Transcriptome Analysis of Polyhydroxybutyrate Cycle Mutants Reveals Discrete Loci Connecting Nitrogen Utilization and Carbon Storage in Sinorhizobium meliloti.

abstract：:DNA methylation in prokaryotes is widespread. The most common modification of the genome is the methylation of adenine at the N-6 position. In Escherichia coli K-12 and many gammaproteobacteria, this modification is catalyzed by DNA adenine methyltransferase (Dam) at the GATC consensus sequence and is known to modulat...

journal_title：mSystems

authors： Westphal LL,Sauvey P,Champion MM,Ehrenreich IM,Finkel SE

更新日期：2016-12-13 00:00:00

abstract：:Microbial and plant specialized metabolites, also known as natural products, are key mediators of microbe-microbe and host-microbe interactions and constitute a rich resource for drug development. In the past decade, genome mining has emerged as a prominent strategy for natural product discovery. Initially, such minin...

journal_title：mSystems

authors： Medema MH

更新日期：2018-03-06 00:00:00

abstract：:Primary production by Prochlorococcus, the smallest known free-living photosynthetic organism in terms of both physical and genomic size, is thought to have a significant role in global carbon cycles. Despite its small size and low growth rate, Prochlorococcus numerically dominates the phytoplankton community in the n...

journal_title：mSystems

authors： Szul MJ,Dearth SP,Campagna SR,Zinser ER

更新日期：2019-02-26 00:00:00

abstract：:Recent studies of mammalian microbiomes have identified strong phylogenetic effects on bacterial community composition. Bats (Mammalia: Chiroptera) are among the most speciose mammals on the planet and the only mammal capable of true flight. We examined 1,236 16S rRNA amplicon libraries of the gut, oral, and skin micr...

journal_title：mSystems

authors： Lutz HL,Jackson EW,Webala PW,Babyesiza WS,Kerbis Peterhans JC,Demos TC,Patterson BD,Gilbert JA

更新日期：2019-11-12 00:00:00

abstract：:A diverse set of prophage-mediated mechanisms protecting bacterial hosts from infection has been recently uncovered within cluster N mycobacteriophages isolated on the host, Mycobacterium smegmatis mc2155. In that context, we unveil a novel defense mechanism in cluster N prophage Butters. By using bioinformatics analy...

journal_title：mSystems

authors： Mageeney CM,Mohammed HT,Dies M,Anbari S,Cudkevich N,Chen Y,Buceta J,Ware VC

更新日期：2020-10-06 00:00:00

abstract：:The reactive intermediate deaminase RidA (EC 3.5.99.10) is conserved across all domains of life and deaminates reactive enamine species. When Salmonella entericaridA mutants are grown in minimal medium, 2-aminoacrylate (2AA) accumulates, damages several pyridoxal 5'-phosphate (PLP)-dependent enzymes, and elicits an ob...

journal_title：mSystems

authors： Borchert AJ,Gouveia GJ,Edison AS,Downs DM

更新日期：2020-03-10 00:00:00

abstract：:Acinetobacter baumannii has been reported as a multidrug-resistant bacterium due to biofilms and antimicrobial resistance mechanisms. Hence, novel therapeutic strategies are necessary to overcome A. baumannii infections. This study revealed that citral at 200 μg/ml attenuated A. baumannii biofilms by up to 90% without...

journal_title：mSystems

authors： Selvaraj A,Valliammai A,Muthuramalingam P,Sethupathy S,Subramenium GA,Ramesh M,Pandian SK

更新日期：2020-11-17 00:00:00

abstract：:Microbiology, like many areas of life science research, is increasingly data-intensive. As such, bioinformatics and data science skills have become essential to leverage microbiome sequencing data for discovery. Short intensive courses have sprung up as formal computational training opportunities at individual institu...

journal_title：mSystems

authors： Taroni JN

更新日期：2019-08-20 00:00:00

abstract：:Wastewater surveillance represents a complementary approach to clinical surveillance to measure the presence and prevalence of emerging infectious diseases like the novel coronavirus SARS-CoV-2. This innovative data source can improve the precision of epidemiological modeling to understand the penetrance of SARS-CoV-2...

journal_title：mSystems

authors： Wu F,Zhang J,Xiao A,Gu X,Lee WL,Armas F,Kauffman K,Hanage W,Matus M,Ghaeli N,Endo N,Duvallet C,Poyet M,Moniz K,Washburne AD,Erickson TB,Chai PR,Thompson J,Alm EJ

更新日期：2020-07-21 00:00:00

abstract：:Marine oil spills can impact both coastal and offshore marine environments, but little information is available on how the microbial response to oil and dispersants might differ between these biomes. Here, we describe the compositional and functional response of microbial communities to different concentrations of oil...

journal_title：mSystems

authors： Doyle SM,Lin G,Morales-McDevitt M,Wade TL,Quigg A,Sylvan JB

更新日期：2020-08-25 00:00:00

abstract：:Soil metagenomics has been touted as the "grand challenge" for metagenomics, as the high microbial diversity and spatial heterogeneity of soils make them unamenable to current assembly platforms. Here, we aimed to improve soil metagenomic sequence assembly by applying the Moleculo synthetic long-read sequencing techno...

journal_title：mSystems

authors： White RA 3rd,Bottos EM,Roy Chowdhury T,Zucker JD,Brislawn CJ,Nicora CD,Fansler SJ,Glaesemann KR,Glass K,Jansson JK

更新日期：2016-06-28 00:00:00

abstract：:The mechanisms by which microbes interact in communities remain poorly understood. Here, we interrogated specific interactions between photoautotrophic and heterotrophic members of a model consortium to infer mechanisms that mediate metabolic coupling and acclimation to partnership. This binary consortium was composed...

journal_title：mSystems

authors： Bernstein HC,McClure RS,Thiel V,Sadler NC,Kim YM,Chrisler WB,Hill EA,Bryant DA,Romine MF,Jansson JK,Fredrickson JK,Beliaev AS

更新日期：2017-03-07 00:00:00

abstract：:Dietary intake is difficult to measure reliably in humans because approaches typically rely on self-reporting, which can be incomplete and biased. In field studies of animals, DNA sequencing-based approaches such as metabarcoding have been developed to characterize diets, but such approaches have not previously been w...

journal_title：mSystems

authors： Reese AT,Kartzinel TR,Petrone BL,Turnbaugh PJ,Pringle RM,David LA

更新日期：2019-10-08 00:00:00

abstract：:In bacterial cells, inhibition of ribosomes by sublethal concentrations of antibiotics leads to a decrease in the growth rate despite an increase in ribosome content. The limitation of ribosomal activity results in an increase in the level of expression from ribosomal promoters; this can deplete the pool of RNA polyme...

journal_title：mSystems

authors： Zhang Q,Brambilla E,Li R,Shi H,Cosentino Lagomarsino M,Sclavi B

更新日期：2020-09-08 00:00:00

abstract：:Antimicrobial therapies against cystic fibrosis (CF) lung infections are largely aimed at the traditional, well-studied CF pathogens such as Pseudomonas aeruginosa and Burkholderia cepacia complex, despite the fact that the CF lung harbors a complex and dynamic polymicrobial community. A clinical focus on the dominant...

journal_title：mSystems

authors： Khanolkar RA,Clark ST,Wang PW,Hwang DM,Yau YCW,Waters VJ,Guttman DS

更新日期：2020-12-01 00:00:00

abstract：:When organisms encounter an unfavorable environment, they transition to a physiologically distinct, quiescent state wherein abundant transcripts from the previous active growth state continue to persist, albeit their active transcription is downregulated. In order to generate proteins for the new quiescent physiologic...

journal_title：mSystems

authors： López García de Lomana A,Kusebauch U,Raman AV,Pan M,Turkarslan S,Lorenzetti APR,Moritz RL,Baliga NS

更新日期：2020-07-28 00:00:00

abstract：:Here, the role of the dairy-processing chain as a reservoir of antimicrobial resistance (AR) determinants and a source of novel biocontrol quorum-sensing inhibitors is assessed through a functional metagenomics approach. A metagenomic library comprising ∼22,000 recombinant clones was built from DNA isolated from raw m...

journal_title：mSystems

authors： Alexa Oniciuc EA,Walsh CJ,Coughlan LM,Awad A,Simon CA,Ruiz L,Crispie F,Cotter PD,Alvarez-Ordóñez A

更新日期：2020-02-18 00:00:00

abstract：:The gut microbiota has been implicated in immunoglobin A nephropathy (IgAN) because the intestinal immune response is assumed to be one of the disease triggers. Since the microbial composition is heritable, we hypothesize that genetic variants controlling gut microbiota composition may be associated with susceptibilit...

journal_title：mSystems

authors： He JW,Zhou XJ,Li YF,Wang YN,Liu LJ,Shi SF,Xin XH,Li RS,Falchi M,Lv JC,Zhang H

更新日期：2021-01-12 00:00:00

abstract：:Social behavior can alter the microbiome composition via transmission among social partners, but there have been few controlled experimental studies of gut microbiome transmission among social partners in primates. We collected longitudinal fecal samples from eight unrelated male-female pairs of marmoset monkeys prior...

journal_title：mSystems

authors： Zhu L,Clayton JB,Suhr Van Haute MJ,Yang Q,Hassenstab HR,Mustoe AC,Knights D,Benson AK,French JA

更新日期：2020-03-24 00:00:00

abstract：:Although a few studies have investigated the intestinal microbiota of women with polycystic ovary syndrome (PCOS), the functional and metabolic mechanisms of the microbes associated with PCOS, as well as potential microbial biomarkers, have not yet been identified. To address this gap, we designed a two-phase experime...

journal_title：mSystems

authors： Zhang J,Sun Z,Jiang S,Bai X,Ma C,Peng Q,Chen K,Chang H,Fang T,Zhang H

更新日期：2019-04-16 00:00:00

abstract：:Globally, dental caries is the most prevalent chronic oral disease and affects roughly half of all children. The aim of this report was to use metagenomic analyses to investigate the relationship between the oral microbiome and caries in preschool children. A total of 25 preschoolers, aged 3 to 5 years old with severe...

journal_title：mSystems

authors： Wang Y,Wang S,Wu C,Chen X,Duan Z,Xu Q,Jiang W,Xu L,Wang T,Su L,Wang Y,Chen Y,Zhang J,Huang Y,Tong S,Zhou C,Deng S,Qin N

更新日期：2019-11-05 00:00:00

abstract：:Dimorphic plant and human mycopathogens require a switch from the usual yeast growth to filamentous growth for host tissue penetration, and the switch is controlled by multiple signaling systems other than the central developmental pathway. Unlike these fungi, dimorphic insect mycopathogens usually grow by hyphal exte...

journal_title：mSystems

authors： Zhang AX,Mouhoumed AZ,Tong SM,Ying SH,Feng MG

更新日期：2019-07-09 00:00:00

abstract：:Methylobacter species, members of the Methylococcales, have recently emerged as some of the globally widespread, cosmopolitan species that play a key role in the environmental consumption of methane across gradients of dioxygen tensions. In this work, we approached the question of how Methylobacter copes with hypoxia,...

journal_title：mSystems

authors： Yu Z,Pesesky M,Zhang L,Huang J,Winkler M,Chistoserdova L

更新日期：2020-01-21 00:00:00

abstract：:Fecal microbiota transplantation (FMT) is a proposedly useful strategy for the treatment of gastrointestinal (GI) disorders through remediation of the patient gut microbiota. However, its therapeutic success has been variable, necessitating research to uncover mechanisms that improve patient response. Antibiotic pretr...

journal_title：mSystems

authors： Oliphant K,Cochrane K,Schroeter K,Daigneault MC,Yen S,Verdu EF,Allen-Vercoe E

更新日期：2020-01-28 00:00:00

abstract：:Biocathodes provide a stable electron source to drive reduction reactions in electrotrophic microbial electrochemical systems. Electroautotrophic biocathode communities may be more robust than monocultures in environmentally relevant settings, but some members are not easily cultivated outside the electrode environmen...

journal_title：mSystems

authors： Eddie BJ,Wang Z,Hervey WJ 4th,Leary DH,Malanoski AP,Tender LM,Lin B,Strycharz-Glaven SM

更新日期：2017-03-28 00:00:00

abstract：:Arctic regions, which are changing rapidly as they warm 2 to 3 times faster than the global average, still retain microbial habitats that serve as natural laboratories for understanding mechanisms of microbial adaptation to extreme conditions. Seawater-derived brines within both sea ice (sea-ice brine) and ancient lay...

journal_title：mSystems

authors： Zhong ZP,Rapp JZ,Wainaina JM,Solonenko NE,Maughan H,Carpenter SD,Cooper ZS,Jang HB,Bolduc B,Deming JW,Sullivan MB

更新日期：2020-06-16 00:00:00

abstract：:It is important to expedite our understanding of antibiotic resistance to address the increasing numbers of fatalities and environmental pollution due to the emergence of antibiotic resistance and multidrug-resistant strains. Here, we combined the CRISPR-enabled trackable genome engineering (CREATE) technology and tra...

journal_title：mSystems

authors： Chen C,Choudhury A,Zhang S,Garst AD,Song X,Liu X,Chen T,Gill RT,Wang Z

更新日期：2020-04-21 00:00:00

abstract：:The identification of ancestral traits is essential to understanding the evolution of any group. In the case of parasitic groups, this helps us understand the adaptation to this lifestyle and a particular host. Most diplomonads are parasites, but there are free-living members of the group nested among the host-associa...

journal_title：mSystems

authors： Jiménez-González A,Andersson JO

更新日期：2020-12-22 00:00:00

abstract：:Vibrio anguillarum is a marine bacterium that can cause vibriosis in many fish and shellfish species, leading to high mortalities and economic losses in aquaculture. Although putative virulence factors have been identified, the mechanism of pathogenesis of V. anguillarum is not fully understood. Here, we analyzed whol...

journal_title：mSystems

authors： Castillo D,Alvise PD,Xu R,Zhang F,Middelboe M,Gram L

更新日期：2017-02-28 00:00:00

abstract：:Biological sulfur cycling in polar, low-temperature ecosystems is an understudied phenomenon in part due to difficulty of access and the dynamic nature of glacial environments. One such environment where sulfur cycling is known to play an important role in microbial metabolisms is located at Borup Fiord Pass (BFP) in ...

journal_title：mSystems

authors： Trivedi CB,Stamps BW,Lau GE,Grasby SE,Templeton AS,Spear JR

更新日期：2020-08-04 00:00:00