Abstract：
:The intra-erythrocytic apicomplexan Babesia microti is the predominant pathogen that causes human babesiosis, an infectious disease that occurs worldwide. B. microti relies on the antioxidant including thioredoxin system to maintain the redox balance during the erythrocytic stage. In the present study, the full-length B. microti thioredoxin 3 (BmTrx3) gene was cloned, expressed in vitro, and its response to antiprotozoal drugs were tested. The full-length BmTrx3 was 663 bp and contained an intact open reading frame of 567 bp. The encoded polypeptide was 188 amino acids and the predicted molecular weight of the protein was 21.7 kDa. A conserved thioredoxin-like family domain was found in BmTrx3. The expression of BmTrx3 was upregulated on both the third and eighth day post-infection in mice, whereas expression was downregulated during the beginning and later stages. Western blot analysis showed that mouse anti-BmTrx3 serum could recognize the native BmTrx3 in parasite lysates and that the mouse anti-B. microti serum could recognize the recombinant BmTrx3 protein. Immunofluorescence microscopy showed that BmTrx3 localized in the cell cytoplasm of B. microti merozoites in B. microti-infected red blood cells. The results of bovine insulin reduction assay indicated the enzyme activity of the purified recombinant BmTrx3 protein. The anti-malaria drug chloroquine significantly inhibited the expression of BmTrx3, however, another anti-malaria drug qunine, and a known anti-babesiosis drug clindamycin, induced significantly higher upregulation of BmTrx3 mRNA. The results of the present study demonstrate that BmTrx3 is a functional enzyme with antioxidant activity and may be involved in the response of B. microti to anti-parasite drugs.
journal_name
Ticks Tick Borne Disjournal_title
Ticks and tick-borne diseasesauthors
Huang J,Xiong K,Zhang H,Zhao Y,Cao J,Zhou Y,Gong H,Zhou Jdoi
10.1016/j.ttbdis.2018.01.021subject
Has Abstractpub_date
2018-03-01 00:00:00pages
645-653issue
3eissn
1877-959Xissn
1877-9603pii
S1877-959X(17)30477-6journal_volume
9pub_type
杂志文章abstract：:Previous studies revealed that the Pacific Coast tick (Dermacentor occidentalis) is infected occasionally with the agents of Lyme disease (Borrelia burgdorferi) or human granulocytic anaplasmosis (Anaplasma phagocytophilum) and that it is an inefficient experimental vector of B. burgdorferi. The relationship of the pa...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2010.09.004
更新日期：2010-12-01 00:00:00
abstract：:Theileria equi and Babesia caballi are tick-borne protozoan parasites that can cause anemia in horses. In the Philippines, serological detection of these parasites has only been reported in the Northern area (Luzon). In this study, 105 horses from Cebu and Bohol, Philippines were tested using peripheral blood smear ex...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.04.010
更新日期：2018-07-01 00:00:00
abstract：:The entomopathogenic fungus Metarhizium anisopliae is used as a biological pest control agent against various arthropod species, including ticks. However, the efficacy depends on tick species, tick stage and fungus strain. We studied the effect of M. anisopliae on engorged larvae and nymphs of Ixodes ricinus, the most...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2016.03.010
更新日期：2016-07-01 00:00:00
abstract：:Natural enemies of ticks include the parasitoid wasp Ixodiphagus hookeri (Hymenoptera: Encyrtidae). The aim of this preliminary study was to investigate the occurrence of I. hookeri DNA in a community of ticks (Ixodes ricinus, Dermacentor marginatus, Hyalomma marginatum, Haemaphysalis inermis and Rhipicephalus turanic...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2015.01.001
更新日期：2015-04-01 00:00:00
abstract：:Immature Ixodes scapularis infestation and Borrelia burgdorferi infection of wild small mammals were studied from June to October in 2007 and from May to October in 2008 at 71 study sites in a zone where I. scapularis populations and environmental Lyme disease risk are emerging in southwestern Quebec. Seasonal host-se...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2011.03.005
更新日期：2011-12-01 00:00:00
abstract：:Equine piroplasmosis (EP) is an important tick-borne disease of equids, caused by Theileria equi and Babesia caballi. It is endemic in most parts of the world, including Israel, and has clinical and economic consequences. This study was set to evaluate the presence of EP parasites in domestic donkeys and in wild equid...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.101286
更新日期：2020-01-01 00:00:00
abstract：:Tick-borne encephalitis virus (TBEV) is a zoonotic agent causing severe encephalitis in humans. A wide range of animal species could be infected with TBEV in endemic areas. A serological survey of wild animals is effective in identifying TBEV-endemic areas. Safe, simple, and reliable TBEV serodiagnostic tools are need...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2016.03.002
更新日期：2016-07-01 00:00:00
abstract：:Coxiella burnetii is the causative agent of Q fever. In order to explore the occurrence of C. burnetii in ticks, samples were collected from horses, dogs and humans living in a Cuban occidental community. The species most commonly recovered were Amblyomma mixtum (67%), Rhipicephalus sanguineus s.l. (27%) and Dermacent...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2015.08.008
更新日期：2016-02-01 00:00:00
abstract：:Severe fever with thrombocytopenia syndrome virus (SFTSV) has been emerging and has caused many human cases in China, Japan and Korea. Some studies speculated that SFTSV was transmitted with bird migration among these countries. Notably, SFTS cases have been identified in a Chinese island named Dachen which is situate...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.02.009
更新日期：2019-04-01 00:00:00
abstract：:Rhipicephalus sanguineus sensu lato (s.l.) ticks act as intermediate host for a range of canine vector-borne pathogens, including nematodes ranked in the genus Cercopithifilaria. Though being the object of several studies in the last years, information on the distribution of these parasites is still lacking. In this s...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.04.007
更新日期：2017-06-01 00:00:00
abstract：:Lyme borreliosis (LB) is the most frequent vector-borne disease in Germany. For more than 10 years, data from mandatory notifications have been available from 6 federal states in the eastern part of Germany. A common case definition was applied. Clinical manifestations of erythema migrans, neuroborreliosis (radiculone...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2013.10.010
更新日期：2014-04-01 00:00:00
abstract：:A novel species within the Borrelia burgdorferi sensu lato complex, Borrelia mayonii, was recently described and found to be associated with Lyme borreliosis in the Upper Midwest of the United States. The blacklegged tick, Ixodes scapularis, is naturally infected with B. mayonii in the Upper Midwest and has been exper...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2016.11.002
更新日期：2017-01-01 00:00:00
abstract：:This study was aimed to know epidemiological aspects of Borrelia spp. in a protected urban area of Buenos Aires city, Argentina, where thousands of people visit this area for recreational purposes. Ticks were collected from vegetation, birds and dogs. Three hundred and forty birds belonging to 43 species, 41 genera, 1...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.101282
更新日期：2019-10-01 00:00:00
abstract：:Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potent...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.01.001
更新日期：2017-03-01 00:00:00
abstract：:Thymol is a monoterpene present in plants of the families Lamiaceae, Verbenaceae and Apiaceae. Despite its proven acaricidal activity, little is known about the mechanism of action of thymol in ticks. Thus, the aim of this study was to perform a morpho-histochemical analysis of the synganglion and salivary glands of p...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.11.003
更新日期：2019-02-01 00:00:00
abstract：:Acaricide resistance is one of the greatest threats towards the successful control of vector ticks worldwide. Communal farmers of Zimbabwe use amitraz as the most common acaricide with occasional usage of pyrethroids and organophosphates. As a strategy towards developing an effective acaricide resistance management sy...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.10.017
更新日期：2018-01-01 00:00:00
abstract：:Babesiosis is an emerging tick-borne infection. We describe 2 hospitalized patients from the United States with spontaneous splenic rupture attributable to Babesia microti infection. The first patient was a 36-year-old previously healthy man with a low-grade splenic rupture not requiring surgical intervention. The sec...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2011.08.001
更新日期：2011-12-01 00:00:00
abstract：:Lumpy skin disease (LSD) is caused by lumpy skin disease virus (LSDV), a member of the genus Capripoxvirus. Transmission of the virus has been associated with haematophagous insects such as Stomoxys calcitrans as well as Aedes and Culex species of mosquitoes. Recent studies have reported the transmission of the virus ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2013.09.010
更新日期：2014-03-01 00:00:00
abstract：:Ticks were collected from wild birds at 3 locations in Paraguay during the South American winter in August and September 2012. In total, 480 birds belonging to 106 species were examined. Overall, 31 (6.5%) birds representing 21 species were found parasitized by ticks which were identified as Amblyomma calcaratum Neuma...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2013.08.004
更新日期：2014-03-01 00:00:00
abstract：:Human rickettsioses caused by Rickettsia parkeri strain Atlantic rainforest in Brazil motivated the analysis of Amblyomma ovale ticks in Misiones province, a similar ecological region in northeastern Argentina. During 2010-2017, 393 A. ovale ticks were collected from domestic and wild animals and from vegetation, and ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.05.007
更新日期：2018-07-01 00:00:00
abstract：:Rickettsia parkei is the etiological agent of spotted fever in Uruguay, where is transmitted to humans by the tick Amblyomma triste. In the present study, ticks were collected from capybaras (Hydrochoerus hydrochaeris) and domestic dogs during 2011-2012 in different parts of Uruguay. Three out of 11 (27.3%) Amblyomma ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2014.04.021
更新日期：2014-10-01 00:00:00
abstract：:Attempts to eliminate Rhipicephalus microplus from Uruguay have been unsuccessful, and, currently, the country is divided into two areas: a tick-free area and a tick-infested area. In the tick-infested area, different farms face different situations. Some farms are in regions where, due to environmental conditions or ...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.04.009
更新日期：2019-06-01 00:00:00
abstract：:To date, relatively little is known about the economic and medical impact of Lyme borreliosis (LB) on European health care systems, especially for the inpatient sector. This retrospective analysis is based on data provided for the years 2007-2011 by a German statutory health insurance company (DAK-Gesundheit) covering...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2014.09.004
更新日期：2015-02-01 00:00:00
abstract：:The current study compared the virulence of conidia and blastospores of Metarhizium robertsii (IP 146), M. anisopliae sensu lato (s.l.) (IP 363) and Beauveria bassiana s.l. (IP 361 and CG 307) against unfed larvae and engorged females of Rhipicephalus microplus (Acari: Ixodidae). In addition, the development of fungal...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2018.06.001
更新日期：2018-07-01 00:00:00
abstract：:There are 4 major human-biting tick species in the northeastern United States, which include: Amblyomma americanum, Amblyomma maculatum, Dermacentor variabilis, and Ixodes scapularis. The black bear is a large mammal that has been shown to be parasitized by all the aforementioned ticks. We investigated the bacterial i...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2012.12.002
更新日期：2013-04-01 00:00:00
abstract：:Rodents are hosts of numerous pathogenic agents of public health importance globally. Their ability to harbor these pathogens without showing overt clinical signs of disease has epidemiologic consequences. In some rural settings in Nigeria, humans and rodents do not only share feeds and abode, but the latter may end u...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.10.004
更新日期：2018-01-01 00:00:00
abstract：:Tick-borne encephalitis (TBE) is the most important viral infection transmitted by ticks in Central Europe. In Germany, where TBE was classified as a notifiable disease in 2001, a highly variable number of clinically apparent human cases was reported in the last few years, ranging from the lowest number of 238 in 2007...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2009.11.002
更新日期：2010-03-01 00:00:00
abstract：:Complement is a key first line innate host defense system in the blood of vertebrates. Upon activation, this powerful defense mechanism can elicit inflammatory responses, lyse non-self-cells, or mark them for opsonophagocytic removal. Blood-feeding arthropods thus require the ability to block host complement activatio...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2019.101354
更新日期：2020-03-01 00:00:00
abstract：BACKGROUND:Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is increasingly emerging tool for identification of arthropods including tick vectors using whole or body part of specimens. The challenges of the present study were to assess MALDI-TOF MS profiling for the both ident...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2015.04.013
更新日期：2015-07-01 00:00:00
abstract：:Lyme borreliosis (LB) is the most important human tick-borne disease, but Borrelia genospecies cause different clinical manifestations. Ticks of the genus Ixodes removed from humans between 2006 and 2012 were analysed for Borrelia burgdorferi sensu lato (sl) infections. The majority of ticks originated from the Greate...
journal_title：Ticks and tick-borne diseases
pub_type： 杂志文章
doi：10.1016/j.ttbdis.2017.05.003
更新日期：2017-08-01 00:00:00
