Abstract:
:Chikungunya virus (CHIKV) is a mosquito-borne virus that causes epidemics widely in the world especially in the tropical and subtropical regions. Phylogenetic analysis has found that the CHIKV lineages were associated with the spatial and temporal distributions, which were related to the virus adaption to the major mosquito species and their distributions. In this study, we reported the complete genome sequences of eight CHIKV isolates from the outbreak in Pakistan last year. Then we reviewed the evolutionary history using extensive phylogenetic analysis, analyzed lineage-specific substitutions in viral proteins, and characterized the spreading pathway of CHIKV strains including the Pakistani strains. The results showed that the Pakistani stains belonged to the ECSA.IOL sub-lineage and derived from India. The genetic properties of the Pakistani strains including the adaptive substitution to vectors were further characterized, and the potential risks from the occurrence of CHIKV infection in Pakistan were discussed. These results provided better understanding of CHIKV evolution and transmission in the world and revealed the possible origination of the CHIKV outbreak and epidemic in Pakistan, which would promote the disease prevention and control in the identified countries and territories with the history of CHIKV infections as well as new regions with potential risk of CHIKV outbreaks.
journal_name
Virol Sinjournal_title
Virologica Sinicaauthors
Shi J,Su Z,Fan Z,Wang J,Liu S,Zhang B,Wei H,Jehan S,Jamil N,Shen S,Deng Fdoi
10.1007/s12250-017-4077-5subject
Has Abstractpub_date
2017-12-01 00:00:00pages
520-532issue
6eissn
1674-0769issn
1995-820Xpii
10.1007/s12250-017-4077-5journal_volume
32pub_type
杂志文章abstract::Shiga toxin B-subunit (STxB) from Shigella dysenteriae targets in vivo antigen to cancer cells, dendritic cells (DC) and B cells, which preferentially express the globotriaosylceramide (Gb3) receptor. This pivotal role has encouraged scientists to investigate fusing STxB with other clinical antigens. Due to the challe...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-013-3286-9
更新日期:2013-02-01 00:00:00
abstract::Lepidopteran heat-tolerant (ht) cell lines have been obtained with sf-9, sf-21 and several Bombyx cells. They have a distinct karyotype, membrane lipid composition, morphology and growth kinetics from the parental cell lines. In this paper, we report the development of ht cell lines from other insect species and exami...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-011-3177-x
更新日期:2011-06-01 00:00:00
abstract::Feasible and effective cell models for hepatitis B virus (HBV) infection are required for investigating the complete lifecycle of this virus, including the early steps of viral entry. Resistance to dimethyl sulfoxide/polyethylene glycol (DMSO/PEG), hNTCP expression, and a differentiated state are the limiting factors ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-017-3983-x
更新日期:2017-12-01 00:00:00
abstract::Ebola virus (EBOV) and Marburg virus (MARV), belonging to the Filoviridae family, emerged four decades ago and caused severe viral hemorrhagic fever in human and other primates. As high as 50-90% mortality, filoviruses can cause significant threats to public health. However, so far no specific and efficient vaccine ha...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-011-3186-9
更新日期:2011-04-01 00:00:00
abstract::Histone deacetylase (HDAC) inhibitors show clinical promise for the treatment of cancers, including hepatocellular carcinoma (HCC). In this study, we investigated the effect of HDAC inhibitor treatment on hepatitis C virus (HCV) replication in Huh7 human liver cells and in a mouse model of HCV infection. Viral replica...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-018-0057-7
更新日期:2018-10-01 00:00:00
abstract::Bat SARS-like coronavirus (SL-CoV) has a genome organization almost identical to that of SARS-CoV, but the N-terminus of the Spike (S) proteins, which interacts with host receptor and is a major target of neutralizing antibodies against CoVs, of the two viruses has only 63-64% sequence identity. Although there have be...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-013-3292-y
更新日期:2013-04-01 00:00:00
abstract::Avian infectious bronchitis virus (IBV) is a Gammacoronavirus in the family Coronaviridae and causes highly contagious respiratory disease in chickens. Innate immunity plays significant roles in host defense against IBV. Here, we explored the interaction between IBV and the host innate immune system. Severe histopatho...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-015-3696-y
更新日期:2016-02-01 00:00:00
abstract::In this study, the coding region of type O FMDV capsid protein VP1 and a series of codon optimized DNA sequences coding for VP1 amino acid residues 141-160 (epitope1), tandem repeat 200-213 (epitope2 (+2)) and the combination of two epitopes (epitope1-2) was genetically cloned into the prokaryotic expression vector pP...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-010-3041-4
更新日期:2010-02-01 00:00:00
abstract::In this paper, in vitro anti-influenza virus activities of sulfated polysaccharide fractions from Gracilaria lemaneiformis were investigated. Cytotoxicities and antiviral activities of Gracilaria lemaneiformis polysaccharides (PGL), Gracilaria lemaneiformis polysaccharide fraction-1 (GL-1), Gracilaria lemaneiformis po...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-010-3137-x
更新日期:2010-10-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is causally related to Kaposi's sarcoma (KS), primary effusion lymphoma (PEL) and a proportion of cases of multicentric Castleman's disease (MCD). The ORF73 protein was cloned into pQE80L-orf73 and expressed in E.coli and purified. The expressed recombinant ORF73 was iden...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-010-3106-4
更新日期:2010-06-01 00:00:00
abstract::Conventional influenza vaccines need to be designed and manufactured yearly. However, they occasionally provide poor protection owing to antigenic mismatch. Hence, there is an urgent need to develop universal vaccines against influenza virus. Using nucleoprotein (NP) and extracellular domain of matrix protein 2 (M2e) ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-019-00138-9
更新日期:2019-10-01 00:00:00
abstract::Dengue virus (DENV) and Zika virus (ZIKV) have spread throughout many countries in the developing world and infect millions of people every year, causing severe harm to human health and the economy. Unfortunately, there are few effective vaccines and therapies available against these viruses. Therefore, the discovery ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-018-0068-4
更新日期:2018-12-01 00:00:00
abstract::Southern rice black-streaked dwarf virus (SRBSDV) is a novel Fijivirus prevalent in rice in southern and central China, and northern Vietnam. Its genome has 10 segments of double-stranded RNA named S1 to S10 according to their size. An isolate of SRBSDV, JNi4, was obtained from naturally infected maize plants from Ji'...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-011-3170-9
更新日期:2011-02-01 00:00:00
abstract::A 62-year-old man had chronic hepatitis B virus (HBV) infection and was diagnosed with liver cirrhosis. At the time of diagnosis the patient's virologic markers were positive for hepatitis B surface antigen (HBsAg), antibody to hepatitis B e antigen (anti-HBe) and antibody to hepatitis B core antigen (anti-HBc), while...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-012-3284-3
更新日期:2012-12-01 00:00:00
abstract::Human endogenous retrovirus W env (HERV-W env) plays a critical role in many neuropsychological diseases such as schizophrenia and multiple sclerosis (MS). These diseases are accompanied by immunological reactions in the central nervous system (CNS). Microglia are important immunocytes in brain inflammation that can p...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-017-3997-4
更新日期:2017-06-01 00:00:00
abstract::Porcine respiratory disease complex (PRDC) is a serious health problem that mainly affects growing and finishing pigs. PRDC is caused by a combination of viral and bacterial agents, such as porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), Mycoplasma hyopneumoniae (Myh), Actinob...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-014-3471-5
更新日期:2014-08-01 00:00:00
abstract::The multifunctional trans-activator Tat is an essential regulatory protein for HIV-1 replication and is characterized by high sequence diversity. Numerous experimental studies have examined Tat in HIV-1 subtype B, but research on subtype C Tat is lacking, despite the high prevalence of infections caused by subtype C w...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-016-3681-0
更新日期:2016-06-01 00:00:00
abstract::Primate bocaparvovirus (BOV) is a possible cause of respiratory disorders and gastroenteritis in humans. However, the diversity and evolution of these viruses remain largely unknown, despite the identification of a growing number of BOVs in non-human primates (NHPs). Here, we report the identification of a novel BOV (...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-019-00163-8
更新日期:2020-02-01 00:00:00
abstract::Receptors interaction protein 2 (RIP2) is a specific adaptor molecule in the downstream of NOD2. The role of RIP2 during foot-and-mouth disease virus (FMDV) infection remains unknown. Here, our results showed that RIP2 inhibited FMDV replication and played an important role in the activation of IFN-β and NF-ĸB signal ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00322-2
更新日期:2021-01-05 00:00:00
abstract::Severe fever with thrombocytopenia syndrome virus (SFTSV), the causative agent of a febrile human disease, was first identified from central and eastern provinces in China, and later in Japan and South Korea. Hubei Province is one of the major SFTS epidemic areas in the central part of China. This study reported the i...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00289-0
更新日期:2020-09-22 00:00:00
abstract::Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV-1 infected peoples is still undetermined. In this paper, we compared the protective function of the n...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-012-3239-8
更新日期:2012-06-01 00:00:00
abstract::Hepatitis E virus (HEV) is the prototype of the family Hepeviridae and the causative agent of common acute viral hepatitis. Genetically diverse HEV-related viruses have been detected in a variety of mammals and some of them may have zoonotic potential. In this study, we tested 278 specimens collected from seven wild s...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-018-0011-8
更新日期:2018-02-01 00:00:00
abstract::An emerging mosquito-borne arbovirus named Zika virus (ZIKV), of the family Flaviviridae and genus Flavivirus, is becoming a global health threat. ZIKV infection was long neglected due to its sporadic nature and mild symptoms. However, recently, with its rapid spread from Asia to the Americas, affecting more than 30 c...
journal_title:Virologica Sinica
pub_type: 杂志文章,评审
doi:10.1007/s12250-016-3780-y
更新日期:2016-04-01 00:00:00
abstract::An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced antibody reactions specific to subgroup A or B. But gp85 amino acid sequence comparisons indicated that...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-010-3095-3
更新日期:2010-04-01 00:00:00
abstract::HIV-indeterminate Western blotting (WB) results are typically obtained in WB confirmatory assays, and the number of indeterminate samples may increase with the detection of HIV infections, which will present considerable challenges for the management of HIV/AIDS. Nucleic acid detection has been used as a laboratory te...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-019-00130-3
更新日期:2019-08-01 00:00:00
abstract::Newcastle disease virus (NDV) and H9N2 subtype Avian influenza virus (AIV) are two notorious avian respiratory pathogens that cause great losses in the poultry industry. Current inactivated commercial vaccines against NDV and AIV have the disadvantages of inadequate mucosal responses, while an attenuated live vaccine ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00199-1
更新日期:2020-08-01 00:00:00
abstract::Ion channels are membrane proteins that are found in a number of viruses and which are of crucial physiological importance in the viral life cycle. They have one common feature in that their action mode involves a change of electrochemical or proton gradient across the bilayer lipid membrane which modulates viral or c...
journal_title:Virologica Sinica
pub_type: 杂志文章,评审
doi:10.1007/s12250-010-3136-y
更新日期:2010-08-01 00:00:00
abstract::Respiratory syncytial virus (RSV) is the key underlying cause of acute lower respiratory tract infection in infants; however, no licensed vaccine against RSV infection is currently available. This study was undertaken to assess the preventive effect of vaccine on RSV infection. In this metaanalysis, 1,792 published ra...
journal_title:Virologica Sinica
pub_type: 杂志文章,meta分析
doi:10.1007/s12250-015-3630-3
更新日期:2015-10-01 00:00:00
abstract::Effective oral infection is set off by interaction of a group of conserved per os infectivity factors (PIFs) with larval midgut columnar epithelial cells. We constructed pseudotyped viruses by substituting pif1, pif2 or pif3 genes of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) with their homologs from Mamestra...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-018-0014-5
更新日期:2018-04-01 00:00:00
abstract::Porcine pegivirus (PPgV) is a member of the Pegivirus genus in the Flaviviridae family. PPgV is an emerging virus that has been discovered in swine herds in Germany, the United States, China, Poland, Italy, and the United Kingdom, indicating a wide geographical distribution. In this retrospective study, 339 pig serum ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00240-3
更新日期:2020-07-08 00:00:00