Abstract:
:The objective of the current study was to assess the effects of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) along with their simultaneous application on proliferation and pluripotency genes of murine adipose tissue-derived mesenchymal stem cells (AT-MSCs). The proliferation, doubling time (DT), colony-forming unit-fibroblast (CFU-F), pluripotency genes expression, and proliferation-related immunomodulatory markers of MSCs were analyzed upon activation with TNF-α (10 ng/ml), IFN-γ (10 ng/ml) and both TNF-α and IFN-γ (5 ng/ml + 5 ng/ml). Pluripotency genes including Oct-4, Sox-2, and Nanog as well as proliferation-associated immunomodulatory cytokines such as insulin-like growth factor 1 (IGF-1) and transforming growth factor-β (TGF-β) expression were evaluated using real-time PCR. Surface expression of Qa2 (HLA-G) was analyzed by flow cytometry. Pretreatment of MSCs with TNF-α plus IFN-γ led to significantly increased proliferation, DT and CFU-F as well as expression of pluripotency genes in AT-MSCs (p < 0.01). MSCs expressed more IGF-1, TGF-β, and Qa2 upon activation with TNF-α plus IFN-γ and IFN-γ. MSCs expressed significantly decreased amounts of TGF-β and Qa2 in presence of TNF-α. TNF-α combined with IFN-γ may be improved the proliferation of AT-MSCs. Conversely, expanded MSCs pointed out low levels of the immunomodulatory marker, s especially Qa2 in the presence of TNF-α. In conclusion, we showed that TNF-α together with IFN-γ increased the proliferation of MSCs and slightly enhanced the expression of pluripotency genes.
journal_name
Immunopharmacol Immunotoxicoljournal_title
Immunopharmacology and immunotoxicologyauthors
Mohammadpour H,Pourfathollah AA,Nikougoftar Zarif M,Hashemi SMdoi
10.3109/08923973.2015.1115519subject
Has Abstractpub_date
2016-01-01 00:00:00pages
68-76issue
2eissn
0892-3973issn
1532-2513journal_volume
38pub_type
杂志文章abstract::Tubeimoside-1 (TBMS1) is one of the important components in Bolbostemma paniculatum (Maxim.) Franque. In the study, its immunosuppressive effects on murine T lymphocyte responses were evaluated in vitro and in vivo. The data showed that TBMS1 inhibited ConA-induced T lymphocyte proliferation, decreased the ratio of CD...
journal_title:Immunopharmacology and immunotoxicology
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abstract::Context: Pyocyanin is a typical Pseudomonas aeruginosa virulence factor, a common Gram-negative rod responsible for a wide range of severe nosocomial infections. There is evidence indicating that pyocyanin has multiple biological activities, but little is known about anti-inflammatory properties. Objective: This study...
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journal_title:Immunopharmacology and immunotoxicology
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abstract::To determine how estrogen exacerbates allergies, the effects of 17beta-estradiol (E2) on lymphocyte proliferation were investigated. BALB/c mice were ovariectomized, administered 3.2 microg E2, and sensitized with 50 microL 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one. After 7 days, their spleens were excised and flow ...
journal_title:Immunopharmacology and immunotoxicology
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更新日期:2010-06-01 00:00:00
abstract::Contribution of cytotoxic T lymphocytes (CTL) to experimental autoimmune thyroiditis (EAT) was well defined (Speidel et al., Eur. J. Immunol. 1997, 27, 2391-2399, Ref. 7). The native porcine thyroglobulin (pTg) showed high sensitivity to endo-o-N-acetylglucosaminidase F (Endo F) and its molecular weights, correspondin...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章,收录出版
doi:10.1081/iph-200026872
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journal_title:Immunopharmacology and immunotoxicology
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doi:10.3109/08923979109019693
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journal_title:Immunopharmacology and immunotoxicology
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doi:10.3109/08923979709007663
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journal_title:Immunopharmacology and immunotoxicology
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journal_title:Immunopharmacology and immunotoxicology
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journal_title:Immunopharmacology and immunotoxicology
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doi:10.3109/08923979209009231
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journal_title:Immunopharmacology and immunotoxicology
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journal_title:Immunopharmacology and immunotoxicology
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doi:10.3109/08923979809034818
更新日期:1998-08-01 00:00:00
abstract::We have developed an in vitro system to model the interactions of drugs used to treat transplant rejection. This system consists of stimulation of human lymphocytes with a primary mitogen (anti-T-cell receptor complex antibodies (OKT3 or wt31)) and treatment with a primary immunosuppressive drug (ISD) (Cyclosporine A ...
journal_title:Immunopharmacology and immunotoxicology
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doi:10.3109/08923979409007089
更新日期:1994-05-01 00:00:00
abstract::A human THP-1 monocyte cell line culture system has been utilized to observe the effect of granulocyte macrophage colony stimulating factor (GM-CSF) supplementation with lipopolysaccharide (LPS) of oral microorganisms to stimulate monocyte/macrophage functional activity. LPS of oral microorganisms, Fusobacterium nucle...
journal_title:Immunopharmacology and immunotoxicology
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doi:10.3109/08923979809031512
更新日期:1998-11-01 00:00:00
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pub_type: 杂志文章
doi:10.3109/08923973.2014.880717
更新日期:2014-04-01 00:00:00
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journal_title:Immunopharmacology and immunotoxicology
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doi:10.3109/08923979909007140
更新日期:1999-11-01 00:00:00
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journal_title:Immunopharmacology and immunotoxicology
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doi:10.3109/08923973.2014.968256
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journal_title:Immunopharmacology and immunotoxicology
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journal_title:Immunopharmacology and immunotoxicology
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journal_title:Immunopharmacology and immunotoxicology
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journal_title:Immunopharmacology and immunotoxicology
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更新日期:2011-09-01 00:00:00
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journal_title:Immunopharmacology and immunotoxicology
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更新日期:2012-12-01 00:00:00
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journal_title:Immunopharmacology and immunotoxicology
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更新日期:1998-02-01 00:00:00
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journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
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更新日期:2002-11-01 00:00:00
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