Abstract:
BACKGROUND:miRNAs play important roles in multiple biological processes, and deregulation has been linked to several human diseases, including cancer. Studying changes in miRNA expression in cancer development is commonly performed in vitro in human cancer cell lines using quantitative polymerase chain reaction (qPCR), a method requiring the use of a robust reference gene that displays stable expression across all samples. MATERIALS AND METHODS:Using the NormFinder software, a selection of commonly used endogeneous controls and miRNAs were tested in six human cancer cell lines to identify for the most suitable gene for use as a reference. RESULTS:The frequently used endogenous control U6B small nuclear RNA (RNU6B) was found to be an unsuitable reference for normalization. The most suitable single endogeneous control identified was miR-25-3p, whereas the best combination of two endogeneous controls was miR-25-3p and miR-93-5p. CONCLUSION:We identified a single and a pair of miRNAs suitable for use as endogenous controls when performing qPCR-based miRNA expression analyses in human cancer cell lines.
journal_name
Cancer Genomics Proteomicsjournal_title
Cancer genomics & proteomicsauthors
DAS MK,Andreassen R,Haugen TB,Furu Ksubject
Has Abstractpub_date
2016-01-01 00:00:00pages
63-8issue
1eissn
1109-6535issn
1790-6245pii
13/1/63journal_volume
13pub_type
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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doi:
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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doi:
更新日期:2004-05-01 00:00:00
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journal_title:Cancer genomics & proteomics
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doi:
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章,评审
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更新日期:2019-11-01 00:00:00
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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更新日期:2006-03-01 00:00:00
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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