Abstract:
:Gene therapy with adenoviral early region gene (E1A) may enhance the susceptibility of neoplastic cells to chemotherapy-induced cell death. Our previous study developed a urothelium-specific oncolytic serotype 5 adenovirus (Ad5) with the uroplakin II (UPII) promoter controlling E1A expression. The present study investigated whether this urothelium-specific recombinant adenovirus (Ad5-UPII-E1A) enhanced mitomycin (MMC) and hydroxycamptothecin (HCPT) sensitization and drug-induced apoptosis in bladder cancer cells. The results of the MTT assay revealed that combination therapy, using Ad5-UPII-E1A and MMC or HCPT, synergistically inhibited the viability of bladder cancer cells in a dose- and time-dependent manner when compared with either agent alone. When cells were treated with Ad5-UPII-E1A alone they arrested in the G1 phase, but cell cycle analysis by flow cytometry revealed S phase arrest when treated with combined therapy. Treatment with MMC or HCPT enhanced Ad5-UPII-E1A-induced apoptosis in 5,637 cells, observed by transmission electron microscopy. Western blot analysis revealed that MMC and HCPT enhanced the E1A expression of the Ad5-UPII-E1A vectorin a dose-dependent manner. The present study demonstrated that Ad5-UPII-E1A combined with MMC or HCPT resulted in synergistic cytotoxicity in a process which involved the promotion of apoptosis in bladder cancer cell lines. MMC and HCPT also promoted the oncolytic effect of Ad5-UPII-E1A. Thus, treatment using Ad5-UPII-E1A combined with MMC or HCPT may be an attractive strategy for the sensitization of bladder cancer to chemotherapy.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Li S,Wang F,Zhai Z,Fu S,Lu J,Zhang H,Guo H,Hu X,Li R,Wang Z,Rodriguez Rdoi
10.3892/ol.2017.6416subject
Has Abstractpub_date
2017-08-01 00:00:00pages
2081-2088issue
2eissn
1792-1074issn
1792-1082pii
OL-0-0-6416journal_volume
14pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::Studies conducted in our lab have indicated that thalidomide cytotoxicity in the KG-1a human acute myelogenous leukemia (AML) cell line was enhanced by combining it with arsenic trioxide. The current investigation was conducted in order to evaluate the effect of thalidomide either alone or in combination with arsenic ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol_00000116
更新日期:2010-07-01 00:00:00
abstract::Chronic myeloid leukemia (CML) is a clonal hematopoietic stem cell disorder. It is characterized by the presence of the Philadelphia (Ph) chromosome, t(9;22)(q34.1;q11.2), which carries the BCR-ABL1 fusion gene. Tyrosine kinase inhibitors (TKIs) have markedly changed the treatment approach of CML and have become the f...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9497
更新日期:2018-11-01 00:00:00
abstract::Patients with the luminal B subtype of breast cancer exhibit a poor prognosis, high metastatic risk and high incidence of chemotherapy resistance. Luminal B breast cancer is sub-classified into B1 and B2. The pathophysiological mechanism of luminal B2 breast cancer (LB2BC) progression has yet to be characterized. Ther...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7256
更新日期:2017-12-01 00:00:00
abstract::The primary factor associated with poor survival rate in patients with colorectal carcinoma (CRC) is the presence of metastasis. The underlying molecular mechanisms of CRC metastasis are yet to be fully elucidated. The present study investigated the function of heat shock protein 27 (Hsp27) on the invasion and prolife...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9286
更新日期:2018-10-01 00:00:00
abstract::The aim of the present study was to investigate the optimal strategy and dosimetric measurement of thoracic radiotherapy based on three-dimensional (3D) modeling of mediastinal lymph nodes (MLNs). A 3D model of MLNs was constructed from a Chinese Visible Human female dataset. Image registration and fusion between reco...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8084
更新日期:2018-04-01 00:00:00
abstract::The soluble urokinase-type plasminogen activator receptor (suPAR) and the urokinase-type plasminogen activator receptor (uPAR) have been proposed as useful biomarkers of tumor progression. Recently, suPAR was associated with chemoresistance in lung cancer. However, its clinical significance in leukemia has not previou...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6150
更新日期:2017-07-01 00:00:00
abstract::Cervical carcinoma expressed PCNA regulatory long non-coding (lnc)RNA (CCEPR) has recently been reported to play oncogenic roles in some common types of human cancer. However, the clinical significance of CCEPR mRNA expression levels in esophageal squamous cell carcinoma (ESCC) and the exact function of CCEPR in regul...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.12006
更新日期:2020-11-01 00:00:00
abstract::Chemotherapy is widely administered to patients with advanced lung cancer; however, data regarding chemotherapeutic sensitivity are limited. The present study aimed to investigate the predictive value of inflammatory indexes for chemotherapeutic efficacy in advanced lung cancer. Patients with stage III and IV unresect...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.7781
更新日期:2018-03-01 00:00:00
abstract::Breast tissue is very sensitive to ionizing radiation due to the presence of reproductive hormones, including estrogen. In the present pilot study, the efficiency of mammography X-rays to induce DNA double strand breaks (DSB) in mammary epithelial cells was investigated. For this, freshly resected healthy breast tissu...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9024
更新日期:2018-09-01 00:00:00
abstract::Transcription factors encoded by HOX genes are vital in the determination of cell fate and identity during embryonic development. In certain malignancies, HOX genes also behave as oncogenes. The present study demonstrated suppression of the invasive tendency of glioblastoma multiforme U-118 and U-138 cells by the intr...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3893
更新日期:2016-01-01 00:00:00
abstract::The etiology of laryngeal squamous cell carcinoma (LSCC) has not yet been adequately examined. Therefore, the present study aimed to investigate the association between serum folate deficiency and abnormal expression of the cell adhesion molecule 1 (CADM1) protein in the progression of LSCC. Samples were collected fro...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.5264
更新日期:2016-12-01 00:00:00
abstract::Partial hydatidiform mole and coexisting fetus (PHMCF) is a rare condition that presents a dilemma for physicians and the parents of the fetus, particularly when PHMCF is detected during the second trimester of pregnancy. The present study reports a case of PHMCF terminated by induction of labor via administration of ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3743
更新日期:2015-12-01 00:00:00
abstract::Septin 2 (SEPT2) is a tumor-related gene belonging to the SEPT family that affects the cellular processes of hepatoma carcinoma cells, glioblastoma cells and mesangial cells and is highly expressed in breast cancer, biliary tract cancer and acute myeloid leukemia. Colorectal cancer (CRC) is the third most common type ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10528
更新日期:2019-09-01 00:00:00
abstract::Fluoro-edenite is a natural mineral species initially isolated in Biancavilla, Sicily. The fibres are similar in size and morphology to certain amphibolic asbestos fibres, the inhalation of which may cause chronic inflammation and cancer. Occupational asbestos exposure is known to be associated with pleural and lung d...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.2978
更新日期:2015-05-01 00:00:00
abstract::The present study aimed to evaluate regional liver function impairment following transcatheter arterial chemoembolization (TACE), assessed by magnetic resonance imaging (MRI) enhanced by gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA). Additionally, this study evaluated the associations betwe...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2826
更新日期:2015-03-01 00:00:00
abstract::Berberine is sourced from multiple medicinal herb resources and is easy to extract. With the advantages of low price, safety and convenience, berberine may have potential for wide clinical use. The present study aimed to investigate whether berberine inhibited the viability of colon cancer and whether it regulated the...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.7997
更新日期:2018-04-01 00:00:00
abstract::Radiofrequency ablation (RFA) and cryoablation are alternative forms of therapy used widely in various pathological states, including treatment of carcinogenesis. The reason is that ablation techniques have ability of modulating the immune system. Furthermore, recent studies have applied this form of therapy on tumor ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4997
更新日期:2016-10-01 00:00:00
abstract::Selective amino acid restriction targets mitochondria to induce apoptosis of DU145 and PC3 prostate cancer cells. Biochemical assays and flow cytometry were uitilized to analyze the glucose consumption, lactate production, pyruvate dehydrogenase (PDH), nicotinamide adenine dinucleotide (NAD)/NADH and nicotinamide aden...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.237
更新日期:2011-01-18 00:00:00
abstract::[This corrects the article DOI: 10.3892/ol.2017.6254.]. ...
journal_title:Oncology letters
pub_type: 杂志文章,已发布勘误
doi:10.3892/ol.2017.6794
更新日期:2017-10-01 00:00:00
abstract::The aim of the present study was to identify and characterize side population (SP) cells in primary cervical cancer. A primary culture was successfully established, and the SP cells were isolated via fluorescence-activated cell sorting. Subsequently, in vitro analysis of clonogenic capacity by soft agar assay and in v...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6606
更新日期:2017-09-01 00:00:00
abstract::Cyclin-dependent kinase inhibitor 2A (p16INK4a) protein is a surrogate immunohistochemical marker of human papillomavirus infection in oropharynx squamous cell carcinoma (OPSCC). However, the effects of p16INK4a in non-OPSCC require additional analysis. In addition, major gaps remain in the literature, including small...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9353
更新日期:2018-11-01 00:00:00
abstract::Certain internal malignancies, including colon cancer, can develop endobronchial metastasis. The present study reports a case of colon cancer with superficial-type endobronchial metastases in a 76-year-old male. Chest computed tomography revealed small masses and infiltrates in each lung, with bilateral hilar lymph no...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2473
更新日期:2014-11-01 00:00:00
abstract::Choroidal melanoma is a rare ocular tumor. The present study reports the case of a 66-year-old male who presented with chronic headache and progressive visual loss. Physical eye examination and combined A- and B-mode ultrasonography detected choroidal melanoma. Due to tumor characteristics the eye was enucleated resto...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8661
更新日期:2018-07-01 00:00:00
abstract::The present study selected and characterized a multidrug-resistant HL-60 human acute promyelocytic leukemia cell line, HL-60/RS, by exposure to stepwise incremental doses of doxorubicin. The drug-resistant HL-60/RS cells exhibited 85.68-fold resistance to doxorubicin and were cross-resistant to other chemotherapeutics...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7353
更新日期:2018-01-01 00:00:00
abstract::Carbonyl reductase 1 (CBR1) is theorized to participate in various cellular processes, such as signal transduction, apoptosis, carcinogenesis and drug resistance, and is highly expressed in certain malignancies, including lung tumors. Several studies have provided evidence that gene polymorphisms may affect susceptibi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6926
更新日期:2017-11-01 00:00:00
abstract::Connexins (Cxs), a conserved family of trans-membrane proteins, function in the organization of cell-cell communicatin via gap junctions in multicellular organisms. However, the role of Cxs in acute myeloid leukemia (AML) is poorly understood. In this study, we investigated the relationship between cell proliferation ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.884
更新日期:2012-11-01 00:00:00
abstract::The aim of the present study was to investigate the effect of HPV16E6 gene integration on the biological behavior of Eca109 and Eca9706 cells. This was evaluated through positive liposome transfection of HPV16E6 into Eca109 cells and Eca9706 cells. The transfection efficiency was evaluated by calculating the ratio of ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7469
更新日期:2018-02-01 00:00:00
abstract::Low-grade myofibroblastic sarcoma (LGMS) is a distinct mesenchymal myofibroblastic malignancy. The tumor may occur at a variety of sites, but is particularly associated with the head and neck. Of the two maxillary sarcomas that were analyzed in the present study, one was misdiagnosed as an inflammatory myofibroblastic...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2790
更新日期:2015-02-01 00:00:00
abstract::The detection and characterisation of tumour-derived circulating epithelial tumor cells (CETCs) or circulating tumor cells (CTCs) have been a main focus of basic oncological research over previous years. Numerous studies in the past decade have shown that CTCs are a promising tool for the estimation of the risk for me...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4878
更新日期:2016-09-01 00:00:00
abstract::Human papillomavirus (HPV) is widely known as a cause of cervical intraepithelial neoplasia (CIN) and cervical cancer. The mechanisms involved have been studied by numerous studies. The integration of the virus genome into the host cells results in the abnormal regulation of cell cycle control. HPV can also induce imm...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3295
更新日期:2015-08-01 00:00:00