Abstract:
:The presence or absence of minimal residual disease (MRD) in patients with multiple myeloma (MM) has emerged as a useful marker to determine the depth of remission. MRD negativity as an endpoint has been shown to be associated with improved progression-free survival in many studies. MRD detection is therefore part of numerous clinical trial protocols for MM. At the present time, two methodologies are most widely accepted for MRD detection: (1) multicolor flow cytometry and (2) next-generation sequencing-based clonotype detection. While both of those methodologies enable accurate quantification of MRD in the bone marrow (BM), with sensitivity as low as 10-5 to 10-6, there are several limitations to these methods. First, these approaches reveal the presence or absence of MRD but provide limited molecular information about MM. More comprehensive characterization of MM cells at the MRD stage may identify molecular mechanisms of drug resistance. Second, MRD detection in the BM is typically performed at one time point only, but more frequent detection may define the duration of the MRD status and thus refine its prognostic value. Third, less-invasive approaches that avoid the discomfort and risk associated with BM biopsy would be highly desirable, especially in elderly or frail patients. "Liquid biopsy" for the detection and characterization of circulating MM cells may address these issues. Although MRD detection in the peripheral blood at the same sensitivity as in the BM may be challenging, the identification of patients who do not achieve MRD negativity might reduce the need for BM biopsies. Here, we give an overview of approaches that have been described to detect and characterize MM cells when they occur at very low frequencies in the peripheral blood or in the BM, emphasizing recently described next-generation sequencing approaches for more comprehensive characterization of circulating MM cells.
journal_name
Semin Hematoljournal_title
Seminars in hematologyauthors
Waldschmidt JM,Anand P,Knoechel B,Lohr JGdoi
10.1053/j.seminhematol.2018.02.010subject
Has Abstractpub_date
2018-01-01 00:00:00pages
33-37issue
1eissn
0037-1963issn
1532-8686pii
S0037-1963(18)30021-0journal_volume
55pub_type
杂志文章,评审abstract::Continuous daily treatment with all-trans-retinoic acid (ATRA) in patients with acute promyelocytic leukemia has been associated with a marked decline in the plasma drug concentration at the time of relapse. Recent pharmacologic studies have attempted to determine the reasons for the progressive reduction of plasma co...
journal_title:Seminars in hematology
pub_type: 杂志文章,评审
doi:
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journal_title:Seminars in hematology
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abstract::This phase I trial of idarubicin (IDA) was conducted in 32 patients with acute leukemia and chronic myelogenous leukemia (CML) in blastic crisis (CML/BC) who either had failed to achieve a complete remission (CR) after initial induction therapy or had relapsed after CR. IDA was administered at dosages ranging from an ...
journal_title:Seminars in hematology
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journal_title:Seminars in hematology
pub_type: 杂志文章
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journal_title:Seminars in hematology
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journal_title:Seminars in hematology
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journal_title:Seminars in hematology
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journal_title:Seminars in hematology
pub_type: 历史文章,杂志文章
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journal_title:Seminars in hematology
pub_type: 杂志文章,评审
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journal_title:Seminars in hematology
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journal_title:Seminars in hematology
pub_type: 杂志文章,评审
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journal_title:Seminars in hematology
pub_type: 杂志文章,评审
doi:
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journal_title:Seminars in hematology
pub_type: 临床试验,杂志文章,多中心研究
doi:
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pub_type: 杂志文章,评审
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journal_title:Seminars in hematology
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pub_type: 杂志文章,评审
doi:10.1016/s0037-1963(01)90052-6
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journal_title:Seminars in hematology
pub_type: 杂志文章,评审
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journal_title:Seminars in hematology
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