Cyclin mRNA and protein expression in recombinant interleukin 2-stimulated cloned murine T lymphocytes.

Abstract:

:Expression of cyclin, a non-histone nuclear protein, during recombinant interleukin 2 (rIL2)-driven cell-cycle progression of cloned T lymphocytes has been assessed. We found that expression of cyclin protein, as detected by immunofluorescence, is tightly associated with proliferation, and not merely S-phase, of L2 cells stimulated with rIL2. Cyclin immunofluorescence was detected in all cell-cycle phases (G1/S/G2/M, as detected by flow cytometry) of proliferating L2 cells. Accumulation of cyclin mRNA levels was induced as early as 1 h after stimulation, was maximal at 25-49 h, and remained elevated throughout stimulation, as detected by Northern blot analysis. A cDNA-encoding murine cyclin was cloned from a cDNA library prepared from IL2-stimulated cloned T cells. The sequence of the 5' end of the murine cyclin cDNA was determined and found to be 88% and 82% similar to the sequences of cDNA clones encoding rat and human cyclin, respectively. The present studies demonstrate that cyclin protein and mRNA accumulation are highly regulated during IL2-induced proliferation of a cloned T cell. These data provide a framework for addressing the molecular mechanisms regulating cyclin gene expression during cellular proliferation.

journal_name

J Cell Biochem

authors

Shipman PM,Sabath DE,Fischer AH,Comber PG,Sullivan K,Tan EM,Prystowsky MB

doi

10.1002/jcb.240380306

subject

Has Abstract

pub_date

1988-11-01 00:00:00

pages

189-98

issue

3

eissn

0730-2312

issn

1097-4644

journal_volume

38

pub_type

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