Abstract:
:The aim of the current study was to investigate the effects of FL118, a novel camptothecin analogue, on migration and invasion of human breast cancer cells and the underlying mechanisms of those effects. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and a plate clone formation assay were used to examine inhibition of the proliferation of MDA-MB-231 cells by FL118. Cell cycle distribution was detected using flow cytometry. A wound healing assay and a transwell assay were performed to detect the effects of FL118 on migration and invasion of MDA-MB-231 cells, respectively. qRT-PCR, Western blotting, and immunocytochemistry were used to study the effects of FL118 on expression of epithelial-mesenchymal transition (EMT)-related molecules and Wnt/ β-catenin signaling components in MDA-MB-231 cells. The current results indicated that FL118 inhibited the proliferation, migration and invasion of MDA-MB-231 cells in a dose- and time-dependent manner. FL118 caused MDA-MB-231 cells to accumulate in the S phase. FL118 significantly suppressed the expression of vimentin while enhancing the expression of E-cadherin. Moreover, decreased expression of β-catenin and its targets survivin and cyclin Dl was detected in the nucleus of MDA-MB-231 cells. Taken together, the current results suggest that FL118 inhibited Wnt/β-catenin signaling, leading to suppressed EMT and decreased migration and invasion of breast cancer cells.
journal_name
Biosci Trendsjournal_title
Bioscience trendsauthors
Yang Z,Ji L,Jiang G,Liu R,Liu Z,Yang Y,Ma Q,Zhao Hdoi
10.5582/bst.2017.01288subject
Has Abstractpub_date
2018-01-01 00:00:00pages
40-46issue
1eissn
1881-7815issn
1881-7823journal_volume
12pub_type
杂志文章abstract::Hepatitis B induced by hepatitis B virus (HBV) remains a major public health problem worldwide. Although several antiviral drugs have been approved for hepatitis B, they cause significant dose-dependent side-effects (interferon-alpha) and drug resistance (lamivudine, etc.). Safe and potent new anti-HBV drugs are urgen...
journal_title:Bioscience trends
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journal_title:Bioscience trends
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journal_title:Bioscience trends
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Bioscience trends
pub_type: 杂志文章,评审
doi:
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pub_type: 杂志文章,评审
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journal_title:Bioscience trends
pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章,多中心研究
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