Abstract:
:1. Mouse hippocampal neurons in dissociated culture were grown at low density on previously plated hippocampal glial cell cultures and voltage clamped using the tight seal whole-cell patch-clamp technique. Flow pipes were used to rapidly exchange the extracellular solution, and to apply N-methyl-D-aspartic acid (NMDA) and some NMDA antagonists. Fluctuation analysis was used to estimate changes in the behavior of NMDA-activated ion channels during application of antagonists. In the presence of NMDA control spectra were well fit by single Lorentzian functions consistent with mean open times of 5-6 ms. 2. Two antagonists thought to act at the NMDA receptor agonist recognition site, 2-amino-5-phosphonovaleric acid (AP5) and kynurenic acid, did not produce changes in the mean open time or single channel conductance, consistent with their action as competitive antagonists. Onset of antagonism and recovery from the action of both AP5 and kynurenic acid was rapid and complete within 1 s. However, raising the extra-cellular glycine concentration, from 1 microM to 1 mM, reduced the potency of 100 microM kynurenic acid as an NMDA antagonist, suggesting that kynurenate has an additional action as a competitive antagonist at the glycine modulatory site on NMDA receptor channels. 3. In the presence of 150 microM magnesium NMDA spectra recorded at -60 mV were fit by double Lorentzian functions, consistent with single-channel events consisting of bursts of openings lasting 3.3 ms in duration, interrupted by blocking and unblocking events of average duration 0.18 ms. The onset and recovery from magnesium antagonism was rapid, and complete within 1 s, but was highly voltage dependent and at +40 mV magnesium (150 microM) failed to produce NMDA antagonism. These results are consistent with a voltage-dependent channel block of NMDA receptor channels produced by binding of magnesium to a site within the ion channel. 4. Zinc (30 microM) was a potent NMDA antagonist at both -60 and +40 mV, and at either potential appeared to reduce the mean open time of NMDA-activated ion channels from about 5 ms to approximately 3 ms. Over the frequency range measured, 1-1,000 Hz, NMDA spectra were well fit by single Lorentzians during zinc antagonism, in contrast to results obtained with magnesium. The mean single channel conductance also decreased in the presence of zinc to approximately 75% of control. Onset of antagonism and recovery from the action of zinc was rapid and complete within 1 s.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Mayer ML,Westbrook GL,Vyklický L Jrdoi
10.1152/jn.1988.60.2.645subject
Has Abstractpub_date
1988-08-01 00:00:00pages
645-63issue
2eissn
0022-3077issn
1522-1598journal_volume
60pub_type
杂志文章abstract::Many single-unit electrophysiological studies of visual cortex have investigated strong evoked responses to simple stimuli such as oriented gratings. Experiments involving other types of stimuli, such as natural scenes, higher-order features, and surface brightness, produce single-unit responses that are more difficul...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00420.2001
更新日期:2002-05-01 00:00:00
abstract::The caudal mesopallium (CM) is a cortical-level area in the songbird auditory pathway where selective, invariant responses to familiar songs emerge. To characterize the cell types that perform this computation, we made whole cell recordings from brain slices in juvenile zebra finches ( Taeniopygia guttata) of both sex...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00694.2017
更新日期:2018-03-01 00:00:00
abstract::We studied the temporal behavior and tuning properties of medial superior temporal (MST) neurons in response to constant flow-field stimulation and continuously changing flow-field stimulation (transitions), which were obtained by morphing one flow field into another. During transitions, the flow fields resembled the ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.84.2.730
更新日期:2000-08-01 00:00:00
abstract::The physiological basis of flexion spasms in individuals after spinal cord injury (SCI) may involve alterations in the properties of spinal neurons in the flexion reflex pathways. We hypothesize that these changes would be manifested as progressive increases in reflex response with repetitive stimulus application (i.e...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00979.2001
更新日期:2003-01-01 00:00:00
abstract::The purpose of these studies was to determine the role of gracile nucleus and the effects of l-arginine-derived nitric oxide (NO) synthesis in the nucleus on the cardiovascular responses to electroacupuncture (EA) stimulation of "Zusanli" (ST36). Arterial blood pressure and heart rate were monitored during EA stimulat...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00170.2003
更新日期:2003-08-01 00:00:00
abstract::The spike-triggered averaging technique (26) was used to determine whether the synaptic input from medial gastrocnemius (MG) Ia-afferent fibers to homonymous motoneurons is "topographically weighted" (22) by means of differences in projection frequency, excitatory postsynaptic potential (EPSP) amplitude, or a combinat...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1984.51.1.64
更新日期:1984-01-01 00:00:00
abstract::1. The induction of long-term weakening of synaptic transmission in rat hippocampal slices was examined in CA1 synapses during cholinergic modulation. 2. Bath application of the cholinergic agonist carbachol (50 microM) activated an oscillation of the local field potential in the theta-frequency range (5-12 Hz), terme...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.75.2.877
更新日期:1996-02-01 00:00:00
abstract::Spinal cord neuronal circuits generate motor neuron activity patterns responsible for rhythmic hindlimb behaviors such as scratching and swimming. Kinematic analyses of limb movements generated by this motor neuron output reveal important characteristics of these behaviors. Intralimb kinematics of the turtle hindlimb ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.78.3.1394
更新日期:1997-09-01 00:00:00
abstract::Our understanding of how we learn and retain motor behaviors is still limited. For instance, there is conflicting evidence as to whether the memory of a learned visuomotor perturbation consolidates; i.e., the motor memory becomes resistant to interference from learning a competing perturbation over time. Here, we soug...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00178.2016
更新日期:2017-01-01 00:00:00
abstract::Our continuous perception of the world could be the result of discrete sampling, where individual snapshots are seamlessly fused into a coherent stream. It has been argued that endogenous oscillatory brain activity could provide the functional substrate of cortical rhythmic sampling. A new study demonstrates that cort...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00810.2017
更新日期:2018-04-01 00:00:00
abstract::N-methyl-d-aspartate receptors (NMDARs) have been linked to schizophrenia because agents that bind the receptor, like ketamine and phencyclidine, are capable of inducing schizophrenia-like symptoms. Here we show that the amino acid homocysteine (HCY), which is increased in the blood of schizophrenia patients, reduces ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00809.2012
更新日期:2013-10-01 00:00:00
abstract::In reaching to grasp an object, the arm transports the hand to the intended location as the hand shapes to grasp the object. Prior studies that tracked arm endpoint and grip aperture have shown that reaching and grasping, while proceeding in parallel, are interdependent to some degree. Other studies of reaching and gr...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00686.2015
更新日期:2015-12-01 00:00:00
abstract::The role of voltage-dependent calcium channels (VDCCs) in the nicotinic acetylcholine receptor (nAChR)-mediated enhancement of spontaneous GABAergic inhibitory postsynaptic currents (IPSCs) was investigated in chick brain slices. Whole cell recordings of neurons in the lateral spiriform (SpL) and ventral lateral genic...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1999.81.2.447
更新日期:1999-02-01 00:00:00
abstract::cAMP-dependent protein kinase (PKA) and protein kinase C (PKC) are two major modulators of synaptic transmission in the CNS but little is known about how they affect synaptic transmission in the human CNS. In this study, we used forskolin, a PKA activator, and phorbol ester, a PKC activator, to examine the effects of ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01031.2002
更新日期:2003-05-01 00:00:00
abstract::A number of studies have indicated an important role for N-methyl-D-aspartate (NMDA) receptors in cell survival versus cell death decisions during neuronal development, trauma, and ischemia. Coupling of neurons by electrical synapses (gap junctions) is high or increases in neuronal networks during all three of these c...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00362.2007
更新日期:2007-11-01 00:00:00
abstract::Adaptation of movement may be driven by the difference between planned and actual motor performance, or the difference between expected and actual sensory consequences of movement. To identify how the nervous system differentially uses these signals, we asked: does motor adaptation occur when movement errors are irrel...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00273.2011
更新日期:2012-02-01 00:00:00
abstract::In acute experiments performed in decerebrated and spinalized (T13) cats, an intraspinal injection of clonidine, a noradrenergic agonist, restricted to mid-lumbar segments L3-L4, can induce hindlimb locomotion, whereas yohimbine, a noradrenergic antagonist, can block spinal locomotion, and a second spinal lesion at L4...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00909.2004
更新日期:2005-05-01 00:00:00
abstract::Upon prolonged viewing of a sinusoidal grating, the visual system is selectively desensitized to the spatial frequency of the grating, while the sensitivity to other spatial frequencies remains largely unaffected. This technique, known as pattern adaptation, has been so central to the psychophysical study of the mecha...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00216.2009
更新日期:2009-10-01 00:00:00
abstract::Autoinhibitory serotonin 1A receptors (5-HT(1A)) in dorsal raphé nucleus (DRN) have been implicated in chronic depression and in actions of selective serotonin reuptake inhibitors (SSRI). Due to experimental limitations, it was never studied at single-cell level whether changes in 5-HT(1A) receptor functionality occur...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00109.2007
更新日期:2007-07-01 00:00:00
abstract::Exercise, in the form of moderate daily treadmill training following nerve transection and repair leads to enhanced axon regeneration, but its effect on functional recovery is less well known. Female rats were exercised by walking continuously, at a slow speed (10 m/min), for 1 h/day on a level treadmill, beginning 3 ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00946.2012
更新日期:2013-06-01 00:00:00
abstract::1. Neurons (n = 142) were recorded with extracellular micropipettes in the parabrachial (PB) area, in the anesthetized rat, some of them being antidromically driven from the centralis nucleus of the amygdala (Ce). The spontaneous activity of these neurons was low, (10th percentile < median frequency < 90th percentile;...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.71.5.1646
更新日期:1994-05-01 00:00:00
abstract::Stimulus-specific adaptation is the phenomenon whereby neural response magnitude decreases with repeated stimulation. Inconsistencies between recent nonhuman animal recordings and computational modeling suggest dynamic influences on stimulus-specific adaptation. The present human electroencephalography (EEG) study inv...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00634.2014
更新日期:2015-04-01 00:00:00
abstract::Intracellular recordings were made from rhythm-generating interneurons in the Lymnaea feeding system. The feeding pattern is a three-phase rhythm of interneuronal activity (N1, N2, N3) corresponding to protraction, rasp, and swallow. We describe the firing pattern and anatomy of the premotor interneurons, each of whic...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1985.54.6.1396
更新日期:1985-12-01 00:00:00
abstract::Saccadic eye movements of different sizes and directions are represented in an orderly topographic map across the intermediate and deep layers of the superior colliculus (SC), where large saccades are encoded caudally and small saccades rostrally. Based on experiments in the cat, it has been suggested that saccades ar...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2002.87.6.2778
更新日期:2002-06-01 00:00:00
abstract::1. Opaline release in Aplysia provides a simple model system for examining the biochemical and electrophysiological mechanisms underlying glandular secretion and its modulation. The opaline gland is a large multivesicular structure, which is innervated by at least three large identified motor neurons located within th...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1982.48.6.1347
更新日期:1982-12-01 00:00:00
abstract::GSG1L is an AMPA receptor (AMPAR) auxiliary subunit that regulates AMPAR trafficking and function in hippocampal CA1 pyramidal neurons. However, its physiological roles in other types of neurons remain to be characterized. Here, we investigated the role of GSG1L in hippocampal dentate granule cells and found that GSG1...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00307.2016
更新日期:2017-01-01 00:00:00
abstract::Monitoring representative fractions of neurons from multiple brain circuits in behaving animals is necessary for understanding neuronal computation. Here, we describe a system that allows high-channel-count recordings from a small volume of neuronal tissue using a lightweight signal multiplexing headstage that permits...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00785.2013
更新日期:2014-03-01 00:00:00
abstract::Overestimation of roll tilt in hypergravity ("G-excess" illusion) has been demonstrated, but corresponding sustained hypogravic conditions are impossible to create in ground laboratories. In this article we describe the first systematic experimental evidence that in a hypogravity analog, humans underestimate roll tilt...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00140.2018
更新日期:2018-12-01 00:00:00
abstract::Many critically ill patients in intensive care units suffer from an infection-induced whole body inflammatory state known as sepsis, which causes severe weakness in patients who survive. The mechanisms by which sepsis triggers intensive care unit-acquired weakness (ICUAW) remain unclear. Currently, research into ICUAW...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00936.2012
更新日期:2013-04-01 00:00:00
abstract::Neocortical circuits exhibit a rich dynamic repertoire, and their ability to achieve entrainment (adjustment of their frequency to match the input frequency) is thought to support many cognitive functions and indicate functional flexibility. Although previous studies have explored the influence of various circuit prop...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00401.2019
更新日期:2020-03-01 00:00:00