Abstract:
:The aim of this study was to design and develop stable nanoemulsion formulations containing IFN-γ to probe their use as an immunomodulating agent. The nanoemulsions comprising distilled water, triglycerides of capric acid/caprylic, sobitan-oleate (SP), polysorbate 80 (TW) and propylene glycol (PG) were prepared through ultra-homogenization and characterized regarding droplet size, polydispersity, surface charge, preliminary and accelerated physical stability, and rheological properties. Stable nanoemulsions were selected to incorporate nano doses of IFN-γ (100 ng.mL-1). The influence of IFN-γ incorporated nanoemulsions on functional activity of mononuclear cell for Escherichia coli enteropathogenic was analyzed through superoxide release, phagocytosis, microbicidal activity and intracellular calcium release. The optimized formulation, comprising aqueous and oily phase of 10 % and 80 %, respectively, and surfactant mix ratio (SP/TW/PG) of 3.5/5.5/1.0, remained stable in extreme conditions during 90 days, displaying oil-in-water characteristics, biocompatible pH value, significant maintenance of its rheological profile, hydrodynamic radius of 205 nm, zeta potential of -40 mV and average dose of IFN-γ 91 ng.mL- The developed formulation did not alter the MN cell viability rates. Increased the superoxide release, phagocytosis index and intracellular calcium release of MN cells of human blood. Our findings indicate that the produced formulation improved the immunomodulatory activity of the IFN-γ.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Ribeiro EB,Honorio-França AC,França EL,Soler MAdoi
10.2174/0929866523666160502155249subject
Has Abstractpub_date
2016-01-01 00:00:00pages
626-38issue
7eissn
0929-8665issn
1875-5305pii
PPL-EPUB-75345journal_volume
23pub_type
杂志文章abstract::Bucain, a potent neurotoxin isolated from the venom of the Malayan krait (Bungarus candidus), induces paralysis and death. Its crystal structure has been determined at 2.10 A resolution and based on the molecular topology and hydrophobicity profile is structurally classified as a three-fingered alpha-neurotoxin posses...
journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
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abstract::The physiological stability curve--a plot of the free energy of unfolding versus temperature--is calculated for hen egg white lysozyme from a combination of extrapolated unfolding thermodynamic data from reversible conditions and isothermal titrations with guanidine hydrochloride. The shape of the curve suggests the e...
journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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abstract::Cinnamomin is a type II ribosome-inactivating protein (RIP) and its A-chain (CTA) is a RNA N-glycosidase. It is observed that modification of tyrosine residues by N-acetylimidazole (N-AI) causes almost complete loss of CTA activity. Adenine partially protects tyrosine residues from modification by N-AI. It is proposed...
journal_title:Protein and peptide letters
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abstract::Marine species are comprising about a half of the whole global biodiversity; the sea offers an enormous resource for novel bioactive compounds. Several of the marine origin species show multifunctional bioactivities and characteristics that are useful for a discovery and/or reinvention of biologically active compounds...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
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abstract::In mammals the M1 aminopeptidase family consists of nine different proteins, five of which are integral membrane proteins. The aminopeptidases are defined by two motifs in the catalytic domain; a zinc binding motif HEXXH-(X18)-E and an exopeptidase motif GXMEN. Aminopeptidases of this family are able to cleave a broad...
journal_title:Protein and peptide letters
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更新日期:2004-10-01 00:00:00
abstract::The importance of the intestinal microbiota of animals is widely acknowledged because of its vital role in the health of animals. There are complex communities of microbiota, which colonize the gastrointestinal tract. Intestinal microbiota are conductive to animal health and the development of the host immune system. ...
journal_title:Protein and peptide letters
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abstract::During spermiogenesis, the elongating rat spermatid chromatin undergoes a gradual process of condensation which is initiated in the round spermatids at "step 7" of cytodifferentation in stage VII and extending to elongated spermatids at "step 19" of cytodifferentiation in stage VIII. The mechanism of chromatin condens...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
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更新日期:2011-08-01 00:00:00
abstract::Arginine kinase is an essential enzyme which is closely related to energy metabolism in marine invertebrates. Arginine kinase provides a significant role in quick response to environmental change and stress. In this study, we simulated a tertiary structure of Sepia pharaonis arginine kinase (SPAK) based on the gene se...
journal_title:Protein and peptide letters
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abstract::Familial mediterranean fever (FMF) is a systemic disorder characterized by recurrent attacks of fever and polyserositis. In FMF, several pro-inflammatory cytokines have been found to be elevated during the attacks. In recent years, it is shown that some proteins originated from adipose tissue play important role in in...
journal_title:Protein and peptide letters
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doi:10.2174/092986610792231555
更新日期:2010-10-01 00:00:00
abstract::The P2 protein (equivalent of L7/L12 in prokaryotes), a member of the ribosomal stalk in eukaryotes, is highly conserved, particularly its C-terminal domain. In order to understand the sequence-structure-function relationships in eukaryotic C-terminal stretches, about which nothing is known at the moment, we have inve...
journal_title:Protein and peptide letters
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更新日期:2014-01-01 00:00:00
abstract::We investigated the in vitro antibiotic activity of the 19-amino acid antimicrobial peptide HP (2-20), derived from the N-terminus of Helicobacter pylori Ribosomal Protein L1 (RPL1), against antibiotic susceptible and resistant pathogens from a patient with gallstones. HP (2-20) was active against antibiotic-susceptib...
journal_title:Protein and peptide letters
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abstract::Neuronal tau, through its proline-rich domain and the microtubule binding domain, binds to RNA non-sequence-specifically via electrostatic interaction. This binding inhibits the activity of tau. Tau and RNA were also found to co-localize in SH-SY5Y cells suggesting that RNA has opportunities to interact with tau in ce...
journal_title:Protein and peptide letters
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abstract::3-Isopropylmalate dehydrogenase (IPMDH) from Mycobacterium tuberculosis (Mtb) may be a target for specific drugs against this pathogenic bacterium. We have expressed and purified Mtb IPMDH and determined its physicalchemical and enzymological properties. Size-exclusion chromatography and dynamic light scattering measu...
journal_title:Protein and peptide letters
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abstract::Human endophilin 1 (hEndo1) is a multifunctional protein that was found to bind a wide spectrum of prolinerich endocytic proteins through its Src homology 3 (SH3) domain. In order to elucidate the unknown biological functions of hEndo1, it is essential to find out the cytoplasmic components that hEndo1 recognizes and ...
journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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abstract::A thermostable uricase identified in Bacillus firmus DWD-33, which was isolated for the first time from soil, with an apparent molecular weight of 33.5 kDa was stable against oxidants and SDS. The highest expression yields were obtained in medium containing 0.8% maltose and 1.2% soybean powder as carbon and nitrogen s...
journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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abstract::The present study dealt with the isolation, identification and characterization of pigments from red snow samples of the Quito coastal front glacier (S 62º 27,217', W 059º 45,960') in Greenwich, Archipelago South Shetland, Antarctica, during summer 2013. As a strain of Shewanella was found to be the most common and ab...
journal_title:Protein and peptide letters
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