Abstract:
:Here we show that a labyrinth channel compartment and slit diaphragms, which are the histological structures enabling insect nephrocytes ultrafiltration, are established during embryogenesis first by the garland nephrocytes (GCNs). The later pericardial nephrocytes, which represent the majority of functional nephrocytes in larvae and adults, lack these characteristic features at the embryonic stage. During larval development, a subpopulation of the pericardial cells survives and matures into functional nephrocytes (PCNs) displaying a fully differentiated slit diaphragm and a labyrinth channel compartment. Likely the embryonic pericardial cells have primary functions other than ultrafiltration (e.g. in production and secretion of ECM constituents). We also show, for the first time, that PCNs in the adult fly undergo dramatic histological degeneration upon ageing. The slit diaphragms disappear, the labyrinth channel system degenerates and the lysosomal compartment becomes highly enriched with electron-dense material. When using nephrocytes as a model for genetic screening purposes or to investigate the specific role of genes involved in endocytosis, histological changes occurring upon ageing need to be taken into account when interpreting structural data.
journal_name
Mech Ageing Devjournal_title
Mechanisms of ageing and developmentauthors
Psathaki OE,Dehnen L,Hartley PS,Paululat Adoi
10.1016/j.mad.2018.04.006subject
Has Abstractpub_date
2018-07-01 00:00:00pages
9-20eissn
0047-6374issn
1872-6216pii
S0047-6374(18)30015-0journal_volume
173pub_type
杂志文章abstract::The age-related deficit in the dose response of osteoprogenitor cells to IGF-I was further investigated. As expected, the effective dose, but not the maximal effect, was shifted two orders of magnitude higher in old cells. In this paper, we examined whether this age-deficit can be attributed to an alteration in the ex...
journal_title:Mechanisms of ageing and development
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journal_title:Mechanisms of ageing and development
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journal_title:Mechanisms of ageing and development
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journal_title:Mechanisms of ageing and development
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journal_title:Mechanisms of ageing and development
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journal_title:Mechanisms of ageing and development
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journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
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journal_title:Mechanisms of ageing and development
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journal_title:Mechanisms of ageing and development
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journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
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journal_title:Mechanisms of ageing and development
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journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(78)90039-8
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journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(98)00086-4
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journal_title:Mechanisms of ageing and development
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journal_title:Mechanisms of ageing and development
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journal_title:Mechanisms of ageing and development
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journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
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journal_title:Mechanisms of ageing and development
pub_type: 临床试验,杂志文章
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更新日期:2014-11-01 00:00:00
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journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/j.mad.2018.04.002
更新日期:2019-01-01 00:00:00
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journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2019.01.001
更新日期:2019-03-01 00:00:00
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journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
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journal_title:Mechanisms of ageing and development
pub_type: 临床试验,杂志文章
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abstract::Formalinized Mycoplasma pulmonis was used to immunize 3 different age groups of Fischer 344 rats. A specific antibody to this antigen was detected in both saliva and lung lavage fluids and differences were noted in the elicitation of secretory antibody between the different ages of the animals. Few statistical differe...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(92)90131-v
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abstract::Mycobacteria are an important group of pathogenic bacteria. We generated a series of DNA repair deficient strains of Mycobacterium smegmatis, a model organism, to understand the importance of various DNA repair proteins (UvrB, Ung, UdgB, MutY and Fpg) in survival of the pathogenic strains. Here, we compared tolerance ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2013.08.006
更新日期:2013-10-01 00:00:00