Abstract:
:During mammalian spermiogenesis transitional proteins and protamines replace histones on the DNA as the chromatin condenses. While previous studies suggested that histone genes are inactive postmeiotically, we have shown both by steady-state RNA analysis and nuclear run-off transcription assays that histone 2b (H2b) transcription occurs in mouse round spermatids. In addition, a novel H2b cDNA clone has been isolated from an adult mouse testes cDNA library. The sequence of this cDNA clone predicts a protein that is extremely similar to other mouse H2b proteins, except at the carboxyl-terminus where the testes H2b contains an additional 12 amino acids, seven of which are hydrophobic. In contrast to the replication-dependent histone mRNAs, the 3' untranslated region of this cDNA contains the poly(A) addition sequence (AAUAAA) upstream of a poly(A) tract. Furthermore, the conserved hairpin structure immediately upstream of replication-dependent histone mRNA termini is not present. Northern blot analysis of RNA from embryonic, ovarian, spermatogenic, and a variety of somatic tissues reveals that this novel H2b transcript is spermatid specific. The H2b mRNA is in polyribosomes isolated from spermatogenic cells, strongly suggesting that it is translated during spermiogenesis.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Moss SB,Challoner PB,Groudine Mdoi
10.1016/0012-1606(89)90299-6subject
Has Abstractpub_date
1989-05-01 00:00:00pages
83-92issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(89)90299-6journal_volume
133pub_type
杂志文章abstract::The corepressor SMRT acts on a range of transcription factors, including the retinoid and thyroid hormone nuclear receptors. The carboxy-terminal region of SMRT contains CoRNR box motifs that mediate these interactions. We have shown, in Xenopus, that SMRT can exist as isoforms containing either two or three CoRNR box...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.01.007
更新日期:2006-04-15 00:00:00
abstract::The definitive endoderm forms during gastrulation and is rapidly transformed into the gut tube which is divided along the anterior-posterior axis into the foregut, midgut, and hindgut. Lineage tracing and genetic analysis have examined the origin of the definitive endoderm during gastrulation and demonstrated that the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.10.040
更新日期:2010-01-15 00:00:00
abstract::The homebox gene Hlxb9, encoding Hb9, exhibits a dual expression profile during pancreatic development. The early expression in the dorsal and ventral pancreatic epithelium is transient and spans from embryonic day (e) 8 to e9-e10, whereas the later expression is confined to differentiating beta-cells as they appear. ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0440
更新日期:2001-12-01 00:00:00
abstract::We have isolated a cDNA of the DEAD protein family from a Xenopus ovary cDNA library. Northern hybridization revealed that the mRNA corresponding to the cDNA [XVLG1 (Xenopus vasa-like gene)] was specifically expressed in adult testis and ovary. The deduced amino acid sequence of XVLG1 was relatively homologous to that...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1093
更新日期:1994-04-01 00:00:00
abstract::The vertebrate liver and heart arise from adjacent cell layers in the anterior lateral (AL) endoderm and mesoderm of late gastrula embryos, and the earliest stages of liver and heart development are interrelated through reciprocal tissue interactions. Although classical embryological studies performed several decades ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.01.019
更新日期:2004-04-15 00:00:00
abstract::The developmental events of sporulation and fruiting body formation in the prokaryote Myxococcus xanthus are preceded by a stage of massive cell death. Two phenotypically complementable strains of M. xanthus defective in developmental lysis were identified from a group of conditional sporulation mutants. Mixture of th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90133-2
更新日期:1985-11-01 00:00:00
abstract::Dictyostelium discoideum amoebas display colonial multicellularity where starving amoebas aggregate to form migrating slugs and fruiting bodies consisting of spores and three supporting cell types. To resolve the cell signalling mechanism that control sporulation, we use insertional mutagenesis of amoebas transformed ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.04.017
更新日期:2019-08-15 00:00:00
abstract::The rabbit ovum seldom becomes polyspermic despite the presence of supernumerary sperm with easy access to the ovum plasma membrane. The purpose of this study was to characterize the role of ovum investments in blocking polyspermy. Ova were inseminated with capacitated sperm in vitro and were fertilized. Early stages ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90241-7
更新日期:1987-04-01 00:00:00
abstract::The cDNA encoding the TATA binding protein was isolated from 8- to 16-cell and morula-stage embryonic libraries of two distantly related species of sea urchin, Strongylocentrotus purpuratus and Lytechinus variegatus, respectively. The two proteins are 96% identical over both the N- and C-terminal domains, suggesting a...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9052
更新日期:1998-12-01 00:00:00
abstract::A simple assay has been developed to measure cGMP-specific phosphodiesterase (cGPD) activity in crude soluble extracts of amoebae of Dictyostelium discoideum. When amoebae of different wild-type strains were starved on buffered agar, all strains exhibited an 8- to 12-fold increase in cGMP-specific hydrolyzing activity...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90026-5
更新日期:1984-05-01 00:00:00
abstract::The Egr family of zinc-finger transcription factors, consisting of Egr1, Egr2, Egr3, and Egr4, are involved in cellular growth and differentiation. Adult Egr3-deficient mice are ataxic and lack muscle spindle proprioceptors that normally develop at the sites of Ia afferent-myotube contacts during embryogenesis. To res...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0202
更新日期:2001-04-15 00:00:00
abstract::The lizards are evolutionarily the closest vertebrates to humans that demonstrate the ability to regenerate entire appendages containing cartilage, muscle, skin, and nervous tissue. We previously isolated PAX7-positive cells from muscle of the green anole lizard, Anolis carolinensis, that can differentiate into multin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.08.037
更新日期:2018-01-15 00:00:00
abstract::The fate of bacteriophage lambda-DNA was examined after injection into the fertilized eggs of Xenopus laevis. Injection of a large amount of lambda-DNA (ca. 24 ng) into a fertilized Xenopus egg induced the formation around the injected DNA of a giant nucleus-like structure which was surrounded by an apparently normal ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90155-7
更新日期:1986-08-01 00:00:00
abstract::The organizer is an essential signaling center required for axial formation during vertebrate embryonic development. In the basal chordate amphioxus, the dorsal blastopore lip of the gastrula has been proposed to be homologous to the vertebrate organizer. Lefty is one of the first genes to be expressed in the organize...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.08.006
更新日期:2019-12-01 00:00:00
abstract::Vertebrate voluntary muscles are composed of myotubes and connective tissue cells. These two cell types have different embryonic origins: myogenic cells arise from paraxial mesoderm, while in the head many of the connective tissues are formed by neural crest cells. The objective of this research was to study interacti...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90138-7
更新日期:1986-08-01 00:00:00
abstract::A new method is presented for preparation of highly purified plasma membranes from fertilized sea urchin eggs. The purified plasma membranes are in vesicle form and are highly enriched in ouabain inhibitable, Na+/K+ ATPase activity. Analysis of membrane proteins by sodium dodecyl sulfate-gel electrophoresis indicates ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90106-9
更新日期:1983-06-01 00:00:00
abstract::Pax3/7 paired homeodomain transcription factors are important markers of muscle stem cells. Pax3 is required upstream of myod for lateral dermomyotomal cells in the amniote somite to form particular muscle cells. Later Pax3/7-dependent cells generate satellite cells and most body muscle. Here we analyse early myogenes...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.10.009
更新日期:2007-02-15 00:00:00
abstract::The nucleus of eukaryotic cells is organized into functionally specialized compartments that are essential for the control of gene expression, chromosome architecture and cellular differentiation. The mouse oocyte nucleus or germinal vesicle (GV) exhibits a unique chromatin configuration that is subject to dynamic mod...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2006.01.008
更新日期:2006-04-01 00:00:00
abstract::TGF-beta superfamily members signal through a heteromeric receptor complex to regulate craniofacial development. TGF-beta type II receptor appears to bind only TGF-beta, whereas TGF-beta type I receptor (ALK5) also binds to ligands in addition to TGF-beta. Our previous work has shown that conditional inactivation of T...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.03.045
更新日期:2008-08-01 00:00:00
abstract::The finding that retinoic acid (RA) has major effects on pattern formation in developing chick limbs has led to the conclusion that RA plays a central role in the normal development of the limb. In addition, it has been demonstrated that treatment with exogenous RA leads to changes in expression of homeobox-containing...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1301
更新日期:1994-11-01 00:00:00
abstract::Mouse oocytes at metaphase I of meiotic maturation were treated with puromycin, which caused the condensed chromosomes to become decondensed to form an interphase nucleus. The chromosomes returned to a metaphase state 6.3 hr after the oocytes were transferred to puromycin-free medium [H. J. Clarke and Y. Masui (1983) ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90006-5
更新日期:1985-03-01 00:00:00
abstract::Cardiac neural crest cells undergo extensive cell rearrangements during the formation of the aorticopulmonary septum in the outflow tract. However, the morphogenetic mechanisms involved in this fundamental process remain poorly understood. To determine the function of the Ca2+-dependent cell adhesion molecule, N-cadhe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.09.003
更新日期:2006-11-15 00:00:00
abstract::Pole cell formation in embryos of the parthenogenetic midge, Smittia sp., can be delayed or inhibited by irradiation of the posterior egg pole with ultraviolet light (uv). This leaves the schedule of nuclear divisions and chromosome eliminations virtually unaffected. However, uv irradiation delays the precocious migra...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90236-1
更新日期:1983-12-01 00:00:00
abstract::Receptor protein tyrosine phosphatases (RPTPs) have been shown to play key roles in regulating axon guidance and synaptogenesis. HmLAR2, one of two closely related LAR-like RPTPs in the embryonic leech, is expressed in a few central neurons and in a unique segmentally-iterated peripheral cell, the comb cell (CC). Here...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.05.522
更新日期:2008-08-01 00:00:00
abstract::Morphogenesis of the anterior metatarsal skin (scutate scale region), from 9.5 to 12 days of development, results in the formation of orderly patterned scale ridges. It is after the initial formation of the Definitive Scale Ridge that the characteristic outer and inner epidermal surfaces differentiate. The hard, plate...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(05)80016-8
更新日期:1991-09-01 00:00:00
abstract::Melanoblasts, the precursors of the pigment-producing cells of the skin and hair, are derived from the neural crest and migrate to the skin around 12 days of gestation in the mouse. In adult mice almost all the melanoblasts are confined to the hair follicles except for the epidermal layers of nonhairy skin. The recept...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9856
更新日期:2000-09-15 00:00:00
abstract::The HNK-1 antibody recognizes a carbohydrate moiety that is shared by a family of cell adhesion molecules and is also present on the surface of migrating neural crest cells. Here, the effects of the HNK-1 antibody on neural crest cells were examined in vitro and in vivo. When the HNK-1 antibody was added to neural tub...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90390-3
更新日期:1987-10-01 00:00:00
abstract::In Xenopus laevis, the capacity to regenerate a new lens from the outer cornea gradually decreases between stages 50 and 58, is almost negligible during the metamorphic climax, and disappears after metamorphosis. The factors responsible for lens transdifferentiation of the outer cornea are produced by the neural retin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8598
更新日期:1997-07-01 00:00:00
abstract::We have tested the role of proteoglycans in the development of feather pattern by culturing 7-day-old embryonic chick skins on medium containing para-nitrophenyl-beta-D-xyloside (2 mM). Xylosides compete with core proteins of proteoglycans by acting as exogenous acceptors for the synthesis of glycosaminoglycans leadin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90199-6
更新日期:1988-05-01 00:00:00
abstract::Neural crest cells migrate extensively through a complex extracellular matrix (ECM) to sites of terminal differentiation. To determine what role the various components of the ECM may play in crest morphogenesis, quail (Coturnix coturnix japonica) neural crest cells have been cultured in three-dimensional hydrated coll...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90094-0
更新日期:1984-08-01 00:00:00