Abstract：
BACKGROUND:Medullary thyroid cancer portends poor survival once liver metastasis occurs. We hypothesize that Notch3 overexpression in medullary thyroid cancer liver metastasis will decrease proliferation and growth of the tumor. METHODS:TT cells were modified genetically to overexpress Notch3 in the presence of doxycycline, creating the TT-Notch3 cell line. Mice were injected intrasplenically with either TT-Notch3 or control vector TT-TRE cells. Each cell line had 3 treatment groups: control with 12 weeks of standard chow, early DOX with doxycycline chow at day 0 and for 70 days thereafter, and late DOX with doxycycline chow at 8 weeks. Each animal underwent micro-computed tomography to evaluate for tumor formation and tumor quantification was performed. Animals were killed at 12 weeks, and the harvested liver was stained with Ki-67, hematoxylin and eosin, and Notch3. RESULTS:Induction of Notch3 did not prevent formation of medullary thyroid cancer liver metastases as all mice in the early DOX group developed tumors. However, induction of Notch after medullary thyroid cancer liver tumor formation decreased tumor size, as seen on micro-computed tomography scans (late DOX group). This translated to a 37-fold decrease in tumor volume (P = .001). Notch3 overexpression also resulted in decreased Ki-67 index (P = .038). Moreover, Notch3 induction led to increased areas of neutrophil infiltration and necrosis on hematoxylin and eosin staining of the tumors CONCLUSION: Notch3 overexpression demonstrates an antiproliferative effect on established metastatic medullary thyroid cancer liver tumors and is a potential therapeutic target in treatment.
journal_name
Surgeryjournal_title
Surgeryauthors
Lou I,Odorico S,Yu XM,Harrison A,Jaskula-Sztul R,Chen Hdoi
10.1016/j.surg.2017.07.039subject
Has Abstractpub_date
2018-01-01 00:00:00pages
104-111issue
1eissn
0039-6060issn
1532-7361pii
S0039-6060(17)30677-3journal_volume
163pub_type
杂志文章相关文献
SURGERY文献大全abstract：BACKGROUND:The influence of diabetic therapies on myocardial substrate selection during cardiac surgery is unknown but may be important to ensure optimal surgical outcomes. We hypothesized that metformin and insulin alter myocardial substrate selection during cardiac surgery and may affect reperfusion cardiac function....
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2017.10.020
更新日期：2018-02-01 00:00:00
abstract：:Serious complications of local venous hypertension occur rarely in the upper extremity. These are most frequently the result of a dysfunctional arteriovenous fistula and may produce changes indistinguishable from those of chronic lower-extremity venous insufficiency. A combination of arterial and venous contrast imagi...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
abstract：:Tony is a practicing frontline National Health Service surgeon and director of medical innovation at Anglia Ruskin University and has founded 4 medical-technology start-ups. He has also cofounded the £500 million Anglia Ruskin MedTech Campus, which will become one of the world's largest health innovation spaces. In 20...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2016.07.039
更新日期：2017-05-01 00:00:00
abstract：BACKGROUND:Although task training on virtual reality (VR) simulators has been shown to transfer to the operating room, to date no VR curricula have been described for advanced laparoscopic procedures. The purpose of this study was to develop a proficiency-based VR technical skills curriculum for laparoscopic colorectal...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2011.08.005
更新日期：2012-03-01 00:00:00
abstract：:Twenty-six cases of carcinoid-related mesenteric angiopathy and intestinal infarction (three from our institution and 23 previously reported cases) were reviewed. Twenty patients presented with acute abdominal findings, including peritonitis (13 cases), intestinal obstruction (five cases), and bleeding per rectum (two...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1981-10-01 00:00:00
abstract：:Arteriosclerotic aneurysm of the innominate artery is an unusual lesion. A review of the literature has revealed many approaches to this problem in the past, but the mortality rate has remained significant and serious complications have been frequent. Computerized axial tomography and noninvasive cerebrovascular metho...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1979-05-01 00:00:00
abstract：:Pancreatic polypeptide (PP) deficiency has been associated with impaired hepatic sensitivity to insulin and pancreatogenic diabetes in chronic pancreatitis. Since pancreatic resection might also result in PP deficiency, hepatic responses to insulin infusion (0.25 mU/kg/min) were determined by the euglycemic glucose cl...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1988-08-01 00:00:00
abstract：BACKGROUND:Physiologic and psychological stress are commonly experienced by operating room (OR) personnel, yet there is little research about the stress levels in OR teams and their impact on performance. Previously published procedures to measure physiologic activation are invasive and impractical for the OR. The purp...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2015.04.009
更新日期：2015-11-01 00:00:00
abstract：:The relationship between the cyclosporine A (CSA) dose and rejection of varying lengths of small-bowel grafts was studied in a rat heterotopic microsurgical small-bowel transplantation model involving a haploidentical strain combination. When Lewis X Brown Norway F1 hybrid (LBN) small-bowel grafts were transplanted in...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：:No new chemotherapy agents have been developed recently that present hope for improving survival in patients with colon or rectal cancer. We undertook this study to investigate a new route of administering an old drug, 5-fluorouracil (5-FU). Sixty-six patients with advanced primary colon or rectal cancer were randomiz...
journal_title：Surgery
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1985-09-01 00:00:00
abstract：BACKGROUND:The role of histocompatibility between donor and recipient in liver transplant rejection is unclear because of a paucity of data. The influence of human leukocyte antigen (HLA) type on immunologic graft loss was examined for primary liver transplantations performed at this center. METHODS:Immunologic graft ...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:Lymphocytes from melanoma patients more frequently inhibited the growth of human melanoma cells in the hamster cheek pouch than did lymphocytes from normal donors. Growth inhibition was accomplished with a much lower ratio of target cells to lymphocytes than in in vitro assays. The hamster cheek pouch assay can be use...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：BACKGROUND:Our ability to predict respiratory compromise during general anesthesia in a child with an anterior mediastinal mass is limited. Two prior reports have found a correlation between adequacy of ventilation during general anesthesia and the tracheal cross-sectional area obtained from computed tomograms (compute...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/s0039-6060(05)80360-5
更新日期：1995-09-01 00:00:00
abstract：:Patients with primary and recurrent carcinomas of the breast were studied by the human tumor stem cell assay to determine if (1) colonies would form from breast cancer specimens, (2) growth in the culture would equate with aggressiveness of disease, (3) the assay would yield specific information on drug responsiveness...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1983-08-01 00:00:00
abstract：BACKGROUND:In clinically node-negative patients with differentiated thyroid cancer (DTC), indications for routine central lymph node dissection (RCLD) are the subject of intensive research, and surgeons are divided between the pros and cons of this surgery. To better define the role of neck dissection in the treatment ...
journal_title：Surgery
pub_type： 杂志文章,多中心研究
doi：10.1016/j.surg.2014.02.010
更新日期：2014-06-01 00:00:00
abstract：BACKGROUND:Previous evaluations of the oncologic efficacy of minimally invasive approaches to total gastrectomy in gastric adenocarcinoma have been limited by sample size and duration of follow-up. METHODS:We queried the National Cancer Database to identify patients undergoing robotic and laparoscopic or open total ga...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2019.05.041
更新日期：2019-10-01 00:00:00
abstract：BACKGROUND:The benefit of utilizing surgical safety checklists has been recently questioned. We evaluated our checklist performance after implementing a program that includes checklist-related good catches. METHODS:Multifaceted interventions aimed at the preincision checklist and 5 prospective audits were conducted fr...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2016.04.038
更新日期：2016-12-01 00:00:00
abstract：:A 20-year-old man underwent a 9-hour operation in a left lateral position for the removal of an osteoblastoma involving the pedicles of thoracic vertebrae T-11 and T-12 and for fixation with bond grafting. Immediately after the operation, extensive swelling of the left shoulder area was noted. The radial pulse on the ...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:Serial thickness measurements of the glomerular basement membrane were performed over a 24-month period in four groups of inbred male Lewis rats. Group I consisted of normal animals age-matched to the remaining experimental groups. In groups II, III, and IV, diabetes was induced by intravenous alloxan (42 to 44 mg/kg)...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1980-07-01 00:00:00
abstract：BACKGROUND:This study aimed to determine the prognostic value of a novel grading system based on the histologic assessment of poorly differentiated clusters (PDCs) in the primary lesions in patients with hepatectomy for colorectal liver metastasis (CRLM). Preoperative survival predictors for CRLM are required to determ...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2014.12.025
更新日期：2015-05-01 00:00:00
abstract：:Chronic granulomatous disease (CGD) is a disorder of polymorphonuclear leukocytes that can cause multiple recurrent hepatic abscesses in 40% of those patients with the disorder. The mortality rate from this complication of CGD is estimated at 27%. Treatment has consisted of extensive surgical debridement and drainage ...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1986-05-01 00:00:00
abstract：:Infusion phlebitis is the most common complication of intravenous therapy. Six methods of reducing the incidence of infusion phlebitis including inline final filtration, buffers, heparin, hydrocortisone, heparin-hydrocortisone combinations, and frequent set changes were tested in a two part randomized prospective doub...
journal_title：Surgery
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1979-04-01 00:00:00
abstract：:To determine whether intraoperative urinary output was predictive of postoperative renal function, mean urinary output and lowest hourly urinary output were measured in 137 patients during operation for aortic reconstruction. Pulmonary capillary wedge pressure was kept within normal limits. If urinary output was less ...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1984-06-01 00:00:00
abstract：BACKGROUND:Chronic pancreatitis remains a challenging inflammatory disease, causing progressive, irreversible fibrosis of the pancreatic parenchyma. Several surgical options for treatment have been established, but duodenum-preserving pancreatic head resections seem to be the most favorable option other than pancreatod...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2018.11.009
更新日期：2019-05-01 00:00:00
abstract：BACKGROUND:Health care costs continue to spiral; aggregate costs for 1992 are $850 billion, 13.6% of the U.S. gross national product. The issue has assumed national proportions, and change appears to be on the federal agenda. Great interest in surgical expenditures exists, which comprise 30.1% of all health care outlay...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：BACKGROUND:In living donor liver transplantation, variations in donor vascular anatomy, recipient portal vein thrombosis, short donor vessel length, and reconstitution of the anterior sector outflow in right lobe grafts often make complex and innovative vascular reconstructions necessary, which require the use of exten...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2007.10.008
更新日期：2008-07-01 00:00:00
abstract：BACKGROUND:Sudden massive release of serotonin, histamine, kallikrein, and bradykinin is postulated to cause an intraoperative carcinoid crisis. The exact roles of each of these possible agents, however, remain unknown. Optimal treatment will require an improved understanding of the pathophysiology of the carcinoid cri...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2018.04.093
更新日期：2019-01-01 00:00:00
abstract：:Ten cases of vascular compression of the duodenum are presented, and 125 cases in the literature since 1962 are reviewed. More common in women than in men, the lesion may result from supine immobilization, wearing body cast, or a rapid weight loss. In about one third of the patients, no predisposing factor can be foun...
journal_title：Surgery
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
abstract：INTRODUCTION:Retrograde distal pancreatectomy with splenectomy is the standard procedure for cancers of the body and tail of the pancreas. However, this procedure has limitations in terms of the posterior extent of resection and the ability to achieve a complete N1 node resection. METHODS:A new antegrade procedure has...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1067/msy.2003.146
更新日期：2003-05-01 00:00:00
abstract：HYPOTHESIS:If (1) 3-hydroxy-3-methylglutaryl-coenzyme reductase inhibitors (statins) block the rate-limiting step in cholesterol synthesis and promote the expression of low-density lipoprotein receptors, (2) "Gram-negative sepsis" results from an abundant systemic response to bacterial lipopolysaccharide (endotoxin), (...
journal_title：Surgery
pub_type： 杂志文章
doi：10.1016/j.surg.2005.08.029
更新日期：2006-03-01 00:00:00
