Abstract：
:Human adenoviruses are responsible for a wide range of clinical infections and are present in aquatic environments, including river, seawater, drinking-water and sewage. Free-living amoebae (Acanthamoeba) in the same environments may internalize them and other microorganisms can act as a reservoir for the internalized viruses. In this study, we studied the interaction between Acanthamoeba polyphaga and Human Adenovirus type 5 (HAdV 5) to determine whether the amoeba played a role in protecting the internalized viruses from chemical disinfection. The efficacy of sodium hypochlorite disinfection against A. polyphaga and HAdV 5 either singly or in combination was assessed at three different concentrations. Individually, the amoeba were more resistant to chemical disinfection than HAdV 5 and remained alive after exposure to 5mg/l of sodium hypochlorite. In contrast, HAdV 5 lost infectivity following exposure to 2.5mg/l of sodium hypochlorite. When the amoeba and HAdV 5 were co-cultured, infectious virus was found in the cytoplasm of the amoeba at 5mg/l disinfectant concentration. These findings suggest that the A. polyphaga is providing protection for the HAdV 5.
journal_name
Eur J Protistoljournal_title
European journal of protistologyauthors
Verani M,Di Giuseppe G,Tammaro C,Carducci Adoi
10.1016/j.ejop.2016.02.006subject
Has Abstractpub_date
2016-06-01 00:00:00pages
11-8eissn
0932-4739issn
1618-0429pii
S0932-4739(16)30006-2journal_volume
54pub_type
杂志文章abstract：:Hyalophysa clampi Browning and Landers, 2012 was reexamined to determine all stages in the life cycle of this symbiotic ciliate. The cell feeds as a normal exuviotroph within the exoskeleton of its molted crayfish host but does not encyst following the trophont stage. Trophonts transform into swimming tomont stages, w...
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journal_title：European journal of protistology
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journal_title：European journal of protistology
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journal_title：European journal of protistology
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journal_title：European journal of protistology
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journal_title：European journal of protistology
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：European journal of protistology
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journal_title：European journal of protistology
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journal_title：European journal of protistology
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journal_title：European journal of protistology
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更新日期：2017-08-01 00:00:00
