Mechanistic insight in potential dual role of sinigrin against Helicoverpa armigera.

Abstract:

:The adverse effect of glucosinolates on diverse phytophagous insects is well documented, but its impact on insect physiology has remained enigmatic. Here we report insights into detrimental effects of plant glucosinolate molecule, sinigrin, on Helicoverpa armigera growth and development. In-silico screening of multiple glucosinolates predicted sinigrin as one of the potential inhibitor of H. armigera cathepsin B and L. Insects fed on sinigrin containing diet showed significantly reduced growth (20-30%), delayed pupation (10-15%), decreased fecundity (50-80%) and developmental abnormalities. Further, sinigrin showed 50-60% inhibition of ex-vivo cathepsin like activity which might be a reason for growth and development related abnormalities. In-vitro and mass spectrometry studies highlighted the cytotoxicity caused due to the hydrolysis of sinigrin, into toxic isothiocyanates, in presence of H. armigera whole body extract. In conclusion, insect cathepsin inhibition and isothiocyanate mediated cytotoxicity lead to the dual adverse effect of sinigrin on H. armigera.

journal_name

Phytochemistry

journal_title

Phytochemistry

authors

Agnihotri AR,Hulagabali CV,Adhav AS,Joshi RS

doi

10.1016/j.phytochem.2017.10.014

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

121-127

eissn

0031-9422

issn

1873-3700

pii

S0031-9422(17)30340-0

journal_volume

145

pub_type

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