020-01-01 00:00:00
abstract::'Off-the-shelf' tissue-engineered skin alternatives for epidermal and dermal skin layers are available; however, no such alternative for the subdermal fat layer exists. Without this well-vascularized layer, skin graft take is variable and grafts may have reduced mobility, contracture and contour defects. In this study...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2025
更新日期:2017-04-01 00:00:00
abstract::In recent years, transforming growth factor-β3 (TGFβ3) has interested more and more researchers with its competence in engineered histogenesis. In the present study we employed recombinant adenoviral vectors to deliver the constitutively active TGFβ3 gene to human dermal fibroblasts, which could maintain the continuou...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.263
更新日期:2010-10-01 00:00:00
abstract::The aim of this study was to establish the potential of human periosteum-derived cells from elderly patients as a cell source for cartilage tissue engineering by optimizing culture conditions for both proliferation and differentiation. Periosteum was obtained from the tibiae of nine patients. Biopsies were prepared fo...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.100
更新日期:2008-08-01 00:00:00
abstract::Heart valve replacement is often the only solution for patients suffering from valvular heart disease. However, currently available valve replacements require either life-long anticoagulation or are associated with valve degeneration and calcification. Moreover, they are suboptimal for young patients, because they do ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.2326
更新日期:2018-01-01 00:00:00
abstract::Several techniques have been introduced to improve the pulp revascularization outcomes. The use of the tissue graft can create more practical tissue regeneration, provide vascular supply, and enhance tissue healing. The aim of the present study was to investigate the histologic and molecular outcomes of pulp revascula...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.3094
更新日期:2020-09-01 00:00:00
abstract::The idea of rescuing the body self-repair capability lost during evolution is progressively gaining ground in regenerative medicine. In particular, growth factors and bioactive molecules derived from activated platelets emerged as promising therapeutic agents acting as trigger for repair of tissue lesions and restorat...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2595
更新日期:2018-03-01 00:00:00
abstract::This study aims to investigate the superiorities of fast degrading elastomeric poly(glycerol sebacate) (PGS)/polycaprolactone (PCL) grafts over autologous vein grafts in the reconstruction of carotid artery, thus providing more suitable vascular grafts for carotid artery replacement. We fabricated small arterial graft...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2856
更新日期:2019-07-01 00:00:00
abstract::Keratin is an important protein used for wound healing and tissue recovery. In this study, keratin was first extracted from raw materials and chemically modified to obtain stable keratin (m-keratin). The raw and m-keratin were examined by Raman spectroscopy. The molecular weight of the m-keratin was analysed by SDS-PA...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1653
更新日期:2015-09-01 00:00:00
abstract::Bone marrow mesenchymal stem cells (MSCs) are promising candidates for cell therapy and tissue engineering. We used mesenchymal stem cells from human bone marrow (hMSCs) as the seeding cells to investigate the potential of hepatocytic differentiation of hMSCs in porous polylactic glycolic acid (PLGA) scaffolds under p...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.393
更新日期:2012-01-01 00:00:00
abstract::New injectable acrylic formulations have been prepared to be applied in restoration processes for intervertebral disks (IVDs). The solid phase of the formulations is composed of poly(methyl methacrylate) (PMMA), incorporating in some cases chondroitin sulphate (CS) as a regenerative bioactive molecule, whereas the liq...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.10
更新日期:2007-03-01 00:00:00
abstract::Autologous chondrocyte implantation (ACI) is used in 34-60% for osteoarthritic (OA) cartilage defects, although ACI is neither recommended nor designed for OA. Envisioning a hydrogel-based ACI for OA that uses chondrons instead of classically used chondrocytes, we hypothesized that human OA chondrons may outperform OA...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2516
更新日期:2018-02-01 00:00:00
abstract::The osteo-regenerative properties of allograft have recently been enhanced by addition of autogenous human bone marrow stromal cells (HBMSCs). Limitations in the use of allograft have prompted the investigation of tantalum trabecular metal (TTM) as a potential alternative. TTM is already in widespread orthopaedic use,...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1525
更新日期:2014-04-01 00:00:00
abstract::Cleft palate is one of the most common craniofacial defects in newborn babies. The characteristics of this genetic disease produce soft and hard tissue defects on the lip and maxilla, which cause not only aesthetic but also functional problems with speech, eating, and breathing. Bone grafts using autologous cancellous...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2811
更新日期:2019-02-01 00:00:00
abstract::Tissue-engineering strategies may be employed in the development of in vitro breast tissue models for use in testing regimens of drug therapies and vaccines. The physical and chemical interactions that occur among cells and extracellular matrix components can also be elucidated with these models to gain an understandi...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.162
更新日期:2009-06-01 00:00:00
abstract::Many types of cells respond to applied direct current electric fields (dcEFs) by directional cell migration, a phenomenon called galvanotaxis or electrotaxis. In this study, electrotaxis was used to control cell migration. We designed a new electrotaxis incubator and chamber system to facilitate long-term (> 12 h) obs...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1986
更新日期:2017-03-01 00:00:00
abstract::Nonvascularized fat grafting is a valuable technique for soft tissue reconstruction but poor survival of fat in the host environment remains a problem. A process known as cell-assisted transfer is used to enhance fat graft retention by adding stromal vascular fraction, an adipose-derived stem cell (ASC) rich content t...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2463
更新日期:2018-02-01 00:00:00
abstract::In clinical cases, many orthopaedists have been troubled with bone fragility, such as fractures after devitalization therapy for bone tumour, pathological fractures and metastatic tumours. The aim of this study was to determine whether loss of osteogenic capacity of devitalized autologous bones can be rescued using cu...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.67
更新日期:2008-01-01 00:00:00
abstract::Treatment of tendon-bone junction injuries is a challenge because tendon-bone interface often heals poorly and the fibrocartilage zone, which reduces stress concentration, at the interface is not formed. In this study, we used a compound called kartogenin (KGN) with platelet-rich plasma (PRP) to induce the formation o...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2258
更新日期:2017-12-01 00:00:00
abstract::Electrospinning has recently gained widespread attention as a process capable of producing nanoscale fibres that mimic native extracellular matrix. In this study, we compared the osteogenic differentiation behaviour of human adipose stem cells (ASCs) on a 3D nanofibre matrix of type I rat tail collagen (RTC) and a 2D ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.85
更新日期:2008-06-01 00:00:00
abstract::Anti-inflammatory gene therapy can inhibit inflammation driven by TNFalpha in experimental models of rheumatoid arthritis. However, assessment of the therapeutic effect on cartilage and bone quality is either missing or unsatisfactory. A multimodal imaging approach, using confocal laser scanning microscopy (CLSM) and ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.251
更新日期:2010-07-01 00:00:00
abstract::Low immunogenicity and high repopulation capacity are crucial determinants for the functional and structural performance of acellular cardiovascular implants. The present study evaluates a detergent-free, non-proteolytic, actin-disassembling regimen (BIO) for decellularization of heart valve and vessel grafts, particu...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2271
更新日期:2017-12-01 00:00:00
abstract::Despite the spontaneous regenerative capacity of the peripheral nervous system, large gap peripheral nerve injuries (PNIs) require bridging strategies. The limitations and suboptimal results obtained with autografts or hollow nerve conduits in the clinic urge the need for alternative treatments. Recently, we have desc...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2249
更新日期:2017-12-01 00:00:00