Abstract:
BACKGROUND:DNA methylation mainly affects tumor suppressor genes in the development of hepatocellular carcinoma (HCC). However, sera methylation of specific genes in hepatitis B virus (HBV)-related HCC remains unknown. AIMS:The purpose of this study was to identify methylation frequencies of sera E-cadherin (CDH1), DNA methyltransferase 3b (DNMT3b) and estrogen receptor 1 (ESR1) promoter in HBV-related HCC and analyze the associated clinical significance. METHODS:Methylation-specific PCR was used to determine the frequencies of DNA methylation for CDH1, DNMT3b and ESR1 genes in sera from 183 patients with HCC, 47 liver cirrhosis (LC), 126 chronic hepatitis B (CHB), and 50 normal controls (NCs). RESULTS:Significantly higher frequencies of methylation of CDH1, DNMT3b and ESR1 were found in HBV-related HCC compared with LC, CHB and NCs. Nodule numbers, tumor size and the presence of liver cirrhosis were significantly associated with gene methylation status in HBV-related HCC. Moreover, HBV may have a strong and enhanced effect on the concurrent methylation of CDH1, DNMT3b and ESR1 in HBV-related HCC. More importantly, combined methylation as a biomarker displayed significantly higher diagnostic value than AFP to discriminate HCC from CHB and LC. CONCLUSIONS:Aberrant sera DNA methylation of CDH1, DNMT3b and ESR1 gene promoters could be a biomarker in the early diagnosis of HBV-related HCC.
journal_name
Dig Dis Scijournal_title
Digestive diseases and sciencesauthors
Dou CY,Fan YC,Cao CJ,Yang Y,Wang Kdoi
10.1007/s10620-015-3975-3subject
Has Abstractpub_date
2016-04-01 00:00:00pages
1130-8issue
4eissn
0163-2116issn
1573-2568pii
10.1007/s10620-015-3975-3journal_volume
61pub_type
临床试验,杂志文章abstract::Effects of selective beta-adrenoceptor agonists on rectosigmoid motility during prolonged rectal distension were studied in 12 healthy volunteers in a double-blind, randomized fashion. Continuous distension was performed with a balloon in the proximal part of the rectum. Pressure was recorded by this balloon and by a ...
journal_title:Digestive diseases and sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF01320259
更新日期:1985-06-01 00:00:00
abstract:BACKGROUND:Esophageal stricture is one of the serious adverse events following endoscopic submucosal dissection (ESD). However, optimum preventive techniques are still lacking. AIMS:Our primary objective was to evaluate the incidence of post-ESD esophageal stricture with the application of carboxymethyl cellulose (CMC...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-016-4034-4
更新日期:2016-06-01 00:00:00
abstract:BACKGROUND AND AIMS:The nature and outcomes of infection among patients with cirrhosis in safety-net hospitals are not well described. We aimed to characterize the rate of and risk factors for infection, both present on admission and nosocomial, in this unique population. We hypothesized that infections would be associ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-020-06164-y
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abstract:BACKGROUND:Patients with cirrhosis are at high readmission risk. Using a large statewide database, we evaluated the effect of hospital cirrhosis-related patient volume on 30-day readmissions in patients with cirrhosis. METHODS:We conducted a retrospective study of the Healthcare Cost and Utilization Project State Inpa...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-018-4964-0
更新日期:2018-09-01 00:00:00
abstract:BACKGROUND:The gut is an important target organ of injury during critically ill conditions. Although Gram staining is a common and quick method for identifying bacteria, its clinical application has not been fully evaluated in critically ill conditions. AIMS:This study's aims were to identify patterns of Gram-stained ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-010-1486-9
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abstract:BACKGROUND:Helicobacter pylori vacuolating cytotoxin, VacA, stimulates apoptosis via a mitochondria-dependent pathway. VacA induces apoptosis via activation of the pro-apoptotic B-cell lymphoma (Bcl)-2 family proteins, Bcl-2-associated X protein (Bax) and Bcl-2 homologous antagonist/killer (Bak), while the implication ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-010-1420-1
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abstract:AIMS:The purpose of this retrospective study was to assess clinical outcomes of endoscopic bougie dilation of esophageal strictures after radiation therapy for head and neck cancer, and to assess the risk factors which affect the treatment success. METHODS:Thirty-one patients with esophageal stricture due to radiation...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-011-1875-8
更新日期:2012-02-01 00:00:00
abstract::Patients with chronic epigastric to right upper quadrant pain are often considered to have gallbladder of sphincter of Oddi dysfunction, but standard tests are nondiagnostic. In 62 consecutive patients with this compliant undergoing antroduodenal manometry, we correlated a change on duodenal motility with spasm of the...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF02212692
更新日期:1995-08-01 00:00:00
abstract::Plasma lipids, apoprotein A-I and B in serum and in lipoprotein fractions (VLDL + LDL, HDL2, and HDL3) obtained by preparative ultracentrifugation, as well as postheparin lipoprotein lipase activity (H-TGL and LPL) were evaluated in 17 subjects with primary biliary cirrhosis (stage II and III) subdivided into two grou...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01536666
更新日期:1988-10-01 00:00:00
abstract::In cases of difficult biliary cannulation, transpancreatic sphincterotomy (TPS) can be an alternative approach of biliary access. However, its success and safety profile have not been studied in detail. A systematic review and meta-analysis were performed to study the overall cannulation success and adverse events of ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章,meta分析
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abstract::The role of HBV precore mutations in the spontaneous reactivation of chronic hepatitis B (CHB) is currently unknown. We studied 10 patients with CHB; five were HBeAg+ (group I) and five were anti-HBe+ (group II). All 10 had spontaneous reactivation of CHB as defined by the appearance of clinical symptoms along with an...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF02088138
更新日期:1994-09-01 00:00:00
abstract::Exposure of the stomach for 30 min to acidified sodium taurocholate (TC) (1-20 mM) or sodium salicylate (SA) (10-80 mM) caused a reduction of transmucosal PD and an increase of luminal pH in anesthetized rats, in a concentration-related manner. Acidified aspirin (ASA) (10-80 mM) reduced PD in the same manner, without ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01296714
更新日期:1987-08-01 00:00:00
abstract::To investigate the possible role of pepsin in ulceration induced by hydrogen ion back-diffusion, the ratio of alkali-labile pepsinogen to total pepsinogen was studied during the course of aspirin- and taurocholate-induced gastric ulceration in comparison with the changes in the ion permeability and histological findin...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01338527
更新日期:1980-11-01 00:00:00
abstract:BACKGROUND AND AIMS:Ulcerative colitis (UC) patients with progression of their disease despite optimized medical therapy may warrant "curative" proctocolectomy with end ileostomy or ileo-anal pouch (IPAA) anastomosis. The aim of our study was to assess the incidence of later recurrent ileitis that lead to altering the ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-017-4873-7
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abstract::Despite progress in therapeutic approaches for the elimination of hepatitis C, chronic hepatitis C virus infection remains an important cause of liver disease. Therapeutic intervention with the currently available interferon-based treatment regimens is quite successful, but treatment is difficult to tolerate and is co...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章,评审
doi:10.1007/s10620-006-9484-7
更新日期:2007-04-01 00:00:00
abstract::Gallbladder contractility has been studied in 21 obese patients (greater than 130% ideal weight) and 30 nonobese subjects before and at regular intervals after the administration of a regular solid-liquid meal, and after a low-calorie, low-fat meal used conventionally for weight-loss purposes (Modifast). Gallbladder v...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01536623
更新日期:1988-01-01 00:00:00
abstract::A 24-year-old white woman presented with abdominal discomfort associated with a firm, nonpulsatile hepatic mass. Radiological examination, which included radionuclide liver scan, abdominal ultrasonography, computed tomography, and hepatic angiography, demonstrated a large, globular, multilocular cyst arising from the ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01296059
更新日期:1986-08-01 00:00:00
abstract::Magnifying videoendoscopic observation of a minute lesion with a single IIIL type cryptal orifice was reported. This minute lesion was a single-gland adenoma accompanied histopathologically by a bud of adenomatous gland and was located in the upper part of the mucosa. This finding regarding a pit pattern should make p...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1020143529123
更新日期:2002-10-01 00:00:00
abstract::Theoretically, relative distal common bile duct obstruction due to sphincter of Oddi dysfunction may be a cause of poor gallbladder evacuation observed on quantitative cholescintigraphy. In this study, the relationship of sphincter of Oddi dysfunction to the gallbladder ejection fraction by quantitative cholescintigra...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF02090199
更新日期:1994-02-01 00:00:00
abstract::We describe a 36-year-old man with advanced multiple myeloma (Salmon and Durie stage III) who developed jaundice and severe cholestasis after a first cure with systemic chemotherapy of vincristine, doxorubicin, and oral dexamethasone (VAD). Serology for hepatitis A, B, and C and for CMV was negative. A liver ultrasoun...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1014773512707
更新日期:2002-04-01 00:00:00
abstract::The living conditions of many aboriginal communities in Canada may place their residents at risk for H. pylori infection. Our aims were to determine: (1) the seroprevalence of H. pylori in a traditional Indian community, (2) the clinical relevance of H. pylori infection in this population, and (3) if H. pylori could b...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1026689103952
更新日期:1999-04-01 00:00:00
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF02064375
更新日期:1995-03-01 00:00:00
abstract::Two baboons receiving intramuscular injections of ferric nitrilotriacetate over a two-year period were compared with two control baboons. The results indicate that in iron-overloaded animals: liver iron excess was major (maximal liver iron concentration values of 42 mumol/100 mg dry weight for both animals vs 1.3 +/- ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01296163
更新日期:1987-06-01 00:00:00
abstract:BACKGROUND:The pathogenesis and treatment of ulcerative colitis remain poorly understood. The aim of the present study is to investigate the effects of black cumin (Nigella sativa) oil on rats with colitis. METHODS:Experimental colitis was induced with 1 mL trinitrobenzene sulfonic acid (TNBS) in 40% ethanol by intrac...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-010-1333-z
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01300751
更新日期:1991-02-01 00:00:00
abstract::Viral dynamic studies in chronic hepatitis C virus (HCV) infection indicate a significantly shortened survival of virus-infected cells. Since at the steady state of chronic viral infection, the rate of infected cell elimination equals new cell regeneration, this would imply a high rate of hepatocyte turnover in chroni...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1015800126283
更新日期:2002-07-01 00:00:00
abstract::We reviewed the discharge records of all diabetic ketoacidosis hospitalizations over 30 months for the presence of clinically significant upper gastrointestinal hemorrhage. Of 284 hospitalizations in 193 patients, hemorrhage occurred in 26 hospitalizations (9%) in 25 patients (13%). None required invasive therapy to a...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF02208576
更新日期:1996-01-01 00:00:00
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journal_title:Digestive diseases and sciences
pub_type: 临床试验,杂志文章
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-009-0715-6
更新日期:2009-04-01 00:00:00
abstract:BACKGROUND:Assessment and quantification of actual liver function is crucial in patients with chronic liver disease to monitor disease progression and predict individual prognosis. Mathematical models, such as model for end-stage liver disease, are used for risk stratification of patients with chronic liver disease but...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-014-3250-z
更新日期:2014-12-01 00:00:00