Abstract:
:A bacterial concentration fluorescence immunoassay (BCFIA) was developed to rapidly detect periodontopathic bacteria in human plaque samples. The BCFIA utilized fluorescent-tagged monoclonal antibodies (MAbs) directed against the lipopolysaccharide of selected Gram-negative bacteria. Microorganisms identified in plaque using the BCFIA included Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, and Fusobacterium nucleatum. The immunoassay procedure involved combining a patient's plaque sample with a species-specific fluorescein isothiocyanate-labeled MAb and then incubating the mixture in a specialized microtiter plate allowing the MAb to bind to its homologous bacteria. Bound and unbound fluorescent-tagged MAbs were separated by filtration and total bound bacterial fluorescence was determined with a fluorimeter. The relative number of a bacterial species in a given plaque sample was estimated by reference to a standard curve carried through the BCFIA. The BCFIA had a lower detection limit of near 104 specific bacterial cells in a mixed bacterial preparation or plaque sample. When compared to cultivable flora procedures in detecting the 4 periodontopathogens, the BCFIA had high levels of statistical sensitivity, 97% to 100%, while statistical specificity ranged between 57% and 92%. There was a 71% to 82% agreement between BCFIA and DNA probe methodology in detecting periodontopathogens in plaque. The BCFIA, when compared to cultivable flora, offers the advantage of evaluating both live and dead bacterial cells in plaque. This may in part, if not fully, explain the lower specificity values of the BCFIA when compared to cultivable flora. Screening plaque samples for periodontopathic bacteria is considerably faster and results in a greater frequency of detection with BCFIA than cultivable flora based methods. J Periodontol 1992; 63:1093-1101.
journal_name
J Periodontoljournal_title
Journal of periodontologyauthors
Wolff LF,Anderson L,Sandberg GP,Reither L,Binsfeld CA,Corinaldesi G,Shelburne CEdoi
10.1902/jop.1992.63.12s.1093subject
Has Abstractpub_date
1992-12-01 00:00:00pages
1093-1101eissn
0022-3492issn
1943-3670journal_volume
63 Suppl 12Spub_type
杂志文章abstract:BACKGROUND:The aim of this split-mouth study is to compare long-term (18 to 35 years) periodontal conditions of sites treated with gingival augmentation procedures (GAPs) and untreated homologous contralateral sites. METHODS:Forty-seven patients with 64 sites (test group), with lack of attached gingiva associated with...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2016.160284
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:Macrophages account for 5% to 30% of the inflammatory infiltrate in periodontitis and are activated by the classic and alternative pathways. These pathways are identified by indirect markers, among which interferon (IFN)-γ and interleukin-6 (IL)-6 of the classic pathway and IL-4 of the alternative pathway ha...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2013.130078
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND:In a previous study, recombinant human transforming growth factor-beta1 (rhTGF-β1 ) in a calcium carbonate carrier was implanted into critical-size, supraalveolar periodontal defects under conditions for guided tissue regeneration (GTR) to study whether rhTGF-β1 would enhance or accelerate periodontal regene...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2007.060336
更新日期:2007-04-01 00:00:00
abstract:BACKGROUND:Because of bisphosphonate medication, dental implantation with a subsequent infection poses a relevant risk factor to suffer from medication-related osteonecrosis of the jaw. This rat study evaluated different implant materials under systemic bisphosphonate delivery using micro-computed tomography (μCT) imag...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.20-0547
更新日期:2020-11-30 00:00:00
abstract::Proposing to place endosseous implants is an integral facet of dental treatment plans. Their insertion is usually associated with a low incidence of untoward events. However, despite careful planning, surgical complications can arise: infection, intraoral hemorrhage, wound dehiscence, postoperative pain, lack of prima...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.2008.070067
更新日期:2008-08-01 00:00:00
abstract::Matrix metalloproteinases (MMP) are a family of proteolytic enzymes that mediate the degradation of extracellular matrix macromolecules, including interstitial and basement membrane collagens, fibronectin, laminin, and proteoglycan core protein. The enzymes are secreted or released in latent form and become activated ...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.1993.64.5s.474
更新日期:1993-05-01 00:00:00
abstract::The clinical periodontal status of 44 insulin-dependent diabetic children and adolescents and 20 healthy control subjects was compared for a period of approximately 5 years. Fasting blood glucose, fructosamine, and glycosylated hemoglobin (HbA1) values were determined at baseline and 5 years later. The differences in ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1997.68.2.136
更新日期:1997-02-01 00:00:00
abstract::Ten mouse monoclonal antibodies were prepared against cultured bovine periodontal ligament cells to be used as reagents for the study of periodontal disease and wound healing. Using standard immunohistochemical methods, these antibodies were found to recognize cell surface antigens in formalin-fixed bovine periodontiu...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1991.62.3.190
更新日期:1991-03-01 00:00:00
abstract::Public health surveillance has been defined as the ongoing systematic collection, analysis, interpretation, and dissemination of data regarding a health-related event for use in public health action to reduce morbidity and mortality and to improve health. Surveillance is an essential element of public health program i...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.2007.060340
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:Little information is available about the effects of the cessation of cigarette smoking on oral health, although cigarette smoking has been shown to be associated with a variety of oral diseases. The aim of this study was to compare periodontal status, salivary proteolytic activity, especially collagenase-2 ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1999.70.11.1361
更新日期:1999-11-01 00:00:00
abstract:BACKGROUND:Members of the matrix metalloproteinase (MMP) family have been shown to be involved in periodontal disease. Risk factors for periodontal disease include tobacco smoking. Cigarette smoke condensate (CSC) is comprised of thousands of chemicals. Nicotine is one of the active components in tobacco. This study co...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2010.100540
更新日期:2011-07-01 00:00:00
abstract::The purposes of this study were to determine if: 1) an association exists between cigarette smoking and signs of periodontal disease after controlling for the confounding variables of age, sex, plaque, and calculus; 2) the prevalence of 5 bacteria commonly associated with periodontal disease differs between smokers an...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1993.64.12.1225
更新日期:1993-12-01 00:00:00
abstract:BACKGROUND:Little attention has been given to understanding the variation in biomechanical behavior of oral soft tissues, and this represents an obstacle for the development of biomaterials that perform with appropriate biomechanical characteristics. With this as our motivation, a uniaxial mechanical analysis was perfo...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2011.100573
更新日期:2011-08-01 00:00:00
abstract::Repeated measurements of attachment level appear to be important assessments in periodontal clinical trials, yet the lack of reliability for this assessment creates measurement error which in turn demands increased sample sizes or reduces the power of the test. A plastic occlusal stent has been developed as a fixed re...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1987.58.2.115
更新日期:1987-02-01 00:00:00
abstract::To date, there are no ultrastructural studies that have examined untreated chronic periodontal pockets immediately following dental debridement with an oral irrigation device. This study used both scanning electron microscopic and transmission electron microscopic methodology to examine previously untreated human peri...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1988.59.3.155
更新日期:1988-03-01 00:00:00
abstract:BACKGROUND:Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia (previously T. forsythensis), which are regarded as the principal periodontopathogenic bacteria, exist as a consortium in subgingival biofilms. We aimed to examine quantitative relationships between P. gingivalis, T. denticola, and T. fo...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2008.070398
更新日期:2008-04-01 00:00:00
abstract:SCOPE:These Guidelines apply to products used to promote the regeneration of periodontal tissues lost due to periodontal diseases or other causes. Periodontal regeneration is defined histologically as regeneration of the tooth's supporting tissues, including alveolar bone, periodontal ligament, and cementum over a prev...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1998.69.9.1081
更新日期:1998-09-01 00:00:00
abstract:BACKGROUND:The aim of this study was to assess the long-term success and survival rates of implants replacing a single molar between two natural teeth and to evaluate the influence of implant characteristics on implant success. METHODS:The study was based on a consecutive cohort of 81 patients who received implants to...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2006.060005
更新日期:2006-09-01 00:00:00
abstract:BACKGROUND:Evaluation of clinical long-term results 20 years after connective tissue grafting (CTG) or guided tissue regeneration (GTR) using bioabsorbable barriers for root coverage therapy. METHODS:Initially, 15 patients with 38 Miller Class I and II recession defects underwent CTG or GTR according to random assignm...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.19-0203
更新日期:2020-03-01 00:00:00
abstract:BACKGROUND:β-glucuronidase (βG) is one of the enzymes involved in the destruction of non-collagenous components of the extracellular matrix. It is also considered an indicator or predictor of periodontal disease activity. The present study was conducted to determine the presence and the levels of βG activity in gingiva...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2000.71.4.618
更新日期:2000-04-01 00:00:00
abstract:BACKGROUND:It has been proposed that the presence of a zone of keratinized mucosa (KM) around implants is associated with less discomfort during brushing and improved esthetic outcomes. Therefore, mucogingival procedures have been recommended for patients with discomfort during brushing, and to enhance esthetic results...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.18-0031
更新日期:2018-09-01 00:00:00
abstract::In previous studies Fusobacterium nucleatum has been shown to induce either stimulatory or inhibitory effects on human mononuclear cells. We examined the interaction of human mononuclear cells with human and cynomolgus monkey strains of F. nucleatum. Peripheral blood mononuclear cells (PBMCs) isolated from normal dono...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1997.68.1.39
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Multiple factors that influence the precision of periodontal probing measurements have been identified. These factors can be categorized as being dependent upon host-related, probe-related, or examiner-related variables. However, the potential influence of examiner handedness (right or left) on the measureme...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2006.050185
更新日期:2006-07-01 00:00:00
abstract:BACKGROUND:Prevalence of peri-implantitis is directly proportional to the time of functional loading. The aim of this retrospective study was to assess the survival and success rates as well as the incidence of peri-implantitis among patients with a history of periodontitis and among implants with 1 to 10 years of func...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.18-0715
更新日期:2019-12-20 00:00:00
abstract::The literature related to smoking and periodontal disease is reviewed. The effects of smoking on oral hygiene, gingivitis, necrotizing gingivitis, periodontitis, bacteria and the host's response are presented. From this review it is apparent that while the relationship between smoking and periodontal disease needs fur...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.1986.57.10.617
更新日期:1986-10-01 00:00:00
abstract:BACKGROUND:Arachidonate-5-lipoxygenase (5-LO) activity and increased leukotriene B4 (LTB4) production have been implicated in various inflammatory conditions. Increased production of leukotrienes has been associated with periodontal diseases; however, their relative contribution to tissue destruction is unknown. In thi...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2017.170210
更新日期:2017-12-05 00:00:00
abstract:BACKGROUND:To date only a few studies have evaluated the long-term influence of smoking and smoking cessation on periodontal health. The present study, therefore, was undertaken with the aim to prospectively investigate the influence of smoking exposure over time on the periodontal health condition in a targeted popula...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2000.71.8.1338
更新日期:2000-08-01 00:00:00
abstract::The study design of national surveys of periodontal disease often uses partial-mouth examination protocols to rationalize the need for resources so that the collection of data from a large number of subjects can be achieved within the available means without significantly sacrificing precision and validity. Studies sh...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2011.100638
更新日期:2011-03-01 00:00:00
abstract::In order to provide longitudinal data for endosteal bladevent implants, a continuing study is being conducted from an original group of 49 implants in 32 patients placed 15 years ago. The present work evaluated 26 of these bladevent implants in 18 patients. Three basic patterns were observed 15 years postoperatively: ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1987.58.9.589
更新日期:1987-09-01 00:00:00
abstract::The objective of this study was to evaluate if the biologic membrane utilized for GTR can be impregnated by tetracycline hydrochloride and if the chemotherapeutic agent, once impregnated, can be released in minimal inhibitory concentrations for a period compatible with clinical application. Initially, an in vitro stud...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1995.66.11.978
更新日期:1995-11-01 00:00:00