Abstract:
:The aim of the present study was to investigate the prokaryotic community structure of the anaerobic ciliate, Metopus sp. using rRNA sequencing, fluorescence in situ hybridization (FISH), and transmission electron microscopy (TEM). Metopus sp. was physically separated from anaerobic granular sludge in a domestic wastewater treatment plant and anoxically cultivated for 7 d. 16S rRNA gene sequences from the prokaryotes Methanoregula boonei and Clostridium aminobutyricum were abundantly detected in Metopus ciliates. The FISH analysis using the oligonucleotide probes Mg1200b and Cla568 demonstrated that these prokaryotes were localized within Metopus cells. These results identify M. boonei- and C. aminobutyricum-like prokaryotes as novel endosymbionts of Metopus ciliates.
journal_name
Microbes Environjournal_title
Microbes and environmentsauthors
Hirakata Y,Oshiki M,Kuroda K,Hatamoto M,Kubota K,Yamaguchi T,Harada H,Araki Ndoi
10.1264/jsme2.ME15154subject
Has Abstractpub_date
2015-01-01 00:00:00pages
335-8issue
4eissn
1342-6311issn
1347-4405journal_volume
30pub_type
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