Abstract:
:Hirschsprung's disease (HSCR) is a congenital malformation characterized by the absence of enteric ganglia in the distal intestine and gut obstruction. Some HSCR patients also have associated neurological symptoms. We studied a rat model of HSCR, also known as spotting lethal (sl/sl) rat, which carries a spontaneous deletion in the gene of endothelin receptor B (EDNRB) and a similar phenotype as humans with HSCR. We focused on the changes in cell proliferation and apoptosis in the hippocampal formation of the sl/sl rat. Proliferating cells in wildtype (+/+), heterozygous (+/sl) and homozygous (sl/sl) rats were labelled by intraperitoneal injection of 5-bromo-2'-deoxyuridine (BrdU) at postnatal day 2. The density of proliferating cells in the CA1 and CA3 regions of the hippocampus and dentate gyrus of sl/sl rats was significantly reduced compared to +/+ rats. The effect of EDNRB mutation on cell apoptosis was examined by using terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling assay. This showed that the density of apoptotic cells in the hippocampal formation, particularly in the CA1 region of sl/sl rats, was significantly increased compared to +/+ rats. The expression of brain derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) was measured with ELISA in the hippocampal formation, but no difference was revealed between genotypes. These results suggest that EDNRB mutation reduces cell proliferation and increases apoptosis in the hippocampal formation of the sl/sl rat, but does not alter the levels of BDNF and GDNF. Our findings provide an insight into the cellular changes in the brains of HSCR patients caused by EDNRB mutation.
journal_name
Brain Resjournal_title
Brain researchauthors
Xie D,Croaker GDH,Li J,Song ZMdoi
10.1016/j.brainres.2016.03.024subject
Has Abstractpub_date
2016-07-01 00:00:00pages
79-86eissn
0006-8993issn
1872-6240pii
S0006-8993(16)30151-2journal_volume
1642pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::30-Day-old male Wistar rats were tested for acquisition and retention of operant conditioned behavior after bilateral subicular lesions made either electrolytically or chemically (ibotenic acid). The acquisition of operant learning was carried out in lesioned rats by assessing the number of sessions required to learn ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(96)01135-3
更新日期:1997-01-16 00:00:00
abstract::It has been hypothesized that mitochondrial respiratory chain dysfunction leads to a pyrimidine deficiency since the pyrimidine biosynthetic enzyme dihydroorotate dehydrogenase is coupled to the electron transport chain. The uridine prodrug triacetyluridine (PN401) is neuroprotective in several models of neurodegenera...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.10.056
更新日期:2005-12-20 00:00:00
abstract::Norepinephrine (NE), acting on brain adrenoceptors, plays an important role in barrel field neuronal activity and plasticity. For this reason, the distribution of alpha 1- and alpha 2-adrenoceptors in the somatosensory cortex barrel field was studied by autoradiographic techniques in rats undergoing plastic change or ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90005-8
更新日期:1993-09-24 00:00:00
abstract::Dendritic architecture and distribution of dendritic protrusions were studied on Golgi-impregnated neurons in the medial preoptic area (MPOA) of infant rats (7 and 20 days old), of animals at puberty (34 days old) and of postpubertal rats (90 days old) using computerized image analysis. The protrusions showed a peak d...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(86)90006-4
更新日期:1986-04-01 00:00:00
abstract::Nicergoline, a drug used for the treatment of Alzheimer's disease and other types of dementia, was tested for its ability to protect neurons against beta-amyloid toxicity. Pure cultures of rat cortical neurons were challenged with a toxic fragment of beta-amyloid peptide (betaAP(25-35)) and toxicity was assessed after...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.04.004
更新日期:2005-06-14 00:00:00
abstract::Toxoplasma gondii can cause parasitic encephalitis, a life-threatening infection that predominately occurs in immunocompromised individuals. T. gondii has the ability to invade the brain, but the mechanisms by which this parasite crosses the blood-brain-barrier (BBB) remain incompletely understood. The present study r...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2020.147002
更新日期:2020-11-01 00:00:00
abstract::Opioid modulation of ingestion includes general opioid antagonism of deprivation-induced water intake and intake of sucrose and saccharin solutions. Previous studies using selective subtype antagonists indicated that opioid effects upon deprivation-induced water intake occurred through the mu2 receptor and that opioid...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91274-v
更新日期:1993-08-06 00:00:00
abstract::The current experiment aimed to explore age differences in brain activity associated with successful memory retrieval in older adults with different levels of executive functioning, at different levels of task demand. Memory performance and fMRI activity during a recognition task were compared between a young group an...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2015.10.009
更新日期:2016-01-15 00:00:00
abstract::The effect of different dose, mode and duration of estradiol administration was examined in the different brain catecholaminergic areas in ovariectomized (OVX) female rats. We determined changes in mRNA levels of tyrosine hydroxylase (TH), rate-limiting enzyme in catecholamine (CA) biosynthesis of GTP cyclohydrolase I...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.04.002
更新日期:2004-07-23 00:00:00
abstract::Bilateral microinjections of the neuroleptic, cis-flupenthixol, were made into 56 forebrain targets distributed across various dopamine (DA) terminal fields in the forebrain. Drug effects on medial forebrain bundle (MFB) stimulation-produced reward were assessed with a rate-frequency procedure implemented in a runway ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)91400-5
更新日期:1989-01-16 00:00:00
abstract::Beta-amyloid (Abeta) peptides, the major component of senile plaques in brains of patients with Alzheimer's disease (AD), were found in the low pH organelles (i.e. endosome/lysosome) of cultured neuronal cells. Since acidic pH values have been shown to promote the self-assembly of Abetas, which seems to be a prerequis...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)03322-9
更新日期:2001-03-02 00:00:00
abstract::The functional role of many central nervous structures has been inferred from the temporal relationship of a neuronal response with the different sensory and motor events in an experimental design such as when an animal performs a trained movement in response to a conditioned stimulus. However, this kind of data analy...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90279-2
更新日期:1983-11-14 00:00:00
abstract::Growth of cultured N1E-115 neuroblastoma cells in 1 microM A23187 for 2 days to elevate internal Ca reduced both membrane Na current and the transient, but not steady state, component of outward K current. Na channel mRNA abundance was reduced by an average value of 45% without effect on Kv3.1. Increases in internal C...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)01340-7
更新日期:1996-01-15 00:00:00
abstract::Since it has been proposed that oxidized protein accumulation plays a critical role in brain aging, we have investigated their contents in synaptic mitochondria from five age groups of mice. Protein carbonyl content in synaptic mitochondria showed a significant positive correlation with age (r = 0.95, P = 0.01). A lin...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(96)00708-1
更新日期:1996-08-26 00:00:00
abstract::We investigated the brain-stem pathway(s) by which electrical stimulation of the central cut end of the lingual nerve (LN) evokes parasympathetic reflex vasodilatation in the palate contralateral to the stimulated side. This occurs in artificially ventilated, cervically vagosympathectomized cats deeply anesthetized wi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.06.010
更新日期:2004-09-10 00:00:00
abstract::An extract of fetal calf striated muscle was found to support the survival, growth and differentiation of chick ciliary ganglion neurons in dissociated cell culture. The active material was precipitated in a single fraction by 35-60% ammonium sulfate. When this active fraction was passed over a concanavalin A-Sepharos...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(85)90229-9
更新日期:1985-03-01 00:00:00
abstract::An acute pain animal model for fMRI study would provide useful spatial and temporal information for studying the supraspinal nociceptive neuronal responses. The aim of the present study was to investigate whether the nociceptive responses in different brain areas can be differentiated by using functional magnetic reso...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)02094-7
更新日期:2001-04-06 00:00:00
abstract::Neurotrophins are potent regulators of the survival of different neuronal populations in the CNS. Little is known of the immunodistribution of neurotrophin-3 (NT-3) and tyrosine kinase C (TrkC) receptor in the frog visual system, which can successfully regenerate and recover vision after injury. In this study we show ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(03)02948-2
更新日期:2003-08-22 00:00:00
abstract::Chemical (C) kindling by means of repeated spaced injections into the amygdala (AM) of a subconvulsive dose of L-glutamate and L-aspartate combined in a molar ratio of 1:3. (Glu/Asp) produced progressive seizure development culminating in generalized convulsion strikingly similar to electrically kindled Stage 5 seizur...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90481-1
更新日期:1987-11-03 00:00:00
abstract::The periaqueductal gray was shown to be an important component of morphine analgesia and tolerance. Two-way analgesic cross tolerance was obtained between systemic and intracerebral morphine administrations when the intracerebral site was the periaqueductal gray. Rats were pretreated with intraperitoneal morphine and ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(76)90448-0
更新日期:1976-02-27 00:00:00
abstract::Neural stem cells can be derived from the adult/embryonic nervous system as well as from more primitive embryonic stem cells but, because of the lack of specific markers, only their differentiated progeny can be characterized. We here report the presence of several endothelial and hematopoietic receptors (at protein a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)03291-7
更新日期:2002-01-25 00:00:00
abstract::The experimental evidence linking glutamate to ischemic neuronal injury is derived from in vitro or in vivo animal stroke models. We, therefore, developed an in vitro preparation to determine whether glutamate contributes to early neuronal swelling in oxygen and glucose deprived (OGD) human neocortical slices. In orde...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)01286-9
更新日期:1998-01-26 00:00:00
abstract::Astrocytes produce and bind endothelins (ETs), suggesting that these cells have ET autoregulatory and eliminatory functions. To further investigate these functions in primary rat astrocytes, ET-1 levels in the cell culture media (RIA/HPLC) and intracellular content of ET-1 mRNA (RT PCR) were measured under basal and s...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)01368-1
更新日期:1998-03-02 00:00:00
abstract::The present study utilized the [14C]2-deoxyglucose (2-DG) cell labeling procedure to characterize a functional pathway from the prefrontal cortex (Pfc) and mediodorsal thalamic nucleus (MD) to the hypothalamus. Rats were injected with 2-DG prior to a 45 min experimental paradigm consisting of alternating 30 s on-off p...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90151-3
更新日期:1984-09-24 00:00:00
abstract::Although cocaine shares the ability of fenfluramine to inhibit the synaptic reuptake of serotonin, previous observations from our group suggest that the genomic effects of fenfluramine in the rat striatum are primarily mediated by dopaminergic rather than serotonergic mechanisms. To compare and further understand the ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91391-9
更新日期:1994-05-30 00:00:00
abstract::17β-estradiol (E2 or estrogen) is an endogenous steroid hormone that is well known to exert neuroprotection. Along these lines, one mechanism through which E2 protects the hippocampus from cerebral ischemia is by preventing the post-ischemic elevation of Dkk1, a neurodegenerative factor that serves as an antagonist of...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2012.12.015
更新日期:2013-06-13 00:00:00
abstract::Explosive running is a reliable initial component of audiogenic seizures (AS) induced by acoustic stimulation in genetically prone rodents. This profound locomotor activation is usually considered as a convulsive manifestation of AS although some studies attribute running to a panic-like response. Increase in central ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.02.017
更新日期:2007-05-07 00:00:00
abstract::In this study, we observed the chronological alterations of neurofilament 150 (NF-150) immunoreactivity in the gerbil hippocampus and dentate gyrus after 5 min transient forebrain ischemia. NF-150 immunoreactivity in the sham-operated group was mainly detected in mossy fibers and in the hilar region of the dentate gyr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.05.001
更新日期:2004-07-30 00:00:00
abstract::The right wingbuds of stage 23-25 chick embryos were amputated at the future elbow region and a segment of 2-day neural tube was implanted longitudinally into the limb stump of experimental embryos to induce limb regeneration. Control embryos had no implant in the amputated limb stump. To analyze effects of the neural...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(85)90196-8
更新日期:1985-04-01 00:00:00
abstract::This study examined whether phenidone, a dual inhibitor of cyclooxygenase (COX) and lipoxygenase (LOX), affects the clinical symptoms of experimental autoimmune encephalomyelitis (EAE) in the rat, and the expression of both COX-1/-2 and 5-LOX in EAE spinal cords. Oral phenidone (200 mg/kg) significantly suppressed the...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.12.017
更新日期:2005-02-28 00:00:00