Abstract:
INTRODUCTION:Endothelial dysfunction has become a strongly discussed factor regarding glaucoma pathogenesis. In addition to peripapillary bleedings as signs of vascular damage, there is a definite correlation between glaucoma and vascular dysregulation syndrome. The aim of this study was to evaluate endothelial cell reaction to moderately elevated hydrostatic pressure and oxidative stress in vitro. METHODS:In vitro, primarily dissociated brain microvascular endothelial cells (BMECs) were exposed to moderately elevated hydrostatic pressure (60 and 120 mmHg) in a special pressure chamber. Additionally, cells primarily exposed to pressure, and cells not exposed to pressure, were incubated with low amounts of H2O2. A live/dead assay was performed to evaluate cell viability. Immunohistochemical staining against actin was used for morphological evaluation. RESULTS:Neither 60 nor 120 mmHg of elevated pressure had a viability changing effect on primary endothelial cells. Secondary, no big morphological changes could be discovered. However, against a low concentration of oxidative stress, BMECs showed high vulnerability. A difference in reaction to cells stressed with high pressure before could not be shown. CONCLUSION:Direct effects, in terms of higher vulnerability or morphological changes of moderately elevated high pressure on endothelial cells, could not be shown. However, the reaction to low amounts of oxidative stress indicates the involvement of endothelial cells in the pathogenesis of glaucoma and the special role of oxidative stress when referring to endothelial dysfunction in glaucomatous disease. HINTERGRUND:Im Rahmen der Pathogenese des Glaukoms wird die endotheliale Dysfunktion zunehmend diskutiert. Peripapilläre Blutungen sind diagnostisch wegweisend. Die Korrelation von Glaukomerkrankungen mit vaskulärem Dysregulationssyndrom ist eindeutig. Ziel dieser Studie ist es, die genaue Reaktion der Endothelzellen auf erhöhten hydrostatischen und oxidativen Stress zu untersuchen. MATERIAL UND METHODEN:In vitro wurden primär dissoziierte BMECs (brain microvascular endothelial cells) für 3 Tage normalem und leicht erhöhtem hydrostatischem Druck von 60 und 120 mmHg in einer Druckkammer ausgesetzt. Zusätzlich wurden sowohl druckbelastete als auch nicht druckbelastete Zellen oxidativem Stress in Form von geringen Konzentrationen H2O2 ausgesetzt. Ein Live/Dead Assay wurde durchgeführt, um die Zellviabilität zu messen. Morphologisch wurden die Zellen mit immunhistochemischer Aktinfärbung beurteilt. ERGEBNISSE:Interessanterweise zeigten die Endothelzellen sowohl unter 60 mmHg als auch unter 120 mmHg kein vermehrtes Absterben im Vergleich zu den Zellen ohne Belastung. Auch morphologisch zeigten sich keine großen Unterschiede. Gegenüber oxidativem Stress wurden alle Zellen schon bei kleinen Mengen geschädigt. Keinen Unterschied konnte man zwischen oxidativem Stress ohne vorherige Druckbelastung und oxidativem Stress mit vorheriger Druckbelastung von 120 mmHg für 3 Tage feststellen. SCHLUSSFOLGERUNG:Wir konnten keinen direkten Effekt in Form von vermehrtem Zelluntergang der Endothelzellen auf erhöhten hydrostatischen Druck feststellen. Allerdings zeigt die Reaktion auf die geringen Konzentrationen von oxidativem Stress, dass die Zellen im Rahmen der Pathogenese des Glaukoms doch in Mitleidenschaft gezogen werden. Der oxidative Stress scheint hier eine besondere Rolle zu spielen.
journal_name
Klin Monbl Augenheilkdjournal_title
Klinische Monatsblatter fur Augenheilkundeauthors
Mann C,Thanos S,Brockhaus K,Grus FH,Pfeiffer N,Prokosch Vdoi
10.1055/s-0043-122677subject
Has Abstractpub_date
2019-09-01 00:00:00pages
1122-1128issue
9eissn
0023-2165issn
1439-3999journal_volume
236pub_type
杂志文章abstract:PURPOSE:Fireworks combusted during New Year's Eve festivities can cause different eye traumas which often need complex reconstructive surgery. It was our aim to systematically analyse these eye trauma cases which were treated at our clinic during the last eight years. MATERIALS AND METHODS:Age, gender, side, trauma me...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-0034-1368376
更新日期:2014-09-01 00:00:00
abstract:INTRODUCTION:Chronic hepatitis C-therapy is limited to the combined use of PEG-ylated interferons and ribavirin. Side effects of this therapy include retinal changes that manifest with bleeding, cotton wool spots and/or thrombosis. PATIENT:A 51-year-old man presented with a sudden decrease of visual acuity. Chronic he...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2004-813607
更新日期:2004-09-01 00:00:00
abstract:BACKGROUND:Pseudoexfoliation syndrome is a frequent cause of secondary open-angle glaucoma due to deposition and in situ-production of abnormal extracellular matrix proteins in the trabecular meshwork. Glaucomas itself are a known risk factor for retinal vein thrombosis. In this retrospective study the prevalence of ps...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1035088
更新日期:1997-07-01 00:00:00
abstract::Localised distichiasis and trichiasis with resulting keratopathy leads to considerable impairment for the patient. We present a new technique which enables the surgeon to excise the lash with its follicle to prevent regrowth in a single movement. The procedure does not need any preparation, leaving the lid with minima...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-0032-1327905
更新日期:2013-01-01 00:00:00
abstract::As a result of an acute pathologic event ophthalmic arterial pressure may fall below a level necessary to supply sufficient blood to the eye and optic nerve, and thus to preserve full visual function. A sudden partial or even total loss of visual function. A sudden partial or even total loss of vision may follow. In s...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1057432
更新日期:1980-02-01 00:00:00
abstract:BACKGROUND:Infections with varicella zoster virus may involve the optic nerve and the retina. Different pathomechanisms have been discussed. We present a case with an autoimmune inflammatory reaction according to the clinical course. PATIENT:A 69-year-old female was referred to our clinic because of suspected bilatera...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1034771
更新日期:1999-03-01 00:00:00
abstract::A horizontal diplopia is produced with prisms whose bases are nasal or temporal. The subject has to fixate a circle, now seen double, alternately with his left and right eye and touch the center of the circle with a finger. Of one hundred subjects thus examined, 85 located the circle accurately every time when the one...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1046410
更新日期:1989-07-01 00:00:00
abstract::By means of fluorescein angiography extensive defects in the pigmented epithelium found in choroideremia can be shown to be present in the first ten years of life. The degeneration of the vessels of the choroid and the functional damage to the retina do not seem to take place until later. ...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:
更新日期:1979-04-01 00:00:00
abstract:PURPOSE:Imaging methods of the optic nerve head appear to have an increasing impact in glaucoma diagnosis. The aim of this study is to evaluate the specifity of the Heidelberg Retina Tomograph (software version 1.7 and 3.0) in subjects with physiological cupping and large optic discs. PATIENTS AND METHODS:27 eyes of 2...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-0032-1328094
更新日期:2014-02-01 00:00:00
abstract::The present report describes clinical findings in 13 patients with Sturge-Weber syndrome, 11 of whom had glaucoma (22-52 mm Hg). The episcleral venous pressure (EVP) was high in all glaucomatous eyes (14-23 mm Hg). Tonography showed a marked decrease in outflow facility. The observations suggest that glaucoma in Sturg...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1050508
更新日期:1987-10-01 00:00:00
abstract::Upon utilization of echography one certainly will not be able to detect retinal defects; however, other parameters of retinal detachment which by means of solely optical examination techniques may remain undiagnosed can be evaluated successfully. This concerns not only the diagnosis of retinal detachment alone but als...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:
更新日期:1979-03-01 00:00:00
abstract::The position of the implanted posterior chamber lens (Shearing type) in human cadaver eyes was photographed from the rear. We were especially interested in the position of the loops. ...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1057359
更新日期:1981-12-01 00:00:00
abstract::Die Tränendrüse kann von einer Vielzahl neoplastischer, infektiöser und autoimmunologischer Erkrankungen betroffen sein. Eine radiologische Charakterisierung der Veränderungen kann Hinweise für die differenzialdiagnostische Einordnung liefern. Erkrankungen der ableitenden Tränenwege, insbesondere Tränenwegsverschlüsse...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-0033-1358102
更新日期:2015-03-01 00:00:00
abstract:PURPOSE:To evaluate the efficacy of an intravitreal dexamethasone implant (Ozurdex®) in patients with cystoid macular oedema (CME) suffering from non-infectious uveitis. METHODS:Monocentric, retrospective, single-arm analysis in 49 patients (59 implanted eyes) with inactive uveitis, in whom CME had been unresponsive t...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-0042-102058
更新日期:2016-05-01 00:00:00
abstract::There are marked differences between the public health system and the private insurance system. This paper shows how to evaluate the degree of permanent disability according to "The General Rules for Private Insurances (AUVB 1965)". At the beginning the evaluation of the degree of permanent disability after the visus ...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:
更新日期:1979-03-01 00:00:00
abstract::The use of adaptive optics in ophthalmoscopy is a breakthrough technological achievement. With AO ophthalmoscopes, the microscopic retinal structure can be visualised non-invasively and on a cellular level, allowing for cellular scale imaging of the retinal nerve fibre layer, the smallest retinal capillaries, rod and ...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章,评审
doi:10.1055/s-0043-100631
更新日期:2017-03-01 00:00:00
abstract::It has been shown by a series of 127 dacryocystorhinostomies using the Toti technique, that this operation, which is independent of age or aethiology, can be performed using a simple procedure and a short hospitalization time. The success rate of over 90% indicates that this operation should be used as a standard proc...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
abstract:BACKGROUND:Development of a reflectance oximeter to measure non-invasively the changes in oxygen saturation (SO 2 ) in the vessels of the human eye fundus, with the goal of obtaining a better understanding of the retinal circulation in health and disease. MATERIAL AND METHODS:For retinal oximetry, an instrument must i...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2002-30648
更新日期:2002-04-01 00:00:00
abstract:BACKGROUND:The aim of this study was to compare the results of intraocular pressure (IOP) measurements obtained using the pressure phosphene tonometer Proview with those from Goldmann applanation tonometer (GAT) in normal and glaucomatous eyes. PATIENTS AND METHODS:The IOP in 150 eyes of 62 healthy volunteers and 88 p...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2006-927094
更新日期:2006-11-01 00:00:00
abstract::Prophylactic cryo coagulation is especially suitable when there is cloudy media, e.g. blood in the vitreous body or a cataract which would prevent light or laser coagulation. Cryo lesions applied under ophthalmoscopic control are without complication. The paper reviews the 116 eyes collected in 1971 and augments it wi...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:
更新日期:1976-06-01 00:00:00
abstract:BACKGROUND:Subretinal perfluorocarbon liquid (PFCL) droplets are occassionally seen after vitreoretinal surgery for retinal detachment. Usually they are located peripherally without causing complications and therapeutic consequences. In contrast, centrally located PFCL droplets affect final visual outcomes. In animal e...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-0031-1299159
更新日期:2012-07-01 00:00:00
abstract:BACKGROUND:We investigated the presence of Nicotinamid-adenin-dinucleotid-phosphat (NADPH)-diaphorase activity in endothelial cells of the human uveal vessels, using a histochemical procedure which presumably localizes the reaction product nitric oxide. MATERIAL AND METHODS:11 donor eyes destined for corneal transplan...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1035414
更新日期:1995-02-01 00:00:00
abstract:BACKGROUND:The alternating prism and cover test is the conventional test for the measurement of the angle of strabismus. The error induced by the prismatic effect of glasses is typically about 27-30%/10 D. Alternatively, the angle of strabismus can be measured with methods based on Purkinje reflex positions. This study...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1034870
更新日期:1998-04-01 00:00:00
abstract::There are multiple possible intraocular manifestations in patients with systemic neoplasms. At first line there are uveal, retinal and vitreous metastases from carcinomas, melanomas and lymphomas. In patients with von Hippel Lindau syndrome or familiar adenomatous polyposis coli (FAP), Gardner or Turcot syndromes, fun...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章,评审
doi:10.1055/s-0031-1273474
更新日期:2011-07-01 00:00:00
abstract::.25% Bupranolol in an oily solution decreases corneal sensitivity statistically significant from the 3. to the 15. minute after the application. The maximum of decrease is found between the 6. and the 15. minute in contrast to beta-blockers in aqueous solution (1.-3. minute). The topical subjective compatibility of 0....
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1055/s-2008-1045923
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Ocular involvement in panarteritis nodosa (PAN) has been reported to occur in 10 to 20% of patients. In 3 patients with acute visual disturbance we point out unusual findings. PATIENTS:Case 1. A 40-year-old man initially presented with papilledema together with partial optic atrophy in both eyes, later poly...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2001-11260
更新日期:2001-01-01 00:00:00
abstract::A case history of a young man with recurrent papillophlebitis. The fluorescein angiogram showed an extensive hyperfluorescence of the disk and surrounding retinal veins in the venous and late phase. The electro-ophthalmological findings were normal in both eyes, while computerized perimetry demonstrated an enlarged bl...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1055250
更新日期:1982-11-01 00:00:00
abstract:INTRODUCTION:The course of the recovery phase after general anaesthesia, during which consciousness and pain gradually return, is essentially determined by the choice of anaesthetic drug, and its total dose or duration of application. This phase is often complicated by more or less severe agitation, especially in child...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章,随机对照试验
doi:10.1055/a-1260-3023
更新日期:2020-10-01 00:00:00
abstract::Lysosomal storage diseases represent a group of about 50 genetic disorders. The deficiencies of lysosomal and non-lysosomal proteins cause an accumulation of compounds which are normally degraded within the lysosome. There are currently no therapeutic options to cure patients suffering from a lysosomal storage disease...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章,评审
doi:10.1055/s-0028-1109958
更新日期:2011-02-01 00:00:00
abstract::Eighteen patients who underwent surgery for nervus abducens paresis (NAP), were kept under observation for up to 23 years after the operation. Three patients had paresis of both nerves. The operation, employing O'Connor's method (11 patients) included the grafting of split inferior and superior rectus muscles on to th...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1054369
更新日期:1985-11-01 00:00:00