Abstract：
:Primary neonatal Balb/c keratinocyte (NEK) cultures grown, using 3T3 feeder cell support in high calcium, serum supplemented medium, were transfected with EJ-Ha-ras or v-fos DNA sequences and pSV2 neo. Several neo resistant clones were isolated and several established cell lines expressing the transfected gene products derived. Two of these lines, Ras 8 and Fos 1, have been examined in detail with respect to their self renewal capacity and differentiation potential in vitro and in vivo. In vitro, both lines (when compared to normal NEK) have an extended probably immortal phenotype, enhanced colony forming efficiency (a measure of in vitro self renewal capacity) and a reduction in growth factor and serum dependence. When grafted onto syngeneic recipients neither cell line is tumourigenic. Instead a histologically abnormal epithelium with no stratum corneum and with features specific to the oncogene expressed is formed. The extent of the histological atypia correlates with the in vitro alterations in cytoskeletal peptides as revealed by 2D PAGE. However despite the gross histological abnormality there is no alteration in the in vivo self renewal capacity (measured as the number of grafted cells required for epidermal reformation) between normal NEK and the Ras 8 or Fos 1 lines; in each case a minimum of 10(5) cells/1.14 cm2 is required before a full thickness epithelium forms.
journal_name
Oncogenejournal_title
Oncogeneauthors
Appleby MW,Greenfield IM,Crook T,Parkinson EK,Stanley MAsubject
Has Abstractpub_date
1989-11-01 00:00:00pages
1323-30issue
11eissn
0950-9232issn
1476-5594journal_volume
4pub_type
杂志文章相关文献
ONCOGENE文献大全abstract：:The products of the proto-oncogenes c-jun and c-fos are known to form a complex in vivo. Complex formation appears to stabilize protein-DNA interactions and is thought to play an important functional role in transcriptional regulation. Here we show that the viral Jun oncoprotein, which differs structurally from cellul...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1989-02-01 00:00:00
abstract：:Bladder cancer is one of the most common causes of death in industrialized countries. New tumor markers and therapeutic approaches are still needed to improve the management of bladder cancer patients. Choline kinase-alpha (ChoKalpha) is a metabolic enzyme that has a role in cell proliferation and transformation. Inhi...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2009.91
更新日期：2009-07-02 00:00:00
abstract：:The transcription factor activator protein-1 (AP-1) has been implicated in a large variety of biological processes including cell differentiation, proliferation, apoptosis and oncogenic transformation. It is thought that the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced AP-1 activity is because of the activation ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1206102
更新日期：2003-01-16 00:00:00
abstract：:Topoisomerase IIalpha (topoIIalpha) is an essential mammalian enzyme that topologically modifies DNA and is required for chromosome segregation during mitosis. Previous research suggests that inhibition of topoII decatenatory activity triggers a G(2) checkpoint response, which delays mitotic entry because of insuffici...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2010.232
更新日期：2010-08-26 00:00:00
abstract：:A new region of the adenovirus E1a protein essential for immortalization and transformation of primary rat kidney cells has been identified. This region is located between amino acid residues 18 to 20 in an N-terminal domain that is not conserved among the various adenovirus serotypes. The transformation defective mut...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:O6-methylguanine-DNA methyltransferase (MGMT) repairs the cytotoxic and mutagenic O6-alkylguanine produced by alkylating agents such as chemotherapeutic agents and mutagens. Recent studies have shown that in a subset of tumors, MGMT expression is inversely linked to hypermethylation of the CpG island in the promoter r...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1207183
更新日期：2003-12-04 00:00:00
abstract：:Mutation induction in directly exposed cells is currently regarded as the main component of the genetic risk of ionizing radiation for humans. However, recent data on the transgenerational increases in mutation rates in the offspring of irradiated parents indicate that the genetic risk could be greater than predicted ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1209723
更新日期：2006-11-30 00:00:00
abstract：:Amplified segments of the long arm of chromosome 12 are frequently observed in human sarcomas. In most cases there are separate amplified regions around the MDM2 and CDK4 genes. Here we show recurrent amplification of a third region encompassing HMGIC, a human architectural transcription factor gene. Reduced amplifica...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1201135
更新日期：1997-06-19 00:00:00
abstract：:EDD (E3 isolated by differential display), located at chromosome 8q22.3, is the human orthologue of the Drosophila melanogaster tumour suppressor gene 'hyperplastic discs' and encodes a HECT domain E3 ubiquitin protein-ligase. To investigate the possible involvement of EDD in human cancer, several cancers from diverse...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1206775
更新日期：2003-08-07 00:00:00
abstract：:Oncostatin M (OM) is a polypeptide cytokine that induces autocrine and paracrine effects on AIDS-Kaposi's sarcoma (KS) cells (Nair et al., Science, 255, 1430-1432, 1992; Miles et al., Science, 255, 1432-1434, 1992). The signalling pathways underlying this activation are largely unknown. We have found that OM binding t...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:Polymorphisms and somatic mutations in Flap Endonuclease 1 (FEN1), an essential enzyme involved in DNA replication and repair, can lead to functional deficiencies of the FEN1 protein and a predisposition to cancer. We identified a FEN1 germline mutation that changed residue E359 to K in a patient whose family had a hi...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2014.19
更新日期：2015-02-12 00:00:00
abstract：:The requirement for cis-acting DNA sequences for transcriptional activity of the human lck type I promoter was investigated in two human cell lines that express type I transcripts, the leukemic T-cell line, Jurkat, and the colon carcinoma line, SW620. Transient transfection assays in Jurkat and SW620 cells revealed ne...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:During tumor initiation, oncogene-induced senescence (OIS) is proposed to limit the progression of preneoplasms to invasive carcinoma unless circumvented by the acquisition of certain tumor suppressor mutations. Using a variety of biomarkers, OIS has been previously reported in a wide range of human and murine precurs...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2011.350
更新日期：2012-03-22 00:00:00
abstract：:A wealth of cytogenetic data has demonstrated that numerous somatic genetic changes are involved in the pathogenesis of human lung cancer. Despite the complexity of the genomic changes observed in these neoplasms, recurrent chromosomal patterns have emerged. In this review, we summarize chromosomal alterations identif...
journal_title：Oncogene
pub_type： 杂志文章,评审
doi：10.1038/sj.onc.1205836
更新日期：2002-10-07 00:00:00
abstract：:Activation of the TAL1 (or SCL) gene, originally identified through its involvement by a recurrent chromosomal translocation, is the most frequent gain-of-function mutation recognized in T-cell acute lymphoblastic leukemia (T-ALL). The TAL1 proteins contain a basic helix - loop - helix (bHLH) motif characteristic of a...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1202889
更新日期：1999-09-02 00:00:00
abstract：:The SNAIL-related zinc-finger transcription factor, SLUG (SNAI2), is critical for the normal development of neural crest-derived cells and loss-of-function SLUG mutations have been proven to contribute to piebaldism and Waardenburg syndrome type 2 in a dose-dependent fashion. While aberrant induction of SLUG has been ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1208505
更新日期：2005-04-28 00:00:00
abstract：:RING-finger proteins play crucial roles in ubiquitination events involved in diverse cellular processes including signal transduction, differentiation and apoptosis. Most of the RING-finger proteins have E3-ubiquitin ligase activity. RNF11 is a small RING-finger protein and harbors a RING-H2 domain and a PY motif that...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1207319
更新日期：2004-03-11 00:00:00
abstract：:The retinoblastoma (Rb) tumor suppressor gene and its close relatives p107 and p130 are best known for their function in the control of cell cycle progression. In recent years, however, a new role for these proteins has been emerging as they have been linked with regulation of terminal differentiation of many tissues ...
journal_title：Oncogene
pub_type： 杂志文章,评审
doi：10.1038/sj.onc.1203244
更新日期：1999-12-20 00:00:00
abstract：:Recent analyses have revealed that RUNX family members play important roles in both normal developmental processes and carcinogenesis. Of the three known RUNX family members, RUNX3 has been shown to be involved in neurogenesis of the dorsal root ganglia, T-cell differentiation and tumorigenesis of gastric epithelium. ...
journal_title：Oncogene
pub_type： 杂志文章,评审
doi：10.1038/sj.onc.1207286
更新日期：2004-05-24 00:00:00
abstract：:Colon cancer is the most aggressive tumor in both men and women globally. As many the chemotherapeutic regimens have adverse side effects and contribute to the resistance and recurrence, therefore, finding novel therapeutic targets and developing effective agents are urgent. Based on the TCGA and GTEx database analysi...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/s41388-020-01467-w
更新日期：2020-10-01 00:00:00
abstract：:We have shown that one of the principle mechanisms of chemotherapy resistance involves the activation of nuclear factor kappa-B (NF-kappaB). In an effort to identify NF-kappaB-regulated chemotherapy response genes, we performed a microarray assay and observed that heparin-binding EGF-like growth factor (HB-EGF) was si...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1209999
更新日期：2007-03-29 00:00:00
abstract：:Protein phosphatase inhibitors are often considered as tumor promoters. Protein phosphatase 1 regulatory subunit 1A (PPP1R1A) is a potent protein phosphatase 1 (PP1) inhibitor; however, its role in tumor development is largely undefined. Here we characterize, for the first time, the functions of PPP1R1A in Ewing sarco...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2017.378
更新日期：2018-02-08 00:00:00
abstract：:The E6 proteins of specific cancer-associated human papillomaviruses (HPVs) complex with and mediate degradation of the cellular anti-oncogene p53 in vitro. A critical property of p53 is its ability to stimulate transcription from promoters containing its recognition sequence. HPV E6, mutant p53 proteins, and several ...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1995-02-02 00:00:00
abstract：:Targeted expression of transgenes is essential for the accurate representation of human disease in in vivo models. Current approaches to generate conditional transgenic mouse models are cumbersome and not amenable to high-throughput analysis since they require de novo generation and characterization of genetically mod...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/s41388-019-1058-1
更新日期：2020-02-01 00:00:00
abstract：:To study the interplay of steroid hormones and oncogenes in the control of endometrial cell proliferation and differentiation we have generated cell lines derived from rat endometrium by expressing the immortalizing oncogenes adeno E1A or SV40 large T antigen. These lines are positive for mesenchymal markers and conta...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:The management of pain and morbidity due to the spreading and growth of cancer within bone remains to be a paramount problem in clinical care. Cancer cells actively transform bone, however, the molecular requirements and mechanisms of this process remain unclear. This study shows that functional modulation of the alph...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1210429
更新日期：2007-09-13 00:00:00
abstract：:SALL4, a zinc-finger transcriptional factor for embryonic stem cell self-renewal and pluripotency, has been suggested to be involved in tumorigenesis. The role of SALL4 in human gastric cancer, however, remains largely unknown. In this study, we demonstrated that SALL4 was aberrantly expressed at both mRNA and protein...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2013.495
更新日期：2014-11-27 00:00:00
abstract：:Wild-type p53 is stabilized and accumulates in the nucleus of DNA damaged cells. The effect of stabilizing p53 is to inhibit cell growth, either through a G1 cell cycle arrest or apoptotic cell death. MDM2 can inhibit p53 activity, in part, by promoting its rapid degradation through the ubiquitin proteolysis pathway. ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1203980
更新日期：2000-11-30 00:00:00
abstract：:Snail is a zinc finger transcription factor that triggers the epithelial-mesenchymal transition (EMT) by directly repressing E-cadherin expression. Snail is required for mesoderm and neural crest formation during embryonic development and has recently been implicated in the EMT associated with tumour progression. In a...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1205416
更新日期：2002-05-09 00:00:00
abstract：:The degree of DNA methylation in the c-fos gene and its vicinity in liver, brain and spleen of mice of different ages was examined using methylation-sensitive restriction endonucleases. The gene had an invariable unmethylated domain from 1.8kb upstream of the cap site to the first intron. The domain was flanked on bot...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
