Abstract:
BACKGROUND:To investigate the effect of pectinase-treated Panax ginseng (GINST) in cellular and male subfertility animal models. METHODS:Hydrogen peroxide (H2O2)-induced mouse spermatocyte GC-2spd cells were used as an in vitro model. Cell viability was measured using MTT assay. For the in vivo study, GINST (200 mg/kg) mixed with a regular pellet diet was administered orally for 4 mo, and the changes in the mRNA and protein expression level of antioxidative and spermatogenic genes in young and aged control rats were compared using real-time reverse transcription polymerase chain reaction and western blotting. RESULTS:GINST treatment (50 μg/mL, 100 μg/mL, and 200 μg/mL) significantly (p < 0.05) inhibited the H2O2-induced (200 μM) cytotoxicity in GC-2spd cells. Furthermore, GINST (50 μg/mL and 100 μg/mL) significantly (p < 0.05) ameliorated the H2O2-induced decrease in the expression level of antioxidant enzymes (peroxiredoxin 3 and 4, glutathione S-transferase m5, and glutathione peroxidase 4), spermatogenesis-related protein such as inhibin-α, and specific sex hormone receptors (androgen receptor, luteinizing hormone receptor, and follicle-stimulating hormone receptor) in GC-2spd cells. Similarly, the altered expression level of the above mentioned genes and of spermatogenesis-related nectin-2 and cAMP response element-binding protein in aged rat testes was ameliorated with GINST (200 mg/kg) treatment. Taken together, GINST attenuated H2O2-induced oxidative stress in GC-2 cells and modulated the expression of antioxidant-related genes and of spermatogenic-related proteins and sex hormone receptors in aged rats. CONCLUSION:GINST may be a potential natural agent for the protection against or treatment of oxidative stress-induced male subfertility and aging-induced male subfertility.
journal_name
J Ginseng Resjournal_title
Journal of ginseng researchauthors
Kopalli SR,Cha KM,Jeong MS,Lee SH,Sung JH,Seo SK,Kim SKdoi
10.1016/j.jgr.2015.08.005subject
Has Abstractpub_date
2016-04-01 00:00:00pages
185-95issue
2eissn
1226-8453issn
2093-4947pii
S1226-8453(15)00080-9journal_volume
40pub_type
杂志文章abstract:Background:Ginseng is a commonly used herbal medicine in treating various medical conditions. Chronic gut inflammation is a recognized factor for the development of colorectal cancer (CRC). In this project, Asian ginseng berry polysaccharide preparations were used to assess their effects on CRC and related immune regul...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2018.12.010
更新日期:2020-03-01 00:00:00
abstract::Chronic liver disease, one of the most common diseases, typically arises from nonalcoholic fatty liver disease, alcoholic liver disease, chronic viral hepatitis, or hepatocellular carcinoma. Therefore, there is a pressing need for improved treatment strategies. Korean Red Ginseng has been known to have positive effect...
journal_title:Journal of ginseng research
pub_type: 杂志文章,评审
doi:10.1016/j.jgr.2016.11.004
更新日期:2017-10-01 00:00:00
abstract::Korean red ginseng (KRG) is used worldwide as a popular traditional herbal medicine. KRG has shown beneficial effects on cardiovascular diseases, such as atherosclerosis, diabetes, and hypertension. Up-regulation of a cytoprotective protein, heme oxygenase (HO)-1, is considered to augment the cellular defense against ...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.5142/jgr.2011.35.3.352
更新日期:2011-09-01 00:00:00
abstract::Ginsenoside (ginseng saponin), the principal component of ginseng, is responsible for the pharmacological and biological activities of ginseng. We isolated lactic acid bacteria from Kimchi using esculin agar, to produce β-glucosidase. We focused on the bio-transformation of ginsenoside. Phylogenetic analysis was perfo...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.5142/jgr.2012.36.3.291
更新日期:2012-07-01 00:00:00
abstract:Background:Depression is a common neuropsychiatric disease that shows astrocyte pathology. Ginsenoside Rf (G-Rf) is a saponin found in Panax ginseng which has been used to treat neuropsychiatric diseases. We aimed to investigate antidepressant properties of G-Rf when introduced into the L-alpha-aminoadipic acid (L-AAA)...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2019.08.005
更新日期:2020-07-01 00:00:00
abstract:Background:Recently, we identified a novel ginseng-derived lysophosphatidic acid receptor ligand, called gintonin. We showed that gintonin induces [Ca2+]i transient-mediated morphological changes, proliferation, and migration in cells expressing lysophosphatidic acid receptors and that oral administration of gintonin e...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2016.12.007
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Panax ginseng has been used as Korean medicine for various diseases. It has antioxidant, hypotensive, sedative, analgesic, and endocrine activities. Dammarane-type triterpenes from the plant have various beneficial effects. METHODS:A dammarane-type triterpene saponin was isolated from P. ginseng root throug...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2016.10.002
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Although the aerial parts of hydroponic Panax ginseng are reported to contain higher contents of total ginsenosides than those of roots, the isolation and identification of active metabolites from the aerial parts of hydroponic P. ginseng have not been carried out so far. METHODS:The aerial parts of hydropo...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2014.10.005
更新日期:2015-04-01 00:00:00
abstract::An light-emitting diode (LED)-based light source was used as a monochromatic light source to determine the responses of raw ginseng roots (Panax ginseng Meyer) to specific emission spectra with respect to the production of ginsenosides. The ginsenoside content in the ginseng roots changed in response to the LED light ...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.5142/jgr.2012.36.2.198
更新日期:2012-04-01 00:00:00
abstract:Background:Panax ginseng is regarded as one of the best compounds for promoting health, and it has been used traditionally as a medicinal herb. Recently, Korean Red Ginseng (RG) has been shown to protect skin from aging and wrinkling; it can also relieve atopic dermatitis and allergy symptoms. This study aimed to evalu...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2017.12.006
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Some differences have been reported in the biotransformation of ginsenosides, probably due to the types of materials used such as ginseng, enzymes, and microorganisms. Moreover, most microorganisms used for transforming ginsenosides do not meet food-grade standards. We investigated the statistical conversion...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2015.12.008
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:The current typical drying methods for red ginseng are sun drying and hot-air drying. The purpose of this study was to investigate drying characteristics of red ginseng by using far-infrared drying. METHODS:The far-infrared drying tests on red ginseng were conducted at two drying stages: (1) high temperatur...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2015.04.001
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:Ginsenoside Rb1 (G-Rb1), the major active constituent of ginseng, improves insulin sensitivity and exerts antidiabetic effects. We tested whether the insulin-sensitizing and antidiabetic effects of G-Rb1 results from a reduction in ectopic fat accumulation, mediated by inhibition of lipolysis in adipocytes. ...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2014.11.004
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:This study was carried out to investigate the effect of the steaming process on chemical constituents, free radical scavenging activity, and antiproliferative effect of Vietnamese ginseng. METHODS:Samples of powdered Vietnamese ginseng were steamed at 120°C for various times and their extracts were subjecte...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2013.11.015
更新日期:2014-04-01 00:00:00
abstract::Longevity in medicine can be defined as a long life without mental or physical deficits. This can be prevented by Alzheimer's disease (AD). Current conventional AD treatments only alleviate the symptoms without reversing AD progression. Recent studies demonstrated that Panax ginseng extract improves AD symptoms in pat...
journal_title:Journal of ginseng research
pub_type: 杂志文章,评审
doi:10.1016/j.jgr.2017.12.008
更新日期:2018-10-01 00:00:00
abstract::The ginseng plant (Panax ginseng Meyer) has a large number of active ingredients including steroidal saponins with a dammarane skeleton as well as protopanaxadiol and protopanaxatriol, commonly known as ginsenosides, which have antioxidant, anticancer, antidiabetic, anti-adipocyte, and sexual enhancing effects. Though...
journal_title:Journal of ginseng research
pub_type: 杂志文章,评审
doi:10.5142/jgr.2013.37.261
更新日期:2013-07-01 00:00:00
abstract::We previously reported that in vitro anti-platelet activity of tissue-cultured mountain ginseng (TCMG) ethanol extracts show improved efficacy when compared with commercial ginseng products such as Korean red ginseng and Panax ginseng. However, information on the anti-platelet activity of the ethyl acetate fraction fr...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.5142/jgr.2011.35.4.442
更新日期:2011-11-01 00:00:00
abstract::Ginseng is one of the most widely used herbal medicines and is reported to have a wide range of therapeutic and pharmacological applications. Ginseng may also be potentially valuable in treating cardiovascular diseases. Research concerning cardiovascular disease is focusing on purified individual ginsenoside constitue...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.5142/jgr.2012.36.1.16
更新日期:2012-01-01 00:00:00
abstract::Jilin Province is one of the principal production bases of traditional Chinese medicine (TCM) in China with its typical preponderance in TCM resources, research and development power, and industrialization capacity. The province has 2,790 species of TCM materials in total. Over 20% of the TCM materials in common use a...
journal_title:Journal of ginseng research
pub_type: 杂志文章,评审
doi:10.1016/j.jgr.2014.05.007
更新日期:2014-10-15 00:00:00
abstract::Tissue fibrosis is an eventual pathologic change of numerous chronic illnesses, which is characterized by resident fibroblasts differentiation into myofibroblasts during inflammation, coupled with excessive extracellular matrix deposition in tissues, ultimately leading to failure of normal organ function. Now, there a...
journal_title:Journal of ginseng research
pub_type: 杂志文章,评审
doi:10.1016/j.jgr.2019.12.003
更新日期:2020-05-01 00:00:00
abstract::In this study, photosynthetic parameters such as the net photosynthesis rate, stomatal conductance, intercellular CO2 concentration, and transpiration rate were examined in selected ginseng varieties and/or lines that are resistant (Yunpoong, HTIR 1, HTIR 2, and HTIR 3) and susceptible (Chunpoong) to high temperature ...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.5142/jgr.2012.36.4.461
更新日期:2012-10-01 00:00:00
abstract::Ocotillol-type saponins are one kind of tetracyclic triterpenoids, sharing a tetrahydrofuran ring. Natural ocotillol-type saponins have been discovered in Panax quinquefolius L., Panax japonicus, Hana mina, and Vietnamese ginseng. In recent years, the semisynthesis of 20(S/R)-ocotillol-type saponins has been reported....
journal_title:Journal of ginseng research
pub_type: 杂志文章,评审
doi:10.1016/j.jgr.2017.01.001
更新日期:2017-07-01 00:00:00
abstract::Ginseng (Panax ginseng Meyer) has been shown to have anti-aging effects in animal and clinical studies. However, the molecular mechanisms by which ginseng exerts these effects remain unknown. Here, the anti-aging effect of Korean red ginseng (KRG) in rat testes was examined by system biology analysis. KRG water extrac...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.5142/jgr.2011.35.3.272
更新日期:2011-09-01 00:00:00
abstract::Our results suggested that thermal stress can lead to activation of hippocampal cell damage and reduction of memory-associated molecules in HT22 cells. These findings also provide a part of molecular rationale for the role of ginsenoside Rg5 as a potent cognitive impairment preventive compound in blocking the initiati...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2017.02.002
更新日期:2018-04-01 00:00:00
abstract:Background:Ginsenosides, which are bioactive components in ginseng, can be converted to smaller compounds for improvement of their pharmacological activities. The conversion methods include heating; acid, alkali, and enzymatic treatment; and microbial conversion. The aim of this study was to determine the bioconversion...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2017.07.004
更新日期:2019-01-01 00:00:00
abstract:Background:Panax ginseng is a well-known immune modulator, and there is concern that its immune-enhancing effects may negatively affect patients with rheumatoid arthritis (RA) by worsening symptoms or increasing the risk of adverse effects from other drugs. In this randomized, crossover clinical trial, we evaluated the...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2017.01.006
更新日期:2018-04-01 00:00:00
abstract:Background:Ginsenoside Rg3, a derivative of steroidal saponins abundant in ginseng, has a range of effects on cancer cells, including anti-cell proliferation and anti-inflammation activity. Here, we investigate two long noncoding RNAs (lncRNAs), STXBP5-AS1 and RFX3-AS1, which are hypomethylated and hypermethylated in t...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2019.02.004
更新日期:2019-10-01 00:00:00
abstract:Background:Ginsenosides are known as the principal pharmacological active constituents in Panax medicinal plants such as Asian ginseng, American ginseng, and Notoginseng. Some ginsenosides, especially the 20(R) isomers, are found in trace amounts in natural sources and are difficult to chemically synthesize. The presen...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2017.09.005
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Blind and excessive application of fertilizers was found during the cultivation of Panax notoginseng in fields, as well as increase in root rot disease incidence. METHODS:Both "3414" application and orthogonal test designs were performed at Shilin county, Yunnan province, China, for NPK (nitrogen, phosphoru...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2015.04.003
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Minor saponins or human intestinal bacterial metabolites, such as ginsenosides Rg3, F2, Rh2, and compound K, are more pharmacologically active than major saponins, such as ginsenosides Rb1, Rb2, and Rc. In this work, enzymatic hydrolysis of ginsenoside Rb1 was studied using enzyme preparations from cultured ...
journal_title:Journal of ginseng research
pub_type: 杂志文章
doi:10.1016/j.jgr.2015.05.007
更新日期:2016-04-01 00:00:00