Abstract:
:Deoxynivalenol (DON), nivalenol (NIV), T-2 toxin (T2), fumonisin B1 (FB1), zearalenone (ZEA), and moniliformin (MON) mycotoxins are common food and feed contaminants produced by Fusarium spp. However, while they are usually found to co-occur in a large range of commodities, only few data are available on mycotoxin co-exposure effects and cellular response mechanisms. In this study, the individual and combined toxic effects of these fusariotoxins were evaluated on the THP-1 human immune cell line as major fusariotoxins are mostly potent immunomodulators. In particular, four relevant fusariotoxin mixtures, namely DON-MON, DON-FB1, DON-ZEA, and NIV-T2, were studied using several parameters including cell viability as well as the expression of cell surface markers and the main mitogen-activated protein kinases (MAPKs). After 48 h exposure, a reduction of cell viability in a dose-dependent manner was observed for T2, the most cytotoxic mycotoxin, followed by NIV, DON, MON, FB1, and ZEA. Regarding mycotoxin mixtures, they mainly showed antagonism on cell viability reduction. Interestingly, at concentrations inhibiting 50% of cell viability, most viable cells exhibited surface marker loss and thus became potentially non-functional. In addition, during the first 18 h of exposure, the effects of mycotoxin mixtures on early cell apoptosis and necrosis were found to be different from those induced by the toxins alone. At the molecular level, after 1 h exposure of individual and combined mycotoxins, the three main MAPK signaling pathways (p38, SAPK/JNK, and ERK1/2) were activated, highlighting a fast reaction of the exposed cells even at low cytotoxicity levels.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Smith MC,Madec S,Troadec S,Coton E,Hymery Ndoi
10.1007/s10565-017-9408-7subject
Has Abstractpub_date
2018-06-01 00:00:00pages
191-205issue
3eissn
0742-2091issn
1573-6822pii
10.1007/s10565-017-9408-7journal_volume
34pub_type
杂志文章abstract::The lethal toxin of Bacillus anthracis, which is composed of two separate proteinaceous exotoxins, namely protective antigen and lethal factor, is central to the pathogenesis of anthrax. Low levels of this toxin are known to induce release of cytokines such as tumor necrosis factor alpha (TNF-alpha). In the present st...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1007606921569
更新日期:2000-01-01 00:00:00
abstract::The histopathological response and cell culture characteristics of liver cells from the R16 (grc-) strain of rats, which carries an MHC-linked deletion, were examined one week after a single intraperitoneal injection of 200 mg/kg body weight diethylnitrosamine (DEN) and were compared with the response of liver cells f...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00249594
更新日期:1990-04-01 00:00:00
abstract::This review summarizes the strategy of cellular immortalization based on the principle of targeted oncogenesis in transgenic mice, used to establish models of transimmortalized renal proximal tubule cells, referred to as PKSV-PCT and PKSV-PR-cells, and collecting duct principal cells, referred to as mpkCCD(cl4) cells....
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1007/s10565-006-0169-y
更新日期:2007-07-01 00:00:00
abstract::Mechanisms of cell death induced by toxins probably involve one or more processes such as inhibition of protein synthesis and impairment of plasma membrane integrity leading to an increase in membrane permeability. Since one of the possible actions of mastoparan, a cationic tetradecapeptide from wasp venom, is to pert...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00135029
更新日期:1990-01-01 00:00:00
abstract::Folic acid (FA) is a vitamin essential for normal cellular functions, growth, and development. Because humans cannot synthesize this micronutrient, it must be obtained from dietary sources through intestinal absorption. The intestinal tract is a major target for oxidative stress. Our aim was to investigate the effect ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-012-9228-8
更新日期:2012-12-01 00:00:00
abstract::Aldehyde dehydrogenase was measured in primary cultures of hepatocytes obtained with a two-step collagenase perfusion either from human hepatic tissue or from livers of Fisher rats. Basal enzyme activity declines gradually as a function of time in culture, but remains at all times higher when measured with propionalde...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00122694
更新日期:1986-06-01 00:00:00
abstract::Echinoderms are valuable test species in marine ecotoxicology and offer a wide range of biological processes appropriate for this approach. Regenerating echinoderms can be regarded as amenable experimental models for testing the effects of exposure to contaminants, particularly endocrine disrupter compounds (EDCs). As...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-008-9057-y
更新日期:2008-12-01 00:00:00
abstract::The mutagenic activity of the pyrolysis products 2-amino-3-methyl-imidazo[4,5-f]-quinoline 2-amino-3,4-dimethylimidazo[4,5-f]-quinoline in Salmonella typhimurium TA98 using rat intestinal and renal subcellular fractions as activation systems was approximately 1 and 5 revertants per nmol, respectively. This was 1,000 t...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00117825
更新日期:1987-03-01 00:00:00
abstract::The purpose of the study was to determine whether along and in combination melatonin (MLT) and pentoxifylline (PTX) exerted beneficial effects on histopathological changes and changes in oxidant and antioxidant systems in liver caused by CCl4-induced liver toxicity in mice. Mice were randomly divided into six groups: ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-006-0019-y
更新日期:2006-11-01 00:00:00
abstract::One of the major objectives at the end of this century is the development of "alternative" tests for the evaluation of the pharmacological and/or toxicological activity of newly developed molecules. Contact allergy is no exception to the rule and many research programs have been started to develop in vitro techniques ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1023/a:1007502723275
更新日期:1999-02-01 00:00:00
abstract::S-(chloroethyl)-cysteine (CEC) and S-(1,2-dichlorovinyl)-cysteine (DCVC) have been proposed as intermediates in the metabolic transformation of the carcinogens 1,2-dichloroethane and 1,1,2-trichloroethylene. We have tested the ability of CEC and DCVC to induce DNA repair and genotoxic effects at the chromosomal level ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00117768
更新日期:1988-12-01 00:00:00
abstract::We investigated mitogen-activated protein kinase (MAPK) pathways as well as reactive oxygen species (ROS) in olaquindox-induced apoptosis. Exposure of HepG2 cells to olaquindox resulted in the phosphorylation of p38 MAPK and c-Jun N-terminal kinases (JNK). To confirm the role of p38 MAPK and JNK, HepG2 cells were pret...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-013-9249-y
更新日期:2013-08-01 00:00:00
abstract::Microcystin-leucine-arginine (MC-LR) was produced by toxic cyanobacteria, which has been shown to have potent hepatotoxicity. Our previous study has proved that MC-LR were able to promote intrahepatic biliary epithelial cell excessive proliferation. However, the underlying mechanism is not yet entirely clarified. Here...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-020-09575-9
更新日期:2021-01-21 00:00:00
abstract::In pharmaceutical research, in vitro toxicity tests, for assessing the potential toxicity of new chemical entities are necessary in the early stages of the developmental process, when no information is available about the metabolism or even the target organ toxicity of the compounds to be tested. In vitro specific org...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1023/a:1010958121396
更新日期:2001-01-01 00:00:00
abstract::Lung cancer is one of the most common malignancies worldwide. Actinidia chinensis Planch root extract (acRoots) was found to have the capacity of the anti-tumor, although the molecular mechanisms remain unclear. The present study aims to investigate the molecular mechanisms by which lung cancer cells sense to inhibito...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-018-9422-4
更新日期:2018-06-01 00:00:00
abstract::The effectiveness of some chelating agents to mobilize cadmium from Chinese hamster ovary cells after chronic exposure (20 hr), as well as from cytosolic metallothionein, was studied. In the first protocol, the most effective substance was 2,3-dimercaptopropanol, followed by 2,3-dimercaptopropane-1-sulfonate and 2,3-d...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00141067
更新日期:1989-01-01 00:00:00
abstract::Skin intoxication by bis(beta-chloroethyl)sulfide (BCES; sulfur mustard) induces cutaneous lesions similar to thermal burns, characterized by slowness of skin healing. We have developed an in vitro model of skin equivalent to investigate mechanisms involved in this delay. Direct intoxication of dermal equivalent produ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1007491703271
更新日期:1998-02-01 00:00:00
abstract:OBJECTIVES:LncRNAs (long noncoding RNAs) have been reported to critically regulate colorectal cancer (CRC). We prospectively investigated effects and mechanisms of lncRNA LINC00858 on regulation of CRC progression. METHODS:Expression of LINC00858 and its target were analyzed by quantitative real-time polymerase chain ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-019-09506-3
更新日期:2020-08-01 00:00:00
abstract::The risk presented by mycotoxins is a toxicological problem. As the data given by physico-chemical analysis may be difficult to translate in terms of toxicity, however, especially when considering multiple contamination, we have developed a system for toxicological analysis of mycotoxins using cell cultures of differe...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00122655
更新日期:1989-06-01 00:00:00
abstract::The mesothelial cells (MCs) play an important role in the morpho-functional alterations of the peritoneal membrane (PM) undergoing peritoneal dialysis (PD). MCs, through the epithelial-mesenchymal transition process (EMT), progressively acquire a myofibroblast-like phenotype, promoting peritoneal fibrosis (PF) and fai...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-018-9438-9
更新日期:2019-04-01 00:00:00
abstract::The effect of noncytotoxic doses of argemone oil (AO) and butter yellow (BY), the common adulterants in edible oil, on free radical generation and signaling pathway for cell proliferation in primary cells of gall bladder (GB) was undertaken. AO and BY showed no cytotoxicity at 0.1 μl/ml and 0.1 μg/ml concentration, re...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-011-9207-5
更新日期:2012-06-01 00:00:00
abstract::A tissue system in which fibroblasts and myocytes from chick embryonic hearts were separately maintained was used to study the toxicity of ethanol. To reproduce the teratogenic effects of acute, high concentrations of ethanol typical of "binge" drinking, an open tissue culture system was employed. With open cultures, ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00119291
更新日期:1992-01-01 00:00:00
abstract::Adverse effect of nanoparticles may include impairment of phagocyte function. To identify the effect of nanoparticle size on uptake, cytotoxicity, chemotaxis, cytokine secretion, phagocytosis, oxidative burst, nitric oxide production and myeloperoxidase release, leukocytes isolated from human peripheral blood, monocyt...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-013-9265-y
更新日期:2014-02-01 00:00:00
abstract::Aging, injuries, and diseases can be considered as the result of malfunctioning or damaged cells. Regenerative medicine aims to restore tissue homeostasis by repairing or replacing cells, tissues, or damaged organs, by linking and combining different disciplines including engineering, technology, biology, and medicine...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1007/s10565-017-9384-y
更新日期:2017-08-01 00:00:00
abstract::DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) including the maintenance enzyme DNMT 1 and de novo methyltransferases DNMT 3a and DNMT 3b. Elevated levels of DNMTs have been found in cancer cells and in several types of human tumors. A polymorphism found in DNMT3b has been associated with i...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-007-9035-9
更新日期:2008-06-01 00:00:00
abstract:BACKGROUND AND AIMS:Endoplasmic reticulum (ER) stress is a growing concern for drug-induced toxicity which causes several side effects. Ritonavir, a potent HIV protease inhibitor, induces both ER and mitochondrial stress; however, the missing link between ER stress and mitochondrial damage has been unknown. In the pres...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-018-09451-7
更新日期:2019-06-01 00:00:00
abstract::Bolesatine, a glycoprotein from Boletus satanas Lenz, has previously been shown to be mitogenic in rat and human lymphocytes at very low concentrations, whereas higher concentrations inhibited protein synthesis in vitro and in several in vivo systems. The low concentrations (1-10 ng/ml) of bolesatine were shown to act...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF01305906
更新日期:1995-12-01 00:00:00
abstract::Cholestatic liver injury, a group of diseases characterized with dysregulated bile acid (BA) homeostasis, was partly resulted from BA circulation disorders, which is commonly associated with the damage of hepatocyte barrier function. However, the underlying hepatocyte barrier-protective molecular mechanisms of cholest...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-020-09567-9
更新日期:2021-01-05 00:00:00
abstract::Cyclosporin A induces in vivo a severe nephrotoxicity characterized by a large decrease in renal hemodynamics. The aim of this study is to establish the ability of the known NO donor 3-morpholinosydnonimine (SIN-1) to prevent the cyclosporin A-induced contraction by using rat isolated glomeruli and cultured glomerular...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00438167
更新日期:1996-12-01 00:00:00
abstract::In a comprehensive assessment of genotoxicity, sodium fluoride was evaluated in a battery of cellular tests providing different genetic end points and biotransformation capabilities. The tests included the following: rat hepatocyte primary culture/DNA repair assay, Salmonella typhimurium histidine locus reversion assa...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00119244
更新日期:1988-06-01 00:00:00