Abstract:
:Arrestins are soluble relatively small 44-46 kDa proteins that specifically bind hundreds of active phosphorylated GPCRs and dozens of non-receptor partners. There are binding partners that demonstrate preference for each of the known arrestin conformations: free, receptor-bound, and microtubule-bound. Recent evidence suggests that conformational flexibility in every functional state is the defining characteristic of arrestins. Flexibility, or plasticity, of proteins is often described as structural disorder, in contrast to the fixed conformational order observed in high-resolution crystal structures. However, protein-protein interactions often involve highly flexible elements that can assume many distinct conformations upon binding to different partners. Existing evidence suggests that arrestins are no exception to this rule: their flexibility is necessary for functional versatility. The data on arrestins and many other multi-functional proteins indicate that in many cases, "order" might be artificially imposed by highly non-physiological crystallization conditions and/or crystal packing forces. In contrast, conformational flexibility (and its extreme case, intrinsic disorder) is a more natural state of proteins, representing true biological order that underlies their physiologically relevant functions.
journal_name
Protein Celljournal_title
Protein & cellauthors
Gurevich VV,Gurevich EV,Uversky VNdoi
10.1007/s13238-017-0501-8subject
Has Abstractpub_date
2018-12-01 00:00:00pages
986-1003issue
12eissn
1674-800Xissn
1674-8018pii
10.1007/s13238-017-0501-8journal_volume
9pub_type
杂志文章,评审相关文献
Protein & Cell文献大全abstract::The liver is the largest internal organ in mammals, and is important for the maintenance of normal physiological functions of other tissues and organs. Hepatitis, cirrhosis, liver cancer and other chronic liver diseases are serious threats to human health, and these problems are compounded by a scarcity of liver donor...
journal_title:Protein & cell
pub_type: 杂志文章,评审
doi:10.1007/s13238-011-1023-4
更新日期:2011-03-01 00:00:00
abstract::In the original publication the PDB numbers were not cited. ...
journal_title:Protein & cell
pub_type: 已发布勘误
doi:10.1007/s13238-019-0619-y
更新日期:2019-07-01 00:00:00
abstract::We recently found that growth factor receptor-bound (Grb) protein 14 is a novel physiological modulator of photoreceptor specific cyclic nucleotide-gated channel alpha subunit (CNGA1). Grb14 promotes the CNG channel closure through its Ras-associating (RA) domain. In the current study we show that this RA domain-media...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-011-1115-1
更新日期:2011-11-01 00:00:00
abstract::Macroautophagy is an evolutionarily conserved intracellular degradation system used by life ranging from yeasts to mammals. The core autophagic machinery is composed of ATG (autophagy-related) protein constituents. One particular member of the ATG protein family, Atg7, has been the focus of recent research. Atg7 acts ...
journal_title:Protein & cell
pub_type: 杂志文章,评审
doi:10.1007/s13238-015-0195-8
更新日期:2015-10-01 00:00:00
abstract::Oral mucositis, a severe oral ulceration, is a common toxic effect of radio- or chemoradio-therapy and a limiting factor to using the maximum dose of radiation for effective cancer treatment. Among cancer patients, at least 40% and up to 70%, of individuals treated with standard chemotherapy regimens or upper-body rad...
journal_title:Protein & cell
pub_type: 杂志文章,评审
doi:10.1007/s13238-014-0130-4
更新日期:2015-03-01 00:00:00
abstract::The nervous system is one of the most complicated organ systems in invertebrates and vertebrates. Down syndrome cell adhesion molecule (DSCAM) of the immunoglobulin (Ig) superfamily is expressed widely in the nervous system during embryonic development. Previous studies in Drosophila suggest that Dscam plays important...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-011-1072-8
更新日期:2011-08-01 00:00:00
abstract::Microbial lipopeptides play an important role in apoptosis induction of tumor cells. However, there is little knowledge about the relationship between apoptosis induction and membrane fatty acids. The present study focused on the effects of lipopeptides produced by Bacillus subtilis HSO121 on Bcap-37 cell lines. 3-(4,...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-010-0072-4
更新日期:2010-06-01 00:00:00
abstract::Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a rare hereditary cerebrovascular disease caused by a NOTCH3 mutation. However, the underlying cellular and molecular mechanisms remain unidentified. Here, we generated non-integrative induced pluripotent stem cells...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-019-0608-1
更新日期:2019-04-01 00:00:00
abstract::Sometimes crystals cannot diffract X-rays beyond 3.0 Å resolution due to the intrinsic flexibility associated with the protein. Low resolution diffraction data not only pose a challenge to structure determination, but also hamper interpretation of mechanistic details. Crystals of a 25.6 kDa non-Pfam, hypothetical prot...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-010-0045-7
更新日期:2010-05-01 00:00:00
abstract::Precision medicine emerges as a new approach that takes into account individual variability. Successful realization of precision medicine requires disease models that are able to incorporate personalized disease information and recapitulate disease development processes at the molecular, cellular and organ levels. Wit...
journal_title:Protein & cell
pub_type: 杂志文章,评审
doi:10.1007/s13238-016-0368-0
更新日期:2017-05-01 00:00:00
abstract::The fruit fly, Drosophila melanogaster, is able to discriminate visual landmarks and form visual long-term memory in a flight simulator. Studies focused on the molecular mechanism of long-term memory have shown that memory formation requires mRNA transcription and protein synthesis. However, little is known about the ...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-011-1019-0
更新日期:2011-03-01 00:00:00
abstract::T-cell receptor (TCR)-engineered T cells are a novel option for adoptive cell therapy used for the treatment of several advanced forms of cancer. Work using TCR-engineered T cells began more than two decades ago, with numerous preclinical studies showing that such cells could mediate tumor lysis and eradication. The s...
journal_title:Protein & cell
pub_type: 杂志文章,评审
doi:10.1007/s13238-016-0367-1
更新日期:2018-03-01 00:00:00
abstract::Blastocyst complementation by pluripotent stem cell (PSC) injection is believed to be the most promising method to generate xenogeneic organs. However, ethical issues prevent the study of human chimeras in the late embryonic stage of development. Primate embryonic stem cells (ESCs), which have similar pluripotency to ...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-019-00676-8
更新日期:2020-02-01 00:00:00
abstract::S-Nitros(yl)ation is a ubiquitous redox-based post-translational modification of protein cysteine thiols by nitric oxide or its derivatives, which transduces the bioactivity of nitric oxide (NO) by regulation of protein conformation, activity, stability, localization and protein-protein interactions. These years, more...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-012-2094-6
更新日期:2012-12-01 00:00:00
abstract::Retinoic acid-inducible gene I (RIG-I) is an important pattern recognition receptor that detects viral RNA and triggers the production of type-I interferons through the downstream adaptor MAVS (also called IPS-1, CARDIF, or VISA). A series of structural studies have elaborated some of the mechanisms of dsRNA recogniti...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-012-2088-4
更新日期:2013-02-01 00:00:00
abstract::Human monocyte is an important cell type which is involved in various complex human diseases. To better understand the biology of human monocytes and facilitate further studies, we developed the first comprehensive proteome knowledge base specifically for human monocytes by integrating both in vivo and in vitro datase...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-016-0342-x
更新日期:2017-02-01 00:00:00
abstract::While human induced pluripotent stem cells (hiPSCs) have promising applications in regenerative medicine, most of the hiPSC lines available today are not suitable for clinical applications due to contamination with nonhuman materials, such as sialic acid, and potential pathogens from animal-product-containing cell cul...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-012-2002-0
更新日期:2012-01-01 00:00:00
abstract::Sterol-regulatory element binding proteins (SREBPs) are the key transcriptional regulators of lipid metabolism. The activation of SREBP requires translocation of the SREBP precursor from the endoplasmic reticulum to the Golgi, where it is sequentially cleaved by site-1 protease (S1P) and site-2 protease and releases a...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-020-00753-3
更新日期:2020-07-14 00:00:00
abstract::Coronaviruses are the causative agent of respiratory and enteric diseases in animals and humans. One example is SARS, which caused a worldwide health threat in 2003. In coronaviruses, the structural protein N (nucleocapsid protein) associates with the viral RNA to form the filamentous nucleocapsid and plays a crucial ...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-010-0079-x
更新日期:2010-07-01 00:00:00
abstract::Treatment of latent tuberculosis infection remains an important goal of global TB eradication. To this end, targets that are essential for intracellular survival of Mycobacterium tuberculosis are particularly attractive. Arylamine N-acetyltransferase (NAT) represents such a target as it is, along with the enzymes enco...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-010-0037-7
更新日期:2010-04-01 00:00:00
abstract::The inherent evolvability of promiscuous enzymes endows them with great potential to be artificially evolved for novel functions. Previously, we succeeded in transforming a promiscuous acylaminoacyl peptidase (apAAP) from the hyperthermophilic archaeon Aeropyrum pernix K1 into a specific carboxylesterase by making a s...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-011-1057-7
更新日期:2011-06-01 00:00:00
abstract::Genome editing tools such as the clustered regularly interspaced short palindromic repeat (CRISPR)-associated system (Cas) have been widely used to modify genes in model systems including animal zygotes and human cells, and hold tremendous promise for both basic research and clinical applications. To date, a serious k...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-015-0153-5
更新日期:2015-05-01 00:00:00
abstract::ATP-sensitive potassium channels (KATP) are energy sensors on the plasma membrane. By sensing the intracellular ADP/ATP ratio of β-cells, pancreatic KATP channels control insulin release and regulate metabolism at the whole body level. They are implicated in many metabolic disorders and diseases and are therefore impo...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-018-0530-y
更新日期:2018-06-01 00:00:00
abstract::Osteosarcoma is the most common primary sarcoma of bone, and it is a leading cause of cancer death among adolescents and young adults. However, the molecular mechanism underlying osteosarcoma carcinogenesis remains poorly understood. Recently, cyclin-dependent kinase 6 (CDK6) was identified as an important oncogene. W...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-016-0277-2
更新日期:2016-06-01 00:00:00
abstract::In the original publication the author's name 'Dimitri Lavillete' is published incorrectly. The correct author name should be spelt as 'Dimitri Lavillette' is provided in this correction. ...
journal_title:Protein & cell
pub_type: 已发布勘误
doi:10.1007/s13238-020-00801-y
更新日期:2020-11-09 00:00:00
abstract::CUE domain-containing 2 (CUEDC2) is a protein involved in the regulation of the cell cycle, inflammation, and tumorigenesis and is highly expressed in many types of tumors. CUEDC2 is phosphorylated by Cdk1 during mitosis and promotes the release of anaphase-promoting complex or cyclosome (APC/C) from checkpoint inhibi...
journal_title:Protein & cell
pub_type: 杂志文章,评审
doi:10.1007/s13238-011-1089-z
更新日期:2011-09-01 00:00:00
abstract::Hepatitis C virus (HCV) is a leading cause of liver disease worldwide. Although several HCV protease/polymerase inhibitors were recently approved by U.S. FDA, the combination of antivirals targeting multiple processes of HCV lifecycle would optimize anti-HCV therapy and against potential drug-resistance. Viral entry i...
journal_title:Protein & cell
pub_type: 杂志文章
doi:10.1007/s13238-018-0521-z
更新日期:2019-03-01 00:00:00
abstract::Dendritic cells (DCs) as a rare type of leukocytes play an important role in bridging the innate and adaptive immune system. A subset of DCs, monocyte-derived dendritic cells (moDCs), exists in very low numbers at steady state but become abundant in inflammatory states. These inflammation-associated DCs are potent pro...
journal_title:Protein & cell
pub_type: 杂志文章,评审
doi:10.1007/s13238-012-0042-0
更新日期:2012-07-01 00:00:00
abstract::A chimeric protein called Wallerian degeneration slow (Wld(S)) was first discovered in a spontaneous mutant strain of mice that exhibited delayed Wallerian degeneration. This provides a useful tool in elucidating the mechanisms of axon degeneration. Over-expression of Wld(S) attenuates the axon degeneration that is as...
journal_title:Protein & cell
pub_type: 杂志文章,评审
doi:10.1007/s13238-010-0021-2
更新日期:2010-03-01 00:00:00
abstract::Diabetes mellitus has become one of the most common chronic diseases, thereby posing a major challenge to global health. Characterized by high levels of blood glucose (hyperglycemia), diabetes usually results from a loss of insulin-producing β-cells in the pancreas, leading to a deficiency of insulin (type 1 diabetes)...
journal_title:Protein & cell
pub_type: 杂志文章,评审
doi:10.1007/s13238-012-2055-0
更新日期:2012-10-01 00:00:00