Abstract：
:Mice with targeted gene disruption have provided important information about the molecular mechanisms of circadian clock function. A full understanding of the roles of circadian-relevant genes requires manipulation of their expression in a tissue-specific manner, ideally including manipulation with high efficiency within the suprachiasmatic nuclei (SCN). To date, conditional manipulation of genes within the SCN has been difficult. In a previously developed mouse line, Cre recombinase was inserted into the vesicular GABA transporter (Vgat) locus. Since virtually all SCN neurons are GABAergic, this Vgat-Cre line seemed likely to have high efficiency at disrupting conditional alleles in SCN. To test this premise, the efficacy of Vgat-Cre in excising conditional (fl, for flanked by LoxP) alleles in the SCN was examined. Vgat-Cre-mediated excision of conditional alleles of Clock or Bmal1 led to loss of immunostaining for products of the targeted genes in the SCN. Vgat-Cre+; Clockfl/fl; Npas2m/m mice and Vgat-Cre+; Bmal1fl/fl mice became arrhythmic immediately upon exposure to constant darkness, as expected based on the phenotype of mice in which these genes are disrupted throughout the body. The phenotype of mice with other combinations of Vgat-Cre+, conditional Clock, and mutant Npas2 alleles also resembled the corresponding whole-body knockout mice. These data indicate that the Vgat-Cre line is useful for Cre-mediated recombination within the SCN, making it useful for Cre-enabled technologies including gene disruption, gene replacement, and opto- and chemogenetic manipulation of the SCN circadian clock.
journal_name
J Biol Rhythmsjournal_title
Journal of biological rhythmsauthors
Weaver DR,van der Vinne V,Giannaris EL,Vajtay TJ,Holloway KL,Anaclet Cdoi
10.1177/0748730418757006subject
Has Abstractpub_date
2018-04-01 00:00:00pages
179-191issue
2eissn
0748-7304issn
1552-4531journal_volume
33pub_type
杂志文章abstract：:Although pineal "synaptic" ribbons (SR) are frequently examined by means of quantitative electron microscopy, their functional significance remains unclear. The same is true for related structures--"synaptic" spherules (SPH). In the course of such studies, it has been noted that SR counts may differ from laboratory to...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/074873048800300103
更新日期：1988-04-01 00:00:00
abstract：:DEC1 and DEC2-basic helix-loop-helix transcription factors-exhibit a circadian expression in the suprachiasmatic nucleus and other peripheral tissues and seem to play roles in regulating the mammalian circadian rhythm by suppressing the CLOCK/BMAL1-activated promoters of Per1, Dec1, and Dec2. The authors present data ...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730405280195
更新日期：2005-10-01 00:00:00
abstract：:Honey bee workers care for ("nurse") the brood around the clock without circadian rhythmicity, but then they forage outside with strong circadian rhythms and a consolidated nightly rest. This chronobiological plasticity is associated with variation in the expression of the canonical "clock genes" that regulate the cir...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730411431404
更新日期：2012-02-01 00:00:00
abstract：:The mammalian circadian clock is known to be entrained by both a daily light-dark cycle and daily feeding cycle. However, the mechanisms of feeding-induced entrainment are not as fully understood as those of light entrainment. To elucidate the first step of entrainment of the liver clock, we identified the circadian c...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730411405958
更新日期：2011-06-01 00:00:00
abstract：:Numerous studies have used the classic van der Pol oscillator, which contains a cubic nonlinearity, to model the effect of light on the human circadian pacemaker. Jewett and Kronauer demonstrated that Aschoff's rule could be incorporated into van der Pol type models and used a van der Pol type oscillator with higher o...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/074873099129000867
更新日期：1999-12-01 00:00:00
abstract：:The circadian clock represents an anticipatory mechanism, well preserved in evolution. It has a critical impact on most aspects of the physiology of light-sensitive organisms. These rhythmic processes are governed by environmental cues (fluctuations in light intensity and temperature), an internal circadian timing sys...
journal_title：Journal of biological rhythms
pub_type： 杂志文章,评审
doi：10.1177/0748730414557634
更新日期：2015-04-01 00:00:00
abstract：:A single light episode during the first larval stage can set the phase of adult Drosophila activity rhythms, showing that a light-sensitive circadian clock is functional in larvae and is capable of keeping time throughout development. These behavioral data are supported by the finding that neurons expressing clock pro...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730403260621
更新日期：2004-02-01 00:00:00
abstract：:Nonphotic stimuli can reset and entrain circadian activity rhythms in hamsters and mice, and serotonin is thought to be involved in the phase-resetting effects of these stimuli. In the present study, the authors examined the effect of the serotonin agonist quipazine on circadian activity rhythms in three inbred strain...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/074873099129000524
更新日期：1999-04-01 00:00:00
abstract：:Photic signals affect circadian activity rhythms by both phasic and tonic mechanisms that modulate pacemaker phase and period. In mammals, the effects of light on circadian activity are mediated by the retina, which communicates with the suprahiasmatic nucleus (SCN) by two different anatomical routes: the retino-hypot...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/074873048700200104
更新日期：1987-04-01 00:00:00
abstract：:Entrainability of the circadian rhythm of mosquito flight and locomotor activity was investigated by giving a 24-hr temperature cycle (28 degrees C for 16 hr, 23 degrees C for 8 hr) to the free-running rhythm or by shifting the temperature cycle. The phase angle difference between the entrained rhythm and the temperat...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/074873049300800304
更新日期：1993-10-01 00:00:00
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journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/074873098128999880
更新日期：1998-02-01 00:00:00
abstract：:Crustaceans have frequently been used to study the neuroethology of both agonistic behavior and circadian rhythms, but whether their highly stereotyped and quantifiable agonistic activity is controlled by circadian pacemakers has, so far, not been investigated. Isolated marbled crayfish (Procambarus spec.) displayed r...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730408328933
更新日期：2009-02-01 00:00:00
abstract：:In our attempts to understand the circadian system, we unavoidably rely on abstractions. Instead of describing the behavior of the circadian system in all its complexity, we try to derive basic features from which we form a global concept on how the system works. Such a basic concept is a model of reality. The author ...
journal_title：Journal of biological rhythms
pub_type： 杂志文章,评审
doi：10.1177/0748730405277388
更新日期：2005-08-01 00:00:00
abstract：:Circadian rhythms are programmed by the suprachiasmatic nucleus (SCN), which relies on neuropeptide signaling to maintain daily timekeeping. Vasoactive intestinal polypeptide (VIP) is critical for SCN function, but the precise role of VIP neurons in SCN circuits is not fully established. To interrogate their contribut...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730420925573
更新日期：2020-08-01 00:00:00
abstract：:Reduced sensitivity to short-wavelength (blue) light with age has been shown for light-induced melatonin suppression. The current research aimed to determine if a similar age-related reduction occurs in subjective alertness, mood, and circadian phase-advancing responses. Young (n = 11, 23.0 +/- 2.9 years) and older (n...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730408328973
更新日期：2009-02-01 00:00:00
abstract：:The suprachiasmatic nucleus (SCN) drives and synchronizes daily rhythms at the cellular level via transcriptional-translational feedback loops comprising clock genes such as Bmal1 and Period (Per). Glycogen synthase kinase 3 (GSK3), a serine/threonine kinase, phosphorylates at least 5 core clock proteins and shows diu...
journal_title：Journal of biological rhythms
pub_type： 信件
doi：10.1177/0748730415573167
更新日期：2015-04-01 00:00:00
abstract：:The daily biological clock regulates the timing of sleep and physiological processes that are of fundamental importance to human health, performance, and well-being. Environmental parameters of relevance to biological clocks include (1) daily fluctuations in light intensity and temperature, and (2) seasonal changes in...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730408320284
更新日期：2008-08-01 00:00:00
abstract：:Since the advent of techniques to investigate gene expression on a large scale, numerous circadian rhythms in mRNA amount have been reported. These rhythms generally differ in amplitude and phase. The authors investigated how far a parameter not regulated by the circadian clock can influence the phase of a rhythm in R...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730408325753
更新日期：2008-12-01 00:00:00
abstract：:A physiologically based mathematical model of a putative sleep-wake regulatory network is used to investigate the transition from typical human sleep patterns to spontaneous internal desynchrony behavior observed under temporal isolation conditions. The model sleep-wake regulatory network describes the neurotransmitte...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730413504277
更新日期：2013-10-01 00:00:00
abstract：:The circadian pacemaker of the SCN is a heterogeneous structure containing many single-cell oscillators that display phase differences in gene expression and electrical activity rhythms. Thus far, it is unknown how single neurons contribute to the population signal measured from the SCN. The authors used single-unit e...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730406290317
更新日期：2006-08-01 00:00:00
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journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730404272907
更新日期：2005-04-01 00:00:00
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pub_type： 临床试验,杂志文章
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更新日期：2000-06-01 00:00:00
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journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730404270388
更新日期：2004-12-01 00:00:00
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journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730409336579
更新日期：2009-08-01 00:00:00
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journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730413486873
更新日期：2013-06-01 00:00:00
abstract：:In mammals, the suprachiasmatic nucleus (SCN) is the central pacemaker organizing circadian rhythms of behavior and physiology. At the cellular level, the mammalian clock consists of autoregulatory feedback loops involving a set of "clock genes," including the Cryptochrome (Cry) genes, Cry1 and Cry2. Experimental evid...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730412461246
更新日期：2012-12-01 00:00:00
abstract：:Activity patterns are the product of interactions between an internal circadian clock and direct responses to photic and nonphotic features of the environment that are said to "mask" the influence of that clock. Evolutionary transitions between nocturnality and diurnality involve changes in mechanisms underlying both ...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730409351672
更新日期：2010-02-01 00:00:00
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journal_title：Journal of biological rhythms
pub_type： 杂志文章,评审
doi：10.1177/0748730402239672
更新日期：2003-02-01 00:00:00
abstract：:An intricate transcription-translation feedback loop (TTFL) governs cellular circadian rhythms in mammals. Here, we report that the zinc finger transcription factor Krüppel-like factor 9 (KLF9) is regulated by this TTFL, it associates in chromatin at the core circadian clock and clock-output genes, and it acts to modu...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/0748730420913205
更新日期：2020-06-01 00:00:00
abstract：:A 12-hr dark period, at a temperature high enough to permit time measurement to occur, is necessary for maximal induction of larval diapause in the European corn borer, Ostrinia nubilalis. In the present study, induction of diapause only occurred in a periodic environment. This was in the form of certain (1) light-dar...
journal_title：Journal of biological rhythms
pub_type： 杂志文章
doi：10.1177/074873048600100205
更新日期：1986-07-01 00:00:00
